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Dr. Ir. Dyah Rini Indriyanti
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biosaintifika@mail.unnes.ac.id
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Kota semarang,
Jawa tengah
INDONESIA
Biosaintifika: Journal of Biology & Biology Education
ISSN : 2085191X     EISSN : 23387610     DOI : -
Core Subject : Agriculture,
Biosaintifika Journal of Biology & Biology Education, an electronic international journal, provides a forum for publishing the original research articles, review articles from contributors, and the novel technology news related to biological research and biological education.
Arjuna Subject : -
Articles 1,068 Documents
The Effectiveness of Local Plants from Lom and Sawang Ethnics as Antimalarial Medicine Henny Helmi; Budi Afriyansyah; Wiwied Ekasari
Biosaintifika: Journal of Biology & Biology Education Vol 8, No 2 (2016): September 2016
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v8i2.5437

Abstract

Native people or ethnic societies that live in endemic malaria islands such as in Bangka Island and Belitung Island have used many medicinal plants to cure malaria. Leaves of kesembung (Scaevola taccada (Gaertn Roxb), roots of kebentak (Wikstroemia androsaemofolia Decne), and roots of medang mencena (Dapniphyllum laurinum (Benth) are the examples. This research was aimed to investigate the present of some biochemical compound and evaluate the antimalarial activity of ethanol extract of the plants against Plasmodium falciparum 3D7 in vitro. The IC50 level was determined through visual observation under microscope over 5000 of giemsa-stained erythrocytes then analyzed by probit analysis. Results showed that kebentak root ethanol extract was effective to inhibit P. falciparum 3D7 with level 0.485 µg/mL. Furthermore, the IC50 level of kesembung leaves and medang root were 44.352 µg/mL and 1486.678 µg/mL respectively. Phytochemical test result showed that kebentak leaf ethanol crude extract contained triterpenoid, kesembung root contained phenol and tannins; moreover, medang root contained alkaloid, saponin, and triterpenoid.How to CiteHelmi, H., Afriyansyah, B. Ekasari, W. (2016). The Effectiveness of Local Plants from Lom and Sawang Ethnics as Antimalarial Medicine. Biosaintifika: Journal of Biology Biology Education, 8(2), 193-200. 
Antioxidant Activity of Jojoba (Simmondsia chinensis) Seed Residue Extract Arnold Patogi Siahaan; Eti Rohaeti; Ali Mahmoud Muddathir; Irmanida Batubara
Biosaintifika: Journal of Biology & Biology Education Vol 12, No 3 (2020): December 2020
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v12i3.23323

Abstract

Jojoba (Simmondsia chinensis (Link)) is a shrub plant that widely used as cosmetic ingredients especially jojoba oil. When making jojoba oil, the residue still reminds and become waste. This study aimed to determine the antioxidant activity of jojoba seed residue (JSR). Jojoba seed residue was collected from Sudan. The JSR extracted by maceration with n-hexane, ethyl acetate, and 70% ethanol solvent. The antioxidant activity was determined using DPPH (2,2-diphenyl-1-picrylhydrazyl) assay. The result showed that 70% ethanol extract of JSR had the highest antioxidant activity with 50% Inhibition Concentration (IC50) value of 219.42 mg/L. The 70% ethanol extract was fractionated by using silica gel column chromatography with gradient elution produced 10 fractions. All fractions showed antioxidant activity (IC50106-447 mg/L). Fraction 3 had the highest antioxidant activity with IC50 value of 106.26 mg/L. Therefore, JSR has a great potential for use as a raw material in cosmetic and pharmaceutical products industry, or as a source of bioactive compounds.
Vegetation and Community Structure of Mangrove in Bama Resort Baluran National Park Situbondo East Java Sucipto Hariyanto; Akhmad Kharish Fahmi; Thin Soedarti; Emy Endah Suwarni
Biosaintifika: Journal of Biology & Biology Education Vol 11, No 1 (2019): April 2019
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v11i1.19111

Abstract

Ecotourism development program at Bama beaches area require baseline data of mangrove structure at Bama Resort and in the past two decades has been lost about 35% area of mangrove forest in Indonesia and in the worldThe aims of this study was to find structure, composition, distribution and zonation patterns of mangroves at Bama Resort Baluran Nasional Park. Ten belt-transects were laid perpendicular to the shoreline, using standard methods. Vegetation structure was determined using data collected on plant species diversity, density, basal area, and the number of each species of mangroves. Shannon Wiener index to calculated diversity, evennes and Simpson to calculated dominance index. The results show there are 2 families and 6 mangrove species occurring in the study areas that is Rhizophoraceae (Rhizophora stylosa, Rhizophora mucronata, Rhizophora apiculata, Bruguiera gymnorrhiza, and Ceriops tagal) and Araceae (Nypa fruticans). The highest importance value was R. apiculata (229.90%) for trees, R. apiculata (148.69%) for the sapling, and R. apiculata (244.83%) for the seedling. The diversity (H) and dominance index (C) values were moderate (1.79) and 0.521. The most dominant species was R. apiculata (C=0.487). The mangrove zonation pattern from coastline to the mainland was R. stylosa, R. mucronata, and R. apiculata, in the outer zone, respectively (zone directly adjacent to the sea); B. gymnorrhiza and C. tagal in the middle zone; and N. fruticans in the zone that adjacent to the mainland. The present study will aid in the conduct and preservation planning of mangrove forest especially at Bama coast and generally in the coastal areas of Indonesia.
Histology Structure of Lymph and Uterus of White Rat (Rattus norvegicus) Given with Nanas Bongsai (Ananas comosus var. microstachys L.) Fitmawati Fitmawati; Agus Saputra; Yohanes Yohanes; Hilwan Yuda Teruna; Dimas Pramita Nugraha; Yulis Hamidi
Biosaintifika: Journal of Biology & Biology Education Vol 10, No 1 (2018): April 2018
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v10i1.13596

Abstract

Nanas Bongsai (Ananas comosus var. microstachys L.) is an amazing herb which is used in traditional medicine by local people of Muara Lembu district as a potion to reduce pain while menstruation period for a women. The present work was designed to investigate its probable side effects on the histopathologic changes in limph and uterus tissues after treated with A. comosus var. microstachys L extract in female white rats. This is an experimental research consisted of five treatments and three repetitions. Treatment composed of two controls (P0 and P+) and given Nanas Bongsai extract with three different dosages. Histology result of lymph show that all treatments given with Nanas Bongsai with three different dosages have different diameter average of white pulp and there is no significant changes with normal control. While observation towards histology structure of endometrium thickness with dosage P1, dosage P2, dosage P3 is 206.333±33.486; 215.667±33.486; 197.667±60.871 respectively. The most thin endometrium layer found in treatment with dosage P3 compared to control P0. From this study showed that there is no toxic effect on uterus and lymph function of of Nanas Bonsai (A. comosus var. microstachys L.) at different doses, so the plant is secure for consumption by the community.
Sebaran Ruang Terbuka Hijau dan Peluang Perbaikan Iklim Mikro di Semarang Barat Dewi Liesnoor Setyowati; Sri Mantini Rahayu Sedyawati
Biosaintifika: Journal of Biology & Biology Education Vol 2, No 2 (2010): September 2010
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v2i2.1152

Abstract

The purpose of the research was detecting green open space (GOS) spread and micro climate  condition  at Semarang Barat,   with  vegetation  spread  and  street  lane  area as  the  object  of  the  research. Population  characteristic  consisted  of  vegetation  area spread, mainly on the shaded  trees composition and micro climate condition by using  purposive sampling technique. The research used descriptive ecologycal approach. The result described that Semarang Barat vegetation spread is included as sparse vegetation, predominantly  by  squat  vegetation  composition with  infrequent  tree  density. Micro climate conditon is categorized as contented climate, but on edge at the daylight.  The condition  is  infl uenced  by  least  along  the  street;  consequently  the micro  climate  of temperature is high with low humidity.  Semarang Barat GOS is 17,1%, so the spacious should be 10,0% enhanced to gain 27,1% GOS to create a better climate. It is suggested to increase squat vegetation composition and quantity along Semarang Barat streets, so vegetation diversity will recovered.Keywords: green open space, micro climate, Semarang Barat
Capability of Vitamin E as a Radioprotector in Suppressing DNA Damage Determined with Comet Assay Darlina Darlina; Lusy Dahlia A.; Zubaidah Alatas; Teja Kisnanto; Mukh Syaifudin
Biosaintifika: Journal of Biology & Biology Education Vol 9, No 2 (2017): August 2017
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v9i2.8333

Abstract

Radiation has a potent to damage cells. Radiation may act directly or indirectly on deoxyribonucleic acid (DNA) that results in the degeneration of tissues and necrotic, and thereby it needs a potent radioprotector to prevent these damages. Vitamin E is natural product known as an antioxidant which has potential as radioprotector. This research aimed to determine the capability of vitamin E with emphasized on the searching for its optimal concentration as radioprotector of DNA damage. This study used blood samples of healthy person irradiated with gamma rays at a dose of 6 Gy as the lethal dose to lymphocytes. The cocentrations of vitamin E from 0 to 0.8 mM was added into blood 15 minutes before irradiation. Isolation of lymphocytes was done using gradient centrifugation method. Evaluation on the capability of this compound in suppressing DNA damage was done by using alkaline Comet assay and data analysis was done using CaspLab program. The results show that addition of vitamin E could suppres these DNA damages and 0.8 mM of vitamin could reduce DNA damage up to 94.2%. We conclude that vitamin E effectively suppresed DNA damages induced by radiation. This information may benefit to the patient from negative impacts of radiotherapy.
Potensi Ekowisata di Kawasan Mangrove, Desa Mororejo, Kabupaten Kendal Haikal Hilman Fahrian; Sapto P. Putro; Fuad Muhammad
Biosaintifika: Journal of Biology & Biology Education Vol 7, No 2 (2015): September 2015
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v7i2.3953

Abstract

Desa Mororejo memiliki kawasan mangrove yang belum dimanfaatkan secara optimal. Salah satu upaya yang dapat dilakukan untuk mengoptimalisasi adalah dengan menjadikan kawasan ekowisata. Penelitian dilaksanakan dari bulan Agustus-November 2014 yang dilakukan melalui survei yang dilanjutkan dengan kegiatan pengamatan kondisi fisik kimia dan biologi kawasan, inventarisasi keanekaragaman fauna, analisis vegetasi, serta pengambilan data persepsi masyarakat lokal. Analisis data menggunakan analisis kesesuaian wisata mangrove dan analisis SWOT (Strengths–Weakness–Opportunity–Treats). Berdasarkan hasil penelitian, kawasan mangrove Desa Mororejo didominasi oleh tiga jenis mangrove yaitu Rhizophora mucronata,  Rhizophora stylosa, dan Avicennia marina. Fauna yang ditemukan antara lain burung, ikan, dan crustacea. Indeks kesesuaian untuk kegiatan wisata mangrove termasuk kategori sesuai bersyarat (63.24%). Strategi alternatif pengelolaan ekowisata mangrove yang diprioritaskan meliputi: melibatkan masyarakat lokal dalam kegiatan ekowisata (skor 2,834); meningkatkan peran serta Dinas terkait (skor 2.517); dan adanya zonasi wilayah supaya tidak terjadi gesekan dengan berbagai pihak (skor 2.25). Mangrove areas at Mororejo village have not been optimally utilized. One effort to accelerate the optimalization of the area is by empowering its ecotourism. The study was conducted in August-November 2014 using a survey method, inventory of assorted fauna, vegetation analysis, and data collection of local community perception. Data analysis was conducted by feasibility analysis and SWOT (Strengths–Weakness–Opportunity–Treats) analysis to determine the alternatives strategy in exploring the potency of eco-tourism. Based on the result of this study, mangrove areas in Mororejo were dominated by three types of mangrove, i.e. Rhizopora mucronata, Rhizopora stylosa, and Avicennia marina. Fauna found in the areas were birds, fish, and crustacean. Feasibility index for mangrove tourism at mangrove areas at Mororejo village fell to category of conditionally feasible (63.24%). The alternative strategy in managing mangrove ecotourism at Mororejo village should involve the local community in handling ecotourism activities such as fishing activitity, birdwatching, and mangrove conservation (score 2.83); and also empower the related department (score 2.52), and zonation based areas should be implemented, therefore horizontal conflict could be avoided (score 2.25).
Histomorphometry of the Duodenum of Ducks (Anas platyrhyncos) after Administration of Nanochitosan in Feed Sunarno Sunarno; Solikhin Solikhin; Kustopo Budiraharjo
Biosaintifika: Journal of Biology & Biology Education Vol 13, No 3 (2021): December 2021
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v13i3.32120

Abstract

Poultry farming business has several problems such as lack of availability on raw materials, expensive feed prices, and egg production that is not optimal. To overcome the problem of high feed prices and duck productivity, we need to add feed additives such as nanochitosan. Poultry farming business has several problems such as lack of availability on raw materials, expensive feed prices, and egg production that is not optimal. To overcome the problem of high feed prices and duck productivity, we need to add feed additives such as nanochitosan. This study aimed to analyze the effect of nanochithosan feed on the digestive function of the intestine tenue (duodenum) as indicated by histomorphometric changes in the duodenum of Tegal ducks (Anas platyrhyncos). Duodenal histomorphometry related to digestive function was measured, including changes in lumen diameter, villi height, epithelial cell thickness, and muscular layer thickness. This study used a completely randomized design consisting 5 treatments with 5 replications. The research treatments included P0 as control (basic feed without nanochitosan particle additives) and P1, P2, P3, and P4 (basic feed with the addition of nanochitosanwith the doses of 2.5; 5; 7.5; and 10 grams/kg feed respectively). The variables observed were lumen diameter, villi height, epithelial layer thickness, and duodenal muscle layer thickness. The research data were analyzed using the analysis of variance (ANOVA) with a 95% confidence level. The results showed that the addition of nanochitosan particles in the feed had a significant effect on the lumen diameter and thickness of the duodenal muscular layer (P0.05). Tegal ducks. The conclusion of this study is that nanochitosan feed additives with a concentration of 2.5-10% can improve digestive function and improve duodenal histomorphometry in the intestine tenue of Tegal ducks as indicated by an increase in lumen diameter, villi height, and the thickness of epithelial cell thickening, and muscular layer. The novelty from this study is the production and the used of nanochitosan as feed additive to repair histomorphometry of duodenum in intestine tenue of Tegal ducks. From this research, people gets benefit by knowing the use nanochitosan as feed additive to improve digestion performance and productivity of Tegal ducks.
Microbial Succession and Chemical Characteristics in Fermentation of Ambonese Arrack (Sopi), Traditional Beverage from Maluku Ferymon Mahulette; Dea Indriani Astuti
Biosaintifika: Journal of Biology & Biology Education Vol 12, No 2 (2020): August 2020
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v12i2.23791

Abstract

Ambonese arrack is one of the traditional fermented beverage product in Maluku, Indonesia. The microbiological research of this beverage that is processed using coconut sap as raw material has never been done before. The research aimed to analyze the microbial succession and chemical characteristics during fermentation of Ambonese arrack. The sample of coconut sap was taken from traditional producer in Mahia village, Ambon. The dominant microbes in the fermentation of Ambonese arrack were Pichia polymorpha and Kloeckera javanica. The highest numbers of these two microbes was obtained after 15 hours fermentation (9.6 log Cfu/mL and 9.9 Cfu/mL, respectively). The sugar content decreased from 593.3 mg/L to 474.3 mg/L, whereas ethanol content increased from 0.0018 g/L to 0.0100 g/L. The pH value decreased from 4.70 to 3.10. The research has isolated indigenous microbes in Ambonese arrack fermentation which was considered as novelty. The bacteria that play a role in fermentation can be used as a starter in the fermentation of various beverage products, especially Ambonese arrack. The results of this research can improve the quality of this fermentation product in the future.
Barcoding of Fin Clip Samples Revealed High Exploitation of Plectropomus leopardus in Spermonde Archipelago Agus Nuryanto; Hendro Pramono; Kusbiyanto Kusbiyanto; Muhammad Iqbal Ghifari; Novi Andareswari
Biosaintifika: Journal of Biology & Biology Education Vol 10, No 3 (2018): December 2018
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v10i3.16142

Abstract

Spermonde Archipelago in South Sulawesi is among groupers sources in Indonesia for International Marine Live Trade. It was not much known which species are mostly exploited in those areas during the study because only fin clip samples could be obtained. Here we performed DNA forensic analysis to groupers fin clips collected from Spermonde Archipelago using barcode marker to know what species were exploited and which ones was the most exploited species. A total of 110 fin clip samples were obtained during the field trips. Sequencing of the cytochrome c oxidase 1 gene was successful for only 36 samples. Species determination was based on sequences similarity to conspecific sequence available in barcoding of life database (BOLD). BOLD similarity test placed the 36 fin clip samples into four different species, namely Cromileptes altivelis, Epinephelus ongus, Plectropomus leopardus, and Plectropomus maculatus. The K-2P taxonomic tree showed clear separation among species which was supported by high K2P genetic distances among species. Most of fin clips were identified as Plectropomus leopardus indicates that this species was the most exploited in Spermonde Archipelago. This proved that molecular identification not only can be used to biodiversity study in certain area but also in forensic analysis of a threatened wildlife. This study has important contribution for conservation effort of those groupers species, especially P. leopradus and provides new approach for taxonomist and conservation managers to identify samples, especially when only incomplete specimens are available.

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