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Scripta Biologica
Published by Universitas Jenderal Soedirman
ISSN : -     EISSN : 23553138     DOI : -
Core Subject : Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology
Scripta Biologica is a peer reviewed open access electronic journal published by the Faculty of Biology Jenderal Soedirman University, publishes research papers encompasses all aspects of biology, including; molecular biology, cell biology, and genetics; physiology and reproduction; ecology and conservation; biodiversity, evolution, taxonomy, and biogeography.
Arjuna Subject : -
Articles 176 Documents
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PEMBUATAN DAN UJI ANTIBAKTERI GEL ARANG TEMPURUNG KELAPA TERHADAP BAKTERI Staphylococcus aureus Kurniawan - Kurniawan; Aisyah Sabila Rosyada; Arif - Mulyanto; Kurnia Ritma Dhanti
Scripta Biologica Vol 10, No 3 (2023)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.sb.2023.10.3.1525

Abstract

Staphylococcus aureus is a Gram-positive coccus that is an opportunistic pathogen, namely as a normal flora and also as a pathogen that causes nosocomial infections. This bacterium is able to adapt and easily undergo mutations, giving rise to new strains that are resistant to antibiotics and pose a threat to human health. Efforts to find active ingredients that are effective against S. aureus continue to be carried out by utilizing coconut product waste, namely coconut shell charcoal, which contains active ingredients in the form of antimicrobial compounds. The material is extracted and made into a gel preparation to make it easier to use. The purpose of this study was to obtain the optimal coconut shell charcoal extract gel formula and determine its ability to inhibit the growth of S. aureus bacteria in vitro. This type of research is true experimental, consisting of five groups, namely positive control, negative control, and concentrations of 3%, 6%, and 9%. The extract was prepared in the form of a gel and tested for antibacterial activity using the diffusion method. The optimal preparation of coconut shell charcoal extract gel was achieved at a concentration of 9%, and the greatest antibacterial inhibition against S. aureus bacteria was also obtained at a gel with a concentration of 9%. The coconut shell charcoal extract gel formula with a concentration of 9% is the optimal gel and has the greatest antibacterial inhibition against S. aureus bacteria.Key Words: antibacterial test, coconut shell charcoal, gel preparation, Staphylococcus aureus 
KAJIAN ETNOBOTANI TUMBUHAN API-API (AVICENNIA) DI DESA PAGATAN BESAR KABUPATEN TANAH LAUT Nurwafa Rosyida; Mahrudin Mahrudin; Riya Irianti
Scripta Biologica Vol 10, No 3 (2023)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.sb.2023.10.3.1496

Abstract

Etnobotani merupakan ilmu tentang pemanfaatan keanekaragaman tumbuhan di alam oleh masyarakat lokal. Penelitian ini bertujuan untuk mendeskripsikan etnobotani tumbuhan Avicennia di Desa Pagatan Besar Kabupaten Tanah Laut. Metode yang digunakan yaitu metode deskriptif dengan menggunakan teknik snowball sampling. Hasil penelitian menunjukkan dari kajian botani tumbuhan Avicennia berhabitus pohon, akar nafas, batang monopodial bentuk bulat, daun tunggal, bunga majemuk, buah sejati tunggal dan terdapat tiga spesies: Avicennia marina, Avicennia alba dan Avicennia officinalis. Kajian farmakologi digunakan sebagai obat antifertilitas/KB. Kajian etnoekologi terdiri dari faktor biotik yang sesuai untuk menunjang pertumbuhan, keberadaan Avicennia sebagai pencegah abrasi, penahan gelombang dan penahan angin serta memiliki penyebaran populasi masih banyak pada fase pra reproduktif dan kategori tidak kritis. Pada kajian etnososioantropologi sebagai penanda air laut akan pasang. Kajian etno-ekonomi sebagai kayu bakar, cemilan, tutup botol kecap, arang dan objek wisata. Kajian etno-linguistik diberi nama api-api dikarenakan bersifat panas ketika terkena getahnya dan dimanfaatkan sebagai kayu bakar yang menghasilkan api.
ANALISIS VEGETASI POHON DAERAH TANGKAPAN AIR WONOSADI KABUPATEN GUNUNG KIDUL Fitriah, Umi Novita; Hadisusanto, Suwarno
Scripta Biologica Vol 9, No 2 (2022)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Water is a basic necessity for living things. The catchment area (DTA) is very important to be conserved to maintain water availability and prevent the exploitation of springs. Studies on water and land conservation in Beji Village, Ngawen Sub-district are still very limited, especially DTA outside Wonosadi indigenous forest area. This study aims to study structure and composition of tree vegetation in DTA Wonosadi, Gunungkidul. Determination of DTA analyzed by using ArcGIS 10.2 with overlay of Hydrogeology Map, Indonesian Earth Map (RBI), and QuickBird Image. Analysis of vegetation in the analysis using Microsoft Excel software. Tree vegetation analysis used 10 m x 10 m square plot (40 plots) on each vegetation density class of DTA. The results showed that area of DTA Wonosadi was 23.300.924 m2. S. macrophylla King has highest density overall. S. macrophylla King, Tectona grandis L and Tectona grandis L has the highest Importance Value Index (IVI) of each vegetation class. This research concluded that tree vegetation structure in DTA Wonosadi is dominated by poles growth form. Tectona grandis L. is species that has the highest INP, which potentially affect ground water availability and reduce water discharge.
UJI AKTIVITAS ANTIFUNGI EKSTRAK DAUN KEMANGI (Ocimum basilicum L.) TERHADAP DAYA HAMBAT Candida albicans SECARA IN VITRO PADA KANDIDIASIS VULVOVAGINALIS Funsu Andiarna; Mei Lina Fitri Kumalasari; Moch. Irfan Hadi
Scripta Biologica Vol 10, No 2 (2023)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.sb.2023.10.2.1003

Abstract

Basil (Ocimum basilicum L.) can be used as a treatment for vulvovaginal candidiasis because it contains essential oils where there are flavonoids, alkaloids, tannins and saponins which can inhibit growth and kill fungal cells of Candida albicans. The purpose of this study was to determine the antifungal activity of basil leaf extract (O. basilicum) with variations in the concentration of the solution to the inhibition of C. albicans in vitro in vulvovaginal candidiasis. This study was an experimental study with basil leaf extract samples with various concentrations of 6.25 mg / ml; 12.5 mg / ml; 25 mg / ml; 50 mg / ml; 100 mg / ml; control (+) by giving 2% ketoconazole and control (-) by giving 10% DMSO. The research method uses disc diffusion test. Phytochemical tests of basil leaves showed that the leaves contained flavonoids, alkaloids, tannins, and saponins positively. The inhibition of C. albicans is best in the extract of O. basilicum leaves with a concentration of 100 mg/ml. The greater the concentration of the extract given, the more inhibited power will be formed because the concentration of the bioactive components contained in the extract is higher. Key Words: Basil leaf extract, Candida albicans, Inhibitory power, Antifungal compounds
PENGENDALIAN Meloidogyne hapla PADA TANAMAN TOMAT (Solanum lycopersicum) MENGGUNAKAN AGEN BIOKONTROL JAMUR PEMERANGKAP NEMATODA Dinna Cinthia Pangestu; Liana Dwi Srihastuti; Eli Masni
Scripta Biologica Vol 10, No 3 (2023)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.sb.2023.10.3.1504

Abstract

AbstraCTRoot-knot nematode is one of the parasitic nematodes on plants that can cause losses in agriculture. Plant parasites are one of the obstacles to increasing the growth of tomato plants (Solanum lycopersicum L.). One of the plant parasites that causes the decrease in tomato plants is the root-knot nematode caused by Meloidygne hapla. Nematodes can infect plants through their roots and can live in tissues causing disruption of root work. The large number of losses in agriculture requires nematode controllers to overcome these nematode attacks. The use of chemical nematicides is widely used by farmers to overcome nematode attacks, but this can have a negative impact on the environment, so alternative nematicides that are environmentally friendly are needed. This study aims to determine the potential ability of the Nematode Trapping Fungus isolated from the Lau Kawar area as a biocontrol agent to press the attack of Meloidogyne hapla on tomato plants. In this study, a completely randomized design was used with 5 treatments namely K(-), K(+), KBF (Carbofuran), LK TS, and LK TK with 3 replications each for 35 days. In vitro assay of nematode-trapping fungi on free-living nematodes was carried out and the test results showed that LK TS isolate had the ability to reduce nematode numbers by 94.5% and LK TK isolate by 86.1% at 24 hours of observation. Based on the bioassay test results, the isolates LK TS and LK TK had the ability to act as biocontrol agents in suppressing the number of vermiform nematodes, root swelling, and root knots in tomato plant roots. LK TS isolate could reduce the number of root nodes by 78% and LK TK isolates by 57.5%. The use of Meloidogyne hapla in research is a nematode that has the lowest infectious power compared to other types of nematodes. There was no significant difference in the effect of nematode infection on the growth of tomato plants.  
KEPADATAN, MORFOMETRIK DAN POLA DISTRIBUSI UDANG MANTIS Harpiosquilla raphidea DI PERAIRAN ESTUARI KARANG GADING Pane, Safitri Febriani; Mulya, Miswar Budi; Barus, Ternala Alexander
Scripta Biologica Vol 10, No 3 (2023)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Mantis shrimp, Harpiosquilla raphidea, an economically important commodity in Karang Gading, Deli Serdang, is at risk of population decline owing to uncontrolled fishing. This study was conducted to analyze population density, morphometric characteristics, distribution patterns, and correlations between physicochemical parameters and mantis shrimp density. The research was conducted in the Karang Gading wildlife sanctuary of Deli Serdang from July to September 2021, with sampling performed at three stations chosen by purposive random sampling. The total number of mantis shrimp individuals caught was 66, with a density of 4,83 ind/m2, on station 2 of 3,83 ind/m2, and station 3 of 2,33 ind/m2. Morphometric characters that have a strongly correlated effect were found in station 2 (total length with carapace length), a weakly correlated was obtained at station 1 (total length with carapace length), station 2 (total length with abdominal width, total length with telson width and total length with propodus length), station 3 (total length with carapace length, total length with abdominal width, total length with telson width and total length with propodus length) and moderately correlated was obtained at station 1 (total length with abdominal width, total length with telson width and total length with propodus length), all relationships between morphometric characters showed negative allometric growth patterns. The relationship between the total length and weight was obtained by the value of b < 3, which is a negative allometric. The distribution patterns at each station were categorized as grouped and unified, with an Id value of 0,791 – 1,004. The analysis results that strongly correlated and valued positively were salinity, current speed, and Dissolved Oxygen (DO); those with strong correlation and negative values were temperature, water brightness, and BOD5; moderately correlated and valued positively were pH and nitrate; strongly correlated and negatively valued (water depth); and weakly correlated and negative (phosphate).

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