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Scripta Biologica

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Published by Universitas Jenderal Soedirman

ISSN : - EISSN : 23553138 DOI : -

Core Subject : Science, Agriculture,

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

Scripta Biologica is a peer reviewed open access electronic journal published by the Faculty of Biology Jenderal Soedirman University, publishes research papers encompasses all aspects of biology, including; molecular biology, cell biology, and genetics; physiology and reproduction; ecology and conservation; biodiversity, evolution, taxonomy, and biogeography.

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Articles 176 Documents

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PROFIL DARAH KUSKUS PHALANGERIDAE DI DATARAN RENDAH PAPUA Febriza Dwiranti; Ursula Paulawati Maker; Aksamina Maria Yohanita
Scripta Biologica Vol 5, No 2 (2018)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar

The cuscus study for approximately 15 years showed that there are morphological and morphometric differences among the same species. Cuscus is a nocturnal animal that very dependent on natural forests, which in fact is beginning to decrease. Environmental and food changes are the cause of changes in the blood profile. Therefore, this research needs to be done with the aim of obtaining blood profiles, preparing blood smear from each type of cuscus from the lowlands and islands in Papua. The results of this study describe blood profiles of three species of cuscus found Spilocuscus maculatus, Spilocuscus papuensis and Phalanger orientalis. Types of Leukocytes found consisting of Basophils, Eosinophils, stem Neutrophils, segment Neutrophils, Lymphocytes and Monocytes in S. maculatus; Basophils, Eosinophils, stem Neutrophils, segment Neutrophil, Lymphocytes in S. papuensis; and Basophils, Eosinophils, segment Neutrophils, Lymphocytes, Monocytes on P. orientalis. The largest number of leukocytes is S. maculatus ♂ adult (12,20x103 / mm3). The highest amount of Eristrosit is P. orientalis ♂ adult (10.780.000 / mm3). The highest hemoglobin belongs to S. maculatus ♂ adult is 14.8 mg / dL, and the highest percentage of hematocrit is also S. maculatus ♂ adults (44%). The three species of cuscus have different numbers of leukocytes, erythrocytes, haemoglobin and hematocrit.

KELIMPAHAN CHLOROPHYTA PADA MEDIA BUDIDAYA IKAN NILA YANG DIBERI PAKAN FERMENTASI DENGAN PENAMBAHAN TEPUNG KULIT UBI KAYU DAN PROBIOTIK Heni Andriyani; Endang Widyastuti; Dwi Sunu Widyartini
Scripta Biologica Vol 1, No 1 (2014)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Chlorophyta is autotroph organisms which has an important role in fresh waters as one of the largest algae division. This study aimed to determine the abundance and similarity structure of Chlorophyta in Tilapia culture media which was given fermented feed with the addition of cassava peel flour and MEP+ probiotic. This study used four treatments with four replications: (A) fermented feed without addition of cassava peel flour or 0% and MEP+ probiotic administration in media, (B) 25%, (C) 50% and (D) 75%. Sampling was carried out 6 times at intervals of 2 weeks. The main parameters were the number of Chlorophyta species and individuals,while supporting parameters were physical (temperature and TDS) and chemical (DO, BOD, pH, NO3, NO2 and total of PO4). The abundance of Chlorophyta data were analyzed descriptively and its similarity structure were analyzed using Cluster analysis. Then, continued with SIMPER analysis to determine the contribution of species to abundance similarity with PRIMER-E v.5 software. Analysis results showed that the abundance of Chlorophyta consists of 33 species with the average number of 10.412 individuals/liter. Cluster analysis results based on Bray-Curtis similarity index had a quite high similarity and it ranged between 57.79% -68.84%. SIMPER analysis results showed that the species which given highest contribution were Kircheneriella lunaris (31,03%), Selenastrum sp. (21,69%), and Gonatozygon monotaenium (12,96%).

INTERTIDAL BARNACLE COMMUNITY OF KETAPANG AND GILIMANUK PORTS THAT SEPARATED BY THE INDONESIAN THROUGHFLOW OF BALI STRAIT Sulistiono Sulistiono; Diah Etika Maharatih Setiarina; Romanus Edy Prabowo
Scripta Biologica Vol 4, No 4 (2017)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Indonesian Throughflow (ITF) flows from the Pacific Ocean through the western route of Makassar Strait and exit directly to the Indian Ocean through Lombok Strait and Bali Strait or flows eastward through the Banda Sea. Bali Strait separates the Ketapang Ferry Port of Banyuwangi on the Jawa side and Gilimanuk Ferry Port of Bali. Ferries connect Jawa and Bali through those ports as the primary mean of transportation. Ship hull and ballast water of those ships act as a proxy for barnacle distribution in their larval stage from one harbor to another so that it could influence the barnacle community of those two ports. The environmental condition of each seaport defines the barnacle community based on the adaptation ability of barnacle. The purpose of this research was to determine the intertidal barnacle diversity on both Ketapang and Gilimanuk seaports and to compare the barnacle community between Ketapang and Gilimanuk seaports. This study was a survey, and simple random sampling technique was used to collect samples. Sample collection was conducted from July to August 2017, and the sample observation and identification were performed at the Faculty of Agriculture Laboratory at Banyuwangi PGRI University. The diversity indexes including Shannon, Brillouin, Simpson, and Evenness were calculated to determine the level of diversity, and Bray-Curtis similarity coefficient was used to compare the intertidal barnacle community. The result showed the intertidal barnacle diversity of Ketapang and Gilimanuk seaports were low, at the level of 0.89, 0.89, 0.41 and 0.27 at Ketapang Ferry Port and 0.81, 0.80, 0.43, and 0.28 at Gilimanuk Ferry Port based on Shannon, Brillouin, Simpson, and Evenness accordingly. The two ports were having a medium similarity of intertidal barnacle community indicated by the Bray-Curtis similarity coefficient analysis score of 0.58.

EFEKTIFITAS BAKTERI ENDOFIT DAN PENAMBAHAN INDOLE ACETIC ACID (IAA) DALAM MENINGKATKAN PERTUMBUHAN TANAMAN PADI Oryza sativa L. Aria Rizki Ramadhan; Oedjijono Oedjijono; Ratih Dewi Hastuti
Scripta Biologica Vol 4, No 3 (2017)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Rice plant needs essential and non-essential nutrients to grow. However, the use of inorganic fertilizers may affect the soil microbe community which expected to increase the availability of nutrients for both plants and microbes, a condition that could control several types of diseases. Many efforts were made to find alternative fertilizer which environmentally friendly, for instance by exploiting microorganisms such as endophytic bacteria associated with plant tissue or seed plant cell. This research aimed to determine the effect of endophytic bacteria, the concentration of IAA, and the combination of endophytic bacteria with a concentration of IAA, on the growth of rice plant. This research was conducted at the Laboratory of Soil Biology, The Soil Research Institute located in Cimanggu Bogor from December 2015 to February 2016. This research was a Completely Randomized Design (CRD) with two factors, i.e., the inoculant of endophytic bacteria (2.2 KT, KR 6, and I CM), and the concentration of IAA (0, 0.01, 0.1, 0.5, and 1 ppm). Three replicates were applied for each treatment of the inoculant combined with different doses of IAA. The variety of rice plants tested were Inpari 13 and IR 64. The potential test has been applied to determine the factor with the highest yield of the rice plant growth. The parameters observed were the height of the rice plant, the length of the rice plant roots, and the total weight of the plant including root. The results showed the inocula of endophytic bacteria alone had no effect to increase growth for both IR 64 and Inpari 13 rice variety. The IAA concentration of 0.1 ppm was able to increase the height of rice plant and the length of rice plant root for both IR 64 rice plant and Inpari 13. The combined treatment of KR 6 bacterial inoculum with 1 ppm of IAA concentration was effectively promoted rice plant growth for both IR 64 and Inpari 13.

PENGARUH LAMA PENYIMPANAN DAN KONSENTRASI Bifidobacterium BBIV PADA YOGHURT TERHADAP KADAR ASAM LAKTAT Hafidh Syaifuddin; Dyah Fitri Kusharyati; Pancrasia Maria Hendrati
Scripta Biologica Vol 1, No 3 (2014)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.sb.2014.1.3.567

Yogurt is a well-known fermented dairy product. The addition of Bifidobacterium BBIV into yogurt was able to increase the content of lactic acid and therefore was able to inhibit the growth of Salmonella typhi. This study aimed to find out the amount of lactic acid in yogurt added by the culture of Bifidobacterium BBIV in the various concentration and storage periods; to evaluate antibacterial activity against Salmonella typhi. This research was experimental using a Completely Randomized Design. The proportion of the combination of Bifidobacterium BBIV starter (Lactobacillus bulgaricus: Streptococcus thermophillus: Bifidobacterium BBIV) were 1:1:0 (B0), 1:1:0,5 (B1), 1:1:1 (B2) and 1:1:1,5 (B3). The storage period was 30-days, observations were made every five days. This study computed the ANOVA followed by the LSD test for data analysis. The result showed that the addition of Bifidobacterium BBIV starter in various proportion to the yogurt with the different storage periods gave significantly different effects on the lactic acid amount. The addition of Bifidobacterium BBIV starter of 1:1:1(B2) proportion stored after 20 days showed an increasing level of lactic acid. All of the yogurt with any other ratios (B0, B1, B2, and B3) of Bifidobacterium BBIV starter, stored after 30 days were still able to inhibit the growth of Salmonella typhi. The organoleptic test showed that yogurt with 1:1:1,5 (B3) starter ratio was the most preferred in both taste, aroma, color, and homogeneity. The study suggested that the amount of lactic acid in the yogurt increased by the addition of Bifidobacterium BBIV in various ratios, i.e., 1:1:0,5; 1:1:1; and 1:1:1,5 after 30 days were 1,2652%;1,2243% and 1,2506% respectively. The inhibition zone produced by yogurt on the bacterial growth of Salmonella typhi was 6,5–8,3 millimeters in any of the storage periods.

STRUKTUR DAN VEGETASI TUMBUHAN BAWAH PADA TEGAKAN PINUS DI RPH KALIRAJUT DAN RPH BATURRADEN BANYUMAS Nadi Destaranti; Sulistyani Sulistyani; Edy Yani
Scripta Biologica Vol 4, No 3 (2017)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

A study on the undergrowth vegetation within the pine woods (Pinus merkusii) stands conducted in RPH Kalirajut and RPH Baturraden. This study aimed to find out the composition of the shrubs of the pine woods stands on those two sites with different altitude and to figure out the similarity of the herbs of the pine woods stands on those two locations with the different height. This study applied quadrat sampling technique, using 30 units of 2 x 2 m quadrats divided into ten sub–transects along the main transect. We measured environmental factors including elevation, temperature, light intensity, and pH of the soil. We found undergrowth vegetation in RPH Kalirajut comprised of 32 species belonging to 20 families, dominated by Ottochloa nodosa, Oplismenus compositus, and Cynodon dactylon. On the other site, we found undergrowth vegetation in RPH Baturraden composed of 19 species belonging to 20 families and dominated by Wedelia trilobata, Paspalum conjugatum, and Clidemia hirta. The similarity of the herbs vegetation measured of those two sites was 30.85% or 69.15% different.

PENGARUH PENAMBAHAN SERBUK KUNYIT (Curcuma domestica Vahl) TERHADAP MASA SIMPAN TEMPE KEDELAI Sherley Eka Destiana
Scripta Biologica Vol 6, No 1 (2019)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

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Tempe memiliki masa penyimpanan yang singkat dan cepat membusuk, proses pembusukan disebabkan oleh aktivitas mikroorganisme. Salah satu cara mencegah proses pembusukan dengan menambahkan kunyit. Kurkumin yang terkandung dalam kunyit bersifat antibakteri yang dapat menghambat pembusukan. Penelitian ini bertujuan untuk mengetahui pengaruh penambahan konsentrasi kunyit yang memberikan masa penyimpanan tempe lebih lama dan menciptakan cita rasa yang disukai konsumen. Pada penelitian ini ada 4 perlakuan yaitu penambahan kunyit dengan konsentrasi 0%; 0,5%; 1%; 1,5 %. Parameter uji yang digunakan antara lain total bakteri dengan metode Total Plate Count. pengamatan pertumbuhan miselia kapang, kekompakkan biji pada tempe dan uji organoleptik. Data yang diperoleh dianalisis menggunakan statistika sederhana (deskriptif). Hasil penelitian menunjukkan prosentase total bakteri terendah pada tempe kedelai tanpa kunyit dan kenaikkan total bakteri tertinggi pada tempe kedelai penambahan kunyit 0,5%, penambahan kunyit dengan konsentrasi 1 dan 1,5% menyebabkan penurunan prosentase total bakteri. Pengamatan miselia kapang tumbuh cepat, kompak pada tempe kedelai tanpa kunyit, penambahan kunyit menghambat pertumbuhan miselia dan miselia tumbuh lebih tipis dibandingkan miselia pada tempe kedelai. Tempe dengan penambahan kunyit konsentrasi 0,5%, 1% dan 1,5% lebih tahan lama daripada tempe kedelai. Menurut uji organoleptik dengan metode uji kesukaan, produk yang paling disukai adalah tempe kedelai dengan penambahan kunyit 0,5%. Serbuk kunyit menghambat pertumbuhan bakteri dan memperpanjang masa simpan tempe. KEY WORD: Tempe kedelai, kunyit, masa simpan.

KORELASI KARAKTER ANATOMI DAUN UBI JALAR (Ipomoea batatas L.) KULTIVAR TAHAN DAN TIDAK TAHAN TERHADAP INTENSITAS PENYAKIT KUDIS DAUN Ade Winda Pradana; Siti Samiyarsih; Juni Safitri Muljowati
Scripta Biologica Vol 4, No 1 (2017)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Sweet potato (Ipomoea batatas L.) is one of alternative food sources beside than rice. Sweet potatoes are contains minerals, nutrients, sources of energy, protein, vitamins A and C. Sweet potatoes have lower productivity than rice and cassava. The low productivity of sweet potatoes due to several factors, which one of them is a leaf scab disease caused by pathogens Sphaceloma batatas Saw. The purpose of this study is to determine the character of the anatomy of sweet potato leaf cultivars that resistant and unresistant to the intensity of leaf scab disease, as well as the correlations between the anatomy character of sweet potato leaves with leaf scab disease intensity. The method used in this study is an experimental method with factorial completely randomized design. The first factor is the character of the anatomy of four cultivars sweet potato leaves, Cangkuang and Sukuh cultivars (cultivars resistant), cultivars Cilembu and Beta (unresistant cultivars). The second factor is the inoculation treatment S. batatas Saw. The parameters were observed thickness of cuticle, epidermis, mesophyll, the size (length and width) stomata, density of stomata and trikomata as well as the intensity of the disease were analyzed using analysis of variance (ANOVA). To determine the correlation between the anatomy character leaves with disease intensity using regression correlation analysis. The results showed that Cangkuang cultivar has cuticle, epidermis and mesophyll thickest. Beta cultivars have stomata size of the longest and widest. Cilembu cultivar has the highest density of stomata. Sukuh cultivars has the highest density. The anatomy characters include of a thick cuticle, epidermis, stomata size (length and width), as well as the density of stomata and trikomata correlated with intensity of leaf scab disease.

PENGGUNAAN PESTISIDA DAN IMPLIKASINYA BAGI KESEHATAN PETANI DI KABUPATEN TIMOR TENGAH SELATAN, NUSA TENGGARA TIMUR Rully Adi Nugroho
Scripta Biologica Vol 5, No 2 (2018)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

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Pesticides pose a risk to public health and environmental in agricultural areas. This study described pesticide use among farmers in Oebobo, Batnun, Tupan and Pollo villages, South Central Timor, Indonesia. Some 400 farmers were interviewed about pesticide use, knowledge and attitude, symptoms of intoxication, personal protective equipment and hygiene. The farmers had adequate knowledge about pesticide toxicity, but farmer’s awareness did not necessarily translate into action. Majority did not use appropiate personal protective equipment nor good hygiene when handling pesticides. Farmers in South Central Timor did not use the most hazardous pesticides (class Ia and Ib). However, use of class II pesticides and those of lower toxicity in combination with inadequate practice among farmers was found in the study areas. Expensive protective equipment and behavioral patterns, such as spraying without protective clothing and mixing pesticides with bare hands combine to produce several well-documented health problems. Therefore, alternative crop management systems which could help preventing the negative effects of pesticides on farmer health and the environment are needed. Training farmers in integrated pest management methods, use of proper hygiene and personal protective equipment when handling pesticides should be promoted.

PETUNJUK PENULIS dan DAFTAR ISI Romanus Edy Prabowo
Scripta Biologica Vol 4, No 2 (2017)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

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