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INDONESIA
Indonesian Journal of Clinical Pathology and Medical Laboratory (IJCPML)
Published by Perhimpunan Dokter Spesialis Patologi Klinik Indonesia
ISSN : 08544263     EISSN : 24774685     DOI : https://dx.doi.org/10.24293
Core Subject : Health, Science,
Biochemistry, Genetics & Molecular Biology Health Professions Medicine & Pharmacology Neuroscience
Indonesian Journal of Clinical Pathology and Medical Laboratory (IJCPML) is a journal published by “Association of Clinical Pathologist” professional association. This journal displays articles in the Clinical Pathology and Medical Laboratory scope. Clinical Pathology has a couple of subdivisions, namely: Clinical Chemistry, Hematology, Immunology and Serology, Microbiology and Infectious Disease, Hepatology, Cardiovascular, Endocrinology, Blood Transfusion, Nephrology, and Molecular Biology. Scientific articles of these topics, mainly emphasize on the laboratory examinations, pathophysiology, and pathogenesis in a disease.
Arjuna Subject : Kedokteran - Kedokteran (Lain-Lain)
Articles 1,328 Documents
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PENGUKURAN JUMLAH LIMFOSIT CD4 METODE PANLEUCOGATING PADA PASIEN TERINFEKSI HUMAN IMMUNODEFICIENCY VIRUS (HIV) Umi S. Intansari; Budi Mulyono; Usi Sukorini
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 19, No 3 (2013)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v19i3.420

Abstract

The standard method for absolute lymphocyte CD4 count is by the use of flow cytometric analysis, but due to the high costly matter,a simpler method and less costly fee of PanLeucogating dual platform is needed. The aim of this study is to know how to replace the single platform by PanLeucogating dual platform by determining the agreement between both methods and to test the proportion of patient who have CD4 <200 cell/ul measured by PanLeucogating and single platform method. A cross sectional study was carried out in the Departement of Clinical Pathology Faculty of Medicine Gadjah Mada University Yogyakarta. The inclusion criteria of the study are HIV/AIDS patients who their CD4 were measured. Absolute count of CD4 was using FACS Calibur measured with both PanLeucogating and single platform method. The bias, correlation, regression and limit of agreement between both methods were analyzed using Bland Altman analysis to decide whether the two methods are interchangeable. The result of the study revealed an excellent correlation between the two methods (r=0.996; y=- 0.906 + 0.0.987). Bland Altman analysis revealed bias=5 cells/uL; standard deviation=18.7 and Limit of agreement (LOA)=-31.6 – 40.75. The Mean Percentage Difference (MPD)=2%; dan%LOA=-7.96 – 12.5%. PanLeucogating method has a good agreement with the single platform method and can be used interchangeable. There was no difference of the proportion of patient who has CD4 <200 cell/ul measured by Panleucogating method and single platform.
EVALUASI AKTIVITAS TRANSAMINASE, DAN KADAR BILIRUBIN PADA PENDERITA VIRUS HEPATITIS B DAN C Yosepin .; Benny Rusli; Hardjoeno .
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 16, No 1 (2009)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v16i1.996

Abstract

Hepatitis virus replicates only in hepatocyte. In hepatocyte injury, transaminase enzymes which normally intracellularly will transform to the blood circulation. And there are variation of increasing transaminase activities in HBV and HCV. To evaluatetransaminase activities, and bilirubin level in patients with HBV and HCV. Study design was cross sectional of 76 patients with HBVand HCV, data from medical record at Wahidin Sudirohusodo Hospital, Makassar, from January 2006 until July 2008 period. Datawas analyzed with Mann Whitney test using SPSS. windows versi on 12. of 76 patients with HBV and HCV were found 59 Men and 17women, age 15–72 years old. In there were no statistical significance between transaminase activites, bilirubin direct,indirect (p > 0.05),but there was significance difference of total bilirubin between HBV and HCV (p < 0.05). There were no statistical difference of AST,ALT, bilirubin direct and bilirubin indirect between HBV and HCV. Injury hepatocelluler because of HBV and HCV can not be identifiedjust by transaminase activities and bilirubin level, but there are other factor that influence.
SPONTANEOUS PLATELET AGGREGATION IN THIRD TRIMESTER PREGNANCY AT ADAM MALIK HOSPITAL, MEDAN Rezqi Maulani Jusuf; Hotma Partogi Pasaribu; Herman Hariman
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 25, No 1 (2018)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v25i1.1489

Abstract

              Spontaneous Platelet Aggregation (SPA) is a condition when spontaneously platelet aggregate in plasma left in a tube at room temperature for 2-3 hours without adding any agonists. It is not entirely sure why this phenomenon happens. Pregnant females have a more tendency of hypercoagulability. Nevertheless, SPA demonstrates hyper aggregation of platelet and not the coagulation pathway. So far, there is no report regarding this phenomenon in pregnant females. This study aimed to find out whether pregnant females, especially in the third-trimester of the pregnancy, had the tendency of SPA. This cross-sectional study was established in the Department of Clinical Pathology and Department of Obstetrics-Gynecology, Adam Malik General Hospital in Medan between January–March 2016. Twenty-six females were included. Ultrasonography (USG) was used to know the age of pregnancy. From 26 females in this study, 10 were pregnant females in the third-trimester of pregnancy while 16 others were normal non-pregnant females as controls, with a mean age of 29.2±2.78 years old and 36.5±4.63 years old. There were significant differences between platelet aggregation using ADP agonist in pregnant females and controls (p <0.01). In the median Tmax 10%, there were no significant differences between platelet aggregation without ADP agonist in pregnant females and controls after 3 hours (p >0.05). Hyper aggregation and hypercoagulability in the third-trimester pregnancy tend to develop SPA. Even though there are only mild signs and symptoms, it can be an arising risk of thrombus.
IDENTIFICATION OF DENGUE VIRUS SEROTYPES AT THE DR. SOETOMO HOSPITAL SURABAYA IN 2016 AND ITS CORRELATION WITH NS1 ANTIGEN DETECTION Jeine Stela Akualing; Aryati Aryati; Puspa Wardhani; Usman Hadi
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 23, No 2 (2017)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v23i2.1138

Abstract

Serotipe virus dengue yang beredar terus mengalami perubahan dan berbeda di setiap daerah. Pergeseran serotipe maupun genotipedi dalamnya, mempengaruhi terjadinya wabah dengue di berbagai negara. Perbedaan serotipe diduga bernasab dengan deteksi antigen(Ag) non-structural 1 (NS1), namun belum banyak penelitian yang mendukung hal tersebut. Penelitian potong lintang dikerjakan sejakFebruari-Agustus 2016 dan didapatkan 60 subjek infeksi virus dengue (IVD) dan 25 non-IVD. Ribonucleic acid (RNA) virus denguediperiksa di semua subjek menggunakan Simplexa Dengue Real-Time Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR)termasuk identifikasi serotipe virus dengue dan pemeriksaan NS1 menggunakan uji cepat NS1 Panbio. Perbedaan perbandingan variabelkategorikal dianalisis dengan uji Fisher Exact. Kenasaban antara serotipe dengan deteksi Ag NS1 dianalisis dengan Chi-Kuadrat. RNAvirus dengue terdeteksi di 43 dari 60 subjek IVD (71,7%). Serotipe terbanyak adalah DENV-3 (62,8%). Pergeseran dominasi serotipetelah terjadi di Surabaya, sebelumnya dari DENV-2 ke DENV-1 dan sekarang DENV-3, kemungkinan akibat mobilitas pejamu, transporvirus dan faktor geografis. Kepekaan uji cepat NS1 75% dan kekhasan 100%. Persentase deteksi NS1 antar serotipe berbeda bermakna(p=0,002). Deteksi NS1 lebih rendah pada DENV-1 dibandingkan DENV-2 (p=0,007) ataupun DENV-3 (p=0,003). Serotipe virusdengue bernasab dengan deteksi NS1 (p=0,005). Ciri serotipe maupun genotipe virus dengue kemungkinan mempengaruhi sekresiNS1. Telah terjadi pergeseran serotipe virus dengue di pasien IVD di Surabaya sehingga diperlukan surveillance berkesinambunganuntuk memperkirakan terjadinya wabah. Serotipe bernasab dengan deteksi NS1. Salah satu penyebab hasil negatif palsu NS1 adalahperbedaan serotipe.
GAMMA-GLUTAMYLTRANSFERASE DAN APOLIPOPROTEIN B DENGAN SINDROMA METABOLIK Tahono .; M.I. Diah Pramudianti
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 17, No 3 (2011)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v17i3.1056

Abstract

The number of Metabolic Syndrome (MS) patient has been rapidly increased. Diabetes Mellitus (DM) is a risk factor of coronaryheart disease that increased four fold in the general population. Gamma-glutamyltransferase (GGT) is located on the external surfaceof most cells and mediates the uptake of glutathione, which is an important component of intracellular antioxidants defenses. The aimsof this study was to analyze the correlation between GGT, Apolipoprotein B and metabolic syndrome parameters, a high sensitivityC-Reactive Protein (hs-CRP) as a marker of inflammation, and glutathione peroxidase (GPx) as an antioxidant marker in type 2 DMpatients. This study used a cross sectional design. The subject of this study was type 2 DM patients who came to the EndocrinologySubdivision of Internal Medicine Department and Laboratory of Clinical Pathology at Dr. Moewardi Hospital Surakarta. To analyze thisresult, the researchers used Spearman (r) correlation test with significance degree p < 0.05 and confidence interval 95%.The gammaglutamyltransferase serum showed a weak positive correlation with waist circumference (r = 0.18; p = 0.045) and Apo B with fastingblood glucose (r = 0.32; p = 0.001); a mild positive correlation between Apo B and triglyceride (r = 0.45; p = 0.001); a weak negativecorrelation between HDL cholesterol and GGT (r = –0.22; p = 0.020) and Apo B (r = –0.20; p = 0.027); a weak positive correlationbetween hs-CRP and GGT (r = 0.38; p = 0.001), and Apo B (r = 0.18; p = 0.042); and a weak negative correlation between Apo Band GPx (r = –0.22; p = 0.019). Gamma-glutamyltransferase examination is superior to notice any inflammatory process than apoB(r = 0.38 vs. R = 0.18), whereas apoB is superior as a predictor of inflammation than GGT (r = –0.51 vs. R = –0,20). Gammaglutamyltransferase serum as an early marker of oxidative stress and Apo B was related to inflammation at the metabolic syndromeand diabetes. Measurement of GGT serum is reliable, easy, and not expensive, it might have important implications both clinically andepidemiologically
BAKTERI AEROB PATOGEN DAN UJI KEPEKAAN ANTIMIKROBA DI RUANGAN PERAWATAN PENYAKIT DALAM Fedelia Raya; Nurhayana Sennang; Suci Aprianti
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 18, No 3 (2012)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v18i3.374

Abstract

Pathogenic bacteria are the major causes of airborne infection at the hospital ward. Nosocomial infection can occur at the opened as well as at the closed room. Nosocomial infection influences the morbidity and mortality in the hospital and need an extra attention, because of the increased number of hospital patients, micro organism mutation and increased of bacteria resistance to antibiotics. The aim of this study was to quantify the number of aerobic bacteria, and to know the pathogenic bacteria identification and its determination on the susceptibility of the antimicrobial problems at the internal medicine ward. This research was carried by a cross sectional study, which performed by collecting air samples in eight internal medicine ward of Dr. Wahidin Sudirohusodo Hospital using Microbiology Air Sampler 100 (MAS 100). The bacterial identification and the antimicrobial susceptibility test (AST) were conducted at the Balai Besar Laboratorium Kesehatan (July to August 2009). In this study were found the numbers of bacteria colonies about 580–6040 CFU/m3. The pathogenic bacteria that identified were Acinetobacter calcoaceticus, Staphylococcus saprohpyticus, Enterobacter hafniae and Stomatococcus mucilaginosus that were sensitive to Amikasin, Gentamicyn, Azitromycin and Norfloxacyn but resistant to Ampicillin. The number of bacterial colonies exceeded the established number standard by Decree of the Indonesian Health Minister. The pathogenic bacteria showed the most sensitive result of AST were Acinetobacter calcoaceticus, Enterobacter hafniae, Stomatococcus mucilaginosus and Staphylococcus saprohpyticus.
KADAR ALBUMIN SERUM PENDERITA STROK ISKEMIK DAN STROK HEMORAGIK Fasni Halil; Hj. Darmawaty ER; Ruland DN Pakasi
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 16, No 2 (2010)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v16i2.956

Abstract

To differentiate the ischemic and hemorrhagic stroke with the most accurate method can be carried out by Computerized Tomography(CT) scan. However, because the restrictor on access and cost, not all patients could gain the advantage of CT scan. Examination ofalbumin is a quick and easy test. The aim of this study was to evaluate the serum albumin level in patient suffering ischemic andhemorrhagic stroke. A cross sectional study of 60 ischemic and hemorrhagic stroke patients was performed at the Neurologic Departmentof Dr. Wahidin Sudirohusodo Hospital Makassar, from March up to August 2008. Albumin – levels was measured using the colorimetricBCG method, using the Cobas Integra 400 Autoanalyser. The data were than analyzed with SPSS 11.5 software and t test. Among the60 samples of the ischemic and hemorrhagic stroke were found mean values of serum albumin level is 3.38 ± 0.120 and hemorrhagicstroke is 3.51 ± 0.0938 with p value is 0.495. There were no significantly different between the serum albumin level of the ischemicand hemorrhagic stroke patients.
RELATIONSHIP BETWEEN SPECIFIC GRAVITY OF CUPRIC SULFATE AND SATURATION OF BLOOD DROPLETS DURING DONORS’HEMOGLOBIN SCREENING Resna Hermawati; Paulus Budiono Notopuro; Solichul Hadi
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 25, No 2 (2019)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v25i2.1376

Abstract

There are several methods of hemoglobin screening. A technique that is practical, easy, and fast,as well as does not require high costs is needed for blood donor activities. Cupric sulfate method is still used in blood donor activities nowadays. There are several types of the specific gravity of cupric sulfate which will determine saturation speed. On the other hand, one of the requirements for a blood donor, according to the WHO, is that its hemoglobin level must be ≥ 12.5 g/dL. This research aims was to reveal how many blood droplets could be dripped into cupric sulfate solutions at certain specific gravities. This research was an observational analytic study using a cross-sectional design. One mL of venous blood sample was consecutively collected from thirty donors who came to the blood service of the Dr. R. Soedjono Selong Hospital in August 2017. Next, screening for hemoglobin levels was carried out on those blood samples using the cupric sulfate method with a specific gravity of 1.054. Autoanalyzer was also used as a reference method in this research. Results of cupric sulfate examination were categorized into sinking and non-sinking. The results of cupric sulfate examination with sinking category showed significant suitability with hemoglobin levels of >12.5 g/dL from autoanalyzer (p=0.002) with 100% sensitivity and 70.83% specificity. The results of cupric sulfate examination with non-sinking category (with daily replacement) showed a significant match with hemoglobin levels of >12.5 g/dL from autoanalyzer (p=0.003) with 68% sensitivity and 100% specificity. The use of ten samples in this research could reach 100% sensitivity and 100% specificity. Meanwhile, according to the WHO, 30 mL of cupric sulfate solution with a specific gravity of 1.053 can accommodate 25 donor blood droplets.Thus, it can be concluded that unlike the reference from the WHO of 25 droplets, based on hemoglobin screening test cupric sulfate solution with a specific gravity of 1,054 can be saturated with ten droplets.
MULTIPLE MYELOMA IN A YOUNG ADULT (Mieloma Multipel di Dewasa Muda) Hendra Rasubala; Agus Alim Abdullah; Mansyur Arif
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 22, No 3 (2016)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v22i3.1248

Abstract

Mieloma Multipel (MM) merupakan penyakit tertentu di individu yang berusia lanjut. Kejadian mieloma multipel di pasien berusia dibawah empat puluh tahun adalah sangat jarang. Mieloma multipel merupakan kasus keganasan sel plasma yang mengenai banyak tulangdengan gejala peningkatan protein monoklon di serum/air kemih atau keduanya. Hal ini harus dibedakan dengan kasus peningkatanprotein monoklon yang lain seperti plasmasitoma soliter yang hanya terjadi di satu tulang. Dalam kasus ini dipaparkan pasien mielomamultipel laki-laki dewasa muda berumur 34 tahun, dengan hasil memeriksa laboratorik dan radiologis yang mendukung diagnosismieloma multipel tertentu. Keluhan utama berupa nyeri punggung yang disertai dengan kondisi tulang yang rapuh di gambaranradiologik. Pasien dirawat di rumah sakit selama tujuh belas hari di ruang perawatan bagian Bedah Ortopedi Rumah Sakit WahidinSudirohusodo, Makassar. Pasien meninggal pada hari ke-17 sesaat setelah menjalani kemoterapi pertama. Gambaran laboratorik yangmenonjol dan khas selama perawatan di rumah sakit seperti pemeriksaan hapusan aspirasi sumsum tulang, pemeriksaan elektroforesisserum protein, hematologik rutin, laju endap darah, zat kimia klinik (seperti kalsium, asam urat, uji fungsi hati, ureum dan kreatinin)dan pemeriksaan radiologik berupa foto polos tulang. Dalam makalah ini akan dipaparkan secara jelas, sampai akhirnya penyakit inididiagnosis beserta derajat dan perjalanan penyakitnya. Penanganan dan pengobatan pasien mieloma multipel selama perawatan inapdi rumah sakit juga akan dipaparkan dengan jelas dalam makalah ini.
CORRELATION BETWEEN IFN-ɤ LEVELS, CHEST RADIOGRAPHY AND THE POSITIVITY OF SMEAR SPUTUM IN NEW TB CASES AT THE DR.SOETOMO HOSPITAL Yessy Puspitasari; Jusak Nugraha
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 24, No 2 (2018)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v24i2.1316

Abstract

Tuberculosis is an infectious disease attacking lungs, triggering damage, and pulmonary dysfunction. Host cytokine responses will influence tuberculosis manifestations. The main host immune response is cellular immunity, and Delayed-Type Hypersensitivity (DTH). IFN-γ produced by Th-1, is a major cytokine acting to eliminate TB bacteria through macrophage activation. Chest radiography has an important value for the diagnosis of TB, especially in negative sputum smears. Radiological manifestations depend on several factors including host prior to TB exposure, age, and immune status. Sputum smear is also important in diagnosing, and assessing response to treatment of adult pulmonary TB. A cross-sectional study, comprising a total of 36 new pulmonary TB cases at the Dr.Soetomo Hospital who met the inclusion, and exclusion criteria, were establish. Plasma IFN-γ was examined by ELISA. Chest radiography was divided into three categories based on the National Tuberculosis Association of USA. Sputum smear data were taken from medical records. There were significant correlations between IFN-γ plasma levels with chest radiography (r= 0.365; ρ=0.029), IFN-γ with positive sputum smear (r= 0.447; ρ=0.006), positive sputum smear with chest radiography (r=0.674; ρ=0.001) IFN-γ plasma levels could reflect lesion area on chest radiography, and sputum smear positivity. IFN-γ plasma levels, chest radigraphy, and sputum positivity may reflect a Th-1immune response, so the more severe level of diseases, an immune response Th-1 become more activated. 

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