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AL-HAYAT: Journal of Biology and Applied Biology
Published by Universitas Islam Negeri Walisongo Semarang
ISSN : 26543702     EISSN : 26220725     DOI : -
Core Subject : Science,
Biochemistry, Genetics & Molecular Biology
AL-HAYAT: Journal of Biology and Applied Biology an international journal focuses on biological science, biotechnology, bioremidiation, environmental biology, and biological education. It is intended to communicate original research and article review in current issues on the subject. The subject covers field studies and literary with various perspectives, both interdiscipline and multidiscipline, i.e. ecology, technology, education and much more. This journal is published by Fakultas Sains dan Teknologi, Universitas Islam Negeri (UIN) Walisongo Semarang Indonesia, and managed to be issued twice in every volume. We warmly welcome contributions from scholars and researchers of many disciplines.
Arjuna Subject : -
Articles 257 Documents
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Variations in Food Choices of Long-Tailed Monkeys (Macaca fascicularis) in the Pulaki Temple Area, Gerokgak District, Buleleng Regency Syah, Moh Jafron; Yuliastuti, Yuliastuti; Safitri, Mely
Al-Hayat: Journal of Biology and Applied Biology Vol 7, No 1 (2024)
Publisher : Fakultas Sains dan Teknologi, UIN Walisongo Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21580/ah.v7i1.20674

Abstract

AbstractMacaca fascicularis, included in the Cercopithecidae family, have a significant ecological role, one of which is as a disperser of seeds from the fruits they consume. M. fascicularis is tolerant to different types of natural and non-natural food (human food waste and rubbish), so it is currently an omnivorous animal. It can be found not only in primary and secondary forests but also in habitats close to human activity. M. fascicularis in Bali can be found in sacred areas such as temples, one of which is in the Pulaki temple area. This research aimed to determine the variety of food choices of M. fascicularis in the Pulaki temple area. This research was conducted from June to October 2023. M. fascicularis in the Pulaki Temple area was divided into four groups: Madya, Medal, Pabean, and Parkiran. The daily activities of M. fascicularis observed in this study were sleeping, grooming, sexual activity, moving, eating, caring for children, playing, and agonistic (violent activity). Based on calculations, the consumption of non-natural foods had a higher frequency (87.18%) than natural foods (12.78%). During the research, M. fascicularis in the Pura Pulaki area had the highest preference for Gemitir flowers (n=2299). Gemitir flowers are one of the offerings fillings by Hindus who perform prayers. The high consumption of non-natural food by M. fascicularis in this area was due to several factors, namely, the lack of natural food sources and the low level of human awareness for not providing non-natural food, thus changing the animals' natural behavior. This behavior change could alter the role of animals in the ecological niche, thereby reducing the balance of the ecosystem. Therefore, it is necessary to maintain the natural behavior of animals by increasing natural food sources and creating clear rules regarding the prohibition of feeding animals to visitors. 
The Inventory of ferns (Pteridophyta) in East Citorek, Lebak, Banten Nugraheni, Latif Sofiana; Survani, Rifki; Arindri, Dinda
Al-Hayat: Journal of Biology and Applied Biology Vol 7, No 1 (2024)
Publisher : Fakultas Sains dan Teknologi, UIN Walisongo Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21580/ah.v7i1.20902

Abstract

East Citorek Village in Lebak Regency is a village that has a beautiful, cool and lush environment, and there are a lot of springs. These environmental conditions support the growth of various types of ferns. The people of East Citorek Village are not aware of varied types of ferns in their area. With the development plan of East Citorek Village as one of tourist destinations that is in the process of building facilities and accessibility, it is feared that it bears the potential to reduce and annihilate fern vegetation. The purpose of this study was to identify the types of fern (Pteridophyta) and determine the abiotic factors of ferns (Pteridophyta) at East Citorek Village, Lebak Regency. This research uses incorporates a qualitative approach using the cruise method, particularly by observing directly and exploring each place that is considered representative of the fern vegetation that becomes the concern of the study.  Each type of fern plant was sampled to be identified and collected as a dry herbarium. The results of the study found that there were 19 species, 12 families, and 7 orders of fern in the area. The most common type of fern plant found comes from the Polypodiaceae family, while the type of fern that dominates the study site is the species of Dicranopteris linearis. The results of measuring environmental abiotic factors at East Citorek Village in Lebak Regency show that the results are still feasible for the survival of ferns within the average temperature measurement of 28◦C, air humidity of 76.3%, and soil pH of6.Keywords: Identification, Fern, Pteridophyta, Herbarium
Callus Induction from Stem Explants of Binahong (Anredera cordifolia (Ten.) Steenis) with the Addition of Picloram and BAP Aulia, Siska Na'ilil; Habibah, Noor Aini
Al-Hayat: Journal of Biology and Applied Biology Vol 7, No 1 (2024)
Publisher : Fakultas Sains dan Teknologi, UIN Walisongo Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21580/ah.v7i1.17799

Abstract

The many benefits of medicinal plants require development techniques that aim to produce high and diverse secondary metabolite contents. The purpose of this study was to analyze the effect of the concentration of picloram and BAP and determine the optimal concentration in inducing binahong callus. The research design used was a completely randomized design with two factors, namely the combination of picloram and BAP (0, 1, 2, and 3 ppm). The incubation process of binahong callus was carried out for 40 days after planting (DAP). The observed callus growth indicators consisted of days to callus formation (days), percentage of callus explants (%), callus morphology (texture and color), and callus diameter. Data on days to callus formation and frequency of callus explants were analyzed using the Kruskal-Wallis test and further tested by Dunn if there was a significant effect. The results showed that picloram significantly affected the parameters of days to callus formation and the percentage of callus explants, while BAP significantly affected the parameters of days to callus formation and did not significantly affect the percentage of callus explants parameter. At concentrations of 1 ppm, 2 ppm, and 3 ppm, picloram had a significant effect on both the days to callus formation and the percentage of callus explants parameters. BAP at concentrations of 1 ppm, 2 ppm, and 3 ppm only had a significant effect on the parameter of days to callus formation appearance and was not significant in the percentage of callus explants parameter. The interaction between BAP and picloram had a significant effect on the parameters of days to callus formation and the percentage of callus explants. The resulting callus has a compact texture with callus colors ranging from white, and green, to brown. The most optimal concentration in all indicators is 0 ppm BAP + 1 ppm picloram with days to callus formation of 6 DAP, 100% explants form callus, overall callus is white with compact texture, and has a very high callus growth.
Optimizing Gram-Negative Bacteria Isolation from Children Diarrheal Stool Samples in Jakarta: A Comparative Analysis of Six Culture Media Abdelrazig, Omnia Amir Osman; Fadilah, Fadilah; Prasetyo, Dimas Seto; Saharman, Yulia Rosa; Hegar, Badriul
Al-Hayat: Journal of Biology and Applied Biology Vol 7, No 1 (2024)
Publisher : Fakultas Sains dan Teknologi, UIN Walisongo Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21580/ah.v7i1.20528

Abstract

The prevalence of diarrheal illnesses in Indonesia remains a significant concern among children under the age of five, evident through diagnostic findings and symptomatic manifestations, leading to increased rates of mortality and morbidity. This study aimed to identify, isolate, and culture gram-negative bacteria responsible for childhood diarrhea. Twenty stool samples were collected from children under the age of 5 with acute diarrhea in Jakarta health centres and hospitals between October 2023 to January 2024. These samples were inoculated onto selective agars to facilitate the growth of gram-negative bacteria. After a 24-hour incubation period, colonies suspected to be E. coli, K. pneumoniae, Salmonella, Shigella, or Vibrio were identified using the Vitek-2 compact system. The results revealed a notable presence of Gram-negative bacteria in all the fecal samples from diarrheal children, with Escherichia coli (E. Coli) and Klebsiella pneumoniae (Klebsiella p) as the predominant species. In conclusion, our study, utilizing various cultures and the Vitek-2 compact system, elucidates the microbial landscape driving diarrheal morbidity in Indonesian children under five, with E. Coli and klebsiella pneumoniae emerging as prevalent contributors to childhood diarrhea in this population.
Antioxidant Activity Of The Peel Citrus sinensis. L On The Histological Features Of Second Degree Burned Mus musculus Samsudin, Rinza Rahmawati; Arimurti, Anindita Riesti Retno; Puspitasari, Renny Novi
Al-Hayat: Journal of Biology and Applied Biology Vol. 7 No. 2 (2024)
Publisher : Fakultas Sains dan Teknologi, UIN Walisongo Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21580/ah.v7i2.17580

Abstract

Burns are damage to the tissue that does not only occur on the surface of the skin, but can occur under the skin. Globally, burns are the fourth most common type of injury, after traffic accidents, falls and physical abuse. The cost of treating burns is relatively expensive according to the area of the burn, the larger the area of the burn, the higher the cost of treatment. Research for the treatment of burns using herbal ingredients has begun to be carried out by many researchers. One of the typical Indonesian herbal plant ingredients is the Pacitan orange (Citrus sinensis) L Osbeck. This type of research is an experimental research with the aim of looking at the formation of epithelial cells, fibroblasts, and collagen which are formed after being given Pacitan orange peel extract (Citrus sinensis) treatment. The sample of this study used 20 white rats which were divided into 4 groups, namely Group 1 (K1) the burn group without treatment, Group 2 (K2) the burn rats with bioplacenton treatment, Group 3 (K3) the burn rats with NaCl treatment 0.9%, Group 4 (K4) burnt rats treated with extra 100% Pacitan orange peel. From the research results obtained, it can be concluded that the administration of Pacitan Orange Peel extract is proven to accelerate the healing process of second degree burns on the skin of white rats viewed microscopically, namely from increased collagen production, epithelial thickness, and fibroblasts.Keywords: Pacitan orange peel extract, Burns, Mus musculus, histopathology
Diversity of Bactrocera spp. in Different Habitat Types in Citrus Orchards at Malang Regency Rahmawati, Yunita Fera; Leksono, Amin Setyo; Gama, Zulfaidah Penata; Rizali, Akhmad
Al-Hayat: Journal of Biology and Applied Biology Vol. 7 No. 2 (2024)
Publisher : Fakultas Sains dan Teknologi, UIN Walisongo Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21580/ah.v7i2.20834

Abstract

Fruit flies are important pests of citrus crops. Identifying fruit fly species is very important to make the appropriate conclusions in determining controlling methods. This study was intended to establish the dominant species, diversity index, and identification of fruit fly species in Siam citrus (Citrus nobilis Lour.) orchards in Dau District, Malang Regency, Indonesia. This study was conducted in two habitat types: close and far from residential areas. Six petrogenol-treated traps were placed at each location from December 2022 to February 2023, and every two weeks, the fruit flies caught in the traps were counted as part of the survey. The results showed that three species, namely B. dorsalis, B. carambolae, and B. umbrosa, were found in locations close to residential areas, while B. umbrosa was not found in locations far from residential areas. The dominant species was B. dorsalis in both locations. The diversity index of Bactrocera spp. in orchards close to residential areas was 1.14 (moderate), and Spearman's analysis (0.913) showed a more substantial influence than in orchards far from residential areas. Climatic factors of air humidity, air pressure, and host availability play an essential role in the presence of fruit flies.
Detection of NFKB1 Gene in Patients with Type 2 Diabetes Mellitus and Non Diabetics Using Polymerase Chain Reaction Method Cahyaningtyas, Priska Putri; Sayekti, Fitria Diniah Janah
Al-Hayat: Journal of Biology and Applied Biology Vol. 7 No. 2 (2024)
Publisher : Fakultas Sains dan Teknologi, UIN Walisongo Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21580/ah.v7i2.20856

Abstract

A metabolic condition called type 2 diabetes mellitus is characterized by elevated blood sugar levels because of reduced insulin release by β pancreatic cells. The primary form of NF-kB is Nuclear Factor Kappa Beta subunit-1 (NFKB1), which is a gene that encodes the DNA binding protein (p50). The NFKB1 gene contributes to the oxidative stress and mild inflammatory processes that might exacerbate diabetes. The enzymatic procedure known as Polymerase Chain Reaction (PCR), multiplies a nucleotide sequence in order to identify if type 2 diabetes mellitus or non-diabetic has the NFKB1 gene. The purpose of this study was to detect the presence of the NFKB1 gene in type 2 diabetes mellitus and non-diabetes. The study used a descriptive research method using a purposive sampling technique conducted at the molecular biology laboratory of the Sekolah Tinggi Ilmu Kesehatan Nasional. The respondents in this study were 9 patients with type 2 diabetes mellitus who were prolanes of Puskesmas Wonosari I Klaten and 9 non-diabetic patients in PKK Kadilangu RT 2/RW 1, Baki, Sukoharjo. From the results of the study, it can be seen that the NFKB1 gene was detected after electrophoresis and visualized at 176 bp (base pair). The qualitative presence of the NFKB1 gene in DNA is still detectable, but the level of gene expression in the soil of transcription and translation is not yet known.
Appropriate Concentration of Curcumin as a Growth Factor in Neural Stem Cells Novianti, Titta; Kurniawan, Muhamad Panji Januarsyah; Nainggolan, Ita M
Al-Hayat: Journal of Biology and Applied Biology Vol. 7 No. 2 (2024)
Publisher : Fakultas Sains dan Teknologi, UIN Walisongo Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21580/ah.v7i2.20862

Abstract

The growth of Neural Stem Cell (NSC) in adult organisms is limited. Therefore, growth factors are needed to stimulate NSC cell proliferation and differentiation. Herbal Curcumin may be a growth factor. We promoted the growth of Cryopreserved Rat Cortical NSC cells with Curcumin (0.1 µM; 0.5 µM; 1 µM; 2 µM), DMSO, and synthetic growth factors (bFGF, TGF, and heparin). We analyzed the proliferation ability of NSCs by WST-1 assay, cell morphology, and expression of NCS cell marker genes (Nestin, MAP, and Sox2). Morphological analysis showed that cells reproduced optimally at 0.5 µM. The one-way ANOVA and Tukey's posthoc test on the WST-1 test showed significant differences between 0.5 µMCurcumin and other treatment groups. Sox2, MAP-2, and Nestin gene expression peaked at 0.5 µM. The appropriate concentration of Curcumin to stimulate NSC proliferation is 0.5 µM. Herbal extract curcumin has the same effect as commercial growth factors and can substitute synthetic growth factors. Curcumin acts as a growth factor that stimulates the proliferation of mouse NSCs.
Composition and Distribution of Invasive Species in The Peson Subah I Nature Reserve, Batang District, Central Java santoso, budi; Kurniayu, Tiara Putri
Al-Hayat: Journal of Biology and Applied Biology Vol. 7 No. 2 (2024)
Publisher : Fakultas Sains dan Teknologi, UIN Walisongo Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21580/ah.v7i2.20963

Abstract

Research has been carried out regarding the composition of invasive plants in the Peson Subah I Nature Reserve (PSINR), a conservation area located at Kuripan Village, Subah District, Batang Regency Central Java. The research, which was carried out from 17 to 20 April 2023, aims to identify invasive plants in PSINR and mapping their spatial distribution. This research used a double plot method which was determined purposively with a sampling intensity of 10%. The analysis method used is quantitative descriptive based on the parameters of the vegetation analysis results. Based on the results of observations, 80 plant species were found, of which 53% or 43 species were invasive species. The highest Importance value Index for the invasive species at each tree level is Acacia (Acacia auriculiformis) 66.44, at the pole level it is Klampis (Flacourtia indica) with an Importance value index of 91.46, at the sapling level it is Lampeni (Ardisia elliptica) and at the seedling level the invasive species Lampeni (Ardisia elliptica) 55.16. The plant diversity index in PSINR at each growth level is in the medium category, while the evenness index is in the high to medium category.
Dimensional Stability Test of Mahogany Wood by Heat Treatment Kusumawardhani, Dina Tiara
Al-Hayat: Journal of Biology and Applied Biology Vol. 7 No. 2 (2024)
Publisher : Fakultas Sains dan Teknologi, UIN Walisongo Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21580/ah.v7i2.22225

Abstract

Mahogany wood (Swietenia macrophylla) is one of Indonesia's fast-growing commercial tree species. Its wood is widely used for construction because of its great potential in the timber industry. Mahogany wood has low dimensional stability and high hygroscopic properties. This study aims to improve the quality of mahogany wood in terms of dimensional stability with the heat treatment method. Samples of mahogany wood were heat treated with three-time levels, namely control, 3 hours, and 6 hours at 180 °C. The results showed that heat treatment at 180 °C for 6 hours had the best results for maintaining wood dimensions with a low increase in water content, increased specific gravity, low absorption ability, minor swelling of wood in three directions of wood, and the value of ASE is also common than 3 hours of treatment.

Page 15 of 26 | Total Record : 257

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