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Scripta Biologica

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Published by Universitas Jenderal Soedirman

ISSN : - EISSN : 23553138 DOI : -

Core Subject : Science, Agriculture,

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

Scripta Biologica is a peer reviewed open access electronic journal published by the Faculty of Biology Jenderal Soedirman University, publishes research papers encompasses all aspects of biology, including; molecular biology, cell biology, and genetics; physiology and reproduction; ecology and conservation; biodiversity, evolution, taxonomy, and biogeography.

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Articles 21 Documents

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Search results for , issue "Vol 1, No 1 (2014)" : 21 Documents clear

KAJIAN TOKSISITAS DARI TUBUH BUAH Ganoderma lucidum DENGAN METODE BRINE SHRIMP LETHALITY TEST (BST) Ross Nurul Rohmah; Nuniek Ina Ratnaningtyas; Ari Asnani
Scripta Biologica Vol 1, No 1 (2014)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Ganoderma lucidum is polyporus fungi from Basidiomycetes which can be used as traditional medicines. Toxicity test with Brine Shrimp Lethality Test (BST) method using Artemia salina was conducted to find out toxic effect of G.lucidum. An extract would have toxic effect if the LC50 < 1000 µg/ml. The aims of this experiment were to know about the toxicity level from G.lucidum with Brine Shrimp Lethality Test (BST) method and determined the concentration of fruiting body extract of G.lucidum which had the best toxicity effect in LC50. There were two extraction methods used in this experiment, first one stage extraction and then multilevel extraction with hexane, ethyl acetate, and ethanol. Each solvent was made in 1000 ppm, 500 ppm, 250 ppm, and 125 ppm. This process was repeated for three times. This experiment used G.lucidum extract which was tested to A.salina and secunder compound metabolit test from the most toxic G. lucidum was done with phytochemical analysis. The result indicated that fruiting body extract from G.lucidum could kill A.salina because all extract was positively contained alkaloid and terpenoid but negatively contained flavonoid. Fruiting body extract from G.lucidum which had lowest LC50 wass ethyl asetat extract with one stage extraction in concentration 53,70 ppm and highest LC50 was ethanol extract with multilevel extraction in concentration 501,18 ppm.

ANALISIS RAPD KECIPIR POLONG PANJANG Psophocarpus tetragonolobus (L.) DC HASIL MUTASI IRADIASI SINAR GAMMA Arini Maesaroh; Adi Amurwanto; Alice Yuniaty
Scripta Biologica Vol 1, No 1 (2014)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Winged bean [Psophocarpus tetragonolobus (L.) DC] is a tropical plant that has some benefits and is very suitable to be cultivated in Indonesia. Study about diversity of winged bean is very important to support the future development of winged bean. Increased genetic diversity can be done through mutation. Gamma ray is often used for inducting mutations. One of way to observe genetic diversity resulted by molecular mutation is using RAPD method. The purpose of this study was to identify the genetic diversity of winged bean resulted by gamma ray irradiation. The method used in this study was an experiment by using RAPD technique. Samples used were plant leaves that had been induced by gamma ray with a wavelength of 20 Gy, 25 Gy, and control plants that were not induced by gamma ray. RAPD were done by ten primers that were OPA 9, OPA 10, OPA 13, OPA 18, OPB 2, 3 OPB, OPB 6, 7 OPB, OPB 10, and OPB 11. Data were analyzed using GenAlex 6.1 Program. The percentage of polymorphic loci of winged bean population control was 47,54%, while in the 20 and 25 Gy treatment were 62,30% and 54,10%, respectively. The values of genetic variation based on the calculation of allele frequencies were 0,236; 0,202 and 0,194 for treatment of 20 Gy, 25 Gy and for control plants, respectively. Meanwhile, the value of genetic distance ranged from 0.08 to 0.32.

KEMAMPUAN TANAMAN GLODOGAN Polyalthia longifolia SONN. SEBAGAI PENEDUH JALAN DALAM MENGAKUMULASI Pb UDARA BERDASARKAN RESPON ANATOMIS DAUN DI PURWOKERTO Rizqi Dwi Ardyanto; Slamet Santoso; Siti Samiyarsih
Scripta Biologica Vol 1, No 1 (2014)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Polyalthia longifolia is a plant that used as a roadside in Purwokerto. This study aimed to determine the ability of the plant P. longifolia in the air and accumulate Pb anatomic response to the Pb concentration in the leaves of P. longifolia. The methods of research used survey methods. Roadside plant leaf samples were taken by purposived sampling at six locationsmain street in Purwokerto, there are Jenderal Soedirman, Dr. Soeparno, HR. Bunyamin, Commisioner Bambang Soeprapto and Gerilya. The independent variable was the ambient air Pb and Pb leaf was the dependent variable. The main parameters investigated were air Pb concentrations, Pb leaves, stomata anatomical characters and thick mesophyll tissue. Parameter support was the traffic density. The results showed that the highest concentration of Pb in the air with a concentration of 0.1020 μg/m3 per hour on Gerilya street and the lowest in DR. Soeparno street with concentrations of 0.0048 μg/m3 per hour, it can be concluded that an ambient air Pb in six study sites are still under ambient air quality in Central Java, which is 2 μg/m3 per 24 hours. Leaf Pb concentration was highest in HR. Bunyamin street with a concentration of 0.4980 ± 0.004 ppm and lows in the DR. Soeparno street with concentrations of 0.2786 ± 0.008 ppm. Pb accumulated in leaves affects the density, length, and width of stomata and mesophyll tissue thickness P. longifolia. Pb concentrations was not correlate with the leaf Pb concentration P. longifolia. Pb concentrations of leaves of P. longifolia is closely linked with decreasing density of stomata and mesophyll tissue thickness but not related to the length and width of the stomata.

PENGARUH PEMBERIAN MIKROBA EFEKTIF PRODUKTIF PLUS (MEP+) PADA MEDIUM BUDIDAYA IKAN NILA YANG DIBERI PAKAN FERMENTATIF TERHADAP KEPADATAN BAKTERI ASAM LAKTAT Nita Wulandari; Sukanto Sukanto; Endang Widyastuti
Scripta Biologica Vol 1, No 1 (2014)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Microbes Effective Productive Plus (MEP+) in fish culture has role as probiotics and decomposer. Application of MEP+ is done by adding MEP+ on culture medium of Tilapia and fish feed. Fish feed is fermentative feed with addition of different concentration of cassava peel flour. The aim of this research were to find out the influence of MEP+ administration in culture medium and in fermentative feed with addition cassava peel flour on the increasing density of lactic acid bacteria in culture medium and find out the highest density of lactic acid bacteria. The research was done experimentally, used Complete Randomized Design with treatment of MEP+ administration in culture medium with fermentative feed cassava peel flour addition of 25%, 50%, 75% and without MEP+ administration on culture medium with fermentative feed cassava peel flour addition of 25%. The data obtained were analyzed using a variety analysis. The result showed that MEP+ administration on culture medium and in fermentative feed cassava peel flour addition did not influence the increasing density of lactic acid bacteria and total density of lactic acid bacteria in culture medium was not different inter treatment.

HUBUNGAN PERBANDINGAN TOTAL NITROGEN DAN TOTAL FOSFOR DENGAN KELIMPAHAN CHRYSOPHYTA DI PERAIRAN WADUK PANGLIMA BESAR SOEDIRMAN, BANJARNEGARA Faralenggi DM Putri; Endang Widyastuti; Christiani Christiani
Scripta Biologica Vol 1, No 1 (2014)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Nitrogen (N) and phosphorus (P) affect the growth of phytoplankton in the waters. Nitrogen is an important part in forming protein in organisms, while phosphorus is the most essential element for growth and contributes in protein formation and cell metabolism in organisms. The activities from the catchment area that enter into the P.B Soedirman Reservoir can affect both concentration of N and P. The comparison total nitrogen (TN) and total phosphorus (TP) is the limiting factor to affect the growth of phytoplankton. Chrysophyta is one of division from phytoplankton that has function as an important component in the food chain and become as natural feed for fish. This research showed the abundance of Chrysophyta, relations of TN with the abundance of Chrysophyta; TP with the abundance of Chrysophyta; TN/TP with the abundance of Chrysophyta; TN, TP, TN/TP with the abundance Chrysophyta in the P.B Soedirman Reservoir, Banjarnegara Regency. This research used survey method with purposive sampling technique on 7 observation stations with 3 repeating. The relation of TN/TP with the abundance of Chrysophyta were analyzed used correlation regression - correlation analyzis. The results of the research showed that total abundance Chrysophyta was between 339-9570 ind/l, relation between TN/TP with the abundance of Chrysophyta with the degree was weak to very strong correlation. The most affection correlation was shown by of TN, TP, TN/TP collectively with the abundance of Chrysophyta with coefficient value (r) of 0,890 with the strength of the relation of 79,3%. The regression analysis of TN, TP, TN/TP collectively with the abundance of Chrysophyta was shown by the equation Y= – 80987,801 – 8499,931TN + 1,684TP + 406,373TN/TP.

PERKEMBANGAN OOSIT INDUK Osteochilus hasselti C.V. YANG DIBERI HORMON ESTRADIOL-17β DAN PAKAN DENGAN KADAR PROTEIN BERBEDA Andri Prajaka Santo; Untung Susilo; Gratiana E Wijayanti
Scripta Biologica Vol 1, No 1 (2014)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

The availability of fish seed is very important in fish propagation. Good quality of fish seeds were produced by a good brooder which characterized a large number of egg production at spawning. The aims of this research were to evaluate the oocytes development in the hard-lipped Barb (Osteochilus hasselti C.V.) given 17β-estradiol, different percentage of protein in the diet, and their combination. This research was conducted experimentally applying Factorial Completely Randomized Design. The first factor was percentage of protein in the diet consisted of 4 levels namely 25% (P1), 30% (P2), 35% (P3), and 40% (P4), the second factor was dose of 17β-estradiol benzoate (EB) consisted of 3 levels namely 0 µg/kg of body weight (D0), 126 µg/kg of body weight (D1), and 210 µg/kg of body weight (D2), thus there were 12 combinations of treatment with 3 replicates. The results showed that neither of protein proportion or 17β-estradiol affected the proportion of oocytes of any developmental stage (p>0,05) within the first two weeks post spawning. The treatments, however, significantly increased the proportion of oocytes at V3-V5, V6-V7, and post-vitellogenic stages (p<0,05) started at week 4th post spawning. The GSI increased in correlation to the proportions of oocytes at stage ≥ V6 (r=0,701; p<0,01) and the dose of 17β-estradiol (r = 0.357, p <0.05). There was no significant different on GSI amongst the experimental groups. The HSI tend to decrease as the dose of 17β-estradiol increased (r = -0.210, p> 0.05). In conclusion, the percentage of protein in the diet and different doses of 17β-estradiol improved oocyte development of Hard-Lipped Barb (Osteochilus hasselti C.V.).

IDENTIFIKASI BAKTERI PENGOKSIDASI BESI DAN SULFUR BERDASARKAN GEN 16S rRNA DARI LAHAN TAMBANG TIMAH DI BELITUNG Dhewanti Puspitasari; Hendro Pramono; Oedjijono Oedjijono
Scripta Biologica Vol 1, No 1 (2014)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Heavy metals contamination disturb balance and diversity of microorganism in soil. Microorganisms which can able to survive in those conditions are bacteria capable of oxidizing heavy metals. Identification based on 16S rRNA was used to determine characteristics and phylogenetic relationship of bacteria which can oxidize iron and sulphur in tin mining areas. The aim of this research was able to determine the bacterias characteristics isolated from tin mining areas and determine the phylogenetic relation of iron-sulphur oxidizing bacteria on tin mining soil in Belitung based on 16S rRNA sequences. This research was done using descriptive method, including isolation, morphological characterization, and identification based on 16S rRNA sequences. Morphology characterization includes colony and cell morphology through Gram staining. Molecular characterization includes amplification of 16S rRNA gene (Polymerase Chain Reaction/ PCR), electrophoresis amplicon and sequencing. Bacteria identification was done by comparing the 16S rRNA gene sequence in GenBank. The result showed three bacterias were identified by 16S rRNA have a similarity with Bacillus anthracis strain Ames, Bacillus cereus ATCC 14579, Staphylococcus sciuri subsp. Sciuri strains DSM 20345 and Micrococcus luteus NCTC 2665.

MENGISI KESENJANGAN ANTARA JUMLAH HASIL PENELITIAN MAHASISWA DALAM BIDANG BIOLOGI DAN KETERSEDIAAN MEDIA PUBLIKASINYA Romanus Edy Prabowo
Scripta Biologica Vol 1, No 1 (2014)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Berkala ilmiah Scripta Biologica ini dibuat untuk mengisi celah besar kesenjangan antara jumlah hasil penelitian mahasiswa dalam bidang biologi dan ketersediaan media publikasinya. Scripta Biologica tersedia secara online dalam 4 nomer terbitan per volume per tahun, kualitas artikel ilmiahnya tetap dipertahankan dengan kombinasi editorial-review dan peer-review. Scripta Biologica menerapkan lisensi Creative Commons Attribution BY-SA, di bawah lisensi ini hak cipta atas artikel ilmiah yang dipublikasikan tetap ada pada penulis. Penulis memberikan lisensi kepada Scripta Biologica untuk mempublikasikan artikel ilmiahnya dan menyatakan Scripta Biologica sebagai penerbit pertama, sedangkan pembaca dapat menggunakannya secara bebas; mengunduh, mencetak, menggunakan, mengubah, menyebarkan, dan atau menyalin sebagian atau seluruh artikel ilmiah dalam berkala ini, selama identitas penulis, detil sitasi, dan berkala serta penerbit tetap disertakan dan dinyatakan secara jelas. Semoga berkala ilmiah online Scripta Biologica bisa memberikan kontribusi dalam usaha bersama insan akademik untuk meningkatkan akses pada hasil-hasil penelitian, utamanya penelitian mahasiswa dalam bidang biologi, sehingga pengetahuan serta manfaatnya dapat digunakan oleh masyarakat secara lebih luas. Publikasi perdana Scripta Biologica volume 1 nomer 1 ini adalah langkah awal untuk secara berkelanjutan membuat lebih banyak hasil penelitian mahasiswa dalam bidang biologi menjadi terdiseminasi, terdokumentasi, dan tersertifikasi.

KELIMPAHAN CHRYSOPHYTA PADA MEDIA BUDIDAYA IKAN NILA YANG DIBERI PAKAN FERMENTASI DENGAN PENAMBAHAN TEPUNG KULIT UBI KAYU DAN PROBIOTIK Retno Ambarwati; Endang Widyastuti; Dwi Sunu Widyartini
Scripta Biologica Vol 1, No 1 (2014)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Chrysophyta is also known as golden-yellow algae because of the yellow dominant pigment of carotene and xanthophyll. This study aimed to determine species richness and abundance of Chrysophyta in the Tilapia culture media which was fed with the addition of cassava peel flour and probiotic. The method applied in this study was the experimental method with 4 treatments, i.e.: the use of fermented feed with the addition of cassava peel flour (0%, 25%, 50%, and 75%) and probiotic in Tilapia culture media. Each treatment was replicated 4 times. Sampling was carried out 6 times at intervals of 2 weeks. The observed parameters were the main parameters, i.e.: the number of Chrysophyta species and individuals; and supporting parameters, i.e.: water temperature, pH, TDS, TSS, NO3, NO2, BOD5, DO, and total of phosphate. Species richness and abundance of Chrysophyta data were analyzed using cluster analysis based on Bray-Curtis similarity coefficient. The analysis continued with Similarity Percentages (SIMPER) analysis to determine the contribution of species to the similarity index in each group or to dissimilarity index amongst groups. The results showed that the species richness found in the Tilapia media culture which was fed with the addition of cassava peel flour and probiotic consist of 20 species belonging to a class namely Bacillariophyceae. Abundance of Chrysophyta obtained was ranged from 5.160-13.292 individuals/liter. The cluster analysis showed that Chrysophyta amongst treatments have a quite high similarity level (> 50%) was ranged between 65.56% -83.99%. Contributions of species which contribute the highest similarity index were Diatoma vulgare (49.80%), Navicula brachysira (70.50%) and Navicula platystoma (82%).

ANALISIS TAKSOMETRI Anthurium SCHOTT (ARACEAE) Muhammad Falah; Pudji Widodo; Hexa Apriliana Hidayah
Scripta Biologica Vol 1, No 1 (2014)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Anthurium Schott is one of the commercial ornamental plants in Indonesia. This genus has a high morphological variation amongst its species. This study aims to determine the similarity relationship amongst Anthurium based on morphological characters including stems, leaves, and inflorescences. This study was carried out from June to July 2012 in several locations in Purwokerto and Baturraden area, and Balai Benih Tanaman Pangan dan Hortikultura (B2TPH) Banyumas Regency. This study used explorative method with purposive random sampling. The results of the analysis using the UPGMA (Unweighted Pair Group Method with Arithmetic Mean) showed that there were 17 taxa which can be classified into 4 groups. Group I consisted of A. andraeanum “Renoir Pink”, A. andraeanum “Safari”, A. andraeanum “White”, A. andraeanum “Tropical”, A. andraeanum “cv. 1”, A. andraeanum “Amigo”, and A. nymphaeifolium. Group II consisted of A. pedatoradiatum ssp. helleborifolium, A. crystallinum, A. andicola “Kuku Bima” and A. andicola “Red List”. Group III consisted of A. crassinervium “Crispimarginatum” and A. plowmanii. Group IV consisted of A. hookeri “Garuda”, A. hookeri “Red Hookeri”, A. bonplandii ssp. guayanum, and A. jenmanii. Group I was the sister group of Group II, as well as Group III to Group IV. The closest similarity relationship was between A. andraeanum “Renoir Pink” and A. andraeanum “Safari”, whereas the farthest one was between A. bonplandii ssp. guayanum and A. andraeanum “Tropical”.

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