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Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal
Published by Universitas Jenderal Soedirman
ISSN : 08531629     EISSN : 08531629     DOI : -
Core Subject : Science, Agriculture, Social,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science
Majalah Ilmiah Biologi Biosfera : A Scientific Journal merupakan peer reviewed jurnal yang diterbitkan oleh Fakultas Biologi Universitas Jenderal Soedirman. Media ini mewadahi hasil-hasil penelitian di bidang biologi tropika yang terbit tiga kali setahun (Januari, Mei, September).
Arjuna Subject : -
Articles 342 Documents
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Study on The Efficacy of Nitrogen Utilization by Rice Genotypes Part 1: Clustering and Selection for Rice Genotypes Darjanto Darjanto; Didik Indradewa; Bostang Radjagukguk; Taryono Taryono
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 28, No 2 (2011)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2011.28.2.261

Abstract

This experiment was carried out in a greenhouse as, the first part of four consecutive experiments. Sixty rice genotypes comprising 30 national and 30 local genotypes were evaluated for their responses and efficacy to nitrogen (N, urea) fertilizer application. Two levels of N fertilizer, i.e., 0 (N1) and 120 (N2) kg of N per hectare were applied. Randomized Block Design (RBD) with three replications was used as the experimental design. The observed parameters were grain yield per pot and grain yield index (GI). Based on average of grain yield of N1, average of grain yield of N2, and grain yield index (GI), the 60 evaluated genotypes were classified into four clusters. These clusters consisted of 26 efficient and responsive (ER) genotypes, 7 efficient and non-responsive (ENR) genotypes, 6 non-efficient and responsive (NER) genotypes, and non-efficient and non-responsive (NENR) genotypes. Replacement of cultivars in rice cultivation, from non efficient (NE)-genotype to efficient (E)-genotype has a potential of yield increase of 90.83%, replacement of non-responsive (NR) with responsive (R) genotype has a potential yield increase of 59.57%, replacement of local genotypes with national genotypes has a potential to increase yield 8.66%  only.  Of the 26 ERs, genotypes with the highest efficiency were Singkil, IR-66, Indragiri, Sintanur, and Widas.  Genotypes with the highest response were Ciliwung, IR-66, Ciherang, Sintanur, and Cisadane.  These genotypes can be used to increase rice production, to reduce production costs and to reduce environmental pollution. The ER, ENR and NER genotypes can be used as parents in breeding for high yielding and N efficient rice genotypes.
Kandungan Senyawa Penangkal Sinar Ultra Violet dari Ekstrak Rumput Laut Eucheuma cottonii dan Turbinaria conoides Rini Yanuarti; Nurjanah Nurjanah; Effionora Anwar; Ginanjar Pratama
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 34, No 2 (2017)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2017.34.2.467

Abstract

Rumput laut memiliki beberapa aplikasi ekonomi dalam berbagai jenis industri, salah satunya untuk industri kosmetik. Salah satu kosmetik yang banyak digunakan yaitu krim tabir surya. Efektivitas dari krim tabir surya biasanya dinyatakan dengan nilai SPF (sun protection factor). Tujuan dari penelitian ini adalah untuk mengetahui kandungan senyawa aktif dan nilai SPF pada ekstrak rumput laut Turbinaria conoides dan Eucheuma cottonii yang dapat digunakan sebagai bahan aktif pada sediaan krim tabir surya. Hasil pengujian menunjukkan rumput laut Turbinaria conoides mengandung golongan senyawa fenol hidrokuinon, flavonoid, triterpenoid, steroid, dan saponin. Rumput laut Eucheuma cottonii hanya ditemukan senyawa alkaloid. Nilai SPF tertinggi pada rumput laut Turbinaria conoides terdapat pada ekstrak metanol (SPF 16.7), sedangkan untuk rumput laut Eucheuma cottonii terdapat pada ekstrak etil asetat (SPF 8.8). Hasil tersebut memperlihatkan bahwa ekstrak dari rumput laut Turbinaria conoides  dan Eucheuma cottonii memiliki potensi yang baik jika digunakan sebagai bahan aktif pada sediaan krim tabir surya. 
Ular Welang, Bungarus fasciatus (Schneider, 1801), di Lereng Selatan Gunung Merapi, Daerah Istimewa Yogyakarta Donan Satria Yudha; Rury Eprilurahman; M. Fahrul Hilmi; Iman Akbar Muhtianda; Aisyah Arimbi
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 30, No 3 (2013)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2013.30.3.136

Abstract

There are three known kraits species of the Genus Bungarus (Elapidae) inhabit Java Island, Indonesia. Two species out of the three species, i.e., Bungarus candidus (Malayan kraits) and Bungarus fasciatus (Banded kraits) are commonly found in Daerah Istimewa Yogyakarta (DIY) Province. However, a longterm  research about herpetofauna diversity in the Southern slope of Mount Merapi since 2006 until 2011 shows no species records from genus Bungarus. A nocturnal survey on April 28th, 2012 was administerd around the Kaliurang recreational area. We found snake which was suggested belong to the genus Bungarus. Detailed identification showed that this snake was Bungarus fasciatus or banded kraits snake. This finding provide the new record of genus Bungarus in Southern slope of Merapi especially since the periodical research of herpetofauna diversity which was started in 2006. To complete the data, it needs intensive research to know the distribution and species abundance of Bungarus fasciatus in Southern slope of Mount Merapi.
Kajian Kualitas Air Pasca-Pengerukan Alur Pelayaran Batu Kapur Sungai Donan Cilacap Yogi Pamungkas; Sidharta Sahirman; Moh. Husein Sastranegara
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 31, No 1 (2014)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2014.31.1.222

Abstract

Donan River dredging impacts on ecological regions and communities around the basin. Dredging from Buoy 27 to Buoy segment 34 as an effort to reduce silting of existing in Cilacap and Donan River shipping channel maintenance efforts limestone PT Holcim Indonesia Tbk. Cilacap Plant. The purpose of the study is to determine the condition of post-dredging water quality in terms of physical and chemical factors, to determine the condition of post-dredging water quality in terms of biological factors; and to analyze the relationship between physical, chemical, and biological factors post-dredging shipping lanes limestone around Cilacap Donan River. The research method used was purposive sampling method. The study was conducted from March-May 2013, with five replications. Samples were taken at three stations namely Station 1 (area before dredging), Station 2 (dredging area), and Station 3 (area after dredging). The results showed that water quality conditions of post-dredging chemical in terms of physical factors tend to decrease at station 2 (TSS amounted of 303.6 mgl-1, BOD5 9.806 mgl-1, COD 171.4 mgl-1), followed by station 3 (TSS 268 mgl-1, BOD5 at 18.182 mgl-1, COD is 262.8 mgl-1) and station 1 (TSS 204.4 mgl-1, BOD5 at 12.258 mgl-1, and COD of 252 , 2 mgl-1). Water quality conditions in terms of post-dredging tend to decrease at station 2 (the number of types makrobenthos by 5 species, number of individuals ind/m2 makrobenthos by 147, the number of types of plankton by 11 species and number of individuals of 1,461 ind/l) followed by station 3 (the number of species makrobenthos by 6 species, number of individuals ind/m2 makrobenthos by 180, the number of types of plankton by 12 species, and the number of individual plankton of 1,341 ind / l), and Station 1 (the number of types makrobenthos by 7 species, number of individuals makrobenthos ind/m2 by 327, the number of types of plankton by 12 species, and the number of individual plankton of 1,340 ind / l). In general, the relationship between physical factors, chemical, and biological post-dredging shipping lanes limestone around the same Cilacapmemilikikecenderungan Donan River bahwakondisi declining water quality at Station 2, followed by Station 3 and Station 1.
Identifikasi dan Karakterisasi Isolat 23 Ducc Serta Kemampuannya dalam Memproduksi Enzim Inulinase Wijanarka Wijanarka; K Endang; Hermin Hermin
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 26, No 3 (2009)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (181.736 KB) | DOI: 10.20884/1.mib.2009.26.3.159

Abstract

Inulinase has a capacity to convert inulin into fructose hence it could be used in high fructose syrup production. Inulinase is not readily available in Indonesian market. This enzyme could be obtained from thermotolerant yeast found in Dahlia variabilis. Therefore this study was conducted to identify inulinolytic yeast in D. variabilis. The results showed  that an inulinolytic yeast isolate 23 DUCC from D. variabilis tubers was found. The inulinolitic yeast produces inulinase (E.C.3.2.1.7). Based on the identification and determination, the isolate is known to be Kluyveromyces marxianus.  The best optimation condition for this yeast was as follows: concentration of carbon source (inulin) was 0.75%, pH was 5.5 and time of fermentation was 53 hours. In such condition K. marxianus  produced inulinase up to 0.6481 IU.
Adsorpsi Zn dan Dekolorisasi Limbah Batik Menggunakan Limbah Baglog Pleurotus ostreatus dengan Sistem Inkubasi dan Volume Limbah Batik Berbeda Tjan Herwinda Kartikasari; Sri Lestari; Ratna Stia Dewi
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 29, No 3 (2012)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2012.29.3.252

Abstract

Batik industry is one of the traditional textile industry in Indonesia which produce wastewater. Pollutants that contained in wastewater such as heavy metal and dyes. White rot fungi (Pleurotus ostreatus) from spent mushroom known can adsorb heavy metal and can decolorize dyes in wastewater. Spent mushroom have potential as an adsorbent because it still contains mycelium and cellulose. The purpose of this research is to know the difference of incubation system and volume of waste batik for  adsorption Zn and decolorization by spent mushroom of P. ostreatus and the most optimal treatment adsorb Zn and decolorize waste batik. This research used experimental methods in Completely Randomized Design (CRD) with a split plot design. The main parameters observed were the value is the level of metal Zn and absorbance of waste batik. Data were analyzed by analysis of variance (F test) then proceed with a test of Honestly Significant Difference with an error rate of 1% and 5%. The result is spent mushroom of P. ostreatus able to adsorb Zn and to decolorize waste batik. The highest Zn adsorption is J2S treatment (62,406%) that was contained 75 ml indigosol green at static incubation. The highest decolorization is J1S treatment (77,991%) that was contained 50 ml indigosol green at static incubation.
Ekspresi Relatif mRNA BRLF1 Epstein-Barr Virus dari Biopsi Jaringan Tumor dalam Blok Parafin sebagai Petanda Biologi Patogenesis Karsinoma Nasofaring Andreanne Poppy Estania; Hidayat Sulistyo; Daniel Joko Wahyono
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 34, No 2 (2017)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2017.34.2.409

Abstract

Karsinoma nasofaring (KNF) merupakan tumor ganas pada sel epitel nasofaring dan merupakan penyakit multifaktor yang bersifat endemik. Tipe KNF tidak berdiferensiasi (KNF WHO-3) telah terbukti konsisten dengan infeksi EBV. Gen litik BRLF1 berperan sebagai mediator transisi dari fase laten menjadi litik. Pengukuran aktivitas mRNA EBV di lokasi tumor primer perlu dilakukan karena lebih mencerminkan patogenesis KNF yang  sesungguhnya dari pada diagnosis serologi dan pengukuran DNA EBV di sirkulasi darah. Penelitian ini bertujuan untuk mengetahui nilai ekspresi relatif mRNA BRLF1 Epstein-Barr Virus pada beberapa tingkat stadium tumor penderita KNF sebagai  petanda biologi potensial dalam patogenesis KNF. Metode penelitian yang digunakan adalah metode deskriptif yang berbentuk studi seran lintang. Sampel penelitian ini adalah biopsi jaringan tumor dalam blok parafin penderita KNF sebanyak 24 sampel. Sampel tersebut telah didiagnosis pasti sebagai KNF WHO-3 dari hasil pemeriksaan Patologi Anatomi pada Poli Patologi Anatomi, RSUD Prof. Dr. Margono Soekarjo, Purwokerto serta memenuhi kriteria inklusi sampel. Analisis statistik dilakukan untuk membandingkan ekspresi relatif mRNA BRLF1 stadium yang berbeda. Nilai ekspresi relatif mRNA BRLF1 EBV pada 24 sampel KNF berkisar 99,04415959-1097,496026. Hasil analisis statistik menunjukkan nilai ekspresi relatif mRNA BRLF1 EBV pada stadium awal (n = 5; 544,72420 + 142,614733) lebih tinggi 1,8 kali dibandingkan dengan stadium lanjut (n = 17; 395,68612 + 293,172201), namun peningkatan  ekspresi relatif mRNA BRLF1 EBV tidak bermakna secara statistik karena didapatkan nilai p=0,130 (p>0,05). Oleh karena itu, ekspresi relatif mRNA BRLF1 EBV dari biopsi jaringan tumor dalam blok parafin tidak berpotensi sebagai petanda biologi molekul patogenesis KNF, khususnya progresivitas tumor pada stadium lanjut KNF.
Kajian Etnobotani Mayarakat Adat Kampung Pulo di Kabupaten Garut Nurlina Ramdianti; Hexa Apriliana Hidayah; Yayu Widiawati
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 30, No 1 (2013)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2013.30.1.125

Abstract

Ethnobotany is important in Indonesia because there are a lot of unknown things about the traditional plant usages by traditional people. One of them is the Kampung Pulo’s traditional people in Garut District of West Java’s province. This research aims to determine the diversity, usage and part of plants used by Kampung Pulo’s traditional people. The methods used are survey and data collection has been exploration, by interview and direct observation. The data were by analyzed by using descriptive analysis  plant diversity, usage for, part used and how they were used by Kampung Pulo’s traditional people. The result of the research showed that there were 93 plants species included into 42 families which is used to Kampung Pulo’s traditional people. The most species used includes Areca catechu L., Arenga pinnata (Wurmb) Merr., Cocos nucifera L., Carica papaya L., Sauropus androgynus (L.) Merr., Gigantochloa verticillata (Willd) Munro, Oryza sativa L., Curcuma domestica L., Kaempferia galanga L. and Zingiber Officinale Roscc.. Kampung Pulo’s traditional people use the plants in a wide variety way such as for foods, shelter, tools, drugs, cosmetics, rituals, dye and traditional ceremonies. Plant parts that often use continously are fruits, leaves, stems, flowers, tubers, saffrons and roots.
Karakter dan Potensi Inokulum Bakteri Fosfat dalam Melepaskan Fosfor Tamad Tamad
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 31, No 2 (2014)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2014.31.2.212

Abstract

Soil bacteria group which can release soil P-adsorbed is phosphate bacteria (PB). PB can release soil P-adsorbed through: 1) acidification, 2) chelating 3) ligand exchange 4) mineralization and 5) compete colloid sorption site. PB have a potential to release soil P-adsorbed. This study was aimed to determining the character and PB inoculant potential in soil P-adsorbed release. The results of BLASTn PB showed that  isolate 1 was Pseudomonas trivialis, isolate 5 was Pseudomonas putida and  isolate 9 was Pseudomonas fluorescens. Based on the growth curve on day 5th (the end log. phase) population of Pseudomonas trivialis was 1010 CFU/mL, Pseudomonas putida was 1014 CFU/mL and Pseudomonas fluorescens was 1017 CFU/mL. Storage inoculant PB population decrease 97-84 % and 80-65 % PB lowering capabilities.
Aktivitas Enzim Peroksidase dan Kadar Klorofil pada Daun Angsana (Pterocarpus indicus) sebagai Peneduh Jalan yang terpapar Timbal Anjar Astuti Ferdhiani; Sri Lestari; Elly Proklamasiningsih
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 32, No 2 (2015)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2015.32.2.304

Abstract

One of emissions in the air that’s harmful to human  health is lead ( Pb ). The negative effects caused  by lead  and then required solutions for reducing the metal, its called phytoremediation. The Plant which acts as an agent of phytoremediation is Angsana (Pterocarpus indicus Willd.). Research to find out the lead content in Angsana’s leaf at four locations, knowing the influence of exposure to lead against the activity of an enzyme peroxydases and chlorophyll content in the Angsana’s leaf. The location for collection of samples conducted in four  location at Purwokerto (1) H.R Bunyamin, (2) Ovis Isdiman, (3) Gerilya, and (4) Dr. Soeharso. A method of the sample used purposive sampling.  Sample were analysed  using wet digestion method, then were analysed using Atomic Absorbance Spectrofotometer (AAS). The activity of peroxydases and levels of chlorophyll were analysed using spectrophotometer Uv Vis. Result showed that the lead content in Angsana’s leaf  is  0,1 – 0, 25 mg/L, the activity of peroxidases is  600- 1250 unit/g  and  chlorophyll content  is 9,0- 21,0 mg/L. The conclusion was no relations about chlorophyll content with levels of  lead content and the relations about activity of enzymes peroxydases and lead content is low . Content of  lead on Angsana’s leaf of  research has been done do not reach 1000 mg/L.

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