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Scripta Biologica

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Published by Universitas Jenderal Soedirman

ISSN : - EISSN : 23553138 DOI : -

Core Subject : Science, Agriculture,

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

Scripta Biologica is a peer reviewed open access electronic journal published by the Faculty of Biology Jenderal Soedirman University, publishes research papers encompasses all aspects of biology, including; molecular biology, cell biology, and genetics; physiology and reproduction; ecology and conservation; biodiversity, evolution, taxonomy, and biogeography.

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Articles 176 Documents

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PENGARUH PEPTON TERHADAP PERTUMBUHAN EMBRIO ANGGREK Vanda tricolor Lindley var. Suasiv ASAL MERAPI SECARA IN VITRO Rizka Riliant Puspasari; Ikhsanudin Nur Rosyidi; Eka Fitriana Candra Ningrum; Endang Semiarti
Scripta Biologica Vol 5, No 1 (2018)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Orchidaceae is a valuable ornamental plant in Indonesia’s export commodities. The growth of orchid in nature is prolonged due to its microscopic size of the seed and has no endosperm. In the mass-production, an inducer is required to accelerate the growth in an in-vitro culture, one of which is peptone. The embryos used in this study is Merapi endemic orchid, the Vanda tricolor Lindley var. Suavis. This study aimed to determine the effect of peptone on the growth of eight weeks old of Vanda tricolor Lindley var. Suavis embryos and to determine the optimal peptone concentration to induce the growth of the orchid embryo. This experiment was conducted in the Biotechnology Laboratory of the Faculty of Biology Universitas Gadjah Mada from September to October 2016. The treatments applied in this study was a variety of peptone concentrations (0–3 g/L) in growth media to induce eight weeks old protocorm subcultures. The study measured the morphological development of protocorm to determine the effect. The results showed a positive effect of peptone on the growth of orchid embryos at the NP1 treatment concentration of 1 g/L. The growth reached the phase 6 with the rate measured about 6.85±0.001% in the fourth week. This value was higher than the protocorm growth rate when it reached the phase 6 in the fourth week on the NP0 medium measured at 5.22±0.003%, on the medium NP2 at 1.15±0.002%, and on the medium NP3 at 1.02±0.001%. This result showed the effect of peptone concentration to induce the growth of Vanda tricolor Lindley var. Suavis embryo, but the excess concentration of peptone inhibited the growth of protocorm.

KAJIAN ETNOBOTANI PADA TRADISI PERNIKAHAN ADAT KERATON SURAKARTA, PROVINSI JAWA TENGAH Eka Supriyati
Scripta Biologica Vol 5, No 3 (2018)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.sb.2018.5.3.758

Marriage is the emotional bond between man and woman to form a happy family. Marriage tradition including their ceremonial also conducted in areas such as the Keraton Surakarta. The ceremonial in Keraton Surakarta Central Java Province has been known as a basic pattern of tradition marriage by Javanese. Various types of plants are utilized in marriage tradition. The utilization of plants by community in particular areas is studied through the scince of ethnobotay. The purpose of this study is to know the wedding ceremonial tradition of Keraton Surakarta Indonesia in terms of the use of the plants and their meanings. This research used descriptive method with interviews. The sampling used purposive random sampling on servants in Keraton Surakarta and locals near Keraton. The result of the research shows the tradition of Surakarta traditional marriage utilizing various plants around it with a noble and positive meaning. The use of plants at each stage of marriage is different and has defferent meaning.

DEKOLORISASI LIMBAH BATIK MENGGUNAKAN LIMBAH MEDIUM TANAM Pleurotus ostreatus PADA WAKTU INKUBASI YANG BERBEDA Fitriya Yuni Wulandari; Nuniek Ina Ratnaningtyas; Ratna Stia Dewi
Scripta Biologica Vol 1, No 1 (2014)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

The batik waste industry is one source of water pollution. It must undergo treatment before discharge into the environment. Batik waste treatment technologies can be done biologically, chemically, physically, or combination of three processes. This research applied a combination of biology and physic technologies, namely used spent mushroom of Pleurotus ostreatus as the decolorization agent. Mycelium of P. ostreatus was used as biological agent, and cellulose from spent mushroom was used as physical agent. The aims of this research were to determine the effect of spent mushroom with variation incubation time on batik waste decolorization, and to know the optimum incubation time with highest decolorization batik waste percentage using spent mushroom of P. ostreatus. This research used experimental method with completely randomized design (CRD). The data obtained were statistically analyzed using Varian Test (ANOVA) on confidence level of 95% and 99% and continued with Honest Significant Difference Test (BNJ) with 95% confidence level. The result showed that variation incubation time treatment of batik waste decolorization used spent mushroom of P. ostreatus provide a highly signification effect on batik waste decolorization. The optimum incubation time was 60 hour (LW5), with decolorization percentage was 85,64%.

EFEK HIPOGLIKEMIK INFUSA DAUN TANAMAN MAKASAR, Brucea javanica (L) Merr, PADA TIKUS HIPERGLIKEMIK YANG DIINDUKSI ALOKSAN Handa Muliasari; Candra Dwipayana Hamdin; Muhsinul Ihsan
Scripta Biologica Vol 4, No 4 (2017)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Brucea javanica (L) Merr is a local plant used traditionally for diabetic medication in Lombok Island. The B. javanica seed has been recognized to have an antidiabetic effect, while the impact of the leave extract was understudied. The purpose of this research was to determine the effect of B. javanica leaves infusion on the blood glucose level of alloxan-induced diabetic rats. B. javanica leaves extract were applied by soaking leaves into hot water (95°C) to obtain the extract concentration of 5% (w/v). This experiment provides four treatments to rats within 15 days with five replicates for each treatment. The treatments for rats were K1 a positive control, K2 a negative control, K3 a healthy control, and P1 treated with leave infused water. The diabetic condition for K1, K2, and P1 was induced with an intravenous injection of 125 mg/kgBW alloxan monohydrate to the rats. After stable diabetic condition, K1 rats were treated with Glibenclamide (0,25 mg/kgBW), K2 rats were untreated, P1 rats were administered with leaves infused water of B. javanica (L) Merr 3 ml/day. K3 rats were uninduced and untreated. The hypoglycemic effect was observed by estimating the fasting blood glucose level of the rats every three days. After 15 days of treatments, P1 rats blood glucose level reduced by the 13,30%, while K1 rats 22,04%. The decrease in blood glucose level of both K1 and P1 rats have not reached the average blood glucose level of healthy rats. The result of the statistical analysis showed the infused extract effect was not significantly differed compared to positive control (P < 0.05).

TINGKAH LAKU MAKAN LUTUNG JAWA Trachypithecus auratus DI KAWASAN PANCURAN 7 BATURADEN GUNUNG SLAMET JAWA TENGAH Dwi Eliana; Erie Kolya Nasution; Indarmawan Indarmawan
Scripta Biologica Vol 4, No 2 (2017)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Javan Langur (Trachypithecus auratus) has an important ecological role in conserving the forest as their habitat by spreading seeds of fruits of their food. They are also responsible for the pattern of the plant diversity and forest regeneration as their habitat. Unfortunately, Javan Langur has been listed on Appendix II of CITES and categorized as vulnerable by the IUCN. Pancuran 7 Baturaden on the Mount Slamet slope is one of the few Javan Langur habitats in Java. Information on the Javan Langur in this area was inadequate. Therefore this study was aimed to get information on the type of food and the feeding behavior of Javan Langur, particularly in Pancuran 7 Baturaden. This study used a survey method, and the Animal Scan sampling technique was applied. The variables observed were sex ratio, population size, eating position, eating process, feeding locations, feeding duration, including the food type of the Javan Langur. Observed data for each variable were analyzed descriptively as percentages, and the results showed the Javan Langur were eating by sitting and standing, were marking trees to feed, were using hands and mouth to eat, were eating leaves and fruit, were eating on the trees and bushes. There were nine plant species as their food variety, while Matoa fruit and leave (Pometia pinnata) were their preferred foods.

PENGARUH AIR REBUSAN DAUN MAMPELAS (Tetracera indica Merr.) TERHADAP KADAR KOLESTEROL DARAH MENCIT Dian Samitra; Zico Fakhrur Rozi
Scripta Biologica Vol 4, No 3 (2017)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Tetracera indica Merr. locally known as Mampelas is commonly used in traditional remedies. It believed to have the potential to cure hypercholesterolemia. The potential use of this plant to lower blood cholesterol level was understudied; therefore this study aimed to determine the effect of the infusion of fresh leaves of Tetracera indica to the blood cholesterol levels of mice. This research was true experimental design, a post-test only design with nonequivalent (control) groups. This study used 25 male mice divided into five categories, i.e., P0 mice were administered with 0 g of infusion, P1 mice with 2 g infusion, P2 mice with 5 g infusion, P3 mice with 10 g infusion, and P4 mice without Tetracera indica infusion but 10 mg of simvastatin. The treatment in all categories were given continuously for seven days. The results showed the mean of the blood cholesterol levels were 169.4 ± 59.37 mg/dL in P0, 121.8 ± 7.62 mg/dL in P1, 136.2 ± 15.69 mg/dL in P2, 110.6 ± 37 , 01 mg/dL in P3 , and 179,0 ± 35,24 mg/dL in P4. Kruskal Wallis test was calculated to determine the difference of blood cholesterol level among all groups, and it reported p < 0.05. The results of this study showed that infusion of Tetracera indica leaf was significantly lowered the level of blood cholesterol in mice.

STRUKTUR KOMUNITAS DAN DISTRIBUSI TEMPORAL GASTROPODA DI DANAU SITU GINTUNG, TANGERANG SELATAN, BANTEN Yayan Mardiansyah Assuyuti; Alfan Farhan Rijaluddin; Firdaus Ramadhan; Reza Bayu Zikrillah; Dwi Cahya Kusuma
Scripta Biologica Vol 4, No 3 (2017)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

The changes in the physicochemical of waters in the lake ecosystem caused by seasonal variation, anthropogenic and industrial wastes impact the gastropod community. This study aimed to determine the physicochemical parameter of waters, community structure and distribution of gastropod, and the correlation between those two in the dry and rainy seasons. We conducted this research in the lake of Situ Gintung located in Tangerang Selatan, Banten, Indonesia during the dry season (May to August 2015) and the rainy season (February to April 2016). Statistical analysis showed the physicochemical parameter of waters in both seasons has no different, while the gastropod showed a slight difference in occurrence based on the season, in which rainy season had the highest occurrence. Canonical Correspondence Analysis (CCA) suggested that gastropods occurrence were affected by the light intensity in the rainy season and the temperature in the dry season.

KARAKTERISASI KROMOSOM UDANG GALAH (Macrobrachium rosenbergii) POPULASI SIRATU DAN MAHAKAM Diah Asta Putri; Nurul Suwartiningsih
Scripta Biologica Vol 6, No 2 (2019)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar

Giant freshwater prawn is one of Indonesia's freshwater prawn that has advantages such as fast growth and good meat quality. The continuous crossing of giant freshwater prawn that are closely related causes a decrease in its genetic diversity. In addition, the domestication process can also influences changes in the number and shape of chromosomes. This study is to determine the chromosome characters of domestication giant freshwater prawn (Siratu) and those derived from natural catches (Mahakam) which are cultured in the Unit Kerja Budidaya Air Payau Balai Budidaya Udang Galah (UKBAP BBUG) Samas. Research on the characterization of chromosome of giant freshwater prawn from both natural and domesticated populations needs to be done to obtain genetic information, especially chromosome characters. This information is useful to further support the process of breeding and for the certification process of giant freshwater prawn. Chromosome length measurements were carried out using the Image Raster 3 program. The shape of the chromosomes was determined by following the classification of Levan et al. (1994) based on the Sentromer Index Value obtained. The results showed that the number of chromosomes in the population of Siratu and Mahakam were 2n = 118. The giant freshwater prawn of Siratu population had the karyotype formula 2n = 118 = 94 m + 24 sm, while the giant freshwater prawn Mahakam population had the karyotype formula 2n = 118 = 118 m.

PERUBAHAN KADAR PROTEIN DAN STATUS LIPOSTATIK IKAN SIDAT, Anguilla bicolor, STADIA SILVER YANG DIPELIHARA PADA SALINITAS YANG BERBEDA Sintia Novia Lestari; Farida Nur Rachmawati; Untung Susilo
Scripta Biologica Vol 4, No 1 (2017)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Eel (Anguilla bicolor) is a catradomous fish, in the growth phase of life in freshwater and upon reaching adult will migrate to ocean waters to reproduce. Eel during the migration will use energy from body reserves to osmoregulation. Increased salinity will affect the osmotic pressure of the body of the fish so that the fish do active transport which requires energy. Energy that used for osmoregulation approximately 20–50% of the total energy from carbohydrates, fats and proteins. Carbohydrates are the first source of energy that is used and then will be replaced by fat and protein as energy after carbohydrate depleted. Thus the energy utilization during osmoregulation will affect the changes in the composition of protein and fat content of the body. Fat content will affect the value of lipostatic fish is one of the growth parameters. The purpose of this study was to evaluate changes in the levels of protein and lipostatic eel (Anguilla bicolor) is maintained in a range of salinity. This research method is experimental research design with CRD, treatment in the form of maintenance medium salinity; 4 ppt (control), 15 ppt, and 30 ppt. Each treatment was repeated 5 times. Test fish used were obtained from the Village Pesanggrahan Cilacap with range size between an average weight of 395–920 g and an average length of 59–81 cm. The variables measured were the levels of protein and lipostatic eel. Protein content measurement was conducted using kjeldahl and the fat content by soxhlet. Research results showed that the eels were exposed to salinity 4 ppt, 15 ppt and 30 ppt no effect on protein content and body lipostatic eel. It can be concluded that the eel is able to adapt well in the range between 4 ppt salinity to 30 ppt.

BIRDS AT SEBYAR RIVER, ARANDAY, BINTUNI, WEST PAPUA Freddy Pattiselanno; Agustina Yohanna Setyarini Arobaya; Alfredo Ottow Wanma
Scripta Biologica Vol 5, No 2 (2018)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

The River Sebyar is known as the home for particular aquatic birds and the wintering ground for migrant birds. The mass transportation along the river most likely impacted by the presence of wildlife species including birds. The research investigated the presence of water birds along the river. The study was done at six villages along the River Sebyar at the Aranday District of Bintuni Bay Regency of West Papua. The methods involved direct observation to the study sites by canoe-transect line along the river length with the assistance of binoculars, taxonomic description guide, and daily diary updated. Twenty-three birds were observed during the survey. Ten out of 23 bird species recorded in this survey have been observed that consisted of five species of Ardeidae, four of Anatidae, and one of Laridae. The escalation of human activities along the riverside especially during sago harvesting and a busy boat traffics within the Bintuni Bay tended to decrease spaces for particular bird species that usually occupied the areas for roosting and foraging assemblages.

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