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Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal
ISSN : 08531629     EISSN : 08531629     DOI : -
Majalah Ilmiah Biologi Biosfera : A Scientific Journal merupakan peer reviewed jurnal yang diterbitkan oleh Fakultas Biologi Universitas Jenderal Soedirman. Media ini mewadahi hasil-hasil penelitian di bidang biologi tropika yang terbit tiga kali setahun (Januari, Mei, September).
Arjuna Subject : -
Articles 342 Documents
Diversitas Species Tumbuhan Paku Hias dalam Upaya Melestarikan Sumberdaya Hayati Kebun Raya Baturraden Sukarsa Sukarsa; Hexa Apriliana Hidayah; Titi Chasanah
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 28, No 1 (2011)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2011.28.1.257

Abstract

Baturraden Botanical Garden is a complex ecosystem. Its natural resources, including several species of fern, provide an opportunity to be managed sustainably so it can be used to fulfill a long term of public need. This research was aimed to know the diversity of fern species and its potential as ornamental plants. The research was using survey method. The data was analyzed using descriptive comparative analysis. Species identification was performed by comparing the samples with herbarium specimens and also using identification keys. During the observation, 26 species of fern were found, which is consisted of 14 terrestrial species and 12 epiphyte species. Nephrolepis bisserata (Sw.) Schott. was terrestrial species with highest individual number and widest dispersion, whereas terrestrial species with smallest individual number and the narrowest distribution was Blechnum orientale Linn. Epiphytic fern species with highest individual number and widest dispersion was Nephrolepis falcata (Cap.) C. Chr., whereas epiphytic species with smallest individual number and narrowest distribution was Davallia triphylla Hk. From 26 fern species which were found at Baturraden Botanical Garden, 22 fern species were potential as ornamental plant which can be grew up either on land, pot or on host plant.
Deteksi Gen Laten Membrane Protein-1 Epstein-Barr Virus Pada Karsinoma Nasofaring Uli Nurjanah; P Maria Hendrati; Anton Budi Dharmawan; Nur Signa A. Gumilas
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 33, No 2 (2016)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2016.33.2.466

Abstract

Penelitian ini bertujuan untuk mengidentifikasi gen LMP-1 EBV sebagai biomarka diagnosis KNF. Desain penelitian ini adalah studi cross-sectional dengan teknik consecutive sampling. Sampel adalah darah total pasien KNF WHO-3 yang dikumpulkan dari pasien yang belum menjalani terapi dari tahun 2014 pada Departemen Telinga Hidung Tenggorok - Kepala Leher, Rumah Sakit Prof. dr. Margono Soekarjo, Purwokerto. Total subyek penelitian adalah 22 orang untuk NPC WHO-3 pasien dengan informed consent. Sampel diisolasi dengan protokol kit Purelink® DNA / RNA (Invitrogen) untuk mendapatkan larutan DNA 100μL dan disimpan dalam waktu lama pada suhu -80 ° C. Teknik PCR konvensional dilakukan untuk mendeteksi gen LMP1 dengan mengamplifikaasi DNA gen EBV LMP1 yang menghasilkan amplikon DNA berukuran 142 bp. Analisis sensitivitas metode PCR dan hasil identifikasi gen LMP-1 menggunakan metode deskriptif dengan membandingkan hasil pendeteksian yang diperoleh pada penelitian dengan hasil penelitian terdahulu yang terkait. Hasil penelitian menunjukkan bahwa gen LMP-1 EBV sebagai biomarka pada diagnosis KNF dapat dideteksi dengan menggunakan teknik PCR konvensional yang menghasilkan amplikon DNA berukuran 142 bp. Sensitivitas gen LMP1 adalah 77,27%, menunjukkan sensitivitas tinggi. Sensitivitas PCR konvensional dalam mendeteksi gen EBMP LMP-1 menunjukkan sensitivitas yang lebih tinggi dibandingkan dengan penghapusan gen EBMP LMP-1 sebesar 30 bp dan LMP-2A EBV
Dekolorisasi Limbah Cair Batik Menggunakan Miselium Jamur yang Diisolasi dari Limbah Baglog Pleurotus ostreatus Anna Yulita; Sri Lestari; Ratna Stia Dewi
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 30, No 2 (2013)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2013.30.2.132

Abstract

Batik industries have negative impacts on the environment from the dye used. An alternative method for decolorization in batik wastewater using the fungi Pleurotus ostreatus was grown in baglog. Spent mushroom (baglog) still contains fungal mycelium of P. ostreatus and contaminant fungus such as Penicillium sp., and Aspergillus sp.). Those fungi have a potential to decoloring dyes in batik wastewater. The aim of this research was to determine the ability of fungal mycelium isolated from spent mushroom P. ostreatus at decoloring batik wastewater, and determine the most effective fungal mycelium to decoloring batik wastewater. The results showed that the mycelium isolated from sewage baglog P. ostreatus was able to decoloring batik wastewater. Penicillium sp. mycelium is most effective at decoloring batik wastewater.
Adaptasi Anatomis Tanaman Kedelai Varietas Slamet Akibat Perbedaan Ketinggian Tempat Juwarno Juwarno; Muachiroh Abbas; Eddy Tri Sucianto
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 31, No 1 (2014)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2014.31.1.218

Abstract

A research on the “Anatomical Adaptation of Soybean ‘Slamet’ in Various Altitudes” has been conducted. The research was aimed to traced anatomical adaptation of soybean ‘Slamet’ in various altitude and to proved altitude give the anatomical adaptation to soybean ‘Slamet’. The result of this research showed that altitude has caused the increase of leaf mesophyll thickness. The different altitude does not affect the cuticle thickness, length and width of stomata, stomatal and trichomatal density per mm2 of leaf area. The altitude affected the anatomical adaptation of soybean ‘Slamet’ was 250 m above sea level with the average mesophyll thickness of 112.40 µm. A research entitled on the “Anatomical Adaptation of Soybean ‘Slamet’ Variety Plant Because of in Various Altitudes Differentiation” have has been conducted. The research was aimed was to traced anatomical adaptation of soybean ‘Slamet’ variety plant because of in various altitude differentiation and to proved altitude differentiation give the anatomical adaptation of to soybean Slamet variety plant. The result of the this research showed that altitude differentiation has caused the increase of leaf mesophyll thickness raise of soybean Slamet variety leaf. The different altitude differentiation does not caused of affect the cuticle thickness, the length and width stomata, the stomatal and trichomatal density per mm2 leaf area. The altitude most affected on the anatomical adaptation of soybean ;Slamet; variety was 250 m above sea level with the most average mesophyll thickness of 112.40 µm.A research on the “Anatomical Adaptation of Soybean ‘Slamet’ in Various Altitudes” has been conducted. The research was aimed to traced anatomical adaptation of soybean ‘Slamet’ in various altitude and to proved altitude give the anatomical adaptation to soybean ‘Slamet’. The result of this research showed that altitude has caused the increase of leaf mesophyll thickness. The different altitude does not affect the cuticle thickness, length and width of stomata, stomatal and trichomatal density per mm2of leaf area. The altitude affected the anatomical adaptation of soybean ‘Slamet’ was 250 m above sea level with the average mesophyll thickness of 112.40 µm. A research entitled on the “Anatomical Adaptation of Soybean ‘Slamet’ Variety Plant Because of in Various Altitudes Differentiation” have has been conducted. The research was aimed was to traced anatomical adaptation of soybean ‘Slamet’ variety plant because of in various altitude differentiation and to proved altitude differentiation give the anatomical adaptation of to soybean Slamet variety plant. The result of the this research showed that altitude differentiationhas caused the increase of leaf mesophyll thickness raise of soybean Slamet variety leaf. The different altitude differentiation does not caused of affect the cuticle thickness, the length and width stomata, the stomatal and trichomatal density per mm2 leaf area. The altitude most affected on the anatomical adaptation of soybean ;Slamet;varietywas 250 m above sea level with the most average mesophyll thickness of 112.40 µm.
Keragaman dan Produktivitas Hijauan Pakan Indigenous pada Berbagai Tingkat Kerapatan Vegetasi di Pegunungan Kapur Gombong Selatan Doso Sarwanto; Sari Eko Tuswati; Pudji Widodo
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 32, No 3 (2015)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2015.32.3.337

Abstract

Research aims to conduct diversity and productivity of indigenous forage in different levels vegetation density in Gombong-Selatan karst mountain. We used survey method to explain the research by sampled three location of vegetation density, they are moderate, high, and very high. In each location vegetation density we take five parts by using random sampling. Then, a descriptive technique is used to analyzed the indigenous forage diversity data result whereas variance analysis is used to analyzed the productivity.The results shows the highest diversity of indigenous forage in Gombong-Selatan karst mountain is in moderate vegetation density area where has 36 species, high vegetation density has 20 species, and the highest vegetation density has 16 species. Eragrotis amabillis, Oplismenus burmannii, Centrosema pubescens, Ageratum conyzoides and Urena lobata are the dominant indegenous forage species in Gombong-Selatan karst mountain. The variances result shows that indigenous forage productivity in Gombong Selatan karst mountain is influenced by vegetation density. We found that lowest productivity is in high vegetation density.
Peanut Mottle Virus (Ptmtv) Contamination on Peanut Seeds Collected from Several Locations and Its Elimination by Hot Water Treatment Pudji Sulaksono
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 24, No 2 (2007)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2007.24.2.155

Abstract

The following observations on the seemingly prevalent occurrence of peanut (Arachis hypogaea) seed contaminations by PtMtV virus in the surrounding area of Palu, Central Sulawesi were conducted to determine the level of seed contamination and whether hot water treatment can be used to eliminate PtMtV virus from the seeds. Peanut seeds were collected from a number of locations and after soaking them in unheated water (29C) and heated water (55, 60 and 65C) for 10 minutes they were allowed to germinate, then transplanted and grown in pots in screen house. The effects of hot treatments on seed germinability, leaf formation, frequency of infection on surviving plants, and plant biomass production were determined. Seeds soaked in heated water showed different germinability, grew to become plants with different leaf formation and plant biomass production. Heat treatments gave different frequencies of infection on the plants. However, not a single heat treatment gave satisfactorily results in terms of giving zero or low infection and at the same time giving desirable seed germinability, leaf number, and plant biomass. The prospect of using hot water treatment at 55C or lower with longer soaking duration as a method for PtMtV virus elimination from peanut seeds is discussed.
Populasi Tungau Parasit Varroa jacobsoni: Penyebab Kematian Pupa dan Korelasinya terhadap Produksi Madu Apis cerana pada Sisiran Sarang Membujur dan Melintang Hery Pratiknyo; Darsono Darsono
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 29, No 3 (2012)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2012.29.3.248

Abstract

Bee keeping of local bee (Apis cerana Fab.) in Purbalingga Regency is spread widely from  25 m to 350 m asl, eventhough quatatively the production is still low. It is caused by parasitic mite attack,  Varroa jacobsoni. This mite attack then suck haemolymph of the pupae,  meanwhile if the bee comb cell  consist of more than one mites, the pupae will die, consequently the honey production will decrease. The aims of the research are: 1) to know a mount of individual mite living and caused the death of one bee pupae, .2) to define the correlationship between density of parasitic mite and honey production, 3). To differ parasite mite V. jacobsoni  density in longitudinal and cross posision of combs.  Method used was survey with purposive random sampling tehnique on two posision of bee comb, 15 samples were taken in each posision.  The data were analyzed  by counting the amount of mite living on every dead pupae and the correlation was analyzed for the relationship between relative density of parasite mite and  honey production. The result shows that correlation between relative density of parasitic mite and honey production is unsignificant. Conclusion of the research are: 1) amount of  parasitic mite living on bee pupae and causing death is 5,80 individual on cross posisition comb and 4,71 individual on longitudinal posision comb. 2) correlation between relative density of parasitic mite and honey production is unsignificant. 3). The relative density of parasitic mite between two posisions of comb is unsignificant.
Perkembangan Awal Protocorm Anggrek Phalaenopsis amabilis secara In Vitro setelah Penambahan Zat Pengatur Tumbuh α-Naphtaleneacetic Acid dan Thidiazuron Eka Fitriana Candra Ningrum; Ikhsanudin Nur Rosyidi; Rizka Riliant Puspasari; Endang Semiarti
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 34, No 1 (2017)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2017.34.1.393

Abstract

Phalaenopsis amabilis (L.) Blume merupakan salah satu anggrek alam dengan nilai komersial yang tinggi. Keberadaannya di alam semakin sukar dijumpai akibat eksploitasi berlebihan dan kerusakan hutan. Selain itu budidaya anggrek ini cukup sulit dilakukan karena biji bersifat mikroskopis dan tidak memiliki endosperm, sehingga perlu dilakukan kultur  in vitro. Dalam budidaya secara in vitro diperlukan medium pendukung pertumbuhan anggrek secara optimal misalnya dengan penambahan Zat Pengatur Tumbuh (ZPT) dari golongan auksin dan sitokinin. Penelitian ini bertujuan untuk mengetahui pengaruh penambahan ZPT tersebut terhadap perkembangan protokorm (pertumbuhan embrio anggrek) P. amabilis secara in vitro dari aspek morfologi dan anatomi. Penelitian dilakukan di Laboratorium Bioteknologi dan Laboratorium Struktur Perkembangan Tumbuhan. Digunakan protokorm anggrek P. amabilis berumur 2 bulan. Protokorm disubkultur pada medium NP dengan kombinasi ZPT NAA dan TDZ pada berbagai konsentrasi dan diinkubasi pada ruangan dengan kelembaban tinggi, suhu 250C.  Kemudian diamati protokorm anggrek selama 2 minggu yang mencakup persentase pertumbuhan, persentase protokorm yang membentuk tunas dan absorbing hair. Pembuatan preparat anatomi dilakukan dengan mengambil protokorm setiap minggunya, disimpan dalam larutan alkohol 70% dan dibuat preparat anatomi menggunakan metode Parafin dengan pewarnaan safranin. Hasil penelitian menunjukkan pada penambahan NAA dan TDZ pertumbuhan protokorm mencapai 100%. Persentase protokorm yang membentuk tunas berturut turut (0:0, 0.2:0, 0:0.5, 0.2:0.5, 0:1, dan 0.2:1) ppm adalah 24%, 22%, 32%, 40%, 40% dan 38% sedangkan untuk pengamatan protokorm yang membentuk absorbing hair sebesar 10%, 18%, 14%, 24%, 16%, dan 0%. Untuk diameter sel berukuran 39.2±0.47µm, 44.4±0.97µm, 39.08±0.5µm, 38.83±0.2µm,39.5±0.39µm dan 39.75±0.28µm. Sehingga dapat disimpulkan medium yang paling optimal dalam pertumbuhan anggrek P. amabilis secara in vitro adalah medium NP dengan kombinasi NAA dan TDZ (0.2 : 0.5) ppm.Kata kunci : Anatomi, Kultur In Vitro, Morfologi, Phalaenopsis amabilis
Status Kerentanan Aedes aegypti (Diptera: Culicidae) pada Tahun 2006-2007 terhadap Malation di Bandung, Jakarta, Surabaya, Palembang dan Palu Intan Ahmad; Sita Astari; Resti Rahayu; Nova Hariani
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 26, No 2 (2009)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2009.26.2.119

Abstract

Distribusi Phyllanthus emblica L. di Sumatera Utara Bagian Selatan Ummi Khoiriyah; Nursahara Pasaribu; Saleha Hannum
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 32, No 2 (2015)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2015.32.2.300

Abstract

The Indian gooseberry (Phyllanthus emblica L.) distribution has been studied in the southern part of North Sumatera, which is in the South Labuhanbatu district, North Padanglawas, Padanglawas, SouthTapanuli, Padangsidempuan city and Mandailing Natal district. The aims ofthis study was to determine the distribution area of balakka based on rainfall, lands cover and soil type. The data were collectedby observation. Distribution data are presented in the  form of map using ArcView 3.3 Software. The results of the analysis showed that the common balakka was distributed in the rainfall region of 2000 – 2500 mm/year in dryland farming and the humic acrisols soil.

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