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INDONESIA
Indonesian Journal of Clinical Pathology and Medical Laboratory (IJCPML)
Published by Perhimpunan Dokter Spesialis Patologi Klinik Indonesia
ISSN : 08544263     EISSN : 24774685     DOI : https://dx.doi.org/10.24293
Core Subject : Health, Science,
Biochemistry, Genetics & Molecular Biology Health Professions Medicine & Pharmacology Neuroscience
Indonesian Journal of Clinical Pathology and Medical Laboratory (IJCPML) is a journal published by “Association of Clinical Pathologist” professional association. This journal displays articles in the Clinical Pathology and Medical Laboratory scope. Clinical Pathology has a couple of subdivisions, namely: Clinical Chemistry, Hematology, Immunology and Serology, Microbiology and Infectious Disease, Hepatology, Cardiovascular, Endocrinology, Blood Transfusion, Nephrology, and Molecular Biology. Scientific articles of these topics, mainly emphasize on the laboratory examinations, pathophysiology, and pathogenesis in a disease.
Arjuna Subject : Kedokteran - Kedokteran (Lain-Lain)
Articles 1,328 Documents
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MALARIA KONGENITAL Sri Wahyunie S; Nurhayana Sennang; D. Daud; Mansyur Arif
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 21, No 2 (2015)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v21i2.1109

Abstract

Congenital Malaria is an infectious disease caused by the malaria parasite that is transmitted from mother to child through theplacenta during pregnancy or at delivery. Clinical manifestations which may arise due to Plasmodium infection are: the irritability,fever, anaemia, jaundice and hepatosplenomegaly. The incidence of congenital malaria according to the National Basic Health Research2010 is only about 0.3%. Forty two days old male baby with the main complaints fever and pale since he was three (3) weeks old. Fromthe physical examination the reviewer found anaemia, jaundice and splenomegaly. Plasmodium vivax was detected by serologic andmicroscopic examination. From the pregnancy history of mother the reviewer found that at the age of seven (7) months of pregnancyshe suffered from malaria caused by Plasmodium vivax the same as the type of Plasmodium infected the baby. The baby was born innon malaria endemic area which enhanced the diagnosis of congenital malaria of this patient. The patient was fully recovered aftertreated with dehydroartemisin piperaquin and the reviewer reported one case of congenital malaria, forty twodays old male baby. Thediagnosis was made based on the malaria history of mother at seven (7) month of pregnancy, the serologic and microscopic examinationfrom the patient blood and the baby was born in a non malaria endemic area. The prognosis of patient with congenital malaria causedby Plasmodium vivax generally was good. The clinical condition was improved and fully recovered after treated with dehydro-artemisinpiperaquin.
CORRELATION BETWEEN PLATELET TO LYMPHOCYTE RATIO AND CORONARY ARTERY NARROWING Marziah, Enny; Aman, Adi Koesoema; Ketaren, Andre Pasha
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 24, No 3 (2018)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v24i3.1331

Abstract

Coronary Heart Disease (CHD) is one of the most common diseases of the cardiovascular system, characterized by atherosclerotic lesions. Atherosclerotic vascular processes are multifactorial. One of the factors is the process of inflammation. Platelet to Lymphocyte Ratio (PLR) is a marker that predicts the atherosclerotic coronary burden. The purpose of this research was to determine the correlation between platelet to lymphocyte ratio and coronary artery narrowing in CHD patients. This research was an analytical observation with a cross-sectional design, conducted on 54 patients with CHD who underwent angiography at the Adam Malik Hospital, Medan in February–October 2016. We examined platelet count, absolute lymphocyte count and percentage of coronary artery narrowing and coronary artery narrowing in CHD patients. There is a weak correlation between platelet to lymphocyte ratio and coronary artery narrowing in CHD patients ((r=0.276)). The result of the statistic test showed no significant correlation of platelet to lymphocyte ratio and coronary artery narrowing patients (p=0.043). In this study there is a weak correlation between platelet to lymphocyte ratio and coronary artery narrowing in CHD patients.
MOLECULAR ASPECT CORRELATION BETWEEN GLYCATED HEMOGLOBIN (HBA1C), PROTHROMBIN TIME (PT) AND ACTIVATED PARTIAL THROMBOPLASTIN TIME (APTT) ON TYPE 2 DIABETES MELLITUS (T2DM) (Aspek molekuler Hubungan Kadar Hemoglobin Terglikasi (HbA1c), Prothrombin Time (PT) dan Activated Partial Thromboplastin Time (APTT) di Diabetes Melitus Tipe 2) Indranila KS
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 23, No 1 (2016)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v23i1.1175

Abstract

Diabetes Melitus (DM) memerlukan pengendalian glikemia yang dapat diketahui dengan melakukan pemeriksaan hemoglobinterglikasi (HbA1c). Semakin tinggi kadar hemoglobin terglikasi (HbA1c), semakin tidak terkendali kadar gula darah pasien DM tipe2. Hal ini dapat menyebabkan terjadinya proses hiperkoagulasi dan gangguan mikrovaskular maupun makrovaskular. PemeriksaanProtrombin Time (PT) dan Activated Partial Thromboplastin Time (APTT) diharapkan dapat mendeteksi secara dini adanya gangguankoagulasi di pasien DM tipe 2. Penelitian potong lintang terhadap 72 orang pasien DM tipe 2 yang berusia diatas 18 tahun diperiksakadar HbA1c dan dikaji koagulasi (PT dan APTT). Pasien dengan penyakit penyerta seperti anemia dan kelainan hemoglobin, keganasanatau kelainan hematologis, pasca bedah, hipertiroid, perempuan hamil, riwayat penyakit hati dan pasien yang mengkonsumsi obatobatanyang mengganggu fungsi koagulasi dikeluarkan dari penelitian ini. Uji normalitas data menggunakan Kolmogorov-Smirnovdan analisis hubungan menggunakan uji Pearson. Analisis kenasaban terdapat hubungan antara kadar hemoglobin terglikasi denganProthrombin Time negatif lemah (r= -0,179; p=0,132) dan dengan Activated Partial Thromboplastin Time positif sangat lemah (r=0,016;p=0,892). Berdasarkan telitian ini terdapat hubungan negatif lemah yang bermakna antara kadar hemoglobin terglikasi dengan PTdan hubungan positif sangat lemah yang tidak bermakna dengan Activated Partial Thomboplastin Time.
ANALISIS TES IMUNOKROMATOGRAFI DAN ENZYME-LINKED IMMUNOSORBENT ASSAY UNTUK MENDETEKSI Helicobacter pylori DI PASIEN DISPEPSIA I Hutagalung; Uleng Bahrun; Mansyur Arif; Rifai Amirudin; HAM Akil
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 15, No 3 (2009)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v15i3.959

Abstract

Many methods are available to diagnose Helicobacter pylori in patients with dyspepsia, including Enzyme-Linked ImmunosorbantAssay (ELISA) and recently used is immnochromatography test. Both of the test are non invasive method, but immunochromatographytest can be used in laboratory without semi automatic analyzer. The aim of this study was to compare the immunochromatographyand ELISA tests in detecting the possibility of Helicobacter pylori infection. A cross sectional study was done among 49 sampleswith dyspepsia at Wahidin Sudirohusodo Hospital of Makassar and Prodia Laboratory from March to August 2008. Samples wereanalyzed with SPSS 14 for Windows Program using Chi Square and Spearman correlation tests. Among 49 samples we found ELISAand immunochromatography tests were positive in 5 samples, ELISA and immunochromatography tests were negative in 33 samples,ELISA tests were borderline and immunochromatography tests were positive in 5 samples, and ELISA tests were borderline andimmunochromatography tests were negative in 6 samples. There was a good correlation between ELISA and immunochromatographytest with R = 1.000 and p = 0.000. Both results of the immunochromatography and ELISA tests showed high conformity. Both methodcan be applied to diagnose Helicobacter pylori in patients with dyspepsia. Immunochromatography test can be an alternative methodin laboratories who do not apply semi automatic analyzer. The advantages of immunochromatography test can be used for diagnoseearly infection of Helicobacter pylori.
GENOTIPE DAN SUBTIPE VIRUS HEPATITIS B PENDERITA YANG TERINFEKSI KRONIK AKTIF Gondo Mastutik; Juniastuti Juniastuti; Ali Rohman; Mochamad Amin; Poernomo Boedi Setiawan
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 20, No 2 (2014)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v20i2.1077

Abstract

Chronic activivity of Hepatitis B Virus (HBV) infection can lead to liver cirrhosis or hepatocellular carcinoma. The objective of thisstudy was to know by analyzing the distribution of HBV genotypes and subtypes from hepatitis B patients suffering from chronic activehepatitis B infection in Surabaya. The HBV genotypes were determined by comparing the S gene sequences to those kept in the GeneBank. The phylogenetic tree was constructed by means of the unweighted-pair group method using arithmetic averages. Furthermore,the subtypes were deduced based on the prediction of amino acid residues 116 to 183 of HBsAg on multiple sequences alignment withClustalW2. This study involved 20 sera obtained from patients suffering chronic active hepatitis B infection. After PCR and sequencing,it was found that 13 samples could be used for sequence analysis. The results showed that all sequences were clustered into HBV genotypeB. The subtype adw2 was identified from 12 of 13 sequences, whereas one (1) belonged to ayw1. The subtype adw2 is most prevalent inIndonesia, namely in the islands of Sumatra, Java, South Kalimantan, Bali, Lombok, Ternate, and Morotai, while ayw1 is found in theislands of Nusa Tenggara and Moluccas. Based on this study, it was found that the patients with HBV subtype adw2 were from Surabaya, whereas with ayw1 was from Nusa Tenggara. It can be concluded that the HBV infected patients with chronic active hepatitis B inSurabaya have the genotype B with subtype adw2 which was originally from Surabaya, whereas, ayw1 was a patient originally fromNusa Tenggara.
CORRELATION OF COAGULATION STATUS AND ANKLE BRACHIAL INDEX IN DIABETES MELLITUS PATIENTS WITH PERIPHERAL ARTERIAL DISEASE Lany Anggreani Hutagalung; Adi Koesema Aman; Syanti Syafril
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 23, No 2 (2017)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v23i2.1139

Abstract

Diabetes Melitus (DM) sering dihubungkan dengan komplikasi mikrovaskular dan makrovaskular. Hiperglikemia merupakan faktorkebahayaan aterosklerosis dan penyakit vaskuler yang menyebabkan kerusakan pembuluh darah serta menyebabkan terjadinya glikasiterhadap hemoglobin, protrombin, fibrinogen dan protein lain yang terlibat dalam mekanisme pembekuan. Diabetes melitus merupakansalah satu faktor kebahayaan Penyakit Arteri Perifer (PAP). Tujuan penelitian ini adalah untuk mengetahui hubungan status koagulasiterhadap nilai ABI pasien penyakit arteri perifer dengan diabetes melitus. Penelitian ini bersifat analitik observasional yang dilakukandi Departemen Penyakit Dalam RSUP. Adam Malik Medan masa waktu bulan April-Oktober 2015. Pasien DM dilakukan pemeriksaanAnkle Brachial Index (ABI) dan status koagulasi seperti PT, APTT, fibrinogen dan D-dimer. Pada Penelitian ini menunjukkan perbedaanbermakna antara kadar fibrinogen dan D-dimer dengan PAP, yaitu kadar fibrinogen dan D-dimer di pasien DM dengan PAP lebih tinggidibandingkan dengan pasien DM non-PAP (333,35±127,49 vs 244,95±83,96; p=0,001) dan (648,40±443,96 vs 302,45±108,41;p=0,008). Didapatkan perbedaan bermakna antara kadar fibrinogen dan D-dimer dengan derajat PAP, yaitu kadar fibrinogen danD-dimer di derajat PAP berat lebih tinggi, dibandingkan dengan PAP ringan (374,00±114,94 vs 327,14 ± 136,45; p=0,012) dan (1170,67± 398,72 vs 537,36 ± 348,08; p=0,012). Setelah dilakukan uji kenasaban Spearman, Didapatkan adanya hubungan bermakna antarakadar D-dimer dengan PAP, yaitu peningkatan kadar D-dimer berhubungan dengan rendahnya nilai ABI (r -0,577; p=0,000). KadarFibrinogen dan D-dimer di pasien DM dengan PAP lebih tinggi dibandingkan di pasien DM non-PAP. Peningkatan kadar D- dimerberhubungan dengan rendahnya nilai ABI.
PERAN DOKTER SPESIALIS PATOLOGI KLINIK DALAM AKREDITASI RUMAH SAKIT (The Role For Clinical Pathologist in Hospital Accreditation) Anak Agung Wiradewi Lestari
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 21, No 1 (2014)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v21i1.1267

Abstract

The hospital accreditation of 2012 version assessment, performed through the evaluation of the implementation of hospitalaccreditation standards. It’s consisting of four (4) standards groups. The laboratory service is one component of the Assessment ofPatients (AOP) chapters. The role of Clinical Pathologist in this accreditation can be internal as well as external. In the laboratoryservices standard, there are 13 sections each with element of judgment. Some of the challenges are still encountered in the meeting forthe accreditation standards include the need for an agreement between the Clinical Pathologist and the Physician, perhaps even the roleof the Indonesian Association of Clinical Pathologist and Laboratory Medicine (IACPLM) to determine the values of which one has thecritical value, that should be reported by the laboratory to physician. In addition, most laboratories currently in Indonesia do not havea reference value corresponding to the population and geography. This would be the task of the specialist (us) together/IACPLM to beable to formulate the reference value or discuss it with the Hospital Accreditation Committee in order to obtain the same perception ofthe reference value. But considering the determination of the reference value is not a simple matter and this will require cost as well.Another challenge is controlling the laboratory equipment used in wards, such as Point of Care Testing (POCT) devices. Most POCTis used in wards is not provided by the laboratory. The hospitals should regularly receive reports and review the quality control of thereference laboratory. But so far, most of the reference laboratories do not report the results of quality control to the hospital laboratory.In one assessment standard, stated that the hospitals should have access to contact an expert of diagnostics specialist such concernedto parasitological, virological, or toxicological. The question is whether the Clinical Pathologist can not be regarded as an expert inparasitological and virological diagnostics?
EPIDEMIOLOGI DAN DIAGNOSIS KEDOKTERAN LABORATORIK INFEKSI VIRUS H1N1 Sembiring, J; Sianipar, O
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 16, No 3 (2010)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v16i3.1040

Abstract

H1N1 virus is an influenza virus family orthomyxoviridae, which clinical symptoms consist of: fever, cough, sore throat and headache. The virus can spread from person to person through airborne droplets and so a good infection control in the community or in healthworkers is needed, for instance by washing hands properly and PPE (Personal Protective Equipment). In October 4, 2009 there were over375.000 reported cases in which 4500 people were died. On June 11, 2009 the WHO stairs announced that H1N1 is the first pandemicin the 21st century. (The Indonesia people province was infecting by H1N1with 20 deaths until August 5, 2009). The purpose of thisarticle is to find out the epidemiological aspects as well as the diagnosis of influenza A H1N1 virus. From the website was found about1.110.000 sources, and after downloading them are selected as they are written only in the bibliography. From the searched informationobtained, it was known that H1N1 infection diagnosis begins with the discovery of heat (temperature ≥ 100° F [37.8° C]) and cough or sore throat. No cause other than influenza that is called with ILI was found. The informed laboratory examination is divided into three parts are confirmed if ILI was found as well as the virus in the throat or nasal swab by RT-PCR and/or virus culture; probably the ILIhas positive results of influenza A RT-PCR, but if the results are negative including the laboratorial findings, the basis of epidemiologyin this case was only suspected. Laboratory plays an important role in the establishing of the H1N1 infection diagnosis Rapid diagnosisis important to reduce infection, to protect patients from complications by antiviral treatment in the right time and also to provideimmediate information for the public.
KADAR ASAM URAT SERUM DAN KOMPONEN SINDROM METABOLIK MI Diah P; Banundari Rachmawati; Purwanto AP
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 20, No 3 (2014)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v20i3.469

Abstract

The incidence of metabolic syndrome (MetS) is significantly increasing. The elevated serum uric acid (SUA) level is correlated with the metabolic risk of cardiovascular. The relationship between SUA levels and components of MetS has been inconsistent. The aim of this study was to know the correlation between SUA level and component of MetS by analyzng. This study was a cross-sectional design conducted on healthy people at the Medical Check Up (MCU) Clinic, Dr. Moewardi Hospital, Surakarta from June 2012 until February 2013. MetS was defined according to the 2005 International Diabetes Federation (IDF) criteria. The statistical analysis was done by Student’s T test, Pearson or Spearman correlation coefficients. The researchers considered p<0.05 as statistically significant with a 95% confidence interval. A total of 80 healthy subjects, (range 19–57 years) were enrolled into this study. MetS was diagnosed in 40 persons where men (62.5%) were more than women and the non-MetS in women (62.5%) were more than men. In this study, a positive correlation was found between SUA and systolic blood pressure, diastolic blood pressure and waist circumference (Pearson r=0.354, 0.495, 0.399, respectively, p<0.05) in women, but not in men. The SUA was positively correlated with systolic blood pressure, diastolic blood pressure and waist circumference, particularly in women. A long term study is needed to investigate the consequence of increasing the SUA level in metabolic disorders and hormonal influences as well.
BIAKAN METODE TETRAZOLIUM MICROPLATE ASSAY TERKAIT DAHAK PASIEN TERDUGA TUBERKULOSIS PARU Rita Rachmayanti; Ida Parwati; Tiene Rostini; Sylvia Rachmayati
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 21, No 2 (2015)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v21i2.1088

Abstract

The definitive diagnosis of pulmonary tuberculosis is the discovery of Mycobacterium tuberculosis from sputum culture, but theconventional culture methods using Ogawa media require between 3−10 weeks detection time. Therefore it is needed a prompt diagnostictools to shorten the detection time. Tetrazolium microplate assay (TEMA) that used tetrazolium bromide as a growth indicator also usemitochondrial dehydrogenate enzymes in the mitochondria of living M. tuberculosis may reduce yellow tetrazolium bromide into purpleformazan crystals. The aim of this study was to know the validity and speed of time detection of M. tuberculosis growth by analyzingit. This study was carried out from November 2012 up to February 2013, which obtained 105 subjects conducted in the Departmentof Clinical Pathology at Dr. Hasan Sadikin Hospital with a cross sectional study design. The subjects consisting of sputum sample frompatients who suspected pulmonary TB which is examined for culture of M. tuberculosis with TEMA method using Ogawa media. Statisticalanalysis was used a 2×2 table to test the validity and Mann Whitney test for the differences in growth detection time. The validity testof TEMA method got the sensitivity of 90.4% and specificity of 96.2%. The detection time of M. tuberculosis growth in TEMA methodswas found fastest in the third day while from the Ogawa media cultur was found on the 13th day with the M. tuberculosis growth mediausing TEMA methods detected in 12 days. While for those cultured on Ogawa’s media the mean duration is 22 days (p<0.001). Basedon this study, can be concluded the examination of M. tuberculosis culture from sputum patient suffer of pulmonary TB with with TEMAmethod has given high validity and faster in the time detection for the diagnosis of pulmonary TB.

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