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INDONESIA
Indonesian Journal of Clinical Pathology and Medical Laboratory (IJCPML)
Published by Perhimpunan Dokter Spesialis Patologi Klinik Indonesia
ISSN : 08544263     EISSN : 24774685     DOI : https://dx.doi.org/10.24293
Core Subject : Health, Science,
Biochemistry, Genetics & Molecular Biology Health Professions Medicine & Pharmacology Neuroscience
Indonesian Journal of Clinical Pathology and Medical Laboratory (IJCPML) is a journal published by “Association of Clinical Pathologist” professional association. This journal displays articles in the Clinical Pathology and Medical Laboratory scope. Clinical Pathology has a couple of subdivisions, namely: Clinical Chemistry, Hematology, Immunology and Serology, Microbiology and Infectious Disease, Hepatology, Cardiovascular, Endocrinology, Blood Transfusion, Nephrology, and Molecular Biology. Scientific articles of these topics, mainly emphasize on the laboratory examinations, pathophysiology, and pathogenesis in a disease.
Arjuna Subject : Kedokteran - Kedokteran (Lain-Lain)
Articles 17 Documents
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Search results for , issue "Vol 20, No 2 (2014)" : 17 Documents clear
METODE BROMCRESOL GREEN (BCG) DAN BROMCRESOL PURPLE (BCP) PADA SIROSIS HATI YANG MENDAPAT INFUS ALBUMIN Miftahul Ilmiah; Leonita Anniwati; Soehartini Soehartini
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 20, No 2 (2014)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v20i2.1070

Abstract

Albumin infusion is one of the therapeutic options in hypoalbuminemia patients. Serum albumin can be used to determine the albumininfusion therapy, prognosis and monitoring of liver cirrhosis. The time difference in measurement of serum albumin by bromcresol green(BCG) and bromcresol purple (BCP) methods can give different results. Serum albumin examination was done in 20 sera taken fromcirrhosis patients. Serum albumin was then evaluated before treatment, one (1) hour and 24 hours after the patient received an infusionof albumin and examined by bromcresol green (BCG) and bromcresol purple (BCP) methods. The serum albumin level by BCG methodincreased with a coefficient of 0.12 (p-value=0.022) with BCG method before (1.94±0.32 mg/dL) and after one (1) hour (2.06±0.32mg/dL) receiving intravenous albumin. The coefficient of albumin levels before and after 24 hours (2.12±0.38 mg/dL) was 0.18 (pvalue=0.07), whereas the increased levels of serum albumin after one (1) hour and after 24 hours of intravenous albumin, were notsignificant (p-value=0.467). The BCP method showed that serum albumin before, after one (1) hour and after 24 hours receivingintravenous albumin were 1.68±0.36 mg/dL, 1.87±0.36 mg/dL and 2.12±0.63 mg/dL respectively. The albumin levels showed asignificant increase before and after one (1) hour infusion of albumin (p-value=0.00), both levels shown before and after 24 hours(p-value=0.001), as well as one (1) hour and 24 hours after receiving intravenous albumin (p-value=0.04). The results of this studyshowed that increased serum albumin by BCG method could be detected after 1 (one) hour, whereas by BCP method could only be detectedafter 24 hours receiving intravenous albumin.
KEABSAHAN ENGRAILED-2 DI KANKER PROSTAT Elsa Yulius; Ida Parwati; Anna Tjandrawati; Dewi Kartika T
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 20, No 2 (2014)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v20i2.1084

Abstract

Prostate cancer is one of the most common malignancy of males found worldwide including in Asia, and is the second most commoncause of male death in Western countries. Specific cause of prostate cancer is not yet elucidated, but there are several risk factors as well.The diagnosis of prostate cancer is confirmed by digital rectal examination (DRE), prostate-specific antigen (PSA), and a biopsy withtrans-rectal ultrasonography (TRUS). However, the sensitivity of DRE and PSA examinations are low and not good enough to detectprostate cancer. Currently there is a new test called Engrailed-2 (EN2) examination with enzyme-linked immunosorbent assay (ELISA)micro method. The test is simple and uninvasive one. This study is aimed to know the validity of urinary EN2 protein level measurementto detect prostate cancer by analysing. This study was conducted at Dr. Hasan Sadikin Hospital Bandung between November 2012 untilMarch 2013. This study was a diagnostic test with cross sectional design. The subjects of study were men whom suspected to have prostatecancer by DRE, PSA value (≥4 ng/mL) and TRUS biopsy. Urinary EN2 protein levels were measured using ELISA micro method. Statisticalanalysis used Mann-Whitney test, table 2×2 and ROC curve. In this study there are 50 subjects, where the result of positive biopsy forprostate cancer were found on 17 subjects and the negative were on 33 subjects. The study results on cut off value of >0.7 ng/mL gavethe sensitivity, specificity, positive predictive value, negative predictive value, and accuracy of 94.12%, 63.64%, 57.1%, 95.5% and 74%,respectively. In conclusion of this study, that the urinary EN2 protein level examination using ELISA micro method has the high sensitivityand moderate specificity, thus these procedure primarily can be used for the screening of prostate cancer.
RERATA VOLUME TROMBOSIT, HITUNG LEUKOSIT DAN TROMBOSIT DI APENDISITIS AKUT Jayadi Festiawan; Nurhayana Sennang; Ibrahim Abdul Samad
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 20, No 2 (2014)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v20i2.1075

Abstract

The diagnosis of acute appendicitis and its complications still depends on the radiological examination and laparoscopy techniques.The aim of this study was to know the Mean Platelet Volume (MPV), WBC and platelet value in the diagnosis of acute appendicitis,associated with the incidence of existing complications. This research was carried out by a retrospective study by taking secondary datafrom the medical records, including the results of routine blood tests of acute appendicitis patients with and without complications atthe Dr. Wahidin Sudirohusodo Hospital between May 2011− May 2012. The data obtained in the study were MPV, White Blood Cell(WBC) and platelet count which were then analyzed using independent T test. The results obtained from 44 samples were that MPVvalues showed a significant difference (p=0.02), while WBC (p=0.12) and platelet count (p=0.13) were statistically not significantlydifferent between patients with and without complications. In conclusion, it was found that acute appendicitis with complications hadhigher MPV average values than those without complications and although there were significant differences between them, these werestill within the normal values.
SARI ETANOL, ETIL ASETAT ALANG-ALANG (IMPERATA CYLINDRICA) TERHADAP SUPEROXIDE DISMUTASE (SOD) St Khaerunnisa; Sutji Kuswarini; Suhartati Suhartati; Lina Lukitasari; Ira Humairah; Reza Arta BN; Gwenny IP
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 20, No 2 (2014)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v20i2.1080

Abstract

Oxidative stress has been considered as a mechanism involved in the pathogenesis and progression of many diseases such as:hypercholesterolemia and atherosclerosis. The objective of this current study was to know the potential antioxidant role of ethanol andethyl acetate extract of reeds (Imperata cylindrica) by investigation in rats fed with a high-cholesterol diet. Imperata cylindrica is richin antioxidants and has a cholesterol-reducing effect. A group of twenty four male albino Wistar rats was divided into four subgroups: anormal diet group (K0), a high-cholesterol diet group (K1), the same diet with ethanol extract of Imperata cylindrica group (K2), and thesimilar diet with ethyl acetate fraction of Imperata cylindrica group (K3),. After the treatment period, the hepatic antioxidant enzymeactivity (Superoxide dismutase/SOD) was determined. The rats fed on high-cholesterol diet with supplementation exhibited a significantelevation in SOD activity (p<0. 05), 61.6±12.4% inhibition, compared to normal animals 37.44±12.2%. The treated animals withethanol and ethyl acetate extract of Imperata cylindrica showed a significantly reduced the level of SOD activity (p<0.05, 29.90±5.6%as well as a 33.49±7.4% inhibition). The level of SOD activity when restored was closer to that in normal animals, signifying reversalof the oxidative stress. The study results showed that the treatment with Imperata cylindrica positively changed the hepatic antioxidantenzyme activities in high fat-diet rats, and thus had potential hypocholesterolemic and antioxidant effects. Imperata cylindrica couldprotect against oxidative stress linked to atherosclerosis and also decrease the atherogenic index.
SEKUENS TERAWETKAN TERKAIT HIV-1 Efrida Efrida; Andani Eka Putra
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 20, No 2 (2014)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v20i2.1081

Abstract

Human Immunodeficiency Virus-1 (HIV-1) have the high levels of genetic variability (molecular variation) related to the mechanismof deletions, insertions, and especially as a recombination. The recombination between subgroups and their inter subtype HIV-1 producesa new virus strain causing a certain infection. Until now there were about 40 circulating recombinant form (CRF) and 100 uniquerecombinant forms (URF) worldwide. Various HIV-1 molecular variation cause pitfalls HIV detection due to the difficulty test designed todetect all HIV-1 strains. The purpose of this study was to know the determination of the molecular variation that can identify the HIV-1genome sequences which are conserved from local isolates of West Sumatera. The method used is a descriptive study on samples of patientssuspected of being infected by HIV-1 based on the antibody screening results using rapid HIV-1 test in the laboratory of Clinical Pathologyat Dr. M. Djamil Hospital Padang. The RNA isolation and amplification by RT-PCR was performed at the Microbiology Department,Faculty of Medicine of UGM. The amplicons were sequenced using the Sanger method and analyzed by Clustal W with sequence datafrom the HIV its database. The major subtypes of HIV-1 isolates derived from local West Sumatra is AE/B with four (4) isolates (57.1%),followed by subtype AE, 2 isolates (28.6%) and B, 1 isolates (14.3%). Most of the molecular variation in this study related to thesubstitution of one base followed by deletions and insertions. The protease showed a wide variation, which consists of 20–37 substitutionsand 1–3 deletions. p24 is a highly conserved gene, followed by gp120 and vpu. Based on this study, it can be concluded that the isolatesof HIV-1 recombination is the biggest proportion with conserved region found at p24 of protein group of the gag.
HUBUNGAN ANTARA UMUR, UMUR MULAI SAKIT, LAMA SAKIT DENGAN LED, CRP, DAS28-LED DI ARTRITIS REUMATOID J. Soeroso; FM. Judajana
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 20, No 2 (2014)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v20i2.1072

Abstract

Rheumatoid arthritis (RA) is a chronic inflammatory autoimmune disease affecting joints with a high disability and prematuredeaths. The successful treatment depends on the monitoring and control of the disease activities. Age, Age at onset and its duration arerelated to the dynamics of the related activities in RA. The objective of the study is to determine the associations between the age, theAge at onset and its duration with the related activity markers like ESR, CRP and DAS28-ESR in RA. A cross sectional study was doneon 44 patients suffering from RA obtained from the Rheumatology Outpatient Clinic at the Dr Soetomo Provincial Hospital, Surabaya.They were diagnosed using the American College of Rheumatology (ACR) Criteria 1987. Kruskal-Wallis tests were used to determine theassociations between age (years), age at onset (years), its duration (months) with ESR (mm/hour), CRP (n) and DAS28-ESR respectively.Analysis results of the Kruskal-Wallis test includes: The age for the high ESR, namely: age ≥20 vs. age <20, p=0.035; age ≥30 vs.age<30, p=0.002; age ≥40 vs. age<40, p=0.000; and age ≥50 vs. age<50, p=0.047. The age for the high DAS28-ESR score namely:age≥30 vs. age<30, p=0.020; and age ≥40 vs age<40, p=0.013. The disease duration for the high ESR namely: disease duration ≥96vs. Disease duration <96, p=0.044. The disease duration for the high CRP namely: disease duration ≥84 vs. disease duration <84,p=0.015 and disease duration ≥96 vs. disease duration <96, p=0.029. All ages at onset segments were not associated with the high ESR,CRP as well as with the high DAS28-ESR. In this study it can be concluded that the age of patients suffering from RA is associated withhigh ESR. The older the age of the patients the higher will be the ESR. Older age is also associated with the high DAS28-ESR. The longerthe disease duration will be related with the high ESR, as well as the high CRP. ESR may be considered as a practical tool for measuringthe disease activity in RA, mainly for outpatients.
PEMERIKSAAN CKMB DAN HS-TROPONIN T PADA PASIEN INFARK JANTUNG DENGAN PENINGKATAN SEGMEN NON-ST AK Salim; M Suryaatmadja; DA Hanafi
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 20, No 2 (2014)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v20i2.1086

Abstract

Acute Coronary Syndrome (ACS)1 is a typical chest pain symptom due to ischemic coronary insufficiency caused by blockage of acoronary artery thrombus. There are three different states: Unstable Angina (UA), Non-ST segment Elevation Myocardial Infarction(NSTEMI) and ST segment Elevation Myocardial Infarction (STEMI).1 Cardiac Troponin (cTn) is a myocardial ischemia marker.6 A clinicalcontext is very important for the interpretation of the value of cTn, the increase of cTn is not always due to Acute Myocardial Infarction(AMI). According to a research, a new cTn examination called ‘high sensitive troponin T’ (hs-cTropT) has a higher sensitivity than theprevious generations for the diagnosis of AMI.7 A 60- year- old female patient entered the ER of Cardiovascular Hospital Harapan Kitasuffering from chest pain since the day before. From the examination it seemed that the patient had a risk factor of dyslipidemia. Thelaboratory results showed normal routine hematology, CKMB , renal function, glucose and electrolytes, however, hs - cTropT and lipidprofile were shown above normal. The differential diagnosis of the symptoms shown was: UA/NSTEMI, with Congestive Heart Failure(CHF) grade II and overweight.
GENOTIPE DAN SUBTIPE VIRUS HEPATITIS B PENDERITA YANG TERINFEKSI KRONIK AKTIF Gondo Mastutik; Juniastuti Juniastuti; Ali Rohman; Mochamad Amin; Poernomo Boedi Setiawan
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 20, No 2 (2014)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v20i2.1077

Abstract

Chronic activivity of Hepatitis B Virus (HBV) infection can lead to liver cirrhosis or hepatocellular carcinoma. The objective of thisstudy was to know by analyzing the distribution of HBV genotypes and subtypes from hepatitis B patients suffering from chronic activehepatitis B infection in Surabaya. The HBV genotypes were determined by comparing the S gene sequences to those kept in the GeneBank. The phylogenetic tree was constructed by means of the unweighted-pair group method using arithmetic averages. Furthermore,the subtypes were deduced based on the prediction of amino acid residues 116 to 183 of HBsAg on multiple sequences alignment withClustalW2. This study involved 20 sera obtained from patients suffering chronic active hepatitis B infection. After PCR and sequencing,it was found that 13 samples could be used for sequence analysis. The results showed that all sequences were clustered into HBV genotypeB. The subtype adw2 was identified from 12 of 13 sequences, whereas one (1) belonged to ayw1. The subtype adw2 is most prevalent inIndonesia, namely in the islands of Sumatra, Java, South Kalimantan, Bali, Lombok, Ternate, and Morotai, while ayw1 is found in theislands of Nusa Tenggara and Moluccas. Based on this study, it was found that the patients with HBV subtype adw2 were from Surabaya, whereas with ayw1 was from Nusa Tenggara. It can be concluded that the HBV infected patients with chronic active hepatitis B inSurabaya have the genotype B with subtype adw2 which was originally from Surabaya, whereas, ayw1 was a patient originally fromNusa Tenggara.
KADAR TGF-β1 PLASMA DAN LIMFOSIT-T CD4+DI PENDERITA YANG TERINFEKSI HIV STADIUM I Alberthina Alberthina; Endang R; Erwin AT
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 20, No 2 (2014)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v20i2.1079

Abstract

HIV infection and AIDS have been spread throughout the world and the number of patients continues to increase from year to year.Indonesia is one of the countries with quite a high increase in the incidence of HIV and AIDS. The absolute number of CD4+T-lymphocytesand percentage in HIV-infected patients can be used to determine the stage of the disease, and progression of the disease, as well as topredict the onset of the opportunistic diseases. In certain circumstances sometimes it is difficult to determine clinically and the results ofthe absolute number of CD4+ T-lymphocytes and its percentage are still high. So the examination of TGF-β1 is necessary for predictingthe disease course in the patient, because the increase of the disease progress is also accompanied by the increased levels of TGF-β1. Thepurpose of this study is to know and to prove the existence of the correlation between plasma levels of TGF-β1 and the absolute numberpercentage of CD4+T-lymphocytes in stage I HIV-infected patients. The research was carried out by a Cross sectional observational study,the samples were derived from 41 stage I HIV-infected patients treated at the Outpatient Clinic of the Infectious Disease IntermediateCare Unit (UPIPI) in the Dr. Soetomo Hospital from January to May 2012. The examination of TGF-β1 plasma was performed by ELISAmethod, the number of absolute and percentage of CD4+T-lymphocyte were counted by immuno flowcytometry (BD FACSCalibur™). Theresults were statistically analyzed using a Pearson product moment correlation test. It was shown that the TGF-β1 plasma levels in stageI HIV-infected patients tended to increase, as well as the number and percentage of CD4+ T-lymphocytes which were also increased. Theresult of this study revealed that the number of CD4+T-lymphocytes which were less absolute and more than 200 cells /μL showed nocorrelation with the plasma levels of TGF-β1 in stage I HIV infected patients. However, there was a significant positive correlation betweenthe number of CD4+T-lymphocytes percentage with TGF-β1 plasma in stage I HIV infection.
KADAR KALIUM DI PACKED RED CELLS SIMPANAN Angeline Sutjianto; Asvin Nurulita; Fitriani Mangarengi
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 20, No 2 (2014)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v20i2.1083

Abstract

Blood transfusion, is not only transferring the blood cells to the recipient, but also transferring other components such as: glucose,lactate, and potassium. When the blood is stored, many alterations occur in its cmponents, particularly a decrease of adenosine5-triphosphate (ATP) and pH, hemolysis, and an increase of potassium levels as well. One of the transfusion complications that should beavoided is hyperkalemia due to the accumulation of potassium that leaks during the storage. However, hyperkalemia related to transfusiondepends not only on the potassium level in the blood unit, but also on its volume and the rate of its blood administration as well. Theaim of this study was to know the potassium levels in stored Packed Red Cell (PRC). A cohort study was done from May–July 2010. Theresearchers used 48 samples from 16 PRCs derived from 16 donors. The samples were drawn from the of PRC hose’s plasma that had beensealed. The potassium levels were measured on the first day, 10th day, and 20th day of the blood collection. The mean potassium levelon the first day was 3.79 mmol/L, 10th day was 12.22 mmol/L and 20th day was 19.77 mmol/L. Comparison of the potassium levelsbetween the first and 10th day, between first and 20th day, and between 10th and 20th day showed a significant difference (p=0.00). Theincrease of potassium levels in the PRC coincide with the storagetime.

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