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Dr. Ir. Dyah Rini Indriyanti
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INDONESIA
Biosaintifika: Journal of Biology & Biology Education
ISSN : 2085191X     EISSN : 23387610     DOI : -
Core Subject : Agriculture,
Biosaintifika Journal of Biology & Biology Education, an electronic international journal, provides a forum for publishing the original research articles, review articles from contributors, and the novel technology news related to biological research and biological education.
Arjuna Subject : -
Articles 1,068 Documents
Frontmatter (Cover and Table of Contents) Biosaintifika Biosaintifika
Biosaintifika: Journal of Biology & Biology Education Vol 13, No 2 (2021): August 2021
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v13i2.33137

Abstract

Toxicity of Miana Leaf (Coleus blumei) Extract Against Houseflies (Musca domestica) Surahmaida Surahmaida; Umarudin Umarudin
Biosaintifika: Journal of Biology & Biology Education Vol 11, No 2 (2019): August 2019
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v11i2.19402

Abstract

Houseflies (Musca domestica) is an disease-carrying insect  that plays an important role in the spread of disease in humans and animals. One way to control houseflies is using Miana leaves (Coleus blumei) as botanical pesticide. The purpose of this study was to determine the chemical compounds contained in Miana leaf as well as determine the optimal concentration of Miana leaf extract in causing knockdown effect in houseflies. Miana leaf fine powder was soaked with methanol (maceration) for 3 days followed byphytochemical screening (chemical reagents and Gas Chromatography Mass Spectrophotometry (GCMS) and anti-houseflies toxicity tests. Testing of methanolic extract of Miana leaves was made in the form of spray using the knockdown method at concentrations of 5%, 10%, 20%, 30% and 40% and observed in 5, 10 and 15 minutes. The results of phytochemical screening with chemical reagents showed that the extract contained alkaloids, tannins, flavonoids, saponins and terpenoids; while the results of GCMS analysis showed 8 bioactive compounds. The results of the study revealed that at a concentration of 30% and 40%, methanolic extract of Miana leaves could result in a knockdown effect that was accompanied by death with a percentage value of 100% in the first 5 minutes of observation. This research shows that methanolic extract of Miana leaves can be used as an alternative to botanical pesticides in controlling houseflies (Musca domestica).
Problem-Based Learning Module of Environmental Changes to Enhance Students’ Creative Thinking Skill Pamula Guruh Prastiwa Anjarwati; Sajidan Sajidan; Baskoro Adi Prayitno
Biosaintifika: Journal of Biology & Biology Education Vol 10, No 2 (2018): August 2018
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v10i2.12598

Abstract

Creative thinking skills are important for long-term success. Empowering creative thinking skills which can be trained through problem-solving activities in learning, thereby promoting high cognitive engagement. This study aimed to test the effectiveness of the research and development product of Problem-Based Learning module of environmental changes in empowering students’ creative thinking skills. The research method used was the Quasi-experiment with nonequivalent control group design. The sample of the research was the tenth-graders of a Senior High School in Karanganyar district, Central Java. The sampling was cluster sampling by involving two classes, namely the Grade X-MIA 1 with 28 students and the X-MIA 4 with 28 students. The qualitative and quantitative-descriptive approaches were used to analyze the data. The result shows the mean of creative thinking skills score of experiment group is higher than the control group. This developed module could empower students’ creative thinking skills by problem-solving activities as needed for Indonesian educational goal . The study concludes that development module was constructed based on Problem-based Learning activities in the module could stimulate students solved and identified the problems with their ideas and developed their creative thinking.
KANDUNGAN BAHAN ORGANIK DAN AKUMULASI MINERAL TANAH PADA BANGUNAN SARANG RAYAP TANAH Macrotermes gilvus Hagen (BLATTODEA: TERMITIDAE) Niken Subekti
Biosaintifika: Journal of Biology & Biology Education Vol 4, No 1 (2012): March 2012
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v4i1.2263

Abstract

Rayap Macrotermes gilvus Hagen mempunyai peranan ekologis rayap tanah M. gilvus sebagai degradator primer di dalam hutan, eksplorasi perananannya sebagai agen biologis dalam perbaikan vegetasi dan perbaikan kualitas tanah. Rayap dapat memodifikasi sifat fisik dan kimia tanah. Penelitian tentang kandungan bahan organik telah dilakukan dengan analisis proksimat (metode Weende), sementara akumulasi mineral tanah menggunakan metode X-Ray berdasarkan Analysis Program Cristallynity. Rayap M. gilvus Hagen merupakan komponen penting dalam memodifikasi beragam mineral dari tanah disekitarnya. Hasil penelitian menunjukkan bahwa terdapat perbedaan nyata antara komposisi mineral tanah dalam sarang rayap M. gilvus Hagen dengan mineral tanah disekitar sarang. Hasil penelitian menunjukkan kandungan bahan organik dalam bangunan sarang menghasilkan sebesar 98.33% dan padatannya 1.67%. Padatan ini terdiri dari karbohidrat sebesar 3.16%, abu 4.19%, lemak 23.95%, protein sebesar 39.52%, dan sisanya 29.18% berupa mineral-mineral. Bangunan sarang rayap yaitu SiO2 dan Despujolsite yang dibawa dari lingkungan sekitar kedalan bangunan sarang. Unsur-unsur yang lain diperoleh dari sebagian material yang berasal dari saliva, humus dan tanah sekitar sarang. The termite Macrotermes gilvus Hagen plays an ecological role. Subterranean termites M. gilvus is considered as the primary degradator in the forest, and therefore the exploration of its role as the biological agent to recover the vegetation and soil quality might be useful. Termites could modify the physical and chemical nature of soil. M. gilvus Hagen was an important component in modifying various minerals of the surrounding soil.  Research on the content of the organic materials had been proximat analysis (Weende methode), and the accumulation of soil mineral structure in the mound with X-Ray Methode (Analysis Program Cristallynity 2006). The result of the research indicated that there was significant difference between the composition of soil minerals in the mound of M. gilvus Hagen and the soil minerals around the mound. Analysis of the organic material in the mound building showed that the water was 98.33% and the solidity level was 1.67%, this solidity consisted of carbohydrate as much as 3.16%, ash as much as 4.19%, fat as much as 23.95%, protein as much as 39.52% and other minerals as much as 29.18%. The mound building in the minerals namely, SiO2 and Despujolsite, seemed to be carried in from the surroundings into the mound building. The other elements were obtained partly from the saliva, the fertile soil and the soil around the mound.
Effect of Temperature Shock on the Triploidization Success of Seurukan Fish (Osteochilus vittatus) Kartini Eriani; Alfis Syahrin; Zainal Abidin Muchlisin
Biosaintifika: Journal of Biology & Biology Education Vol 9, No 2 (2017): August 2017
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v9i2.8680

Abstract

Seurukan fish (Osteochilus vittatus) has many advantages, besides the fish also has disadvantages which are the slow growth, so the temperature shock of triploidization technique was expected to solve the problem. The objective of the present study was to obtain an effective temperature to increase of triploidization success of seurukan fish (Osteochilus vittatus). The experimental method and completely randomized design model were used in this study. Five levels of temperature shocks at three replicates were tasted: 4℃ (cold), 6℃ (cold), 28℃ (normal), 35℃ (heat) and 37℃ (heat). The sperms and eggs were fertilized in the plastic jar then a total 100 of fertilized eggs (zygotes) were taken randomly 3 minutes after fertilization and soaked in respective temperature for 90 seconds, and then incubated in incubation jars at the water temperature of 28-29℃. The results showed that the temperature shock gave the significant effect on the hatching and the success of triploidization success (P˂0.05), but did not give the significant effect the fertility and survival rates (P0.05). The triploid fish can be achieved using cold and heat shock, but the higher triploid fish was recorded at 37℃ was the best temperature recommended for triploidization of Seurukan fish.
Exploration of Potential Actinomycetes from CIFOR Forest Origin as Antimicrobial, Antifungus, and Producing Extracellular Xylanase Sipriyadi Sipriyadi; Yulin Lestari; Aris Tri Wahyudi; Anja Meryandini; Maggy Thenawidjaja Suhartono
Biosaintifika: Journal of Biology & Biology Education Vol 8, No 1 (2016): March 2016
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v8i1.5052

Abstract

This study aimed to isolate and explore the actinomycetes of CIFOR forest origin as an antimicrobial and antifungal agent, to produce an extracellular xylanase, and to identify isolates based on 16S rRNA gene sequences. Actinomycetes were isolated using Humic-acid Vitamin-B agar (HV) media. Actinomycetes colonies that grow on the medium HV was subsequently purified by growing them on yeast malt agar (YMA) media), then an antagonistic test of selected bacteria against Bacillus sp., Escherichia coli, Fusarium oxysporum, and Sclerotium sp was performed. Xylanase activity test was detected by observing a clear zone, followed by identification. Total of 35 isolates of actinomycetes isolated based on their colony morphology characteristics and diverse types of spore chains showed Streptomyces spp. of isolates CFR-06, CFR-15, CFR-17, CFR-18, and CFR-19 were able to inhibit the growth of Bacillus sp.. The highest inhibition zone has a diameter of 10.1 mm (isolate CFR-17). Isolates CFR-01 and CFR-15 were able to inhibit the growth of E. coli with the highest inhibition zone diameter of 5.1 mm (isolate CFR-15). Isolates CFR-29 and CFR-12 were able to inhibit the growth of F. oxysporum while isolate CFR-35 were able to inhibit the growth of Sclerotium sp.. Xylanase activity test showed that isolates CFR-12, CFR-20, CFR-22, CFR-24, CFR-25, CFR-30, CFR-33, CFR-34 have an ability to produce extracellular xylanase enzyme. Actinomycetes isolate (Xyl_22) as a potential xylanase enzyme producer was closely related with Streptomyces drozdowicii by the maximum similarity of 99%.How to CiteSipriyadi, S., Lestari, Y., Wahyudi, A., Meryandini, A., Suhartono, M. T. (2016). Exploration Potential CIFOR Forest actinomycetes origin as Antimicrobial, Anti Fungus and Producing Enzymes Extracellular Xylanase. Biosaintifika: Journal of Biology Biology Education, 8(1), 94-102.
Plant Diversity and Composition of Three Different Altitudes in Sikka Forest Area - East Nusa Tenggara Abban Putri Fiqa; Gebby Agnessya Esa Oktavia
Biosaintifika: Journal of Biology & Biology Education Vol 12, No 2 (2020): August 2020
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v12i2.23565

Abstract

East Nusa Tenggara located in the Wallacea area that has many endemic species. However, the existence of germplasm diversity in this area has been threatened due to landscape changes and deforestation. The research was conducted to identify floristic composition in Sikka forest area. This research also aimed to compare that plant composition in Sikka forest area on each of the different altitudes, including their diversity richness and environmental factors. Two sampling methods were used, which are Point-Centered Quarter (PCQ) method that applied for trees and nested sampling method 5x5 m2 for sapling and 2x2 m2 for ground cover. Measured parameters were Relative Density (RD), Relative Frequency (RF), Relative Coverage/dominance (RC), Important Value Index (IVI), Diversity Index (H'), and Jaccard Similarity Index (J’). Research results showed that the diversity index of each region was relatively low. Plant composition of tree, sapling, and groundcover in three different altitudes was quite different. This result indicates that the plant composition in the Sikka, East Nusa Tenggara is very diverse. The temperature and relative humidity in each altitude area observed was significantly different. One of the endemic species of Flores Island, Eucalyptus urophylla, was only found at 500-700 m asl. There are very limited research on the vegetation diversity held in Flores, East Nusa Tenggara. This research can be the basic for further research in order to explore the potential diversity of flora in this area.
The Effectiveness of Process-Oriented Guided Inquiry Learning to Improve Students’ Analytical Thinking Skills on Excretory System Topic Ihwan Rosadi; Maridi Maridi; Widha Sunarno
Biosaintifika: Journal of Biology & Biology Education Vol 10, No 3 (2018): December 2018
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v10i3.15990

Abstract

Analytical thinking is a fundamental skill in education and which needs to be developed. This research would describe the effectiveness of process-oriented guided ınquiry learning (POGIL) to improve students’ analytical thinking skills on the topic of excretory system. This study used Research and Development (RD) design. It was conducted on the 11th grade students of senior high school in Ngawi, East Java in 2017/2018 academic year with one group pre-test and post-test design. The data collection techniques used were validation, observation, and test. The data were analyzed descriptively, whereby the analysis result was tested with the Gain test. The result showed that the students’ analytical thinking skills was significantly increased as stated by the N-gain score. In control class, there were 2 students (6.7%) whose N-gain score was in the low category, 25 students (83.3%) whose N-gain score was in the medium category, and 3 students (10%) whose N-gain score was in the high category. In the experimental class, there were 21 students (70%) who got N-gain score in the medium category, and 9 students (30%) who got N-gain score in the high category. The results of this research showed that the students analytical thinking skills were different after the implementation of POGIL method. Therefore, it could be concluded that the POGIL was effective to improve students’ analytical thinking skills on the excretory system in senior high school. The information obtained from this research can be used as a tool to improve students’ analytical thinking skills.
Production Improvement of M1 Generation Garut (Maranta arundinacea) Rhizomes through Gamma Radiation Mutation Puspita Deswina; Dody Priadi; Yashanti Berlinda Paradisa; Yuliana Galih Dyan Anggraheni; Enung Sri Mulyaningsih; Ambar Yuswi Perdani; Sri Indrayani
Biosaintifika: Journal of Biology & Biology Education Vol 13, No 3 (2021): December 2021
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v13i3.31383

Abstract

Garut (Maranta arundinacea L.) is a tuber containing flour with a low Glycemic Index (IG) suitable for diabetics and other degenerative diseases. It needs to be developed to reduce the dependence on imports or to substitute wheat flour. The objective of the study was to obtain superior arrowroot seedlings and observe the effect of gamma-ray irradiation on the density and position of stomata on arrowroot leaves. In this research, the irradiation of five arrowroot accessions of second-generation (MV1) with gamma rays (0, 10, 20, 30, 40, and 50 Gray) was carried out to obtain mutants with superior character so that they could be used as parent plants for arrowroot flour production. The treatments administered were arrowroot accessions (V), gamma-ray radiation dose (R), and their interaction. The qualitative and quantitative characters on plant characteristics, tuber production, and arrowroot leaves' stomata were observed. The research results showed that increased production is primarily for 25-Pandeglang accession (808.33 grams) and 10 Gray (800.00 grams) of gamma ray radiation treatment. It is expected that this accession can be released as a new variety candidate after subsequent selection and evaluation in a further generation. Moreover, the dose of gamma-ray radiation is inversely proportional to the number of stomata, which will increase the photosynthesis, thus increasing the number of tubers produced.
Antidiabetic Potential of Methanol Extract of Flamboyant (Delonix regia) Flowers Kartini Eriani; Uswatun Hasanah; Rizky Fitriana; Widya Sari; Yunita Yunita; Al Azhar
Biosaintifika: Journal of Biology & Biology Education Vol 13, No 2 (2021): August 2021
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v13i2.30080

Abstract

Providing scientific evidences for the medicinal benefits and possible toxic effects of the flamboyant flowers (Delonix regia (Bojer ex Hook.) Raf.) are very important to implementing the medicinal plant in this modern era. This study aimed to investigate antidiabetic potential of methanol extract of flamboyant flowers using a completely random design. Twenty-four male rats were randomly divided into 6 treatment groups with 4 replications: healthy rats (normal control, P1), diabetic rats treated with flamboyant flower extract of 0 (negative control, P2), 100 (P3), 200 (P4), and 400 mg/kg BW (P5), and rats which were induced by 0.45 mg/kg BW of Glibenclamide (positive control, P6). Diabetic condition was achieved by a single injection of alloxan 150 mg/kg BW. Treatments were given once a day for 14 days. On day 0, 3 and 18 blood samples were withdrawn from rats’ orbital vein for glucose measurement. All rats were sacrificed for liver, gastrocnemius muscles and pancreatic tissues collection. The liver and gastrocnemius muscle were subjected for glycogen measurement whereas pancreatic tissues were processed for histological examinations. Data was analyzed by ANOVA and followed by Duncan test. The results showed that flamboyant flowers extracts significantly (p0.05) reduced blood glucose as well as degeneration and necrosis of pancreatic β cells. Optimal dose to decrease blood glucose and pancreatic cell degeneration was 200 mg/kg BW; whereas optimum dose to decrease pancreatic cell necrosis was 400 mg/kg BW. In conclusion, flamboyant flower extract can reduce blood glucose in rats. This is the first that shows antidiabetic potential of local Flamboyant flower extracts along its toxicity effect to pancreatic tissues. These information could become a basic consideration for the use of the plant extracts as a candidate to cure patients with diabetic problems.

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