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AL-HAYAT: Journal of Biology and Applied Biology
Published by Universitas Islam Negeri Walisongo Semarang
ISSN : 26543702     EISSN : 26220725     DOI : -
Core Subject : Science,
Biochemistry, Genetics & Molecular Biology
AL-HAYAT: Journal of Biology and Applied Biology an international journal focuses on biological science, biotechnology, bioremidiation, environmental biology, and biological education. It is intended to communicate original research and article review in current issues on the subject. The subject covers field studies and literary with various perspectives, both interdiscipline and multidiscipline, i.e. ecology, technology, education and much more. This journal is published by Fakultas Sains dan Teknologi, Universitas Islam Negeri (UIN) Walisongo Semarang Indonesia, and managed to be issued twice in every volume. We warmly welcome contributions from scholars and researchers of many disciplines.
Arjuna Subject : -
Articles 257 Documents
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Bioconcentration and Bioaccumulation 0f Chromium Heavy Metal in Small-Sclae Catfish Farming Ponds Augita Tri Clara Shostakovich Datu Rara
Al-Hayat: Journal of Biology and Applied Biology Vol 6, No 1 (2023)
Publisher : Fakultas Sains dan Teknologi, UIN Walisongo Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21580/ah.v6i1.14615

Abstract

Pollution of the Opak River by heavy metals such as chromium can have an impact on the use of river water for aquaculture in the Bantul Regency area. This study aims to determine the level of concentration of chromium contamination in water samples, sediments and their accumulation in catfish cultivated by the community. The method in this study in taking samples of sediment, water, and catfish is in the form of a cluster random sampling method. Chromium level analysis using AAS (Atomic Adsorption Spectrophotometer) instrumentation-based tests. The results of this reaseacrh show that chromium pollutants from the Opak river have been dispersed into fish farming ponds through irrigation canals. Chromium concentrations in water, sediments, and catfish with ranges and averages respectively of 0.113-0.144 mg/L, 0.667-1.281 mg/L, and 0.258-0.434 mg/L.
Implementation of Zoo Management Strategies for Animal Welfare in Indonesia Risqi Mutia Arba; Wilson Novarino; Rizaldi Rizaldi
Al-Hayat: Journal of Biology and Applied Biology Vol 6, No 1 (2023)
Publisher : Fakultas Sains dan Teknologi, UIN Walisongo Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21580/ah.v6i1.16261

Abstract

A zoo is a place to live or collect wild animals outside their natural habitat which can be controlled by the government or non-government, and functions as a breeding ground so that natural genetics is maintained. Apart from being a place of conservation, it is also a place of education and a place of recreation. The problem with zoos in Indonesia is that their management is not by the principles of animal welfare. This study aims to summarize the results of the Systematic Literature Review (SLR) by collecting reference articles related to the keyword "Implementation of Zoo Management Strategies based on Animal Welfare Standards". Management efforts must pay attention to the survival of animals by the principles of animal welfare, namely freedom from hunger and thirst; free from pain, injury, and disease; free from environmental discomfort, abuse, and violence; freedom from fear and depression; and free in expressing natural behavior. Pay attention to zoo management, both animals and officers or keepers according to their expertise. Strategy implementation using SWOT analysis, namely analysis that obtains useful strategies to implement or recommendations based on results that can maximize Strengths and Opportunities, by minimizing Weaknesses and avoiding Threats.
The Effect of the Combination of Black Soybean Tempeh Extract (Glycine max (L.) Merril) and Purple Sweet Potatoes (morning glory potatoesL.) on Leptin Hormone Levels in DMT2 Model Rats Atika Anggraini; Jevi Milda Rahmawati; Sri Rahayu Lestari; Abdul Gofur
Al-Hayat: Journal of Biology and Applied Biology Vol 6, No 1 (2023)
Publisher : Fakultas Sains dan Teknologi, UIN Walisongo Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21580/ah.v6i1.12930

Abstract

Diabetes mellitus (DM) is a metabolic disorder characterized by chronic hyperglycemia. The prevalence of DM has been reported to increase from year to year. T2DM is characterized by insulin resistance, Leptin have an effect on insulin secretion, glucose and insulin will decrease due to leptin secretion. However, low levels of leptin that exceed the normal limit will interfere with insulin work so that it triggers diabetes caused by insulin resistance. Black soybean tempeh and purple sweet potato have therapeutic potential for people with T2DM. Both contain high antioxidants This study aims to determine the effect of the combination of black soybean tempeh extract and purple sweet potato on leptin hormone levels in DMT2 rat model. The experimental animals in this study were male white rats of the wistar strain which were divided into 6 groups. Preparation of hyperglycemic rats by feeding high-fat diet for 30 days, 10% sucrose drink for 30 days and streptozotocin injection at a dose of 35 mg/kg BW in 0.1 citrate buffered saline pH 4.5. Treatment using extract and purple sweet potato for 30 days. Mice were dissected and blood serum was taken for analysis of leptin hormone levels. Based on the results of the research data, there was an effect of the combination of black soybean tempeh extract and purple sweet potato on the leptin hormone levels in DMT2 rat model.
Hispathology Overview Uterus of the Mice (Mus musculus) ICR Female after Giving Extract of Papaya Seed (Carica papaya) Zulkarnain zulkarnain; Nur Maghfirah Qadriati Putri; St. Aisyah Sijid
Al-Hayat: Journal of Biology and Applied Biology Vol 6, No 1 (2023)
Publisher : Fakultas Sains dan Teknologi, UIN Walisongo Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21580/ah.v6i1.14946

Abstract

The high maternal mortality rate (MMR) and infant mortality rate (IMR) are the main cases in the health sector. Efforts to prevent this are encouraged to utilize plants that contain antifertility compounds, for example with papaya seeds (Carica papaya) which are able to minimize the side effects of contraceptive use. This study aims to determine the description of uterine histopathology of female ICR mice (Mus musculus) after administration of papaya seed extract (Carica papaya). The stages are determining the dose of papaya seed extract (Carica papaya), making papaya seed extract (Carica papaya), preparation and treatment of experimental animals, as well as making and reading uterine histopathology preparations of mice. The results showed that the administration of papaya seed extract (Carica papaya) for 34 days orally at a dose of 0.5 ml/bb was able to disrupt reproductive cells in mice (Mus musculus) characterized by abnormal endometrial thickening along with the amount of dose given.
Study of Ethnobotany in Ginger (Zingiber officinale Rosc.) in Tamiang Village, Banten Gut Windarsih; Asih Perwita Dewi; Deni Mulyana; Arif Gozali Warso Saputro; Muhammad Efendi
Al-Hayat: Journal of Biology and Applied Biology Vol 6, No 1 (2023)
Publisher : Fakultas Sains dan Teknologi, UIN Walisongo Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21580/ah.v6i1.15986

Abstract

Ginger (Zingiber officinale Rosc.) is one of plant species widely used as traditional medicine.The aim of the study was to determine the utilization and ethnobotany of ginger for local communities in Tamiang village, Serang district, Banten. The type of research was descriptive. The research method was the exploratory survei and the participatory rural appraisal. The data collection techniques were conducted through interview, questionnaire, field observation, and documentation. The respondents were determined using purposive sampling and snowball sampling methods. Based on the results of research, the three local varieties of ginger used by local communities in Tamiang village, namely emprit, elephant, and red gingers. The utilization of ginger by local communities in that village was for food and drink ingredients, spices, medicinal ingredients, and ornamental plant. A total of 34.21% of respondents had consumed red ginger to increase the immune system to prevent the Covid-19 virus infection during the pandemic period.
Community Structure and Macroalgae Diversity in Peh Pulo Beach, Blitar Regency Marinda Sari Sofiyana; Mar’atus Sholihah; Dewi Setyaningsih
Al-Hayat: Journal of Biology and Applied Biology Vol 6, No 2 (2023)
Publisher : Fakultas Sains dan Teknologi, UIN Walisongo Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21580/ah.v6i2.17767

Abstract

The coastal area of Peh Pulo Beach, located in the Blitar Regency, has a high potential for biodiversity, including macroalgae. This research aims to understand the community structure and diversity of macroalgae at Peh Pulo Beach. The sampling was conducted on August 2023 using the 1 x 1 m quadrant transect method. Macroalgae were collected at three observation stations, with three plots observed at each station. Abiotic environmental parameters measured included temperature, salinity, pH, light intensity, and substrate. The results of macroalgae identification at Peh Pulo Beach reveal 21 species belonging to 16 genera and 14 families. These species were classified into Chlorophyta (9 species), Phaeophyta (4 species), and Rhodophyta (8 species). The Shannon Diversity Index value obtained is 2.84, indicating that the macroalgae diversity along the coast of Peh Pulo falls under the moderate category.
Description of polimorfism FTO Gene (Fat and Mass Obesity associated) rs9939609 on Obesity and Non Obesity Subject Fitria Diniah Janah Sayekti; Alfian Marfianto
Al-Hayat: Journal of Biology and Applied Biology Vol 6, No 2 (2023)
Publisher : Fakultas Sains dan Teknologi, UIN Walisongo Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21580/ah.v6i2.16547

Abstract

Obesity is a major risk factor for several chronic diseases and is a major health and economic burden on society. The FTO gene (Fat and Mass Obesity associated) was identified as a gene that has an influence on the incidence of obesity according to Genome-wide Association Studies. Examination of the FTO gene polymorphism rs9939609 using 15 samples of obese subjects and 15 samples of non-obese subjects at Prodia Pusat Jakarta employees. This study used descriptive research to describe the polymorphism or variation of the FTO (Fat mass and obesity-associated) gene rs9939609 in obese and non-obese objects among Prodia Pusat Jakarta employees. In this study, non-obese subjects obtained 100% AT allele results. This indicates that genetically there is a potential risk factor for obesity. In obese subjects, 60% of the TT alleles were found. Genetically it should not be a risk allele for the risk of obesity. This can be caused by lifestyle or diet. In this study, an AA allele of 6.67% was found, indicating a greater risk of obesity. From the study, it was concluded that the percentage of the FTO gene polymorphism rs9939609 in obese and non-obese subjects in Prodia Pusat Jakarta employees was the AT allele 100% for obese subjects and for obese subjects. non-obese subject of 33.33%. The AA allele is 6.67% for obese subjects, and the TT allele is 60% for obese subjects.
Deteksi Bakteri Endofit Dan Pengaruh Microbacterium arborescens Terhadap Pertumbuhan Sorgum Manis (Sorghum bicolor) Riana Nindita Putri
Al-Hayat: Journal of Biology and Applied Biology Vol 4, No 2 (2021)
Publisher : Fakultas Sains dan Teknologi, UIN Walisongo Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21580/ah.v4i2.18820

Abstract

This research aims to detect the presence of potential endophytic bacteria, one of which is Microbacterium arborescens and study the effects on the growth of local variants of sweet sorghum plants from farmers in Sleman, Yogyakarta. This research began with infection of Microbacterium arborescens bacteria on sorghum seeds. Sorghum plants were grown in sterile media for 14 days after germination to observe seven growth parameters, including root length, number of lateral roots, stem length, number of leaves, leaf length, wet weight, and dry weight. Analysis of sweet sorghum growth parameters based on the one way ANOVA statistical test. Samples of infected and uninfected sorghum plants in the roots, stems, and leaves were amplified for the 16s rRNA gene using Bioline PCR hotstart mix. The primers used are universal primer Primer 27 f (59-AGA GTT TGA TCM TGG CTC AG-39) and 1492 r (59- TAC GGH TAC CTT GTT ACG ACT T-39). The root length and number of lateral roots between sweet sorghum with and without Microbacterium arborescens infection showed significant differences. The length of the control roots was 9.24 cm, the treatment plants were 6.38 cm, the control plants had six treatment roots, and the treatment plants had ten lateral roots.
Ethnobotani of Suweg (Amorphophallus Paeoniifolius (Dennst.) Nicolson) in Darupono Village, Kendal District Putri Cahya Dinalhaq; Baiq Farhatul Wahidah; Miswari Miswari
Al-Hayat: Journal of Biology and Applied Biology Vol 6, No 2 (2023)
Publisher : Fakultas Sains dan Teknologi, UIN Walisongo Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21580/ah.v6i2.18573

Abstract

Suweg (Amorphophallus paeoniifolius (Dennst.) Nicolson) merupakan tumbuhan liar yang tumbuh dan dimanfaatkan oleh masyarakat desa Darupono. Penelitian ini bertujuan untuk mengetahui pemanfaatan tanaman suweg di Desa Darupono, organ tanaman suweg apa saja yang dimanfaatkan, dan potensi tanaman suweg, serta mengetahui cara budidaya tanaman suweg di Desa Darupono. Penelitian ini tergolong penelitian deskriptif eksploratif dengan menggunakan metode survei, wawancara semi terstruktur, dan menggunakan data pendukung yaitu kuesioner. Pengumpulan data dilakukan dengan teknik acak sederhana dan snowball sampling. Penelitian yang dilakukan di Desa Darupono menunjukkan bahwa tanaman suweg memiliki beberapa potensi antara lain mencegah diabetes, antibakteri, dan antikanker, serta dapat diolah menjadi tepung suweg dan bahan dasar pembuatan mie. Tanaman suweg dimanfaatkan oleh masyarakat Desa Darupono sebagai bahan pangan yaitu dengan cara dikukus, direbus, digoreng, dan diolah menjadi emping, com, pote dan sayur daun oblok-oblok suweg . Selain itu, suweg juga digunakan untuk upacara adat tujuh bulanan atau dalam bahasa Jawa disebut mitoni. Masyarakat Desa Darupono membudidayakan suweg dengan menanam umbi-umbian. Tahapan budidaya suweg adalah penyiapan lahan, penyiapan benih, penanaman, pemeliharaan dan pemanenan.
Authentication of Wax Apple (Syzygium samarangense (Blume) Merr. & L.M Perry) Delima and Citra Cultivars by Morphological and Molecular Approach Arnia Sari Mukaromah; Malia Ulfah; Annisa Nur Rachmah; Muhammad Ramdhani Arfan; Niken Kusumarini; Asri Febriana
Al-Hayat: Journal of Biology and Applied Biology Vol 6, No 1 (2023)
Publisher : Fakultas Sains dan Teknologi, UIN Walisongo Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21580/ah.v6i1.18713

Abstract

Wax apple Delima and Citra Cultivars are two superior non-climacteric tropical fruit commodities from Demak Regency which have similar morphological characters but have different fruit characteristics. Identification wax apple cultivars from Demak regency using DNA barcoding approach has not been researched yet.  The aims of this research are to analyze the morphological similarities of wax apple between Delima and Citra cultivars and to identify genetic variations of wax apple Delima and Citra using trnL-trnF intergenic spacer for molecularly authentication. The results shown that there were no differences in the environmental parameters of the wax apple two cultivated area in Demak Regency. According to the morphological approach, the Delima and Citra cultivars in Demak Regency were grouped separately into Delima and Citra clusters with a similarity index of 61.5% (Citra cultivars) and 60.5% (Delima cultivars) and separated based on the origin of their cultivation area. Genetic variations between wax apple Delima and Citra in Demak Regency consisted of deletions (9delA 17delA) and conserved P6 loop in all compared cultivars. The possibility of heteroplasmy R (A,G) found at 73 nucleotide number in Delima Betokan cultivar and Citra cultivars (Betokan and Jungpasir). Meanwhile, Delima Jungpasir cultivar is K (G,T). There were P8 stem-loops with different lengths between Delima cultivars (Betokan and Jungpasir) and the same length in Citra cultivars (Betokan and Jungpasir). Therefore, the trnL-trnF intergenic spacer has not been thoroughly used in wax apple Delima and Citra authentication due to the presence of a secondary structure which causes the loss of the electropherogram signal so that the nucleotide sequence cannot be read.

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