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Scripta Biologica
Published by Universitas Jenderal Soedirman
ISSN : -     EISSN : 23553138     DOI : -
Core Subject : Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology
Scripta Biologica is a peer reviewed open access electronic journal published by the Faculty of Biology Jenderal Soedirman University, publishes research papers encompasses all aspects of biology, including; molecular biology, cell biology, and genetics; physiology and reproduction; ecology and conservation; biodiversity, evolution, taxonomy, and biogeography.
Arjuna Subject : -
Articles 176 Documents
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BAY-LEAVES (SYZYGIUM POLYANTHUM) EXTRACTS PREVENTED THE DECREASE OF SPATIAL MEMORY IN MALE BALB/C MICE (MUS MUSCULUS) WITH BRAIN AGING Dewi Puspasari
Scripta Biologica Vol 9, No 1 (2022)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.sb.2022.9.1.1319

Abstract

AbstraCTIntroduction: Memory impairment is one of the symptoms of aging process which can be caused by either free radicals or oxidative stress. Antioxidants such as flavonoids are compounds that can inhibit reactive oxygen species (ROS). Bay-Leaves (Syzygium polyanthum) is one of the natural compound that contains high flavonoids. The purpose of this study was to prove that Bay-Leaves (Syzygium polyanthum) extracts prevent spatial memory impairment in male Balb/c mice (Mus musculus) with brain aging.Methods: This study was a true experimental study with randomized pretest-posttest control group design. The experimental animals were 14 mice, which were divided into two groups (7 each group). Mice that were included in this study were healthy (active and willing to eat), weighing 20-22 grams, 3-month-old With oral D-galactose (300mg/kgBW/day)induced brain aging for 28 days. The control group was treated a placebo (2 ml of aquabidest), and the treatment group was treated with oral Bay-leaves extracts at a dose of 5.25 mg/kgBW/day. Spatial memory was examined using the Morris Water Maze method using Probe Test to assess spatial memory retention and the Number of Platform Crossing Test. The examinations were done before (pretest) and after treatment (posttest) with placebo and Bay-leaves extract for 28 days.Results: The results showed that in the Probe Test, before the treatment (pretest) in the control and treatment groups there were no differences (69.94 ± 4.46% vs 69.42 ± 4.30%; p = 0.831), but after treatment during 28 days (posttest), the mean of the Probe Test between groups was significantly different (52.02 ± 7.46% vs 66.08 ± 3.72%; p = 0.001). In the Number of Platform Crossings, before the treatment (pretest) there was no difference in the Number of Platform Crossings (6.92 ± 0.640 times vs. 7.10 ± 0.748 times; p = 0.640), but after treatment for 28 days (posttest), the mean Number of Platform Crossings between groups was significantly different (4.67 ± 0.590 times vs 6.46 ± 0.809 times; p <0.001). For intergroup tests, the Probe Test in the control group decreased significantly (from 69.94 ± 4.46% to 52.02 ± 7.46%; p = 0.001), whereas in the treatment group there was no decreased (69.42 ± 4.30% to 66.08 ± 3.72%; p = 0.060). In the Number of Platform Crossings test, there was a significant decrease in the control group (6.92 ± 0.640 to 4.67 ± 0.590, p = <0.001), whereas in the treatment group there was no decreased (7.10 ± 0.748 to 6.46 ± 0.809, p = 0.070).Conclusion: Based on the results of this study, it can be concluded that Bay-Leaves (Syzygium polyanthum) extracts prevented the decrease of spatial memory in male Balb/c mice (Mus musculus) with brain aging.
PERFORMA REPRODUKSI IKAN NILEM (Osteochilus hasselti C.V.) BETINA TERPAPAR MEDIA MENGANDUNG K2Cr2O7 SEBAGAI ENDOCRINE DISRUPTOR AGENT Purwo Raharjo; Windiriani Lestari; Gratiana Ekaningsih Wijayanti
Scripta Biologica Vol 9, No 1 (2022)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.sb.2022.9.1.886

Abstract

Heavy metals can generally act as EDCs. Likewise chromium hexavalent may serve as a Endocrine Disrupter, especially the fish reproductive system. This study aims to determine the effect of chromium hexavalent on estradiol content, ovarian development and liver of nilem female (Osteochilus hasselti C.V.), the researchers also want to see the dose of chromium hexavalent most influential on estradiol levels, ovarian development, and liver of nilem fish (Osteochilus hasselti C.V.) as well as the interaction between reproduction parameters with water quality of maintenance media of nilem fish (Osteochilus hasselti C.V.).This study uses the method of "quantitative experimental". The experimental design was Completely Randomized Design with treatment of chromium hexavalent dose of 0, 0.009, 0.018, 0.027 and 0.036 mg/L performed in 5 replications at 3 stages, ie 1st, 2nd, and 3th months post-spawning maintenance. At 1st month of maintenance, chromium hexavalent did not significantly affect the reproductive parameters of GSI (Gonadosomatic Index), fecundity, HSI and estradiol. The chromium hexavalent had no significant effect on GSI, fecundity and estradiol and significantly affected HSI at 2nd months of maintenance. Chromium hexavalent significantly affected GSI, fecundity, HSI and estradiol in a 3th month maintenance trial.
MORPHOMETRIC OF THE REPRODUCTIVE ORGAN OF FEMALE BANDICOOT-THE COMMON ECHYMIPERA (Echymipera calubu) Angel N Tethool; Fahry Rafli; Freddy Pattiselanno
Scripta Biologica Vol 9, No 1 (2022)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.sb.2022.9.1.1209

Abstract

A study was conducted at the Laboratory Animal Physiology and Reproduction Laboratory, Faculty of Animal Science Universitas Papua between 15 June and 15 September 2017. Three female reproductive organs of bandicoot (Echymipera kalubu) were used, and the morphology of the reproductive organ was examined. This paper presents relevant data for further marsupial reproduction studies.
POTENSI DAN KEKAYAAN Ficus spp. PADA ZONA REHABILITASI BLOK PASIRBATANG DI TAMAN NASIONAL GUNUNG CIREMAI KABUPATEN KUNINGAN Yayan Hendrayana
Scripta Biologica Vol 9, No 1 (2022)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.sb.2022.9.1.986

Abstract

The Pasirbatang rehabilitation zone is part of the Ciremai Mountain National Park (TNGC) which is on the south side has experienced degradation. Therefore, planting was carried out in this zone with local species in 2014. The purpose of this study was to determine the vegetation structure and richness of the Ficus clan in the rehabilitation zone. This research was conducted in July-September 2018 with the method of placing the sampling plot on the specified path. A total of 31 species were found at various levels of growth with the Important Value Index (IVI) at the tree level (192.12%) in the form of Pinus merkusii, pole level (66.38%) in the form of Nauclea lanceolata, sapling level (141.72%) and seedlings (148.69%) in the form of Caliandra callothyrsus. Potential of Ficus spp is spread in various growth rates, namely species of F. septica, F. ampelas, F. variegata and F. fistulosa with the highest density at seedling level (166 ind / ha), and followed by sapling levels (64 ind / ha), poles (16 ind / ha) and trees (0.67 ind / ha). This Ficus wealth is considered important for the zone rehabilitation program in the Mount Ciremai National Park
The Application of Two Steps Culture in Agarwood, Aquilaria malaccensis, In Vitro Culture Improves Microshoots Induction and Development Vindi Ambara Putri; Sugiyono Sugiyono; Lucky Prayoga; Rendie Prasetyo; Sharon Hilary
Scripta Biologica Vol 9, No 1 (2022)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.sb.2022.9.1.1373

Abstract

Agarwood (Aquilaria malaccensis) is an important species with high economic value and has many benefits, which led to an overexploitation in its natural habitat.  An effort to both provides sufficient seedling for agarwood cultivation and conservation is therefore much needed. This study has been carried out with a view to determine the effect of media types and BAP concentrations in two steps cultivation system on agarwood microshoot induction. This was a two stage-experiments i.e., microshoots induction and optimizing shoot development.  The research results showed that the interaction between different media type and BAP concentrations had no significant effect on agarwood microshoot induction.  Subsequent culture on MS media without any BAP addition showed that explant derived from MS medium solidified with 2.5 gr. L-1 Phytagel produced 2.36 ± 0.48 shoots/explant and 3.69 ± 1.16 leaves/explant.  In addition, explant derived from culture on MS medium supplemented with 4 µM BAP produced 2.28 ± 0.61 shoots/explant. This is for the first time the application of two steps culture system for Agarwood (Aquilaria malaccensis) has been deployed and how the habituation phenomenon is handled.
PROXIMATE COMPOSITION OF Reinwardtiodendron humile (Hassk.) Mabb. FRUIT FROM BOGOR BOTANIC GARDENS COLLECTION Saniyatun Mar&#039;atus Solihah; Inggit Puji Astuti
Scripta Biologica Vol 9, No 1 (2022)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.sb.2022.9.1.1148

Abstract

Reinwardtiodendron humile (Hassk.) Mabb. is one of the members of the Meliaceae tribe with the potential to be developed and consumed as fresh fruit or processed fruit. This research aims to determine the proximate composition or nutritional values contained in R. humile. Proximate analysis of R. humile included analytical determinations of water, ash, crude fat, protein, crude fibre, and carbohydrates. All tests for each of the parameters or components were carried out two times. Then, the contents determined through proximate tests were calculated using certain formulas. The results of this study indicate that the proximate composition of R. humile consisted of water, ash, fat, protein, crude fibre, and carbohydrates (74.2% - 75.28%; 1.35% - 1.54%; 0.53% - 0.72%; 2.48% - 2.73%; 0.86% - 3, 47%; and 16.7% - 20.14%). These results meant the fruit contents met the quality standards of fruit, except for the protein content. The results of the analysis of the proximate composition also show that the nutritional values of R. humile fruit did not significantly differ from the nutritional values of other fruits of the Meliaceae tribe commonly consumed by humans, such as duku, langsat, and kokosan.
Kepadatan, Pola Distribusi dan Pola Pertumbuhan Udang Putih (Penaeus merguiensis de Man) di Perairan Estuari Margasatwa Karang Gading Wardinsen Situmorang
Scripta Biologica Vol 9, No 1 (2022)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.sb.2022.9.1.1389

Abstract

AbstractResearch on the density, distributionpattern and growth pattern of White Shrimp (Penaeus merguiensis de Man) in the Estuary Waters of the Karang Gading Wildlife Reserve, Deli Serdang Regency was carried out from June to August 2021. White shrimp samples were carried out for three days at three stations with the same time for 2 days. month of observation. The sampling point was determined by using the purposive sampling method. Samples were taken with a mini purse seine and the water physicochemical factors were identified at the Agricultural Laboratory of the University of North Sumatra. The results showed that the density of white shrimp was 4.50 ind/m2-7.33 ind/m2 with the highest density value found at station  I of 7.33 ind/m2 and the lowest was 4.50 ind/m2, the distribution pattern of white shrimp with the range of  I d 3.06-3.18 refers to grouping criteria, the growth pattern of white shrimp is allometric (-) with b 2.201 (I), 2.686 (II), 2.722 (III), the value of substrate fraction at station I (sand 24, 5%, 40% silt, 35.5% clay, dusty clay substrate type), station II (41.5% sand, 44% silt, 14.5 clay, clay substrate type), station III (43% sand, silt 28%, 29% clay, sandy loam substrate type). While the relationship of density with water chemistry factors obtained temperature, brightness, depth, current velocity, dissolved oxygen, BOD5, DO and phosphate to the density index of white shrimp obtained in the opposite direction of correlation (-), and the direction of correlation in the direction of (+) pH, salinity, and nitrate.Key words: Density, distribution pattern, growth pattern, substrate  fraction, water physicochemical factors AbstrakPenelitian mengenai kepadatan, pola distribusi dan pola pertumbuhan Udang Putih (Penaeus merguiensis de Man) di Perairan Estuari Suaka Margasatwa Karang Gading Kabupaten Deli Serdang telah dilakukan pada bulan Juni hingga Agustus 2021. Sampel udang putih dillakukan tiga hari pada tiga stasiun dengan waktu yang sama selama 2 bulan pengamatan . Titik pengambilan sampel ditentukan dengan menggunakan metode Purposive sampling. Sampel diambil dengan pukat cincin mini dan faktor fisika kimia air diidentifikasi dilakukan di Laboratorium Pertanian Universitas Sumatera Utara.  Hasil penelitian didapatkan kepadatan udang putih sebesar 4,50 ind/m2-7,33 ind/m2  dengan nilai kepadatan tertinggi terdapat pada stasiun I sebesar 7,33 ind/m2  dan terendah sebesar 4,50 ind/m2, pola distribusi udang putih dengan kisaran Id 3,06-3,18 mengacu  pada kriteria berkelompok, pola pertumbuhan udang putih bersifat allometrik (-) dengan b 2,201 (I), 2,686 (II), 2,722 (III), nilai fraksi substrat pada staisun I (pasir 24,5%, debu 40%, liat 35,5%, tipe substrat lempung berdebu), stasiun II (pasir 41,5%, debu 44%, liat 14,5, tipe substrat lempung), stasiun III (pasir 43%, debu 28%, liat 29%, tipe substrat lempung berpasir). Sedangkan hubungan kepadatan dengan faktor-kimia air didapatkan suhu, kecerahan, kedalaman, kecepatan arus, oksigen terlarut, BOD5, DO dan fosfat terhadap indeks kepadatan udang putih didapatkan arah korelasi berlawanan (-), dan arah korelasi searah (+) pH, salinitas, dan nitrat.Kata kunci : Kepadatan, pola distribusi, pola pertumbuhan, fraksi substrat, faktor fisik kimia air
STUDI EKOLOGI Ficus spp. DI HUTAN TANGKAHAN KABUPATEN LANGKAT SUMATERA UTARA FARAHNAZ AFRILIANDINI LUBIS
Scripta Biologica Vol 9, No 2 (2022)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.sb.2022.9.2.1390

Abstract

Ficus spp. merupakan salah satu tumbuhan dari suku Moraceae yang sering dikenal dengan beringin-beringinan atau pohon ara. Jenis tumbuhan Ficus melimpah di hutan dataran rendah dan memegang peranan penting dalam ekologi. Kawasan hutan Tangkahan merupakan salah satu kawasan hutan konservasi yang terletak di Sumatera Utara dan lokasi ditemukan jenis Ficus spp. Hal ini bertujuan untuk menganalisis faktor ekologi tumbuhan Ficus spp. dan memperoleh informasi mengenai pemanfaatannya di kawasan hutan Tangkahan, yang dilaksanakan pada bulan Maret hingga Juni 2020. Pengambilan sampel dilakukan dengan menggunakan metode purposive sampling yaitu memilih lokasi secara sengaja. Data vegetasi di analisis dengan nilai kerapatan (K), Indeks Keanekaragaman (H’), Indeks Keseragaman (E) dan Indeks Similaritas (IS). Karbon tersimpan dianalisis dengan persamaan allometrik Kettring’s dan unsur hara tanah dianalisis di laboratorium. Diperoleh 12 jenis Ficus dengan 576 individu/ha. Kerapatan jenis Ficus spp. pada seluruh tingkat pertumbuhan berkisar antara 1–11 individu/ha. Indeks keanekaragaman seluruh tingkat pertumbuhan tergolong sedang dan Indeks keseragaman seluruh tingkat pertumbuhan tergolong tinggi. Indeks Similaritas berkisar antara 16.67–75%. Karbon tersimpan pada lokasi ini sebesar 8.74 ton C/ha. Analasis unsur tanah dilakukan pada katagori C-organik, N-total, P-Bray I, K-dd, Ca, Mg dan pH.
PENGARUH DIET PROTEIN DAN SUPLEMENTASI VITAMIN D3 PADA MASA MENYUSUI TERHADAP ERUPSI GIGI INSISIVUS SENTRALIS MANDIBULA ANAK TIKUS MALNUTRISI Riski Amalia Hidayah; Rakmawati Rakmawati; Aris Aji Kurniawan; Anindita Laksitasari; Meylida Ichsyani
Scripta Biologica Vol 10, No 1 (2023)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.sb.2023.10.1.1296

Abstract

ABSTRAKMalnutrisi akibat kekurangan protein saat kehamilan berisiko melahirkan bayi berat badan lahir rendah (BBLR) dan berdampak pada pertumbuhan tulang dan gigi anak. Kekurangan protein saat kehamilan dapat menyebabkan terjadinya gangguan mineralisasi gigi, ganggguan maturasi gigi, hambatan erupsi gigi,  kegoyahan gigi bahkan kehilangan gigi akibat penurunan kualitas tulang alveolar. Protein sangat dibutuhkan dalam proses metabolisme kalsium dalam tulang dan gigi. Vitamin D3 berperan dalam homeostasis kalsium dan fosfor yang dibutuhkan untuk mineralisasi jaringan keras termasuk tulang dan gigi. Tujuan dari penelitian ini adalah untuk mengetahui umur pertama erupsi dan panjang pertumbuhan gigi insisivus mandibula sentralis kanan dan kiri saat masa erupsi pada anak malnutrisi yang menyusu pada induk dengan perlakuan diet protein dan suplementasi vitamin D3. Penelitian ini termasuk jenis penelitian eksperimental laboratoris dengan rancangan post test only control group design.  Sampel penelitian menggunakan 30 ekor tikus Rattus novergicus galur wistar yang dibagi menjadi 5 kelompok yaitu 2 kelompok kontrol meliputi kontrol positif (K+) berupa anak tikus dengan berat badan (BB) normal yang menyusu pada induk yang diberikan perlakuan diet protein standar dan suplementasi vitamin D dosis 0,036 IU/ hari peroral serta kontrol negatif (K-) berupa anak tikus malnutrisi yang menyusu pada induk yang diberikan perlakuan diet protein rendah selama masa menyusui. Kelompok perlakuan meliputi 3 kelompok anak tikus malnutrisi yang menyusu pada induk dengan diet protein standar dengan suplementasi vitamin D dosis 0,036 IU/ hari peroral (P1), diet protein standar dengan suplementasi vitamin D dosis 0,036 IU/ hari peroral (P2) serta diet protein standar saja selama masa menyusui. Hari pertama erupsi diukur secara visual dan panjang pertumbuhan gigi diukur pada umur 14 hari dan 22 hari dengan menggunakan sliding caliper. Hasil penelitian menunjukkan bahwa erupsi paling cepat terjadi pada kelompok K+ kemudian P1. Kelompok P2 dan P3 mengalami kecepatan erupsi yang sama yaitu pada rerata umur 10,2 hari, sedangkan kelompok K- mengalami erupsi gigi paling lambat yaitu mencapai rerata umur 11,5 hari. Pemberian diet protein standar dan vitamin D3 dosis 0,036 IU/hari peroral menunjukkan perbedaan yang bermakna terhadap panjang pertumbuhan gigi. Pemberian diet protein standar menunjukkan perbedaan bermakna terhadap panjang pertumbuhan gigi. Pemberian diet protein rendah dan vitamin D3 dosis 0,036 IU/hari peroral menunjukkan perbedaan bermakna terhadap panjang pertumbuhan gigi insisivus sentralis mandibula kiri namun tidak terdapat perbedaan yang bermakna pada regio kanan. Kombinasi diet protein standar dengan vitamin D3 dosis 0,036 IU/ hari per oral yang saat masa menyusui mempercepat waktu erupi gigi dan meningkatkan panjang pertumbuhan gigi insisivus sentralis mandibula kanan dan kiri anak tikus dari umur 14 hingga 22 hari. 
ETNOBOTANI TUMBUHAN DI DESA SERUMPUN KABUPATEN SAMBAS YANG DIMANFAATKAN SEBAGAI KOSMETIK Sisi Arlina; Syamswisna Syamswisna; Hayatul Fajri
Scripta Biologica Vol 10, No 2 (2023)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.sb.2023.10.2.1378

Abstract

People of Serumpun village Sambas district usually use plants as traditional cosmetics. Especially, for betangas (traditional sauna) and bekasai (traditional scrub). The plant used as a cosmetic has been used for generations and maintained by people of Serumpun village to this day. This research aimed to observed the plant traditionally used as a cosmetic in Serumpun village Sambas district. Forty-seven species from 30 families were used as the cosmetic source. The most family used for cosmetic are Rutaceae and Zingiberaceae. Fruit is the most plant part used for cosmetics, and people of Serumpun village primarily use the plant for skincare.

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