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Scripta Biologica
Published by Universitas Jenderal Soedirman
ISSN : -     EISSN : 23553138     DOI : -
Core Subject : Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology
Scripta Biologica is a peer reviewed open access electronic journal published by the Faculty of Biology Jenderal Soedirman University, publishes research papers encompasses all aspects of biology, including; molecular biology, cell biology, and genetics; physiology and reproduction; ecology and conservation; biodiversity, evolution, taxonomy, and biogeography.
Arjuna Subject : -
Articles 176 Documents
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PERSEPSI PENGUNJUNG TERHADAP BUAYA MUARA (Crocodylus porosus) DAN FASILITAS PEMELIHARAAN DI PENANGKARAN DAN KEBUN BINATANG Vidya Elza Loriza; Mirza Dikari Kusrini; Resti Meilani
Scripta Biologica Vol 6, No 2 (2019)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

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Abstract

Estuarine crocodile often considered as dangerous animal due to continual news of human crocodile conflict cases around the world.  This study aimed to analyze the perception of visitors about estuarine crocodile in captivity and facilities in two captive breeding sites. We conducted field observation and structured interview to 120 respondents in two local zoos: the Indonesia Jaya Crocodile Park (TBIJ or crocodile park)  in Bekasi and Ragunan Zoo (TMR or the zoo) in Jakarta. All visitors have low knowledge about estuarine crocodile, which they obtained mostly from mass media. Although most visitors know that estuarine crocodies are protected however in pratice their don’t have enough empathy to captive crocodiles. Visitors to both areas are local who lives near the vicinity of the park or zoo. Crocodile park visitors mostly came from Bekasi with an average number of visits 1 time and 1-hour length of visit. The reason for their visit were the closeness of the location and to learn crocodiles. The zoo visitors mostly came from Jakarta with an average number of visits more than 3 times and 6 hours length of visits. Respondents visited the zoo because of low cost and recreation. Visitors of the zoo provided  positive review for several aspects in the zoo, while visitors in crocodile park gave positive review only on physical condition of crocodiles.
Several medicinal plants species which need enrichment planting Agus Ismanto; Andianto Andianto; Rossi Margareth Tampubolon
Scripta Biologica Vol 6, No 2 (2019)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

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Abstract

Pulai (Alstoniasp.), Kayumanis/Cinnamon (Cinnamomum burmanii, Cinnamomum iners), Pakanangi/Kisereh (Cinnamomum parthenoxylon/Cinnamomum porrectum), Kulilawang (Cinnamomum halmaherae), Agarwood (Aquilaria sp., Gyrinops versteeghii and Gyrinopsis cumingiana), and Pasakbumi (Eurycoma Longifolia) are some of  medicinal plants species which growing in plantation or natural forest. The exploitation to these species causes their existence to be scarce or almost not known, with the result that is needed a policy to save the existence of medicinal plants species. Industries of herbal medicine which exploit the raw material from nature need to build seedlings and enrichment area. The collaboration between medicinal industries with local people to build medicinal plants species plantation can be developed. This paper informs a field review of some medicinal plants species from several regions during the research activities in 2005 to 2009.
KEANEKARAGAMAN JENIS VEGETASI STRATA POHON DI AREAL HUTAN PERLINDUNGAN KAWASAN BUKIT COGONG Merti Triyanti
Scripta Biologica Vol 6, No 2 (2019)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

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Abstract

Penelitian ini dilatar belakangi oleh belum terdatanya jenis-jenis pohon dan informasi ekologis hutan perlindungan di kawasan Bukit Cogong. Penelitian ini bertujuan untuk  mengetahui Indeks Keanekaragaman (H’) spesies vegetasi strata pohon dan mengetahui faktor lingkungan abiotik di areal hutan perlindungan kawasan Bukit Cogong. Penelitian ini menggunakan metode Point Centered Quarted, yaitu salah satu metode pengukuran yang tidak menggunakan plot atau metode plotless. Penelitian ini di buat 3 area kajian yaitu area kajian A, area kajian B, dan area kajian C. Secara keseluruhan luas area penelitian yaitu 17 ha atau 170.000 m2 yang diambil 20% dari keseluruhan luas areal hutan perlindungan yaitu 85 ha atau 850.000 m2, sehingga luas masing-masing area kajian 56.666m2. Pada masing-masing area kajian diletakkan 10 stand. Pada setiap stand penelitian diletakkan garis transek utama sepanjang 560 m. Berdasarkan hasil penelitian diperoleh rerata di area kajian A sebesar 1,38, area kajian B sebesar 1,14 dan area kajian C sebesar 1,36. Rerata Indeks Keanekaragaman jenis vegetasi strata pohon di areal hutan perlindungan kawasan Bukit Cogong secara keseluruhan tergolong sedang melimpah dengan kriteria H’ ≤1≤ H’ 3 dan faktor lingkungan abiotik yang terukur di area kajian A, B dan C pada areal hutan perlindungan kawasan Bukit Cogong hasilnya tidak berbeda jauh.
POLLEN STORAGE AND VIABILITY OF TWELVE CITRUS GENOTYPES IN BALI, INDONESIA Ida Ayu Astarini
Scripta Biologica Vol 6, No 3 (2019)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

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Abstract

The aim of this research was to obtain a method for pollen storage of Citrus species in Bali. Pollen from 12 Citrus genotypes was collected from five different regions; Petang, Plaga, Kintamani, Besakih, and Payangan Village.  Pollen from intact anthers were stored at three different storage temperatures, i.e., 25oC (room temperature), 4oC (refrigerator), and -20oC (freezer), with or without oven dried at 40oC for 20 hours before storage.  Pollen viability was determined using in vitro germination test.  Germination media contained 20 g/l sucrose and 8 g/l agar.  Result shows that -20oC was the best storage temperature.  At this temperature, only slight reduction of pollen viability after 2 months storage.  Storage at room temperature (25oC – 30oC) was not effective; pollen was infected with fungus within a week of storage. Oven dried pre-treatment before storage made better performance on pollen stored at 4oC.  Pollen moisture content decreased after oven dried, which also decreased fungus infection.  Storage of pollen is an important aspect to plant breeding programs, when individuals to be hybridized do not flower at the same time or are geographically separated.  This is the first report on pollen storage and viability of Citrus genotypes grown in Bali. 
POTENSI EKTRAK CACING LAUT Perinereis aibuhitensis SEBAGAI ANTIMIKROBA TERHADAP BAKTERI Staphylococcus aureus dan JAMUR Malassezia furfur Andi Evi Erviani
Scripta Biologica Vol 6, No 3 (2019)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

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Abstract

Penyakit akibat infeksi mikroba semakin sering terjadi. Penelitian tentang potensi ekstrak cacing laut Perinereis aibuhitensis sebagai antimikroba terhadap bakteri  Staphylococcus aureus dan jamur Malassezia furfur telah dilakukan. Tujuan penelitian ini untuk mengetahui konsentrasi ekstrak cacing laut Perinereis aibuhitensis yang efektif dalam menghambat pertumbuhan bakteri Staphylococcus aureus dan pertumbuhan jamur Malassezia furfur. Hasil penelitian yang diperoleh menunjukkan bahwa ekstrak cacing laut yang menggunakan pelarut etanol 96 % dan diperlakukan pada kultur bakteri Staphylococcus aureus dengan konsentrasi ekstrak cacing laut 7.5%, 15%, 30% dan 60% memperlihatkan terbentuknya zona hambat pada media dengan lama inkubasi 1x24 jam dan 2x24 jam, dan memperlihatkan zona hambat terbesar pada konsentrasi ekstrak 60%. Demikian pula pada Jamur Malassezia furfur, terbentuk zona hambat pada konsentrasi ekstrak 7,5%;15%;30% dan 60%. Zona hambat terbesar terlihat pada konsentrasi ekstrak 60%. Dari hasil penelitian yang telah dilakukan dapat disimpulkan bahwa ekstrak cacing laut Perinereis aibuhitensis berpotensi sebagai antimikroba terhadap bakteri Staphylococcus aureus dan jamur Malassezia furfur  .Key Words: Perinereis aibuhitensis, antimikroba, Staphylococcus aureus, Malassezia furfur
APPEARANCE OF THE MOURNING GECKO, Lepidodactylus lugubris (DUMÉRIL & BIBRON, 1836), IN THE CAGE OF ORIENTAL WHITE-EYE (Zosterops palpebrosus) Rury Eprilurahman
Scripta Biologica Vol 6, No 2 (2019)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

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Abstract

The nocturnal parthenogenetic species, mourning gecko (Lepidodactylus lugubris), has been recorded in Sleman, Daerah Istimewa Yogyakarta, Java, Indonesia. There has yet no specific information of its distribution in Java and its behaviour. One out of three individues observed was successfully caught. A single specimen collected from The Oriental white-eye (Zosterops palpebrosus) cage is reviewed along with literature reports of the occurrence of the species. It is likely that population of this invasive gecko has been established in the region.
ANALISIS IN SILICO PERANCANGAN crRNA CRISPR DENGAN TARGET SEKUENS PENYANDI SQUALENE SYNTHASE PADA Artemisia annua Linn. Ivan Tjahja Pranata
Scripta Biologica Vol 6, No 4 (2019)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.sb.2019.6.4.1105

Abstract

Artemisinin adalah salah satu jenis fitofarmaka yang penting oleh karena beberapa potensi medisnya. Meskipun demikian, produktivitas Artemisia annua di Indonesia secara umum masih berkisar di bawah 0,5% sehingga secara komersial belum layak untuk dibudidayakan. Salah satu faktor utama yang menghambat produktivitas artemisinin adalah keberadaan gen SQS yang berkompetisi dengan gen ADS dalam penggunaan susbtrat farnesil difosfat. Kompetisi ini menurunkan kadar prekursor kunci bagi pembentukan senyawa artemisinin. Teknik penyuntingan genom CRISPR berbasis non-GMO dapat diaplikasikan untuk mengatasi hambatan ini. Mekanisme knockout yang disebabkan oleh sistem CRISPR pada gen SQS dapat menekan tingkat kompetisi substrat yang terjadi pada jalur biosintesis artemisinin sehingga diharapkan meningkatkan kadar artemisinin. Sebelum dilakukan transformasi secara in vitro, perlu dilakukan analisis in silico untuk pembuatan model crRNA sintetis. Dalam penelitian ini, digunakan 2 jenis perangkat lunak berbasis bioinformatika yaitu E-CRISP dan CRISPRdirect untuk pelacakan on-target dan off-target. Berdasarkan hasil perancangan yang telah dilakukan, 10 desain crRNA sintetis berhasil dibentuk dengan model crRNA_F5 sebagai desain yang paling optimal. Model crRNA_F5 ini memiliki sekuens protospacer 5’- GGTGTAGTCAAATTGAGACG-3’ dan sekuens PAM 5’-TGG-3’. Pemilihan desain crRNA_F5 dipertimbangkan dari nilai efficacy sebesar 78.57, skor Doench dan skor Xu tertinggi, serta minimalisasi jumlah off-target pada sekuens 8mer pada jumlah 259. Model ini sama sekali tidak memiliki off-target pada sekuens 20mer dan 12mer.
GROWTH OF SEAWEED Gracilaria verrucosa CULTURED ON DIFFERENT INITIAL WEIGHT WITH LONGLINE METHODS IN KARIMUNJAWA WATERS TITIK SUSILOWATI; DICKY HARWANTO; CHONDRORESMI BANOR FAWWAZ; ALFABETIAN HARJUNO CONDRO HADITOMO; SARJITO SARJITO
Scripta Biologica Vol 6, No 4 (2019)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1019.551 KB) | DOI: 10.20884/1.sb.2018.5.4.1120

Abstract

One factor that can optimize seaweed production is determining the initial weight of planting. However, planting seaweed in open waters is very vulnerable to aggregation from herbivorous fish. This study aims to determine the best initial weight for the growth of G. verrucosa, which is cultivated in net cage by the longline method. Net cages, made of nylon, are applied as protection for G. verrucosa from aggregation of herbivorous fish. This research was conducted in Karimunjawa waters, Jepara Regency, Central Java, for 42 days. The experimental design used was Randomized Block Design (RBD), with three treatments and four replications each. The treatment was different initial planting weights of 25, 50, and 75 g. The observed variables included culture techniques, relative growth rates, specific growth rates, and water quality. The analysis showed that the different initial weight was very significant (P <0.01) on the growth of G. verrucosa. Treatment with an initial weight of 25 g gave the best relative growth rate (2.07±0.25%.d-1), and the best specific growth rate (1.48±0.13%.d-1). Furthermore, this study was able to prove that the use of a net planting cage on a seaweed hanger can avoid aggregation of herbivorous fish, which is indicated by the growth of G. verrucosa.
EFEK TERAPI PISANG TONGKA LANGIT (Musa troglodytarum) TERHADAP INDEKS ERITROSIT MENCIT (Mus musculus) MODEL MALARIA Efraim Samson
Scripta Biologica Vol 6, No 4 (2019)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.sb.2019.6.4.1114

Abstract

Malaria is an infectious disease that is still a major health problem in various countries, including Indonesia. Malaria parasite infection can induce changes in structure, physiology and biochemistry in hematology causing damage to erythrocytes. The use of plants in malaria treatment (phytotherapy) began to be developed at this time. Tongka langit banana (Musa troglodytarum) is one type of banana that has the potential to be developed. This study aimed to see the therapeutic effect of tongka langit banana against the erythrocyte index of mice with malaria models. The results showed that the therapy of tongka langit banana was able to increase the erythrocyte index (RBC, MCV, MCH, and MCHC) in mice with malaria, and the most effective dose is 0.75 gr/mice/day.Key Words: Tongka Langit Banana, Erythrocyte Index, Phytotherapy, Malaria.
SKIRINING FITOKIMIA DAN UJI ANTIAGREGASI PLATELET EKSTRAK DAUN LAMPENI (Ardisia humilis) ASAL PULAU PEUCANG TAMAN NASIONAL UJUNG KULON Fransiska Tinambunan
Scripta Biologica Vol 9, No 1 (2022)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.sb.2022.9.1.1341

Abstract

Lampeni (Ardisia humilis) is a plant that can be found in Peucang Island, Ujung Kulon National Park. Lampeni are used as medicinal plants because they can produce secondary metabolites. The purpose of this study was to determine the phytochemical content and antiaggregation platelets potential of lampeni leaf. This research was conducted in September-October 2018 on Peucang Island and UNJ Biochemistry Laboratory. The phytochemical content of the lampeni leaf was in the form of alkaloids, flavonoids, terpenoids, saponins and tannins and platelet aggregation test using the Pimentel method. Lampeni leaf extract was made using simplicia and solvent which is 1:10 (w/v) with 70% ethanol solvent.  The results of the phytochemicals content test on the lampeni leaf contain alkaloids, flavonoids, terpenoids and saponins but do not contain tannins. The results of platelet aggregation test showed there was no formation of violet gel which indicated that platelet aggregation did not occur so that the lampeni leaf had the ability as an anti-aggregate platelet.

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