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Jurnal Perikanan Universitas Gadjah Mada

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Published by Universitas Gadjah Mada

ISSN : 08536384 EISSN : 25025066 DOI : -

Core Subject : Science, Agriculture,

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology

Jurnal Perikanan Universitas Gadjah Mada are published to promote a critical review of the various investigative issues of interest in the field of fisheries between the researchers, academics, students and the general public, as a medium for communication, dissemination, and utilization of wider scientific activities.

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Articles 460 Documents

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Penggunaan Gen GH sebagai Marka Molekuler DNA Gurami, Osphronemus goramy dalam Pengembangan Teknologi Surrogate Broodstock Achmad, Marlina; Alimuddin, Alimuddin; Carman, Odang; Arfah, Harton; Zairin, Muhammad
Jurnal Perikanan Universitas Gadjah Mada Vol 11, No 2 (2009)
Publisher : Universitas Gadjah Mada

The technology of fish germ cell transplantation had been established to create broodstock systems by which a target offspring can be produced from a surrogate parent. Donor cell for transplantation is derived from transgenic fish carrying green fluorescent protein gene functions as a marker to distinguish the donor from recipient cell. In this study, an alternative technique was developed for identifying gouramy-derived donor cell and Nile tilapia as recipient by PCR amplification method using growth hormone (GH) gene as a molecular marker. Specific primer for GH gouramy was designed by using Genetyx version 7 software. ß-actin gene was used as an internal control of DNA loading. The result showed that a specific PCR amplification product of 340 in length was obtained when the DNA template was from gouramy, while no PCR product from Nile tilapia. The minimum concentration of genomic DNA of gouramy mixed with a 700 ng/µl of Nile tilapia that could be detected by PCR was 1 ng/µl. Thus, PCR method with specific GH primer may be useful to detect the incorporation of donor cell in recipient gonad towards development of surrogate broodstock technology.

Biodiversity Different and Species Assemblages of Phytoplankton Within Ooze Sediment at Myll Lakes, New South Wales Australia Rukminasari, Nita
Jurnal Perikanan Universitas Gadjah Mada Vol 11, No 2 (2009)
Publisher : Universitas Gadjah Mada

Phytoplanktons are primary producer, which determine the waters productivity. The assemblages of phytoplankton in the lake varied spatially and temporally as a result of nutrient concentration, some physical and chemical factors. However, the assemblage of phytoplankton in the lake was not well documented. The overall aim of the study was to count and identify phytoplankton taxa and to examine assemblages at location with and without ooze, and to relate the presence/absence of ooze to phytoplankton abundance and assemblages. The samples were collected from Myall Lakes, NSW Australia. The data were analysed using multivariate analysis to determine assemblages of phytoplankton amongst location. Furthermore, univariate analysis was used to examine how the water quality was in particular presence/absence of ooze affected to phytoplankton abundance. The hypothesis being tested was: (1) phytoplankton assemblages vary spatially in Myall Lake due to varying water quality characteristics, including presence of ooze, (2) there was a significant difference of phytoplankton abundances between locations due to presence/absence of ooze.In both instance the hypothesis on this study was accepted. There was a significant different not only phytoplankton assemblages but also phytoplankton abundance within locations. It concluded that the phytoplankton varied spatially in those locations. Presence/absence of ooze affects abundance of phytoplankton. However, further study was needed to conduct in particular to determine certain aspects which affect the assemblages and abundances of phytoplankton.

Population of Esteochilus spp. as a Base of Sustainable Fishing in Serayu River Lestari, Windiarini; Putranto, Yogi; Setyaningrum, Nuning; Sugiharto, Sugiharto
Jurnal Perikanan Universitas Gadjah Mada Vol 11, No 2 (2009)
Publisher : Universitas Gadjah Mada

The increasing demand for freshwater resources generated by human population growth, urbanization, industrialization and irrigation will result in a decline or loss of freshwater fish such as Osteochilus spp. The aims of research were to analysis the population of Osteochilus spp in terms of abundance, longitudinal distribution and sex ratio and to determine environmental factors of river driven these populations. The research was carried out in upper part of Serayu River during wet season (January – March) and dry season (June - August) 2007. Fish was collected by using electro fishing and stratified random sampling was applied by dividing this river into five strata based on altitude. The result showed that the total abundances of Osteochilus spp. was 149 individuals, it was composed by of O. hasselti (47) with 8.30 – 24.35 cm and 11.20 – 198.70 g, O. microchephalus (101) with 11.35 – 25.21 cm and 17.9 – 206.5 g and O. kahajanensis (1) with 17.30 cm and 68.80 g. The size of population O hasselti and O. microchephalus gradually increased from 7 and 4 individuals in stratum A became 27 and 12 individuals in stratum D, and then increased up to 43 and 17 individuals in stratum E. The sex ratio of O. hasselti was 10:16 and O. microchephalus was 20:23. Both species were longitudinally distributed in Serayu River. It seems that the population of O. hasellti and O. microchephalus were more influenced by physical factors i.e. depth and current rather than chemical factors i.e. DO and pH of Serayu River.

Pengaruh Salinitas terhadap Perkembangan Parasit pada Benih Gurami, Osphronemus goramy Rahayu, Nani S.; Susanti, Dewi; Lantiani, Dwi; Wibowo, Sutopo A.; Diana, Roosita; Murwantoko, Murwantoko
Jurnal Perikanan Universitas Gadjah Mada Vol 11, No 2 (2009)
Publisher : Universitas Gadjah Mada

Research was conducted to know the effect of salinity on the reduction of parasites in Gouramy (Osphronemus goramy). The Research was performed using completely Randomized design with 5 salinity treatments (water pond, water with salt concentration 0, 2, 4 dan 6 g/L) with 4 replications. The 5-7 cm of gouramy fingerling were reared as on treatments and the parasites were counted using skin scrapping dan wet mount methods. Observation was performed every 3 day for 15 days on parasite intensity, prevalence, mortality, symptoms and water quality.The result showed that several parasites including Trichodina sp., Apiosoma sp., Ichthyophthirius sp., Oodinium sp., dan Henneguya sp were observed. The highest intensity and prevalence was found in Trichodina sp. The Salinity and lenght administration were significantly decrease number parasites. Salinity treatments (2, 4 dan 6 g/L ) for 3 days could totally removing Trichodina sp. from fishes. Meanwhile the data of other parasite species were unsignificant due to the low level of its intensity and prevalence. Salinity administration in level 6 g/L could reduce mortality.

Identifikasi Cacing Polychaeta, Nereis sp. sebagai Vektor White Spot Syndrome Virus (WSSV) di Alam dan Kajian Uji Tantangnya di Laboratorium Bambang W. Prastowo; Kade Ariawan; Evy M. Nur; Rahayu Rahardianti; Yuni Setyowati
Jurnal Perikanan Universitas Gadjah Mada Vol 11, No 2 (2009)
Publisher : Universitas Gadjah Mada

Polychaete worm is an important zoobenthos at shrimp culture system, however since there is no any culture of these worm in Indonesia, then all the worm biomass collected from their natural habitat. It raised some concern about their safety because polychaete worm might be get infected and will realistic as a pathway for virus to attack shrimp broodstock in the hatchery. Fresh polychaete worms (1 g wet) used as a test organisms were caught from pond area in Semat region, Jepara. The step of these experiment are identification of WSSV infection at polychaete worms in wild with PCR analysis, visual, microscope and histology observation. Followed by challenge test of polychaete worm with WSSV, and WSSV infectivity study at black tiger shrimp broodstock. It seen, from the experiment, that using two different PCR methods, OIE (2006) and Nugen kit, and also histology analysis showing no WSSV infection in polychaete worms. However, from further experiment showing that polychaete worm challenge by WSSV have already got severely stress due to WSSV inocculum treatment, but the level of infection is still very low so that can not detect by PCR. Shrimp broodstock that have already fed with WSSV-contaminated polychaete worms for 1 week, based on clinical signs seems to get infected by WSSV, even the infectivity level is still very low. It concluded that polychaete worm can be a vector for WSSV in pond.

Efektivitas Kanamycin terhadap Furunculosis pada Karper, Cyprinus carpio Sri Retnoningsih; Kamiso H. Nitimulyo; Kardiman Lanadimulya; Suprayogi Suprayogi; Supardi Supardi; Djoko Darmantani; I P. Panca; Hasnah Hasnah; Soefaad Soefaad; Milis Milis
Jurnal Perikanan Universitas Gadjah Mada Vol 11, No 2 (2009)
Publisher : Universitas Gadjah Mada

The aim of experiment was to know the appropriate and effective dosis of kanamycin antibiotic in order to recover and clean the Aeromonas salmonicida which caused furunculosis in carp, Cyprinus carpio. The experiment consisted of the preliminary test [revirulence of A. salmonicida, sensitivity, minimum inhibitory concentration (MIC), minimum bactericidal concentration test (MBC), lethal concentration-50 (LC50) of A. salmonicida to carp] and the effectiveness antibiotic kanamycin test. The water quality was analyzed at the beginning and the end of the experiment, histopathology was analyzed at the end of the experiment. The data were analyzed using analysis of variance (ANOVA) continued by least significant different and regression tests.Based on the result of in vitro test, it was obtained that the value of MIC was 4 ppm and the value of MBC was 8 ppm, while the result of lethal concentration-50 (LC50) A. salmonicida to carp was 1.07 x 105 cell/ml. Survival rate of carp at the end of experiment were 20% (treatment 0 ppm); 73,3% (treatment 16 ppm); 86.7% (treatment 32 ppm); 93.3% (treatment 48 ppm); 96.7% (treatment 64 ppm); and 100% (treatment 80 ppm). The survival rates were very significance difference (P <0,01) among treatments and the treatments of kanamycin were very significant difference (P <0.01) from positif control. The regression showed that there was correlation between the concentration of kanamycin and survival rates of carp by coefficient of correlation (R2) at 0.9382.

Peringatan Dini Pencemaran Logam Berat dan Pestisida Berdasarkan Rasio Seks Anakan Daphnia sp. Ahmad S. Mubarak; Juni Triastuti
Jurnal Perikanan Universitas Gadjah Mada Vol 11, No 2 (2009)
Publisher : Universitas Gadjah Mada

Pesticides and heavy metal are the major contributor for environmental pollution and have been spread out widely in aquatic environments. Daphnia sp. is a zooplankton that mostly used to detect aquatic pollution. The aim of this research was to study the effect of exposuring different concentration of heavy metal (Pb and Cd) and pesticides (diazionin) to the sex ratio offspring of Daphnia sp. The experiment composed of three kind of treatments (Cd, Pb and diazinon), each consist of 5 treatments namely 25%, 50%, 75%, 100% and 0% as control, and 4 replications. The data was analyzed with Kruskal Walace. If there was any different in each treatment, then was analyzed with Duncan Double Gap Test and linear regression. The research showed that exposuring Cd, Pb and diazinon with different concentration to the Daphnia sp. during 96 hours could increase the percentage male offspring of Daphnia sp. Concentration of Pb, Cd and peticides gave positive correlation to the sex ratio offspring of Daphnia sp. Further research was needed to know the practical technology using sex ratio offspring of Daphnia sp. to detect the level of pollution.

Pemeliharaan Ikan Beronang, Siganus gutatus sebagai Biokontrol Perkembangan Lumut, Chaetomorpha sp. dan Enteromorpha intestinalis di Tambak Suharyanto Suharyanto
Jurnal Perikanan Universitas Gadjah Mada Vol 11, No 2 (2009)
Publisher : Universitas Gadjah Mada

The aim of this research was to control the growth of green algae (Chaetomorpha sp and Enteromorpha intestinalis) by rabbit fish (Siganus gutatus) reared in brackishwater ponds in different stocking density. The research was conducted in the Research Station of Research Institute for Coastal Aquaculture, Marana, Maros South Sulawesi for 100 days. Nine brackishwater ponds in the size 100 m2 (3 ponds), 125 m2 (3 ponds) and 150 m2 (3 ponds) with the green algae growth were used respectively in this research. Group randomized design was used in this research. This research use three stocking density of rabbit fish in thriplicates that were (A) 20 ind/100m2 , (B) 30 ind/100 m2 (40 ind/125 m2), and (C) 40 ind/100m2 (60 ind/150 m2), respectively. The rabbit fish used in this research were 9.1+ 0.8 cm in length and 15+ 0,6 g in body weigth. The monitored variables were body length and weigth, survival rate and water quality. The result showed that the effect of stocking density not significantly different on the growth of body length and weight and survival rate of rabbit fish reared in brackishwater pond (P>0.05), but significantly different on the productivity of rabbit fish (P<0.05). Green algae growth in the brackishwater ponds could be controlled with the rearing of rabbit fish in the stocking density 20 - 40 ind/100 m2.

DAMPAK MINYAK BUAH MERAH, Pandanus conoideus LAM PADA PERFORMANSI YUWANA KERAPU SUNU, Plectropomus leopardus Titiek Aslianti; Philip Teguh Imanto; Made Suastika
Jurnal Perikanan Universitas Gadjah Mada Vol 11, No 1 (2009)
Publisher : Universitas Gadjah Mada

Market price of coral trout grouper was increasing by the color performance of fish. Beta-carotene diet was proposed to escalate pigmentation of coral trout grouper. Red fruit oil (Pandanus conoideus, Lam) which known as potential beta-carotene source was used in coral trout diets. The dosage of 5, 2.5 and 0 ml were supplemented to the every kilogram of pellet fed as a treatment in this observation during three-month period. Three hundred and fifty juveniles with initial total length 14.28±0.2 cm and 43.41±2.62 g of body weight were stocked in fiberglass tank of 5m³ seawater. Growth data were collected monthly, while color performance was taken by digital photography at the end of observation and analyzed by "Microsoft Adobe Photoshop 8". Carotene contents in the diets and fish body result showed that the Pandanus conoideus oil has role in increasing performance of coral trout with more bright color; five ml dosage gave the best growth performance with 35.57% and 185% for total length and body weight respectively, with 90% survival rate and 1.2 feed conversion ratios.

KASUS INFEKSI IRIDOVIRUS PADA BENIH IKAN KERAPU PASIR, Epinephelus corallicola DI HATCHERY Fris Johnny; Des Roza
Jurnal Perikanan Universitas Gadjah Mada Vol 11, No 1 (2009)
Publisher : Universitas Gadjah Mada

Seeding effort of coral grouper fish, Epinephelus corallicola in Research Institute for Mariculture (RIM) Gondol, Bali has been blazed the way, but rearing seed still found constraint as height of mortality. One of death cause of seed is by virus infection disease. An attempt for diagnosis iridovirus infection at seed coral grouper Fish age sand around 60 days shows symptom as no eating, populating in basin base, colour turn darken, and here in after death. Disease detection of the showing symptom seed that has not generated death is observed based on pathological anatomy and viral detection with PCR-probe. Disease detection also conducted to seed after showing symptom and generated high death. Detection based on pathological anatomy result many "giant cells" from spleen organ. Disease detection based on PCR-probe of seed which has not died yield thin positive band of iridovirus, while observation of death seed yields high crystal clear positive band of iridovirus at 570bp.

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