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Biosaintifika: Journal of Biology & Biology Education
ISSN : 2085191X     EISSN : 23387610     DOI : -
Core Subject : Agriculture,
Biosaintifika Journal of Biology & Biology Education, an electronic international journal, provides a forum for publishing the original research articles, review articles from contributors, and the novel technology news related to biological research and biological education.
Arjuna Subject : -
Articles 1,068 Documents
Agronomic Characterization of Wheat Mutants (Triticum aestivum) of M3 Generation Planted in Sukabumi Laela Sari; Agus Purwito; Didy Sopandie; Ragapadmi Purnamaningsih; Enny Sudarmonowati
Biosaintifika: Journal of Biology & Biology Education Vol 8, No 3 (2016): December 2016
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v8i3.6612

Abstract

The purpose of this study was to identify the selection criteria to obtain a superior mutant derived from the wheat plants of such varieties as Dewata, Selayar and Alibey, adaptive in medium land. The analysis of agronomic growth characters showed a significantly effect on a growth percentage of the initial growth (8 mutants), flowering time (1 mutant), panicle stem length (15 mutants), number of panicles (7 mutants), the number of grains per panicle (8 mutants), grain weight observed (8 mutants), grain weight per genotype (6 mutants), leaf area (2 mutants) and leaf greenness (5 mutants). The effects on the characters of ripe time, harvest, panicle length and plant height were not significant. The mutants of Dewata, Selayar and Alibey could be selected based on the characters of panicle stem length, number of grains per panicle and grain weight per observation because these characters generated more mutants than the other characters. The correlation analysis between the characters of growth and yield components of wheat mutants showed that the number of grains per panicle was positively correlated with the grain weight observed, while the length of panicle stem was positively correlated with grain weight per genotype, number of panicles and leaf area. Hopefully some mutants produced could adapt to the tropical medium land, thus adding to the diversity of wheat germplasm in Indonesia, thereby reducing the import of wheat to Indonesia.How to CiteSari, L., Purwito, A., Sopandie, D., Purnamaningsih, R. Sudarmonowati, E. (2016). Agronomic Characterization of Wheat Mutants (Triticum aestivum) of M3 Generation Planted in Sukabumi. Biosaintifika: Journal of Biology Biology Education, 8(3), 353-361. 
Diversity of Pathogenic Fungi and Disease on Vegetable Crops at Polyculture Systems Eddy Tri Sucianto; Muachiroh Abbas
Biosaintifika: Journal of Biology & Biology Education Vol 13, No 2 (2021): August 2021
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v13i2.26987

Abstract

Vegetables polyculture system is potentially increasing pathogenic fungi diversity because various plant hosts are available. There is no data about patogenic fungi diversity at polyculture vegetable farming in Serang village, District of Karangreja, Purbalingga Regency. This study aimed to determine patogenic fungal diversity and disease percentage caused by the fungi at polyculture vegetable farming in Serang village, District of Karangreja, Purbalingga Regency. This research used purposive random sampling. Infected plants were collected at ten polyculture farming locations and fungal identification was performed at the laboratory. Fungi were identified morphologically based on the signs, symptoms, as well as macroscopic and microscopic characters. The fungi's pathogenity was determined by applying Koch's postulate test. The data were analyzed descriptively through literature comparison. The results showed that seven fungal species were found at polyculture farms in Serang Village. The obtained fungi were Colletotrichum sp., Fusarium sp., Alternaria sp., Septoria sp., Cercospora sp., Botryodiplodia sp., and Nigrospora sp. The lowest damage was 18.24% on tomato fruit infected by Fusarium sp. and the highest was on chili plants which was caused by Colletotrichum sp. The data is the first report for polycuture system. The obtained data has important implication for the management of vegetables farming in Serang Village.
Growth Performance, Yield Component and Genotypic Response of Mungbean (Vignaradiata l.) Against Soil Borne Diseases Emerensiana Uge; Rudi Iswanto; Ratri Tri Hapsari; Trustinah Trustinah
Biosaintifika: Journal of Biology & Biology Education Vol 12, No 1 (2020): April 2020
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v12i1.23827

Abstract

Soil-borne disease caused by fungus infection is one of the disease that causes the death and crop failure of mungbean. The aim of this study was to identify the performance, yield component and respons of plant to the disease. Performance of 25 genotypes were observed in installations research and assessment of agriculture technology (IPPTP), Jambegede of Indonesian Legumes and Tuber Crops Research Institute (ILETRI)at dry season 2018 using RCBD with three replications. The result showed that each variety has different characteristic. The plant with the highest number of plant height was genotype 15 and 18, while the total branches of 25 genotypes were generally similar (4).The highest number of grown and harvested plant was genotype 15. The genotypes with shorter flowering, maturity and harvesting age were 23, 20, 18, 15 and 10. The highest average number of filled pods was genotype 11 and empty pods was genotype 13. There were 8,10 and 2 genotypes with seeds weight of 7, 6 and 6 respectively. The highest yields were obtained from genotype 19 and 3 by total of yield of 2.0 t/ha and 1,9 ton/ha respectively. Resistance test against soil-borne diseases resulted 5 genotypes that were moderate resistant observed at 2 DAP. This research contributed to gave information about new genotypes of mungbean that potentially produce new superior varieties.
Relationship of Bird Diversity and Plant Composition Inside The Area Campus Green Space of Universitas Padjadjaran Jatinangor, Sumedang West Java Deden Nurjaman; Teguh Husodo; Erri Noviar Megantara; Herri Y. Hadikusumah; Indri Wulandari
Biosaintifika: Journal of Biology & Biology Education Vol 10, No 3 (2018): December 2018
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v10i3.13543

Abstract

Padjadjaran University (UNPAD) Jatinangor is currently conducting green Campus program. To support the program, a study of biota living in it, as one of the benchmarks of good or bad environmental conditions, is needed. The green space of Jatinangor Campus is divided into two clusters namely Cluster I green space (Campus Forest) and green space Cluster II (Campus Non Forest). The objective of the research was to know the relationship between diversity of birds with diversity of plants in the green space of Cluster I (Campus Forest) and Cluster II (Campus Non Forest) UNPAD Campus Jatinangor as one of the parameters of successful development of green Campus. This research is descriptive-explorative with census method on bird species and plant composition from green spaces of Cluster I (Campus Forest) and Cluste II (Campus Non Forest) Campus UNPAD Jatinangor. From the observations in Cluster I, we identified 46 species of birds and 77 species of plants, whereas in Cluster II, we identified 32 species of birds and 74 types of plants. The number of bird species is directly proportional to the number of plant species from Cluster I and Cluster II green spaces. From this study it was concluded that the diversity of tree species supports the diversity of bird species.
Peranan Faktor Imun dan Profil Protein dalam Penelitian dan Pengembangan Vaksin Malaria Iradiasi Mukh Syaifudin
Biosaintifika: Journal of Biology & Biology Education Vol 6, No 1 (2014): March 2014
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v6i1.2929

Abstract

Pengembangan vaksin terhadap malaria yang merupakan penyakit yang mematikan tetap menjadi satu prioritas kesehatan masyarakat global, termasuk pemanfaatan parasit iradiasi sebagai bahan vaksin. Imunisasi dengan sporozoit iradiasi mampu memberikan imunitas protektif pada hewan coba dan sukarelawan. Mekanisme sistem kekebalan tubuh ini banyak dipelajari karena merupakan faktor penting dalam pengembangan vaksin, demikian halnya profil dan/atau ekspresi protein pasca iradiasi yang terkait erat dengan keamanan dan aspek lain dari bahan vaksin. Meskipun telah melalui penelitian yang ekstensif, vaksin yang aman dan protektif belum dapat diperoleh karena masih diperlukan pengetahuan yang lebih mendalam mengenai mekanisme imunitas dan protein dalam litbang malaria. Hasil penelitian menunjukkan bahwa sel limfosit T berperan penting dalam pengaturan respon imun dan pembentukan memori imunologik yang mengontrol dan mengeliminasi infeksi. Sitokin proinflamasi seperti interleukin-12 (IL-12), interferon-gamma (IFN-γ), dan tumor necrosis factor alpha (TNF-α) juga merupakan mediator esensial dari imunitas protektif pada malaria eritrositik. Berbagai pendekatan lain terkait respon imun seperti genetika molekuler saat ini sedang dilakukan. Studi juga menunjukkan bahwa profil protein bergantung pada beberapa faktor yang akan dibahas lebih lanjut dalam makalah. The development of vaccine against malaria as the deadly disease remains the global public health priority; and it includes the use of irradiated parasites as vaccine materials. Immunization with irradiated sporozoites could provide protective immunity in animals and volunteers. The mechanism of this body immunity system has been studied widely due to its important role in the development of vaccines and profiles and/or protein expression post-irradiation which are closely related to safety and other aspects of vaccine materials. Even though extensive research has been done, a safe and protective vaccine remains elusive because more deeply knowledge on immunity mechanism and protein in malaria research is still needed. Results showed that T-cell lymphocytes have an important role in the regulation of immune response and in the formation of immunological memory which controls and eliminates the infection. Pro-inflammatory cytokines such as interleukin-12 (IL-12), interferon-gamma (IFN-γ), and alpha tumor necrosis factor (TNF-α) are also essential mediators of protective immunity in erythrocytic malaria. Various other approaches related to immune response such as molecular genetics has been carried out. The study also showed that protein profile is depended on some factors that will be discussed further in the paper.
Ex situ Conservation Effort through the Inventory of Plant Diversity in Mount Seblat, Bengkulu Imawan Wahyu Hidayat; Ikhsan Noviady; Yati Nurlaeni
Biosaintifika: Journal of Biology & Biology Education Vol 9, No 3 (2017): December 2017
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v9i3.9668

Abstract

Mount Seblat, as part a of Kerinci Seblat National Park (KSNP), is a pristine and natural mountain, particularly from disturbances and destructions by human activities. Nevertheless, the richness of biological resources especially plant diversity.in this area has not been more explored. The purpose of this study was to conduct an inventory of plant diversity and to determine the plant species composition. The inventory activities were conducted by plants collection along the ascent route. The results were then be maintained through ex situ conservation method in Cibodas Botanical Garden (CBG). The study was conducted by exploratory method, from Seblat Ulu Village (641 m asl) up to altitude of 1,037 m asl. There were 18 points of plant sample observation with an area of 5 x 5 square meters per point. Plant collection obtained 380 specimens. Five groups of most collected plants were Lauraceae (18 species), Rubiaceae (8 species), Anacardiaceae (6 species), Annonaceae (5 species), and Fagaceae (4 species). In order to enrich the plants collection as well as conduct the ex situ conservation effort, plants from Orchidaceae were also collected which resulted in 33 species. These results were an important initial inventory of plant diversity of Mount Seblat, considering that there was no record as well as very limited current information. When the environment disturbance tends to increase, this information may act as a reference and an initial database to develop plants conservation effort and strategy in the future.
Phylogenetic Relationships of Local Durian Species based on Morphological Characteristics and PCR-RFLP Analysis of the Ribosomal Internal Transcribed Spacer (ITS) DNA Fidia Fibriana; Lutfia Nur Hadiyanti
Biosaintifika: Journal of Biology & Biology Education Vol 8, No 3 (2016): December 2016
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v8i3.6748

Abstract

In this study, twenty local durian accessions obtained from Central Java in situ collection were characterized using the morphological characteristics and the restriction patterns which generated from the region spanning the internal transcribed spacers ITS LEU and ITS 4. Morphological characteristics of durian leaf, stem, tree, and fruit showed variations for the different accessions, whereas polymerase chain reaction (PCR) products of ribosomal DNA region showed a low length of variation. The size of the PCR products and the restriction analyses with the restriction endonucleases Bsp1431yielded a restriction pattern for each accessions. The results of this study can be utilized by local durian farmers as a preliminary reference for durian propagation. The data obtained need to be supported by further research using the other molecular markers to obtain more accurate data. The clear identity of durian species can help the management of propagation systems by farmers to get superior local durian.How to CiteFibriana, F., Hadiyanti, L. N. (2016). Phylogenetic Relationships of Local Durian Species based on Morphological Characteristics and PCR-RFLP Analysis of the Ribosomal Internal Transcribed Spacer (ITS) DNA. Biosaintifika: Journal of Biology Biology Education, 8(3), 362-370. 
Ethnobotanical Study of Early Childhood Medicinal Plants Used by the Local People in South Bangka Regency, Indonesia Henri Henri; Vitryany Nababan; Luchman Hakim
Biosaintifika: Journal of Biology & Biology Education Vol 12, No 3 (2020): December 2020
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v12i3.22221

Abstract

South Bangka Regency has a long history of using medicinal plants as part of its medical system. However, the potential associated with this aspect of traditional medicine remains understudied and poorly explored. This study aimed to describe the types of medicinal plants and analyze the use of medicinal plants to treat early childhood disease by the local community of South Bangka Regency. The research method used was open interview with local people using the questionnaires. The data was analyzed using Use Value (UV), Fidelity Level (FL), and Relative Frequency of Citation (RFC). The analysis identified 55 plant species from 35 families. The most common families were Euphorbiaceae (8.92%), Fabaceae (7.14%), Poaceae (7.14%), Zingiberaceae (5.36%), and Lamiaceae (5.36%). The most widely used plant was shallot plants (Allium cepa L.) with values of UV, FL, and RFC were 0.67, 66.67, and 0.015, respectively. This study revealed the richness of ethnomedicinal knowledge in the South Bangka Regency. Finally, it is expected that this ethnobotany study can provide a database for further scientific research. The community’s knowledge as a legacy will not be repeated if it is not inherited. Besides, this biodiversity is very important as a socio-economic and ecological asset in South Bangka Regency which must be protected by all means from over exploitation.
Antioxidant and Antiglycation Activity of Rhizosphere and Endophytic Actinobacteria of Xylocarpus granatum Alifian Ariansyah; Irmanida Batubara; Yulin Lestari; Saat Egra
Biosaintifika: Journal of Biology & Biology Education Vol 11, No 2 (2019): August 2019
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v11i2.20018

Abstract

Utilization of endophytic bacteria such as actinobacteria is one of the alternatives to obtain the bioactive compounds similar to the host plant. This study aimed to identify compounds produced by rhizosphere and endophytic actinobacteria isolated from Xylocarpus granatum for their antioxidant and antiglycation activity. Actinobacterial culture supernatant was extracted using ethyl acetate with a ratio of 1:1 (v/v). Antioxidant activity was performed using 2,2-diphenyl-1-picrylhydrazyl (DPPH). Whole TLC-bioautography and phytochemical tests were used to predict the group of active compounds. Antiglycation activity was identified based on the ability to inhibit the formation of advanced glycation end products (AGEs). Fifteen actinobacterial isolates had been isolated from rhizosphere (ten isolates), fruit (two isolates), and leaf (two isolates) of X. granatum. All isolates had been tested for their antioxidant and antiglycation activity. Isolate XR2 had the highest antioxidant activity with IC50 value of 1719.26 mg/L. Meanwhile, the highest antiglycation activity was obtained from isolate XR8 with IC50 value of 327.62 mg/L. This study informs that actinobacteria also live in the fruit, leaves and rhizosphere of X. granatum. The existence of rhizosphere and endophytic actinobacteria from X. granatum and their antioxidant and antiglycation activities contributes to the understanding of their diversity and potency as an antioxidant and antiglycation agent.
Isolation of Thermophilic Bacteria from Bora Hot Springs in Central Sulawesi Slamet Ifandi; Muh Alwi
Biosaintifika: Journal of Biology & Biology Education Vol 10, No 2 (2018): August 2018
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v10i2.14905

Abstract

Thermophilic bacteria can survive at high temperature, in which hot spring is one of its habitats. Indonesia has many hot springs with potential as a habitat for thermophilic bacteria. The purpose of this study was to obtain isolates thermophilic bacteria from Bora hotspring located in Central Sulawesi. This study applied a descriptive-observational study design, characteristics of bacterial properties identified using conventional methods according to the Bergey’s Manual of Determinative Bacteriology. The study was conducted in 3 stages. The first stage was bacteria cultivation on the appropriate media, followed by stage of isolated and the last stage by identified characteristics of thermophilic bacteria which included microscopic and macroscopic morphology, Physiological and biochemistry test. The results of the isolates indicted 4 representative isolated of thermophilic bacteria from Bora Hot Spring namely TM022, TM023, TM024, TM026. The bacteria isolates obtained were bacillus, coccus and Gram negative and positive, while the physiological test of all isolates were able to grow and showed changes in the medium. This study is useful in providing characteristic of indigenous thermophilic bacteria isolates that produces thermostable enzymes.

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