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AL-HAYAT: Journal of Biology and Applied Biology
Published by Universitas Islam Negeri Walisongo Semarang
ISSN : 26543702     EISSN : 26220725     DOI : -
Core Subject : Science,
Biochemistry, Genetics & Molecular Biology
AL-HAYAT: Journal of Biology and Applied Biology an international journal focuses on biological science, biotechnology, bioremidiation, environmental biology, and biological education. It is intended to communicate original research and article review in current issues on the subject. The subject covers field studies and literary with various perspectives, both interdiscipline and multidiscipline, i.e. ecology, technology, education and much more. This journal is published by Fakultas Sains dan Teknologi, Universitas Islam Negeri (UIN) Walisongo Semarang Indonesia, and managed to be issued twice in every volume. We warmly welcome contributions from scholars and researchers of many disciplines.
Arjuna Subject : -
Articles 8 Documents
 1 
Search results for , issue "Vol 7, No 1 (2024)" : 8 Documents clear
Molecular characterization of heavy metal tolerant bacteria from an agricultural soil Ilusanya, Olusolape Afolake Favour; Olajumoke, Oluwasegun Irouoghene; Fogbonja, Oyindolapo Christianah; Egberongbe, Haneefat Olabimpe; Oyeyipo, Funmilayo Mujidat; Yakubu, Reuben
Al-Hayat: Journal of Biology and Applied Biology Vol 7, No 1 (2024)
Publisher : Fakultas Sains dan Teknologi, UIN Walisongo Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21580/ah.v7i1.20474

Abstract

The aim of this study was to isolate and characterize heavy metal tolerant bacteria from an agricultural soil in Ago Iwoye, Ogun Stae, Nigeria. Bacteria were isolated from soil samples collected and screened for tolerance to different concentrations of lead, copper and cadmium. Heavy metal tolerant bacteria were characterized using phenotypic and molecular techniques. All the isolates were able to grow and tolerate different concentration of the heavy metals while four isolates identified as Bacillus cereus, Pseudomonas aeruginosa, Altantibacter hermannii and Enterobacter quasihormaechei exhibited high degree of tolerance to Pb, Cu and Cd with minimum inhibitory concentrations of 1500mg/l of lead,1000mg/l of Cu and 700mg/l of Cd. These results highlighted the high potential of these bacteria as bioremediation tools of heavy metal contaminated soil.Keywords: Heavy metal, tolerance, bacteria, agricultural soil
Arthropod diversity in three different habitats around Sumbawa University Of Technology, Sumbawa, NTB Fikri, Galih El; Arrufitasari, Putri Nur
Al-Hayat: Journal of Biology and Applied Biology Vol 7, No 1 (2024)
Publisher : Fakultas Sains dan Teknologi, UIN Walisongo Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21580/ah.v7i1.18182

Abstract

Arthropods have an important role in a habitat and food chain cycle. Identification of arthropod species abundance and diversity is done as a biological indicator of the environment. The aim of the research is determine the diversity and community structure of soil arthropods in various habitats and provide information on environmental quality caused by human activities around the campus of Sumbawa University of Technology. This research was conducted in three locations in the area around the Sumbawa University of Technology, Batu alang village, Moyo Hulu sub-district, Sumbawa district. The three locations include hillsides, campus areas and corn fields. Sampling of soil arthropods using the pitfall trap technique. Data on abundance, species richness, diversity, Calculation of the Index of Importance (INP) and diversity value (H') was analized by Excel. The results of Arthropod identification found a total of 2083 individuals consisting of 20 families and 8 Arthropod orders. The three sites showed that the abundance of arthropods in campus area was the highest at (212.75 ± 9.54) Arthropod species richness in corn field was the highest at 2.67 ± 0.4 Arthropod diversity (H’) in hilside was the highest at (3.23 ± 0.16). Campus area and corn field which have higher similarity.
[Retracted] GC-MS Study of Bioactive Compound of Peperomia pellucida and Its Antibacterial Activity against Streptococcus mutans Kuswati, Kuswati; Rosalinda, Julitha Dwi; Waluyo, Joko
Al-Hayat: Journal of Biology and Applied Biology Vol 7, No 1 (2024)
Publisher : Fakultas Sains dan Teknologi, UIN Walisongo Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21580/ah.v7i1.18877

Abstract

Streptococcus mutans is the prior bacteria causing dental caries. Suruhan (Peperomia pellucida) may prevent dental caries due to its antibacterial bioactive content. This study aims to identify the bioactive profile of Suruhan ethanol extract and its antibacterial activity against Streptococcus mutans. Bioactive compounds were analyzed using Gas Chromatography-Mass Spectrometry (GC-MS). Antibacterial activity was tested through an inhibition test using the well-diffusion method, the Minimum Inhibitory Concentration (MIC) test, and the Minimum Bactericidal Concentration (MBC) test. The most detected compounds in Suruhan ethanol extract were n-eicosane, n-hexadecane, and glycerol. There are also antibacterial bioactives such as phenols, flavonoids, alkaloids, and terpenoids. Inhibition test of 500 mg/mL extract showed a clear zone of 8.25 mm diameter. The MIC and MBC values were 50 mg/mL and 100 mg/mL, respectively. Based on the results, Suruhan ethanol extract shows potential as an antibacterial, although its inhibitory effectiveness could be better. 
A Review of Termite for Sustainable Green Building Kusumawardhani, Dina Tiara; Almulqu, Aah Ahmad
Al-Hayat: Journal of Biology and Applied Biology Vol 7, No 1 (2024)
Publisher : Fakultas Sains dan Teknologi, UIN Walisongo Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21580/ah.v7i1.20704

Abstract

Although 3106 termite species have been documented worldwide, little information is available on those in Indonesia. In this review, the Google Scholar search platform was used to obtain information on termite species. From the 34 reviewed sources, they were identified in 4 provinces across Indonesia. Among these sites, we found 106 termite species from the islands of Sumatera, Kalimantan, Sulawesi, and Java. The most predominant species in all regions were Macrotermes sp, Microtermes sp, and Coptotermes sp.
Variations in Food Choices of Long-Tailed Monkeys (Macaca fascicularis) in the Pulaki Temple Area, Gerokgak District, Buleleng Regency Syah, Moh Jafron; Yuliastuti, Yuliastuti; Safitri, Mely
Al-Hayat: Journal of Biology and Applied Biology Vol 7, No 1 (2024)
Publisher : Fakultas Sains dan Teknologi, UIN Walisongo Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21580/ah.v7i1.20674

Abstract

AbstractMacaca fascicularis, included in the Cercopithecidae family, have a significant ecological role, one of which is as a disperser of seeds from the fruits they consume. M. fascicularis is tolerant to different types of natural and non-natural food (human food waste and rubbish), so it is currently an omnivorous animal. It can be found not only in primary and secondary forests but also in habitats close to human activity. M. fascicularis in Bali can be found in sacred areas such as temples, one of which is in the Pulaki temple area. This research aimed to determine the variety of food choices of M. fascicularis in the Pulaki temple area. This research was conducted from June to October 2023. M. fascicularis in the Pulaki Temple area was divided into four groups: Madya, Medal, Pabean, and Parkiran. The daily activities of M. fascicularis observed in this study were sleeping, grooming, sexual activity, moving, eating, caring for children, playing, and agonistic (violent activity). Based on calculations, the consumption of non-natural foods had a higher frequency (87.18%) than natural foods (12.78%). During the research, M. fascicularis in the Pura Pulaki area had the highest preference for Gemitir flowers (n=2299). Gemitir flowers are one of the offerings fillings by Hindus who perform prayers. The high consumption of non-natural food by M. fascicularis in this area was due to several factors, namely, the lack of natural food sources and the low level of human awareness for not providing non-natural food, thus changing the animals' natural behavior. This behavior change could alter the role of animals in the ecological niche, thereby reducing the balance of the ecosystem. Therefore, it is necessary to maintain the natural behavior of animals by increasing natural food sources and creating clear rules regarding the prohibition of feeding animals to visitors. 
The Inventory of ferns (Pteridophyta) in East Citorek, Lebak, Banten Nugraheni, Latif Sofiana; Survani, Rifki; Arindri, Dinda
Al-Hayat: Journal of Biology and Applied Biology Vol 7, No 1 (2024)
Publisher : Fakultas Sains dan Teknologi, UIN Walisongo Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21580/ah.v7i1.20902

Abstract

East Citorek Village in Lebak Regency is a village that has a beautiful, cool and lush environment, and there are a lot of springs. These environmental conditions support the growth of various types of ferns. The people of East Citorek Village are not aware of varied types of ferns in their area. With the development plan of East Citorek Village as one of tourist destinations that is in the process of building facilities and accessibility, it is feared that it bears the potential to reduce and annihilate fern vegetation. The purpose of this study was to identify the types of fern (Pteridophyta) and determine the abiotic factors of ferns (Pteridophyta) at East Citorek Village, Lebak Regency. This research uses incorporates a qualitative approach using the cruise method, particularly by observing directly and exploring each place that is considered representative of the fern vegetation that becomes the concern of the study.  Each type of fern plant was sampled to be identified and collected as a dry herbarium. The results of the study found that there were 19 species, 12 families, and 7 orders of fern in the area. The most common type of fern plant found comes from the Polypodiaceae family, while the type of fern that dominates the study site is the species of Dicranopteris linearis. The results of measuring environmental abiotic factors at East Citorek Village in Lebak Regency show that the results are still feasible for the survival of ferns within the average temperature measurement of 28◦C, air humidity of 76.3%, and soil pH of6.Keywords: Identification, Fern, Pteridophyta, Herbarium
Callus Induction from Stem Explants of Binahong (Anredera cordifolia (Ten.) Steenis) with the Addition of Picloram and BAP Aulia, Siska Na'ilil; Habibah, Noor Aini
Al-Hayat: Journal of Biology and Applied Biology Vol 7, No 1 (2024)
Publisher : Fakultas Sains dan Teknologi, UIN Walisongo Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21580/ah.v7i1.17799

Abstract

The many benefits of medicinal plants require development techniques that aim to produce high and diverse secondary metabolite contents. The purpose of this study was to analyze the effect of the concentration of picloram and BAP and determine the optimal concentration in inducing binahong callus. The research design used was a completely randomized design with two factors, namely the combination of picloram and BAP (0, 1, 2, and 3 ppm). The incubation process of binahong callus was carried out for 40 days after planting (DAP). The observed callus growth indicators consisted of days to callus formation (days), percentage of callus explants (%), callus morphology (texture and color), and callus diameter. Data on days to callus formation and frequency of callus explants were analyzed using the Kruskal-Wallis test and further tested by Dunn if there was a significant effect. The results showed that picloram significantly affected the parameters of days to callus formation and the percentage of callus explants, while BAP significantly affected the parameters of days to callus formation and did not significantly affect the percentage of callus explants parameter. At concentrations of 1 ppm, 2 ppm, and 3 ppm, picloram had a significant effect on both the days to callus formation and the percentage of callus explants parameters. BAP at concentrations of 1 ppm, 2 ppm, and 3 ppm only had a significant effect on the parameter of days to callus formation appearance and was not significant in the percentage of callus explants parameter. The interaction between BAP and picloram had a significant effect on the parameters of days to callus formation and the percentage of callus explants. The resulting callus has a compact texture with callus colors ranging from white, and green, to brown. The most optimal concentration in all indicators is 0 ppm BAP + 1 ppm picloram with days to callus formation of 6 DAP, 100% explants form callus, overall callus is white with compact texture, and has a very high callus growth.
Optimizing Gram-Negative Bacteria Isolation from Children Diarrheal Stool Samples in Jakarta: A Comparative Analysis of Six Culture Media Abdelrazig, Omnia Amir Osman; Fadilah, Fadilah; Prasetyo, Dimas Seto; Saharman, Yulia Rosa; Hegar, Badriul
Al-Hayat: Journal of Biology and Applied Biology Vol 7, No 1 (2024)
Publisher : Fakultas Sains dan Teknologi, UIN Walisongo Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21580/ah.v7i1.20528

Abstract

The prevalence of diarrheal illnesses in Indonesia remains a significant concern among children under the age of five, evident through diagnostic findings and symptomatic manifestations, leading to increased rates of mortality and morbidity. This study aimed to identify, isolate, and culture gram-negative bacteria responsible for childhood diarrhea. Twenty stool samples were collected from children under the age of 5 with acute diarrhea in Jakarta health centres and hospitals between October 2023 to January 2024. These samples were inoculated onto selective agars to facilitate the growth of gram-negative bacteria. After a 24-hour incubation period, colonies suspected to be E. coli, K. pneumoniae, Salmonella, Shigella, or Vibrio were identified using the Vitek-2 compact system. The results revealed a notable presence of Gram-negative bacteria in all the fecal samples from diarrheal children, with Escherichia coli (E. Coli) and Klebsiella pneumoniae (Klebsiella p) as the predominant species. In conclusion, our study, utilizing various cultures and the Vitek-2 compact system, elucidates the microbial landscape driving diarrheal morbidity in Indonesian children under five, with E. Coli and klebsiella pneumoniae emerging as prevalent contributors to childhood diarrhea in this population.

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