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INDONESIA
Indonesian Journal of Clinical Pathology and Medical Laboratory (IJCPML)
Published by Perhimpunan Dokter Spesialis Patologi Klinik Indonesia
ISSN : 08544263     EISSN : 24774685     DOI : https://dx.doi.org/10.24293
Core Subject : Health, Science,
Biochemistry, Genetics & Molecular Biology Health Professions Medicine & Pharmacology Neuroscience
Indonesian Journal of Clinical Pathology and Medical Laboratory (IJCPML) is a journal published by “Association of Clinical Pathologist” professional association. This journal displays articles in the Clinical Pathology and Medical Laboratory scope. Clinical Pathology has a couple of subdivisions, namely: Clinical Chemistry, Hematology, Immunology and Serology, Microbiology and Infectious Disease, Hepatology, Cardiovascular, Endocrinology, Blood Transfusion, Nephrology, and Molecular Biology. Scientific articles of these topics, mainly emphasize on the laboratory examinations, pathophysiology, and pathogenesis in a disease.
Arjuna Subject : Kedokteran - Kedokteran (Lain-Lain)
Articles 1,328 Documents
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KADAR Na, K, Cl PADA RAGAM (VARIASI) SELANG WAKTU PEMERIKSAAN SERUM Trisna Yustiani, Nyoman; Mutmainnah, Mutmainnah; DN Pakasi, Ruland; Hardjoeno, Hardjoeno
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 15, No 2 (2009)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v15i2.945

Abstract

The role of electrolytes in the human body is very important. Almost all metabolic processes are affected by electrolytes. The accuracyof examination results depend on the management in pre-analytical, analytical, and post-analytical processes. The management ofspecimen is one of the essential factors in pre-analytical process that may influence the accuracy of laboratory results. This study haspurposed to observe the influence of time-interval variation at the examination of Na, K, and Cl serum concentration. A cross-sectionalstudy was conducted on 30 outpatient’s serum samples who visited the Clinical Pathology Laboratory of Dr. Wahidin Sudirohusodohospital in Makassar during July to August 2007. The Na, K, and Cl serum samples were examined immediately, 1 hour-delayed, and2 hours-delayed. Data were analyzed using paired T test in SPSS version 11.5. In this study we found that there was no significantdifference for Na and Cl between immediate and delayed samples, similarly showed for K between immediate and 1 hour-delayed (p >0.05). A significant different for K between immediate and 2 hours delayed, and between the first hour and the second hour delayedsamples (p < 0.05). From this study we could conclude that delayed samples more than 2 hours had no influence for Na and Clexamination result. Delayed samples more than 2 hours for K had influence for the examination result.
CORRELATION BETWEEN LEVEL OF SOLUBLE FAS AND DEGREE OF SEPSIS SEVERITY BASED ON APACHE II SCORE Pauline Hadisiswoyo; Endang Retnowati; Erwin Astha Triyono
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 24, No 3 (2018)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v24i3.1402

Abstract

A widely used scoring system to assess the severity of sepsis is Acute Physiology, Age, and Chronic Health Evaluation (APACHE) II scoring system, however there are some disadvantages in using this. Other parameters are needed to predict severity and outcome of sepsis. Proinflammatory cytokines and Fas receptors are increased in sepsis and their concentration elevations are correlated with disease severity. An increase of soluble Fas level will follow increasing Fas receptors. This study aimed to prove any correlation between the level of soluble Fas and degree of sepsis severity based on APACHE II score. A cross-sectional observational study was conducted in January-June 2015 on 30 septic patients. APACHE II scores were calculated from the patients’physiological data, age, and chronic health problem status. Levels of soluble Fas were measured using the ELISA method (Human FAS/ CD95 (Factor-Related Apoptosis) ELISA Kit, Elabscience Biotechnology). Levels of soluble Fas ranged between 1,049-2,783 pg/mL (1,855.7 ± 477.27 pg/mL). APACHE II scores varied between 4-29 (17.2 ± 5.82). Significant positive correlations between levels of soluble Fas and APACHE II score (r=0.347, p=0.03) were found. A prediction model of soluble Fas levels based on APACHE II score was made. Linear regression analysis produced a prediction model of soluble Fas levels based on APACHE II score, in which soluble Fas level= 1,365.8 + 28.485 x APACHE II score.  
PLATELET DISTRIBUTION WIDTH VALUE AS A DIAGNOSTIC AND PROGNOSTIC MARKER IN ACUTE CORONARY SYNDROME Rahmi Rifany Latif; Liong Boy Kurniawan; Darmawati Rauf
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 24, No 2 (2018)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v24i2.1307

Abstract

An acute coronary syndrome is a form of clinical manifestation of coronary heart disease with atherosclerosis, and thrombosis processes. Platelets play an essential role in thrombosis. High PDW shows a variation of the morphology, and size of a platelet. This was a retrospective cross-sectional study comparing PDW, length of stay, and patient outcome in STEMI, NSTEMI, UAP, and control patients at the Dr.Wahidin Sudirohusodo Hospital Makassar during the period of January 2014 – December 2015. Data were analyzed using statistical software. One hundred, and seventy-two ACS patients were included; 65 patients had UAP, 48 patients had STEMI and 59 patients had NSTEMI. Sixty normal patients were included as a control. Kruskal-Wallis test showed a statistical difference in PDW between patients with STEMI, NSTEMI, UAP, and control (p=0.000). Post hoc test showed a significant difference between ACS, and control, STEMI, and control (p=0.000), NSTEMI and control (p=0.000), UAP, and control (p=0.000), but there no significant difference     between STEMI, and NSTEMI (p=0.320), STEMI, and UAP (p=0.980), NSTEMI and UAP (p=0.435). There was no significant difference in PDW between patients who survived, and died (p=0.298), and also patients with a length of stay of ≤ 7 days, and > 7 days (p=0.293). Platelet distribution width was higher in ACS patients compared with control patients, but could not be used to predict the outcome, and length of stay of patients with ACS. It is advisable to do further research with a large sample size to avoid bias.
COMPARISON OF FACTOR VIII ACTIVITY IN O AND NON-O BLOOD TYPES Adil Dinata Simangunsong; Yetti Hernaningsih
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 23, No 3 (2017)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v23i3.1197

Abstract

Golongan darah ABO telah dinilai untuk terjadinya berbagai proses penyakit. Penelitian terdahulu menyatakan tingginyakejadian trombosis pada golongan darah non O disebabkan tingginya aktivitas faktor VIII dibandingkan golongan darah O. Metodepenelitian merupakan analitik observasional secara potong lintang. Sampel darah sitrat dengan nilai APTT dan PTT normal tanpamempertimbangkan usia, jenis kelamin dan diagnosis. Aktivitas factor VIII diperiksa menggunakan sysmex CS 2100i. Analisis statistikmengunakan one way Anova untuk golongan darah A, B, O dan uji sampel T independen untuk golongan darah O dan non-O. Darikeseluruhan 30 sampel didapatkan 15 sampel golongan darah non-O dan 15 sampel golongan darah O. Pada golongan darah non-Odidapatkan 8 sampel golongan darah A dan 7 sampel golongan darah B. Terdapat perbedaan aktivitas faktor VIII yang tidak bermaknaantara golongan darah non-O dan O (p=0,277). Pada golongan darah A, B, O, juga didapatkan perbedaan yang tidak bermakna(p=0,108). Aktivitas faktor VIII pada golongan darah non-O lebih tinggi dibandingkan golongan darah O, tetapi tidak didapatkanperbedaan bermakna. Pada golongan darah A,B,O juga tidak didapatkan perbedaan bermakna. Mekanisme yang mendasari hubunganantara golongan darah ABO dengan aktivitas faktor VIII sampai sekarang belum diketahui. Tidak didapatkan perbedaan bermaknaaktivitas faktor VIII antara golongan darah O dan non-O. Diperlukan penelitian dengan jumlah sampel yang lebih banyak dan mengikutsertakan golongan darah AB.
PHOSPHATIDYLINOSITOL -3KINASE (PI3K) DI PERBENIHAN ADIPOSIT YANG DIPAJAN GLUKOSA TINGGI DENGAN RETINOL Novi Khila Firani; Bambang Prijadi
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 22, No 2 (2016)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v22i2.1111

Abstract

Retinol is one of the active forms of vitamin A. In the previous study, it was known that retinol level in serum of DM patient waslower than in healthy people, which correlated with an increase of the glucose levels in these patients. The importance of retinol in insulinsignaling mechanisms that play a role in the pathogenesis of DM is still unknown. One of the components that play a role in insulinsignaling on adipocytes is phosphatidylinositol-3 kinase (PI3K), which encourages the translocation of glucose transporter-4 (GLUT4) tothe cell surface. The aim of this study was to know the importance of retinol therapy in the levels of PI3K enzyme on visceral adipocyteculture with high glucose exposure (25 mM) as a model of DM in vitro by determination method. Retinol therapy was given at a doseof 0.1 μM, 1 μM , and 10 μM. Measurement of PI3K level was done by ELISA method. The mean (SD) levels of PI3K enzyme were 1.91(0.27), 0.94 (0.15), 1.98 (0.22), 1.69 (0.81), 2.04 (0.16) ng/mL respectively, for adipocyte cultures exposed to 5mM glucose (as aphysiological condition), 25mM glucose, and 25mM glucose concentration with doses of retinol therapy 0.1 μM, 1 μM and10 μM. Theresults of this study indicated that high glucose exposure (25 mM) decreased the level of PI3K compared with adipocyte’s culture on5 mM glucose exposure. Retinol therapy with a dose of 0.1μM, 1μM and10 μM on adipocyte culture exposed with high glucose couldincrease the levels of PI3K.
KADAR INTERLEUKIN-8 KANKER PAYUDARA Juranah Juranah; Yuyun Widaningsih; William Hamdani; Ruland DN Pakasi; Uleng Bahrun
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 20, No 3 (2014)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v20i3.471

Abstract

Metastasis is the major cause of increased morbidity and mortality in breast cancer. Interleukin-8 has a contribution in the spread of the tumour cells. This study was aimed to know IL-8 levels in patients with metastasic and non metasatic breast cancer by analyzing. A cross sectional study was performed at the Dr. Wahidin Sudirohusodo, Ibnu Sina and Labuang Baji hospitals during September to December 2011. Laboratory examinations were done in the Research Centre of the Medical Faculty, University of Hasanuddin. Fiftysix (56) subjects with breast cancer were examined. The results showed that the levels of IL-8 in metastatic and non metastatic breast cancer were 13.73 pg/mL and 10.48 pg/mL respectively. Mann-Whitney test showed no significant difference of IL-8 level between them, (p=0.09). When the subjects were divided into early and advance stages, the mean levels of IL-8 were 8.035 pg/mL and 12.052 pg/mL, respectively, showing a significant difference by Mann-Whitney test (p=0.009). Based on this research, the level of IL-8 can be considered as a marker in predicting the prognosis of breast cancer. A further research in cohort studies which specific metastasis crawl method is advised by the researchers, these are suggested to allow the determination of the risk factors.
PENGARUH (EFEK) KEMOTERAPI TERHADAP KERJA (AKTIVITAS) ENZIM TRANSAMINASE DI PENDERITA KANKER PAYUDARA Helena Leppong; Mutmainnah Mutmainnah; Uleng Bahrun
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 17, No 2 (2011)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v17i2.1024

Abstract

Breast cancer is one of the malignancies with unknown exact aetiology. The management of breast cancer can be done by severalways including it’s related chemotherapy. This therapy leads to cancer cells damage as well as healthy liver cells. Aspartate transaminase(AST) and Alanine transaminase (ALT) are the sensitive parameters to evaluate hepatocellular damage, the levels will raise rapidly inliver cells injury. The objective of this study was to evaluate the effect of chemotherapy in the activity of liver transaminase enzymes ofbreast cancer patients. A cross-sectional study was conducted by taking data from Medical Records of breast cancer patients who receivedchemotherapy at the Dr. Wahidin Sudirohusodo Hospital Makassar during the period of May 2008–May 2009. The data were analyzedstatistically by t-Test using SPSS software for Windows version 11.5. From 71 evaluated samples, the level of the liver transaminaseenzymes in all grades of cancer did not show a significant change (p>0.05). Chemotherapy of breast cancer patients does not increasethe activity of transaminase enzymes. In this study, the level of AST and ALT in chemotherapy patients can not predict the liver functiondisorder.
PERHITUNGAN JUMLAH SEL CD4 DENGAN SEROPOSITIF IgM HERPES SIMPLEKS TIPE-2 DI PASIEN HIV Bastiana Bermawi; Endang Retnowati K; Erwin A Triyono
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 15, No 3 (2009)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v15i3.970

Abstract

Herpes simplex type-2 (HSV-2) infection is usually transmitted sexually and can cause recurrent, painful genital ulcers. Seropositivityof IgM HSV-2 indicates acute infection of HSV-2. The low level of CD4 cell counts can lead to opportunistic infection in HIV patients.Therefore, the aim of this study is to investigate the association between CD4 cell counts and HSV-2 infection (based on seropositivity ofIgM HSV-2) in HIV patients with heterosexual risk factor in Dr. Soetomo Hospital, Surabaya. Specimens were collected during the firstpresentation on June and July 2008. HSV-2 IgM and was assessed by an ELISA technique using Herpelisa 2 IgM (recombinant) reagent,whereas CD4 cell counts was assessed by a Flowcytometry BD FACSCaliburTM System. The seropositivity of IgM HSV-2 in HIV persons18 years of age or older was 27.3% (9/33). Median CD4 cell counts the number were 50 cells in μl. Persons with seropositive HSV-2who had CD4 cell counts < 200 cells/μL and > 200 cells/μL were 66.6% (6/9) and 33.3% (3/9), respectively. Using chi square test,there was no association between CD4 cell counts and seropositivity of IgM HSV-2 in HIV patients. The seropositivity of IgM HSV-2 inHIV patients with heterosexual risk factor in Dr. Soetomo Hospital was 27.3%. There was no association between CD4 cell counts andthe seropositivity of IgM HSV-2 in HIV patients with heterosexual risk factor.
DIAGNOSTIC VALUE OF NEUTROPHIL-LYMPHOCYTE RATIO TO DIFFERENTIATE ISCHEMIC AND HEMORRHAGIC STROKE Martina Rentauli; Liong Boy Kurniawan; Darwati Muhadi
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 25, No 2 (2019)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v25i2.1446

Abstract

IntroductionStroke is a neurologic emergency disease and the main cause of high mortality. Inflammatory process in stroke due to cell and tissue damage causes increase of leucocyte prominently neutrophil. Neutrophil Limphocyte Ratio is an easy-to-measure inflammatory marker. There is only few data of RNL in Indonesia.MethodsThis was a retrospective cross-sectional study using secondary data from Medical Record RSUP Wahidin Sudirohusodo, Makassar. Leucocyte, neutrophil and lymphocyte first data were taken from adult  stroke diagnosed patients. Data statistically analyzed and diagnostic value of  NLR was determined by ROC curve analysis.Results dan DiscussionTotal of 402 patients were enrolled, 214 (54.72%) with ischemic stroke and 182 (46.8) with hemorrhagic stroke. There was a significant RNL difference between ischemic stroke (median 7.23) and hemorrhage stroke (median 3.65) (p<0.001). Using cut off 5.18, ROC curve showed of AUC 0.730 which had weak perfomance to differentiate ischemic and hemorrhagic stroke with sensitivity 67.8% and specificity 68.6%.Conclusion and SuggestionNLR in hemorrhagic stroke is higher than ischemic stroke. We suggest further studies with larger and more evenly distributed samples and consideration of sampling time. 
RANCANGAN PRIMER SPESIFIK GEN MACROPHAGE MANNOSE RECEPTOR (MMR) UNTUK POLYMERASE CHAIN REACTION (PCR) DAN SEKUENSING DEOXYRIBO NUCLEIC ACID (DNA) Yani Triyani; Nurizzatun Nafsi; Lelly Yuniarti; Nanan Sekarwana; Endang Sutedja; Dida Ahmad Gurnida; Ida Parwati; Bachti Alisjahbana
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 22, No 2 (2016)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v22i2.1120

Abstract

The order (sequencing) determinationof DeoxyribonucleicAcid (DNA) bases is the gene’s most basic information, using the methodof Polymerase Chain Reaction (PCR) as its stage. A key factor of successful detection by PCR is specific PCR primer design choice. Thedetection of diversity of Mycobacterium Mannose Receptor (MMR) gene, responsible for recognizing mannosylated antigen structureof Mycobacterium tuberculosis (M.tb) by DNA sequencing of exon 7 chromosome 10p12, related to susceptiblity for PulmonaryTuberculosis(TB), was first performed in China in 2012. The purpose of this study was to find specific primerfromboth design originatedfrom the research in China/primer I and my own design/primer IIby using Primer3 software. This study was based on 10 healthy subjectsand was a preliminary study of a research titled. The Relationship of Single Nucleotide Polymorphisms (SNPs) of Macrophage MannoseReceptor Gene to Pulmonary Tuberculosis Cases. The examination materials consist of 3 mL of EDTA blood and DNA extraction from itsbuffy coat. The resulting DNA was processed by PCR to amplify MMR gene with primer I and II. The primer I successfully amplified DNAfragments up to 780bp while primer II only 329 bp. The MMR gene DNA sequencing analysis was performed on the amplification resultof both kinds primers by using DNA Baser and Ensembl−BLAST software. The results were different, DNA sequencing result by using theprimer I was found in several chromosomes and also in several loci. Whereas, by using the primer II, it was only found in chromosome10 and in the same locus. Based on this study, it can be concluded that the specific primer design is one of the most important factorsin the success of DNA sequencing.

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