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Bioma : Berkala Ilmiah Biologi
Published by Universitas Diponegoro
ISSN : -     EISSN : 25982370     DOI : -
Core Subject : Education, Agriculture,
Agriculture, Biological Sciences & Forestry Education
BIOMA (ISSN 1410-8801) adalah Majalah Ilmiah Biologi yang bertujuan mewadahi semua informasi hasil penelitian, telaah pustaka, makalah teknis, dan kajian buku, dari berbagai cabang ilmu Biologi. Diharapkan penerbitan ini dapat berkontribusi dalam penyampaian informasi ilmiah yang merupakan mata rantai proses kegiatan ilmiah. Terbit sejak tahun 1999.
Arjuna Subject : -
Articles 318 Documents
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Pengaruh Pemberian Hidrogen Peroksida (H2O2) Dalam Pengendalian Ektoparasit, Dan Kelangsungan Hidup Benih Ikan Nila Salin (Oreochromis niloticus) Di Balai Besar Perikanan Budidaya Air Payau Jepara Arini, Priesty Dyah; Muhammad, Fuad; Baskoro, Karyadi; Fahris, Noor
Bioma : Berkala Ilmiah Biologi Vol. 20, No. 1, Tahun 2018
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (202.015 KB) | DOI: 10.14710/bioma.20.1.59-65

Abstract

Nile tilapia fish (saline) is tolerable to high salinity and has a better resilience in environmental change, make it perfect for fish culture in the ponds. Problems which usually occur in fish culture is disease which is triggered by the decrease of water quality so that the parasytes begin to emerge. The ectoparasyte which usually attacks the fish is Trichodina sp. By adding hydrogen peroxide into the water medium, this type of ectoparasyte is expected to be controlled. This research is conducted to examine the influence of the hydrogen peroxide concentration difference to the increase of water medium quality, ectoparasyte controlling, and the survival rate of nile tilapia fish. The used method is by adding hydrogen peroxide into the water medium in various concentration, 0 ppm as control, 5 ppm, 10 ppm, and 15 ppm given in three times until day 10. The result of this research shown that different hydrogen peroxide concentration may influence the quality of the water medium, controling ectoparasyte,  increase nile tilapia survival rate. The medium with hydrogen peroxide concentration (15 ppm) shows the best result. Keywords: nile tilapia fish, Trichodina sp, hydrogen peroxide, water medium quality, ectoparasyte control, survival rate.
Potensi Chlorella vulgaris Beijerinck Dalam Remediasi Logam Berat Cd Dan Pb Skala Laboratorium. Purnamawat, Florensia Setyaningsih; Soeprobowati, Tri Retnaningsih; Izzati, Munifatul
Bioma : Berkala Ilmiah Biologi Vol. 16, No.2, Tahun 2014
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (190.676 KB) | DOI: 10.14710/bioma.16.2.102-113

Abstract

Salah satu dampak negatif modernisasi dan industrialisasi adalah pencemaran lingkungan. Perairan merupakan salah satu lingkungan yang paling terbebani bahan pencemar karena banyaknya limbah rumah tangga maupun industri yang akhirnya masuk ke lingkungan perairan. Salah satu bahan pencemar perairan yang paling membahayakan adalah logam berat karena bersifat non-biodegradable. Oleh karena itu perlu upaya penanganan maupun pencegahan terhadap bahan pencemar tersebut. Pengolahan limbah secara fisiko-kimiawi dinilai mahal, menurunkan biodiversitas, banyak lumpur yang dihasilkan, dan kurang efektif pada konsentrasi logam di bawah 50 mg/l. Bioremediasi merupakan salah satu metode perbaikan lingkungan yang lebih ramah lingkungan karena menggunakan agen hayati seperti bakteri, jamur, protista, dan tanaman. Chlorella vulgaris Beijerinck merupakan mikroalga bersel satu yang banyak tumbuh di perairan tawar dan laut, telah dimanfaatkan masyarakat sebagai bahan pakan, suplemen, biofuel dan bioremediasi. Penelitian ini bertujuan untuk menguji potensi C. vulgaris sebagai agen bioremediasi terhadap cemaran logam berat Cd dan Pb skala laboratorium. Penelitian menggunakan Rancangan Acak Lengkap (RAL). C. vulgaris ditumbuhkan dalam media kultur yang telah diberi pupuk Walne selama 76 hari. Media kultur ditambah ion logam Cd dan Pb dengan 3 konsentrasi yang berbeda yaitu  1 ppm, 3 ppm, dan 5 ppm, masing-masing 3 kali ulangan. Medium kultur tanpa penambahan logam dianggap sebagai kontrol. Kandungan logam berat dalam medium dan dalam sel C. vulgaris diukur dengan AAS. Hal yang diamati dalam penelitian ini adalah pola pertumbuhan populasi C. vulgaris, persentase penurunan logam Cd dan Pb oleh C. vulgaris, besarnya akumulasi logam dalam C. vulgaris, serta nilai Bioconcentration Factor (BCF). Berdasarkan penelitian tersebut terbukti bahwa C. vulgaris terbukti mampu menurunkan konsentrasi ion Cd dan Pb dalam perairan. Prosentase penurunan konsentrasi ion Pb dalam media kontrol, 1 ppm, 3 ppm, dan 5 ppm berturut-turut  70%, 80%, 62%, dan 52% sedangkan dalam media Cd pada konsentrasi serupa berturut-turut 67%, 79%, 56%, dan 51%. C.  vulgaris mampu mengakumulasi  Cd lebih besar daripada Pb. Berdasarkan nilai BCF  terhadap  Cd maupun Pb, C. vulgaris tergolong akumulator logam.   Kata kunci : logam berat, bioremediasi, Chlorella vulgaris,  bioakumulasi.
Pengaruh Penambahan Limbah Kertas 80% dan Kayu 20% Sebagai Alternatif Media Tanam Jamur Tiram Putih (Pleurotus ostreatus) Isnawati, I; Mahmudi, Irkham; Khayati, Dian N; Utami, Tri W; Purwanti, Kiki E; Ulfa, Maria
Bioma : Berkala Ilmiah Biologi Vol. 21, No 2, Tahun 2019
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (423.687 KB) | DOI: 10.14710/bioma.21.2.139-145

Abstract

Paper waste is an  organic residual that has not been widely utilized and its existence around us. The high cellulose content in paper waste allows it to be used as a media for cultivating white oyster mushrooms. This aims to compare the speed of mycelium propagation from different concentration of paper in each growing medium. The study used a Completely Randomized Design (CRD) with six treatments and five repeattation. The treatment used is the difference in the composition of 0% paper waste media (control); 20% of paper waste; 40% of paper waste; 60% of paper waste; 80% of paper waste; and 100% of waste paper. The data were obtained from six time  observations in the form of mycelium propagation (cm)and tested with anava. The results were not significant at 1,65 using correcttion factor 2,53. Mycelium propagation speed was influenced by environmental factors such as temperature, light intensity, humidity, and air circulation.
Perbedaan Somatometri Itik Tegal, Itik Magelang Dan Itik Pengging Wulandari, Desi; Sunarno, Sunarno; Saraswati, Tyas Rini
Bioma : Berkala Ilmiah Biologi Vol. 17, No.2, Tahun 2015
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (131.158 KB) | DOI: 10.14710/bioma.17.2.94-101

Abstract

Local ducks in Indonesia there are several kinds, for example Tegal ducks, Magelang ducks and Pengging duck each having characteristics different somatometri. Research on somatometri ducks that includes body weight, leg length, beak length, wing length, body length, and long-neck ducks important to know somatometri of each duck. This study aimed to analyze the differences somatometri at Tegal ducks, Magelang ducks and Pengging duck, which includes weight, leg length, beak length, wing length, body length, and a long neck. Ducks were used in this study maintained in the Central Breeding and Raising Ducks Work Unit Non Ruminant Livestock Banyubiru, Ambarawa. The study was conducted by measuring somatometri of each six monthold ducks. Duck samples taken from three different types of ducks that Tegal duck, Pengging duck and Magelang duck, of each type of sample taken many ducks as 6 tails. Data were analyzed using analysis of variance (ANOVA) with the differentiating factor is the type of duck. If the data were analyzed contained a real difference, then do a further test using the test Least Significant Differences (LSD) at 95% confidence level. The results showed that there was no significant difference in all parameters of the study. Conclusion of the study three types of ducks will have somatometri not unlike that includes, weight, leg length, beak length, wing length, body length and the length of the neck. It was because give feed and feed same time. Keywords: Local Ducks in Central Java, Somatometri, Tegal ducks, Magelang ducks, Pengging duck
Studi Awal Upaya Eksplorasi Agensia Imunokontrasepsi Untuk Regulasi Fertilitas Hewan Liar : Pofil Protein Selama Proses Implantasi Embrio Mencit (Mus musculus L.) BALB/c. Sitasiwi, Agung Janika; Djaelani, Muhammad Anwar
Bioma : Berkala Ilmiah Biologi Vol. 13, No. 1, Tahun 2011
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (95.169 KB) | DOI: 10.14710/bioma.13.1.39-45

Abstract

Study on exploration of immunocontraception agents for wildlife fertility regulation has been conducted.The aim of this study was carried to find out the protein which functionable in mice embryos implantation. Theoutcome of this study should be applied to control the fertility of wildlife animals. This research conducted duringten months in BSF Laboratory of Biology Department FMIPA UNDIP and 3rd Unit of UGM LPPT. Adult femaleand male mice with 28 – 30 grams in weight were used as laboratory animals. Mice were divide into two groups, onegroup as positive control group without mated, the other were mated group. Mice handled and breeded in laboratorycondition. The precise day of pregnancy were determined with vaginal plug existences in female mice. Protein wereisolated from uterus on day 1st to 5th of pregnancy. Protein isolation and separation with electrophoresis were basedon Bio-rad manual. This study show that there are no differences in protein band between positive control group andpregnant group. These study suggested that protein determining embryo implantation process is regulatory proteinwhich it has expressed in short time and fast withdrawl.
Pertumbuhan Kelinci (Lepus spp.) Setelah Pemasangan Implan Stainless Steel AISI 316L Wicaksono, Wisnu Adi; Tana, Silvana; Mardiati, Siti Muflichatun
Bioma : Berkala Ilmiah Biologi Vol. 18, No.2, Tahun 2016
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (116.147 KB) | DOI: 10.14710/bioma.18.2.137-143

Abstract

A lot of accidents can cause fractures/cracks, especially in the thigh bone. Bone fractures/cracks can be overcome through implants installation (implantation) particularly on the part of the femur. A test take the implant integration process about 4-6 week. Research on animals is performed the rabbit (Lepus spp.) implantation of stainless steel AISI 316L. The implantationof stainless steel AISI 316L implant was on the right femur between thigh bone and sceletal muscle, that was observed for 1,5 months. Parameters observed in this research were body weight gain, feed consumption, conversion of feed, and drinking consumption. Test used is t two test samples independent two sides with take samples 5 control and 5 treatment stainless steel AISI 316L. The results show the stainless steel AISI 316L implantsignificant differences body weight gain, feed consumption, and drinking consumption,but no significant different feed conversion. The conclusion is an implant stainless steel AISI 316L influence body weight gain rabbits after implantation for 1.5 months. Keywords: Rabbit, stainless steel AISI 316L, growth, body weight
Pola Aktivitas HarianPasangan Burung Serak Jawa (Tyto alba) di Sarang Kampus Psikologi Universitas Diponegoro Tembalang Semarang Hadi, Mochamad -
Bioma : Berkala Ilmiah Biologi Vol. 10, No. 1, Tahun 2008
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (627.651 KB) | DOI: 10.14710/bioma.10.1.23-29

Abstract

Burung serak jawa (Tyto alba) merupakan hewan nocturnal. Burung T. alba biasanya bersarang padabangunan tua dan gedung yang tinggi dengan menempati sarang yang sudah ada atau mengambil alih sarang yangditinggalkan burung lain. Kemampuan bersarang pada gedung dan bangunan ini menyebabkan T. alba mampumenempati gedung di kampus Psikologi Universitas Diponegoro Tembalang Semarang. Sifat T. alba untukmenempati gedung kampus Psikologi Universitas Diponegoro dapat mempermudah perkembengbiakan, namunpotensi untuk perjumpaan dengan manusia juga sangat besar. Penelitian ini bertujuan untuk mengetahui poladistribusi waktu yang digunakan oleh Tyto alba untuk aktivitas hariannya. Teknik pengamatan scaning samplingdigunakan sebagai dasar penentuan alokasi waktu untuk aktivitas burung hantu T. alba seperti, terbang, bertengger,berburu, istirahat, bercumbu dan bertarung. Pengamatan dilaksanakan selama 14 X 24 jam, dengan 12 jam hariterang dan 12 jam hari gelap. Penentuan pola aktivitas harian dengan menghitung rata – rata total aktivitas T. albaselama 24 jam. Aktivitas harian yang paling banyak dilakukan oleh pasangan T. alba adalah aktivitas istirahat (14jam 44 menit) dengan puncak waktu istirahat pada siang hari. Waktu yang dihabiskan untuk istirahat T. alba betinalebih tinggi (15 jam 53 menit). Alokasi waktu yang digunakan untuk bertengger T. alba betina (2 jam 56 menit)sebagai bentuk proteksi terhadap sarang. T. alba jantan bertanggung jawab memberikan kebutuhan nutrisi pada T.alba betina waktu yang digunakan untuk berburu (7 jam 40 menit). Pasangan T. alba melakukan aktivitas bertarungsecara bersamaan (5 menit) dan waktu untuk bercumbu (4 menit).
Isolasi dan Identifikasi Bakteri Pelarut Fosfat dari Tanah Gambut Larasati, Ella Dewani; Rukmi, MG Isworo; Kusdiyantini, Endang; Ginting, R Cinta Badia
Bioma : Berkala Ilmiah Biologi Vol. 20, No. 1, Tahun 2018
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (383.652 KB) | DOI: 10.14710/bioma.20.1.1-8

Abstract

Phosphate (P) are macronutrients that necessary for growth and development of plants, But the availability of phosphate dissolved in the ground are very limited because trend is bound to minerals ground form phosphate complexs. One of the alternative to improve the efficiency of phosphate is by utilize phosphate solubilizing bacteria. Phosphate solubilizing bacteria is bacteria capable of dissolving phosphate that is not available become available, so that it can be absorbed by plants. Peat soils can be used as a source of phosphate solubilizing bacteria because , peat soils formed from litter and organic matter so, many microorganisms which live in peat soils one of them is a phosphate solubilizing bacteria. The aim of this research is to isolat, identify bacteria to species level using Biolog system GenIII Mircoplate, and test the ability of phosphate dissolution. The isolation is done with the methods spread plate in a media pikovkaya solid and testing the ability to isolat qualitatively glimpsed clear zone around colonies, measuring phosphate solubilizing index. testing the ability isolats quantitatively by measuring solubility phosphate using spektrofotometer, and identification isolats using Biolog System. Eighteen isolates phosphate solubilizing bacteria successfully obtained from of peat soils, two isolates of them potential in solubilizing phosphate, According to Biolog system the second isolates identified as Pseudomonas tolaasii (isolate PG2T.5) and a Bacillus pumilus (isolate PG3TT.2), each is about phosphate dissolved successive of 24,81 mg/L and 22,62 mg/L Key words: Peat Soil, Isolation, Phosphate Solubilizing  Bacteria, Biolog System Identification
Eksplorasi Jamur Alkalotoleran dari Desa Sukolilo Barat, Kecamatan Labang, Kabupaten Bangkalan, Madura, Jawa Timur Lusiana, Helga; Rukmi, MG Isworo; Raharjo, Budi
Bioma : Berkala Ilmiah Biologi Vol. 16, No.1, Tahun 2014
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (3031.181 KB) | DOI: 10.14710/bioma.16.1.10-17

Abstract

Mold exploration from limestone hills West Sukolilo  village, District Labang, Bangkalan, Madura, East Java,  conducted to found the  alkalotolerant mold isolates and investigate their  amylolytic, proteolytic, and cellulolytic activity. Mold isolation were done using spread plate and dilution method  on  PDA and CMA at pH 8.0, 9.0, and 10.0. Twenty-seven mold isolates have been found consist of  four genus  i.e. Aspergillus, Fusarium, Curvularia, and Trichoderma. The result of amylolytic test  showed Trichoderma longibrachiatum (IMD-26) have the  highest activity at pH 8, while  Fusarium sp 2 (IMD-24) showed the highest activity at pH 9. Aspergillus flavus (IMD-7) showed highest proteolytic activity at pH 8, while Hyphomycete (IMD-27) showed the highest activity at pH 9. The highest cellulolytic activity at pH 8 showed by Trichoderma harzianum (IMD-25) and pH 9 by Fusarium sporotrichoides (IMD-20). Keyword : alkalotoleran mold, amylolytic, proteolytic, cellulolytic.
Phytochemicals Screening and Effectiveness of Free Radical Inhibitors of Garlic (Allium sativum L.) Ethanol Extract from Timor Island Priska, Melania; Peni, Natalia; Carvallo, Ludovicus
Bioma : Berkala Ilmiah Biologi Vol. 21, No 1, Tahun 2019
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (60.947 KB) | DOI: 10.14710/bioma.21.1.72-77

Abstract

The purpose of the study was to determine the content of secondary metabolites and the effectiveness of garlic (Allium sativum L.) ethanol extract from Timor island which has the potential to inhibit free radicals. The method used for testing secondary metabolites is phytochemical screening using color reagents. The effectiveness of free radical inhibitors from the ethanol extract of garlic (Allium sativum L.) from Timor island was carried out in two steps are: 1.) Determination of DPPH wavelength (λ) maximum and 2.) Measurement of antioxidant activity using the DPPH method. The results showed the ethanol extract of garlic (Allium sativum L.) from Timor Island contained secondary metabolites of flavonoids, phenols, and terpenoids. The ethanol extract of garlic (Allium sativum L.) from Timor Island also has strongest effectiveness in inhibiting free radicals, with the acquisition of IC50 values <50 ppm which is equal to 9,729 ppm.

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