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INDONESIA
INDONESIAN JOURNAL OF MEDICAL LABORATORY SCIENCE AND TECHNOLOGY
Published by Universitas Nahdlatul Ulama Surabaya
ISSN : 26846748     EISSN : 26569825     DOI : https://doi.org/10.33086/ijmlst
Core Subject : Health, Social,
Health Professions Social Sciences
Contributions will be considered for publication in Indonesian Journal of Medical Laboratory Science and Tehnology (IJMLST) concern kind from research, involvement and theory to functioning matters, education and training. The very wide spectrum of its topics includes: dosimeter, instrument enlargement, specialized measuring techniques, epidemiology, biological effects (in vivo and in vitro) and risk and environmental impact assessments.
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Articles 97 Documents
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A Comparative Study of Mean Platelet Volume in Diabetic Population With and Without Vascular Complication Ajantha Swamy Vasudhevan; Dhivya Mohan Sumathi; Ashwath Kumar Chinnaraju Selvakumar; Rajabalaji Rajabalaji
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 5 No 1 (2023): Step up to strengthen the laboratory system and prepare for patients care
Publisher : Universitas Nahdlatul Ulama Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/ijmlst.v5i1.3465

Abstract

Diabetes mellitus leads to long-term complications involving multiple organs and systems of the human body. Among the list of complications, a relatively vascular complication increases the morbidity of the condition. Patients with diabetes mellitus are influenced by various factors like hyperglycaemic state, insulin resistance, oxidative stress, and metabolic condition like obesity, and all the mentioned conditions also present with platelet hyperactivity.  Mean platelet volume (MPV) can be used as one of the laboratory parameters to know the function and activation of the platelets, which reflects the vascular profile of the patient. So, the present study compares the values of mean platelet volume among the diabetic groups to determine the relation between the vascular complication and the mean platelet volume. This study was conducted with 90 participants, who were divided into three groups. Group A  is non-diabetics, group B is type 2 Diabetics, and Group C is type 2 Diabetics with vascular complications and MPV.  On analyzing the statistical mean value of mean platelet volume, group B's (type 2 diabetics) value was higher than group A's (non-diabetics) and statistically significant with a p – value of 0.001. Similarly, the mean value of group C (type 2 diabetes with complications) was higher than group B (type 2 diabetics) and statistically significant with a p – value of 0.049 in the diabetics with and without vascular complications. On comparing the MPV of different study groups, the MPV is higher in the diabetic group with complicatiosn compared with the diabetics without complications.
Incidence of Hepatitis B Surface Antigen (HBsAg) Infection and Its Relationship with Risk Factors at Janitors at Regional General Hospital dr. M. M. Dunda Limboto Gorontalo Indra Elisabet Lalangpuling; Zumrotul Abidah Dakio; Nikma Nikma; Dwi Setiyo Prihandono; Lendawati Lendawati
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 5 No 1 (2023): Step up to strengthen the laboratory system and prepare for patients care
Publisher : Universitas Nahdlatul Ulama Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/ijmlst.v5i1.3623

Abstract

Hepatitis B is an inflammatory liver disease caused by the Hepatitis B virus, which can be acute or chronic. The outer membrane protein of Hepatitis B Virus (HBV) is known as Hepatitis B Surface Antigen (HBsAg). Janitors are employees who are tasked with cleaning the hospital environment to keep it clean, because of the the dangers that exist in hospitals, such as disease transmission, can occur if the hospital environment is not kept clean. The purpose of this study was to describe the results and their relationship to risk factors of the Hepatitis B examination on janitors at the Regional General Hospital (RSUD) dr. Mansyoer Mohammad Dunda Limboto Gorontalo. This study used a descriptive method with an accidental sampling technique. The sample in this study amounted to 33 respondents who met the inclusion criteria. Specimen were examined using the rapid test method. The data were presented descriptively and statistically to see the relationship between work and the incidence of Hepatitis B infection. The results obtained were as many as 33 respondents by conducting an HBsAg examination, with the results obtained being 2 reactive people and 31 non-reactive people. The results of statistical tests on the relationship between infection status and age showed a p-value of 0.019 and the relationship between infection status and symptoms showed a p-value of 0.002. The study’s findings revealed that there was a substansial correlation between the respondents’ reported symptoms and their infection status (6% of respondents teste for Hepatitis B).
Asymptomatic Bacteriuria and Antibiogram of Isolates Among Diabetic Patients in Calabar, Nigeria Paul Inyang-Etoh; Etefia Etefia; Sonia Ejiofor
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 5 No 1 (2023): Step up to strengthen the laboratory system and prepare for patients care
Publisher : Universitas Nahdlatul Ulama Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/ijmlst.v5i1.3797

Abstract

Asymptomatic bacteriuria is the presence of bacteria in the urine of an individual without any symptom of urinary tract infection. This has been widely observed in diabetic patients and could be detrimental to their health if not effectively managed. This study was to identify the pathogens associated with asymptomatic bacteriuria among patients with diabetes and the antibiogram of those isolates in Calabar. Blood samples were collected for the determination of fasting blood sugar levels using glucometer. Bacterial isolations were done through urine culture and antibiogram were tested in all urine samples of the diabetic patients in this study. The prevalence of bacteriuria in this study was 26.0%. Participants aged, 41-50 years were highest both in blood sugar level (12.3+/-4.38mmol/L) and infection rate (37.5%, 18/48). Females had lower blood sugar (10.9+/-3.370mmol/L) than the males (11.3+/-4.46mmol/L) while the males had less infection rate (22.4%, 22/98) than the females (29.4%, 30/102). Escherichia coli, 46.2% (24/52) had the highest distribution while Proteus spp. 11.5% (6/52) had the least distribution. Ciprofloxacin was the most sensitivity (100%) while Amoxicillin was the most resistant (38%). There was a high prevalence of asymptomatic bacteriuria in this study. The most commonly observed organisms were Escherichia coli. Ciprofloxacin was the most sensitive antibiotics and there was a widespread antibiotic resistance in this study. It is therefore recommended that screening among diabetic patients for urinary tract infections, sensitization and strategies to promote effective drug usage be encouraged.
Lycopene Improves the Metformin Effects on Blood Glucose and Neutrophil Counts in Type 2 Diabetic Rats Medina Sianturi; Neni Susilaningsih; Heri Nugroho; Maria Suryani
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 5 No 1 (2023): Step up to strengthen the laboratory system and prepare for patients care
Publisher : Universitas Nahdlatul Ulama Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/ijmlst.v5i1.3865

Abstract

In patients with type 2 diabetes mellitus (T2DM), both innate and acquired immunity are weakened by hyperglycemia. Lycopene is one of the hydrocarbon carotenoids that has been widely studied for its powerful antioxidant and anti-inflammatory properties, furthermore act as hypoglycemic and immunomodulator. Herein, we investigated the effect of lycopene and metformin combination on fasting blood glucose (FBG) and neutrophil counts. The rats were divided randomly into six groups, each containing five rats. Group 1 consisted of normal rats (N) and group 2, T2DM (DM) rats, which were administered 0.5 mL of coconut oil; group 3 T2DM rats were administered 250 mg/kg of metformin in 0.5 mL of coconut oil; groups  4,5 and 6 rats were administered a combination of metformin 250 mg/kg with 10 mg/kg (DML-10), 20 mg/kg (DML-20) and 40 mg/kg (DML-40) of lycopene in 0.5 mL of coconut oil, respectively. Treatment was administered every day for 28 days. A model of T2DM rats was induced by a high-fat diet for two weeks combined with streptozotocin–nicotinamide. Data were analyzed with a one-way ANOVA test followed by the least significant difference (LSD) test. There were significant differences in FBG levels and the number of neutrophils in all groups. Lycopene combined with metformin had lower FBG concentrations and higher neutrophil counts compared to metformin monotherapy (p<0.001), and these observations were dose-dependent. Lycopene combined with metformin can improve blood glucose and neutrophil counts in rats with diabetes. The highest effect was observed in combination with lycopene at a dose of 40 mg/kg and metformin at a dose of 250 mg/kg.
Analysis of Urine Podocalyxin in Type 2 Diabetes Mellitus Subjects With and Without Diabetic Nephropathy Jusni Ekasari Pelu; Liong Boy Kurniawan; Yuyun Widaningsih; Alfian Zainuddin; Husaini Umar; Nurahmi Nurahmi; Theosobia Grace Orno
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 5 No 1 (2023): Step up to strengthen the laboratory system and prepare for patients care
Publisher : Universitas Nahdlatul Ulama Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/ijmlst.v5i1.3933

Abstract

Type 2 diabetes mellitus is the most common cause of diabetes, consist from about 85% of cases. Diabetic nephropathy is a complication of diabetes mellitus in the kidneys which can end up as kidney failure. Podocalyxin (PDX) is a protein expressed in kidney podocytes that is involved in various cancers, and is also essential for kidney development. The research design was carried out using observational and cross-sectional analytic methods with total participants of 25 DM with diabetic nephropathy and 25 DM without diabetic nephropathy with a purposive probability sampling technique. This research conducted at the Endocrine Polyclinic, Clinical Pathology Laboratory, Hasanuddin University Medical Research Center (HUM-RC) Laboratory, Hasanuddin University Hospital, Makassar. The results showed that the urinary PDX level in DM subjects with nephropathy were 1.160 ng/mL and DM without nephropathy were 0.167 ng/mL (p<0.001), the urine albumin/creatinine ratio (ACR) of DM subjects with nephropathy were 644.74 mg/ g and DM without nephropathy of 10.071 mg/g (p<0.001) and the correlation test results of urine PDX and urine ACR in DM subjects with nephropathy (r=0.510; p=0.001). This study concluded that there was a significant difference between urinary PDX in DM with and without diabetic nephropathy, there was a significant difference between urine ACR levels in DM with and without diabetic nephropathy, and there was a relationship between urinary PDX levels and urine ACR in DM subjects with diabetic nephropathy.
In Vitro Anticoagulant Activity of Crude Protease of Bacillus tequilensis HSFI-5 Ethica, Stalis Norma; Raharjo, Tri Joko; Zilda, Dewi Seswita; Hidayati, Nur
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 5 No 2 (2023): Combatting Bacterial and Fungal Infections: The Critical Role of Advanced Researc
Publisher : Universitas Nahdlatul Ulama Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/ijmlst.v5i2.3791

Abstract

obtained from the fermented intestine of Holothuria scabra (sand sea cucumber). Strain HSFI-5 had been reported to be able to produce proteases, which had shown several characteristics of an antithrombotic agent, i.e., fibrinolytic and clot-lysis activities. However, its anticoagulation activity test is yest to be done. This study aimed to determine the anticoagulant activity of the crude protease HSFI-5 in vitro. The study design was a completely randomized design with a sample size of 90 calculated using the Federer formula. The material used was crude protease from B. tequilensis in skim milk broth. Prothrombin time (PT), activated partial thromboplastin time (aPTT), and plasma recalcification time (PRT) were carried out to test the anticoagulant activity. Citrated platelet poor plasma samples were divided into positive control, normal control, direct examination with crude enzyme in volumes of 50 and 100 µL and pre-incubation at 37ºC for 5, 10, and 15 min with crude enzyme volumes of 50 and 100 µL. The data normality was tested with the Kolmogorov-Smirnov test and the different tests were analyzed by one-way ANOVA with the Post hoc LSD test. The results of one-way ANOVA both on PT, aPTT, and PRT examinations showed that there was a significant difference between the treatment groups (p<0.05). The longest results of PT, aPTT, and PRT are positive controls, and the shortest results are normal controls for PT, and 15’ 50 group for aPTT and PRT. It is clear that crude protease B. tequilensis HSFI-5 exhibits anticoagulant as well as thrombolytic action, raising the possibility that it could function as an antithrombotic drug.
Potential of Sappan Wood, Purple Cabbage and Beetroot Extract in Sperm Staining Mamay, Mamay; Ernawati, Ernawati; Nurisani, Astari
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 5 No 2 (2023): Combatting Bacterial and Fungal Infections: The Critical Role of Advanced Researc
Publisher : Universitas Nahdlatul Ulama Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/ijmlst.v5i2.3961

Abstract

Examination of sperm morphology is an essential criterion for evaluating male fertility. This examination can be done by staining sperm cells with various techniques to facilitate the visualization of sperm cells. Several other methods that can be used to evaluate sperm morphology are Eosin-nigrosin, AgNO3 staining, Papanicolaou, Diff Quick and Giemsa. However, using these synthetic dyes can harm the environment and water sources because they prefer to decompose. The natural dye derived from environmentally friendly plants expected to minimize hazardous waste. This study aimed to identify and compare the potency of several natural dyes derived from purple cabbage, sappan wood and beetroot, which have not been studied in sperm studies. Sperm obtained from 30 men in the campus area and underwent 3-5 days of abstinence from intercourse in preparation for sampling. The sperm stained with natural dye, and their quality compared with WHO standard Papanicolaou. Extracts of sappan wood, purple cabbage and beetroot are made with various mordant alum, ethanol and acetic acid compositions. The study's results showed that the presence of mordant increased the staining quality of sperm with sappan wood and purple cabbage extracts. The use of acid improves the quality of sperm staining with beet extract. There was no different between dyeing with beetroot extract in various solvent compositions and dyeing with Papanicolaou to color all sperm components (p> 0.05). The results showed that beetroot extract has a high potential to evaluate sperm morphology.
Antioxidant Capacity of Melatonin against Oxidative Stress Caused by Exercise-Induced Weight Loss in Rats Aci, Recai; Keskin, Adem
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 5 No 2 (2023): Combatting Bacterial and Fungal Infections: The Critical Role of Advanced Researc
Publisher : Universitas Nahdlatul Ulama Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/ijmlst.v5i2.4159

Abstract

Antioxidant supplements such as melatonin are used to prevent oxidative stress during exercise. This study aimed to investigate the antioxidant capacity of melatonin supplementation against oxidative stress induced by the weight loss observed during exercise. Forty Wistar Albino male rats were subjected to 10 days of jogging exercises. Two groups were formed based on weight loss. Two subgroups were created in each group. Melatonin (5 mg/kg) was administered to each subgroup in each group. The other subgroups were designated as control subgroups. Blood samples were collected after 10 d. Superoxide dismutase (SOD), total antioxidant status (TAS), glutathione peroxidase, melatonin, and malondialdehyde levels were analyzed in blood samples. SOD, glutathione peroxidase, TAS, and melatonin levels in the melatonin subgroup were higher than those in the control subgroup in the non-weight loss group. In contrast, the malondialdehyde levels were lower. Melatonin levels in the melatonin subgroup were higher than those in the control subgroup in the weight loss group. Conversely, the SOD and TAS levels were lower. In addition, there was a positive correlation between weight loss and malondialdehyde levels and a negative correlation with SOD, TAS, and melatonin levels. Melatonin (5 mg/kg) supplementation showed antioxidant capacity in exercise without weight loss, but was insufficient in exercise with weight loss.
Antibiotic Susceptibility Pattern of the Potential Pathogens of Ventilator Associated Pneumonia in the Endotracheal Tubes of Critically Ill Patients Khatri, Prabhat Kiran; Negi, Vikrant; Kishoria, Naveen; Mathur, Namit; Sharma, Vandana
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 5 No 2 (2023): Combatting Bacterial and Fungal Infections: The Critical Role of Advanced Researc
Publisher : Universitas Nahdlatul Ulama Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/ijmlst.v5i2.4622

Abstract

An endotracheal (ET) tube offers surface for potential pathogens to attach and produce biofilm. These potential pathogens are typically hospital flora with a broad range of antibiotic resistance. The study aimed to characterize the biofilm-producing flora in ET tube of critically ill patients. Following extubation, ET tubes were retrieved aseptically from 100 different patients and promptly transported in a sterile ziplock bag. Each ET tube was cut into three different sections; inner lumen was scraped out and inoculated on Blood agar, MacConkey agar, and Chocolate agar. Colonies produced on media were tested for antibiotic susceptibility testing by applying disc diffusion and Colistin minimum inhibitory concentration (MIC). Out of 100 ET tubes, monomicrobial growth was observed in 62, polymicrobial growth in 14, and no growth in 24 specimens. A total of 93 potential pathogens were isolated including 25 (26.89%) Acinetobacter species, 23 (24.73%) Klebsiella species, 15(16.12%) Pseudomonas species, 13 (13.98%) E. coli, 6 (6.45%), Staphylococcus aureus, 4 (4.3%) Coagulase Negative Staphylococcus species (CoNS), 2 (2.15%) Proteus species, 1 (1.07%) Enterobacter species and 4 (4.3%) Candida species. Imipenem and Colistin proved to be among the most successful antibiotics against gram negative isolates. Only 1 out of 25 Acinetobacter species was resistant to Colistin. Methicillin resistance emerged in two S. aureus and three CoNS strains. Microorganisms usually adhere themselves to the surface of ET tubes. They may act as potential pathogens for the onset of Ventilator Associated Pneumonia (VAP) and are resistant to commonly administered antibiotics in hospitals. A technique to reduce or prevent the risk of biofilm development is crucial.
Histopathological of Mice (Mus musculus) Liver Induced by Lead (Pb) Orally Kodariah, Liah; Efrida, Pakpahan Suyarta; Aditya, Nugraha; Nurzal, Zalzabila Rena
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 5 No 2 (2023): Combatting Bacterial and Fungal Infections: The Critical Role of Advanced Researc
Publisher : Universitas Nahdlatul Ulama Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/ijmlst.v5i2.4295

Abstract

Lead (Pb) is a prominent heavy metal emitted by motor vehicle exhausts, factory and mining fumes. Its presence in the atmoshpere can endure for up to seven days, posing a considerable risk of contaminating surrounding food and beverages. Lead enters the body through inhalation and the skin. Lead can also enter the human body via the oral route and accumulate in the body. It causes health problems such as oxidative stress and damage human organs such as the kidneys and liver. This research aims to examine the effect of oral lead exposure on the liver histopathology of Swiss Webster strain mice (Mus musculus). Employing a non-probability sampling technique, 25 male mice were divided into 5 groups: negative control, K2, K3, K4 and K5. These mice were administered a daily oral dose of 0.5 mL and subsequently euthanized in CO2 chamber the following week for liver dissection. The findings reveal signs of hydropic degeneration characterized by cellular swelling, irregular shapes, and disrupted organelles in groups K2, K3, K4, and K5. In addition, the mean degree of liver damage was observed as 0 for the negative control, 1 for group K2, 1 for group K3, 2 for group K4, and 3 for group K5. In conclsuin, this study confirms that lead exposure can result in dentrimenal liver histopathology changes in mice.

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