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INDONESIA
Indonesian Journal of Clinical Pathology and Medical Laboratory (IJCPML)
Published by Perhimpunan Dokter Spesialis Patologi Klinik Indonesia
ISSN : 08544263     EISSN : 24774685     DOI : https://dx.doi.org/10.24293
Core Subject : Health, Science,
Biochemistry, Genetics & Molecular Biology Health Professions Medicine & Pharmacology Neuroscience
Indonesian Journal of Clinical Pathology and Medical Laboratory (IJCPML) is a journal published by “Association of Clinical Pathologist” professional association. This journal displays articles in the Clinical Pathology and Medical Laboratory scope. Clinical Pathology has a couple of subdivisions, namely: Clinical Chemistry, Hematology, Immunology and Serology, Microbiology and Infectious Disease, Hepatology, Cardiovascular, Endocrinology, Blood Transfusion, Nephrology, and Molecular Biology. Scientific articles of these topics, mainly emphasize on the laboratory examinations, pathophysiology, and pathogenesis in a disease.
Arjuna Subject : Kedokteran - Kedokteran (Lain-Lain)
Articles 1,328 Documents
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KADAR INTERLEUKIN 10 (IL-10) MALARIA DAN ANEMIA I Nyoman Wande; Endang Retnowati; Juli Soemarsono
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 18, No 1 (2011)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v18i1.767

Abstract

Anaemia is an important complication of malaria, and its pathogenesis is not well understood. High level of the Th2 cytokine (such as IL-10), which counteract the Th1 cytokine, might prevent the development of severe malarial anaemia. The purpose of this study was to know the comparation between the plasma level of IL-10 in malaria patients with anaemia and without anaemia. The plasma level of IL-10 was examined in 16 malaria patients with anaemia and 16 malaria caused by P. falciparum patients without anaemia samplestaken from patients at the primary health centres in West Lombok and Centre Lombok during March until July 2008. The samples were measured using ELISA. The concentration of haemoglobin (Hb) was measured using hematological analyzer. The anaemia concentration of Hb is <11 g/dL. The results were analyzed using two (2) sample t test with SPSS ver.13.The plasma level of IL-10 in malaria patients caused by P. falciparum with anaemia was 8.81(3.04) [mean(SD)] pg/mL where as the plasma level of IL-10 in malaria patients without anaemia was 47.99(25.26) pg/mL. The mean of IL-10 level in malaria falciparum patients with anaemia was significantly lower than that of malaria patients caused by P. falciparum without anaemia (p=0.000).
THE COMPATIBILITY OF NEUTROPHIL TO LYMPHOCYTE COUNT RATIO WITH SERUM PROCALCITONIN AS BACTERIAL INFECTION MARKERS IN SEPSIS PATIENTS Elvinawaty Elvinawaty; Hanifah Maani; Zelly Dia Rofinda; Husni Husni
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 25, No 1 (2018)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v25i1.1492

Abstract

Bacterial infections and sepsis remain significant causes of morbidity and mortality in critical cases in Intensive Care Units (ICU) around the world. A quick and accurate diagnosis for sepsis is a challenge for clinicians and laboratory. The problem of an existing marker for sepsis with high sensitivity and specificity is still not satisfactory. Serum procalcitonin test is still widely used but cannot be supported by hospital laboratories especially in the regional public hospitals and needs more cost. Neutrophil-lymphocyte ratio is a potential index, easy, fast and cheap for bacteremia in patients with sepsis. This study aimed to determine the agreement of neutrophil-lymphocyte ratio with serum procalcitonin as a bacterial infection marker in sepsis patients. This study was an observational, analytical study with a cross-sectional design of 30 patients who had been diagnosed as sepsis by clinicians based on clinical signs of sepsis in the Intensive Care Unit of the Dr. M. Djamil Hospital Padang. The study was conducted from February to July 2016. Neutrophil and lymphocyte count was performed by flow cytometry method and procalcitonin test with Enzyme Linked Fluorescent Assay (ELFA). Results were analyzed by Kappa test with a statistical significance if p < 0.05. There were 30 subjects, consisting of 15 females (50%) and 15 males (50%) with the age mean of 48 years. Based on the Kappa test, the value obtained was kappa=0.634 (p=0.001) for the ratio neutrophil lymphocyte with procalcitonin. There was a substantial agreement between ratio neutrophil lymphocyte with procalcitonin test.
PNEUMATIC TUBE TERHADAP DARAH RUTIN DAN LAKTAT DEHIDROGENASE Liong Boy Kurniawan; Asvin Nurulita; Uleng Bahrun
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 21, No 2 (2015)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v21i2.1087

Abstract

The transportation of laboratorial samples with pneumatic tube system spends less time than when were handled by courier.Pneumatic tube system produces minor vibrations on sample due to the velocity changes during transportation and may cause changeson the blood cells and haemolysis. The aim of this study is to know the effect of sample transportation with pneumatic tube on bloodcells and its effect on hemolysis. A cross sectional study was performed at Dr. Wahidin Sudirohusodo Hospital, Makassar in July 2013.Routine blood, electrolyte and LDH were tested in 12 out-patients. The researchers collected two (2) samples, for each EDTA tube (routineblood tests) and serum (electrolyte and LDH). The samples were transported using pneumatic tube and the paired samples were sentby courier. The result then were analyzed with Paired T-Test. There were no significant difference of routine blood test results betweensamples sent by pneumatic tube and courier except RDW. RDW were higher in samples which were sent by pneumatic tube comparedto those brought by the courier (18.72±2.70% vs 17.83±2.36%, p=0.007). The electrolyte levels sent by both methods there were nosignificant difference, but the LDH levels were higher in samples sent by pneumatic tube (472.08±100.44 U/L vs 331.25±94.19 U/L,p=0.000). Based on this study, in common can be concluded that the pneumatic tube system does not effect on the routine blood testresults, except on RDW and does not cause changes due to haemolysis (on electrolyte) except the LDH levels elevates. So based on thisstudy, it is recommended to send samples for LDH test only by courier.
MODIFIKASI PRINSIP PEMERIKSAAN β-D-GLUCAN UNTUK MENDETEKSI CANDIDA ALBICANS DALAM SERUM Dharmawan, Ruben; Darukutni, Darukutni; Haryati, Sri; Murkati, Murkati; Sari, Yulia; Prasetyo, Afiono Agung
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 19, No 3 (2013)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v19i3.419

Abstract

Candida albicans β-D-glucans examination has been used for invasive fungal detection in human blood and was approved by the Food and Drug Administration. However, the method is rarely used in Indonesia and also because the cost is hardly affordable. A modification of the method using enzymatic reaction is hoped will provide a simple and affordable measurement in human blood serum. β-D-glucans as heterogeneous molecules constitute the major carbohydrates fractions of cell wall and readily detected in supernatants of Candida albicans cultures are hydrolyzed by β glucanase to form D-glucose. This additional glucose is measured using Megazyme GOPOD-Format Procedure® at 510 nm. Candida albicans were identified and cultured was derived from a patient of Dr. Moewardi General Hospital, Surakarta in July, 2012. The results show that β-D-glucans from Candida albicans is measureable to the amount of μg/100 μL serum using this modification principle
FACTORS IN ACUTE TRANSFUSION REACTION (Faktor Reaksi Transfusi Darah Akut) Wiwi Payung; Rachmawati A.M; Mansyur Arif
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 22, No 3 (2016)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v22i3.1245

Abstract

Data kejadian reaksi transfusi darah akut di Indonesia khususnya di Sulawesi Selatan belum pernah dilaporkan. Pencatatan danpelaporan tentang kejadian reaksi transfusi darah akut di RSUP Dr. Wahidin Sudirohusodo Makassar baru terlaksana mulai bulanJanuari 2014. Begitu pun dengan faktor yang mungkin berpengaruh terhadap kejadian reaksi transfusi darah akut belum pernahditeliti sebelumnya. Penelitian ini bertujuan untuk mengetahui faktor yang berpengaruh terhadap kejadian reaksi transfusi darah akut.Rancangan penelitian adalah kajian potong lintang. Sampel sebanyak 93 subjek, masa waktu antara bulan Januari–Juni 2014 di RSUPDr. Wahidin Sudirohusodo Makassar. Variabel yang diteliti antara lain diagnosis, riwayat transfusi darah sebelumnya, komponen darahyang ditransfusikan dan golongan darah. Metode menghitung yang digunakan adalah diskriptif dan analitik. Metode mendiskriptifkandilakukan dengan perhitungan sebaran kekerapan. Metode menganalitik dilakukan dengan menggunakan uji Chi Kuadrat dan MultipleLogistic Regression. Pasien yang mengalami reaksi transfusi darah akut ada 43 orang. Faktor yang berpengaruh adalah diagnosisp=0,765, riwayat transfusi darah sebelumnya p=0,563, komponen darah yang ditransfusikan p=0,046, golongan darah p=0,018.Berdasarkan kajian ini dapat disimpulkan, bahwa golongan darah merupakan faktor kebahayaan utama reaksi transfusi darah akut.Yaitu golongan darah O berkebahayaan 2,7 kali lebih besar untuk mengalami reaksi transfusi darah akut dibandingkan dengan golongandarah yang bukan.
BALANCE OF PROINFLAMMATORY AND ANTI INFLAMMATORY MARKERS IN ROUTINE HEMODIALYZED PATIENTS (A STUDY OF END-STAGE RENAL FAILURE PATIENTS AT THE DR. SARDJITO HOSPITAL YOGYAKARTA) Ira Puspitawati; Purwanto A P; Lisyani B. Suromo
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 24, No 2 (2018)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v24i2.1317

Abstract

Patients with End-Stage Renal Disease (ESRD) tend to have immune imbalance triggered by uremia and Hemodialysis (HD) procedures. Contact between dialysis membrane and blood will cause bio-incompatibility reactions inducing complement activation and production of Reactive Oxygen Species (ROS) as well as proinflammatory cytokines and acute phase protein such as C-Reactive Protein (CRP). Those immune response imbalances will lead to an immunocompromised condition. The objective of this study was to prove the correlation between inflammatory markers (IL-1β, IL-6 and C-reactive proteins) and its anti-inflammatory marker (IL-10) in routine hemodialyzed patients. This is a cross-sectional observational study involving 90 subjects conducted at the Hemodialysis Installation of the Dr. Sardjito Hospital. The inclusion criteria of this study were patients who underwent routine HD procedures, aged between 18 and 65 years-old, having leukocytes count and albumin level within normal limit. The exclusion criteria of this study were patients with Acute Coronary Syndrome (ACS) and malignancies. Levels of IL-1β, IL-6  and IL-10 were measured using Enzyme-Linked Immunosorbent Assay (ELISA), while CRP was measured using highly-sensitive CRP immunoturbidimetry. Statistical analysis was performed by Spearman test. This study results showed correlations between IL-1βand IL-10 (p=0.001, r=0.302), IL-6 and IL-10 (p=0.001, r=0.418) and correlation between CRP and IL-10 (p=0.005, r=0.295). There were also correlations between IL-1β and IL-6 (p=0.029, r=0.232), IL-6 and CRP (p=0.001, r=0.534), but no correlation was found between IL- 1β and CRP (p=0.431, r=0.073). All factors that trigger the secretion of proinflammatory cytokines will trigger the release of anti-inflammatory cytokines, the consequences of anti-inflammatory cytokine secretion will happen minutes after the release of inflammatory cytokines. This study showed that there were correlations between proinflammatory and anti-inflammatory cytokines. Further studies of polymorphism-related cytokines secretion are warranted. 
CREATINE KINASE MYOCARDIAL BAND, MYELOPEROXIDASE, MATRIX METALLOPROTEINASE-9 DAN TROPONIN T PADA IMA IA Hutagalung; I Patellongi; R Pakasi; M Arif; AA Mappahya; Hardjoeno .
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 17, No 3 (2011)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v17i3.1055

Abstract

In many patients presenting with chest pain symptoms at the emergency room, cardiac markers are measured not only to detect acutemyocardial infarction (AMI) but to exclude them too. Presently, the only biochemical markers used for diagnosis of MI are creatine kinasemyocardial band (CKMB) and troponin T (cTnT) as the markers of myocardial necrosis. Other biochemical markers have been sought tofind marker which can reflect the important, upstream processes in the pathophysiology of MI that therefore might give an earlier signalof ongoing MI. These markers are myeloperoxidase (MPO) and matrix metalloproteinase-9 (MMP-9) as markers of plaque destabilization.The aim of this study was to analyze the CKMB, MPO, MMP-9 activityies and troponin T level in MI. A cross sectional study had beendone at the Cardiovascular Centre Unit and Internal Ward of Dr. Wahidin Sudirohusodo Hospital during April up to August 2010 period.The laboratory tests were done at the Clinical Pathology Laboratory of Dr. Wahidin Sudirohusodo Hospital, Makassar and the Researchand Esoteric Unit of Prodia Clinical Laboratory, Jakarta. Ffifty-three patients with AMI had been examined. The results showed that themeans of CKMB, cTnT, MPO and MMP-9 were 52.4 U/L, 2.0 ng/mL, 217.9 ng/mL and 920.3 ng/mL, respectively. MMP-9 had positivelyvalue (69.8%) higher than cTnT (60.4%), MPO (58.5%) and CKMB (45.3%). Troponin T and MMP-9 were higher in patients with highCKMB. The higher level of CKMB will be the higher MPO, MMP-9 and cTnT. There were strong correlation between MPO and MMP-9, alsobetween CKMB and Troponin T. Troponin T and CKMB were lower in inferior wall infarction compared with anterolateral/anteroseptal wallinfarction. The most sensitive biochemical marker in patients with AMI is the MMP-9. The MMP-9 usage is suggested to assist the diagnosisof AMI. It is suggested a further study to establish the specificity of MMP-9 in all patients suffering with chest pain.
ASOSIASI HLA-DRB1* DAN HLA-DQB1* DENGAN IGM-RF SERUM PADA ARTRITIS REUMATOID Joewono Soeroso; Ferdinandus Maria Judajana; Handono Kalim
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 18, No 3 (2012)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v18i3.373

Abstract

Rheumatoid arthritis (RA) is a chronic progressive polygenic autoimmune disease with a high socio-economic burden. Genetic polymorphisms expressed as a shared epitope (SE) at hypervariable regions of HLA-DRB1*01/*04/*10/*14 and linkage disequilibriums of HLA-DRB1*-HLA-DQB1* with some microorganisms, increase the risk of RA. Shared epitopes are also related with the increase of serum IgM-RF as well. A cross-sectional study on 48 RA patients diagnosed by ACR Criteria 1987 from 6 hospitals in Java and Bali was done to determine the associations of HLA-DRB1* and HLA-DQB1* alleles with serum IgM-RF levels. The presence of HLA-DRB1* and HLA-DQB1* alleles were assessed through PCR-SSO. Serum IgM-RF levels (IU/mL) were assessed through ELISA. Man-Whitney U tests were used to measure the associations. Value of p<0.05 is considered to be statistically significant. The results showed that mean ranks IgM-RF levels of subjects who are bearing HLA-DRB1*04 compared to subjects who are not bearing HLA-DRB1*04 were 34.10 IU/ml (n=10) vs 21.97 IU/ml (n=38), p=0.014. While, the mean ranks of IgM-RF levels of subjects who are bearing haplotype combination of HLA-DRB1*04-HLA-DQB1*03 vs subjects who are not bearing haplotype combination of HLA-DRB1*04- HLA-DQB1*03 were 32.89 IU/mL (n=9) vs 22.56 IU/mL (n=39) (p=0.046). It is concluded that there are association of HLA-DRB1*04, and haplotype combination of HLA-DRB1*04-HLA-DQB1*03 with increased levels of IgM-RF among Indonesians with RA.
RAGAM BERBAGAI PERBENIHAN BAKTERI TERKAIT KERENTANANNYA TERHADAP ANEKA JENIS ANTIBIOTIKA Carolina M Viany S; Aryati Aryati
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 16, No 2 (2010)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v16i2.960

Abstract

Infection is the major public health problem in Indonesia which could increase its morbidity and mortality. The antibiotics treatmentwhich were given irrationally leads to bacteria susceptibility and worsen the problem. One of the efforts to manage the bacteriasusceptibility the physician has to know the bacterial pattern and its characteristic to resist various antibiotics. The information maycontribute as a reference to give antibiotic therapy in a rational manner. To know the bacterial susceptibility pattern against variousantibiotics a study was carried out using specimens derived from several hospitals which referred them to a private laboratory inSurabaya. The specimens consisted of blood, urine and sputum were referred during September 2007 up to July 2008. The identificationof the bacteria and it‘s susceptibility pattern were carried out by a conventional method and an auto analyzer (Vitek 2 Compact). Theantibiotic susceptibility test was carried out by conventional method using Kirby Bauer modifiied diffusion technique and Vitec 2 Compactusing MIC reference. The results showed that the most common bacteria found from blood was Escherichia coli which was still sensitiveto amoxicillin-clavulanic acid, amikacin, and cefepim. And from the urine was Escherichia coli which were still sensitive to meropenemfollowed by amikacin and gentamycin. Whereas from the sputum was found Streptococcus α haemolyticus which was still sensitive toamoxicillin-clavulanic acid, piperacillin tazobactam, linezolid. The result of the antibiotic susceptibility test is mostly dominated bythe Betalactam group, such as amoxicillin-clavulanic acid and carbapenem group like meropenem. Besides of that, in this study wasalso found multiple drug resistance organisms (MDRO), such as Escherichia coli ESBL, Enterobacter liquefaciens ESBL, Enterobacteragglomerans ESBL, Klebsiella ozaenae ESBL, and Klebsiella pneumoniae ESBL. The susceptibility pattern of the bacteria derived fromblood and sputum is dominated by gram positive cocci. Whereas from urine is dominated by Gram negative rods.
PROFIL ANALISIS BATU SALURAN KEMIH DI LABORATORIUM PATOLOGI KLINIK G. Ratu; A. Badji
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 12, No 3 (2006)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v12i3.870

Abstract

Urethral stones, or urolithiasis, is a pathologic and condition which is still a frequent problematic issue in the clinic, especially for itsrecurrence. This condition appears mostly in productive-age groups leading to affect their productivity. By stone analysis, the type andthe composition of concretes could be identified, so that the handling of patients could be arranged and its recurrence could be prevented.The study was carried to find out the distribution of urethral stone composition according to age and sex groups. A cross-sectional studyby means of medical records of 199 patients with urethral stone admitted to Dr. Wahidin Sudirohusodo Hospital was carried out duringMay 2002 to May 2004. Analysis was conducted microscopically and macroscopically by colorimetric and titrimetry. Urethral stoneswere found more in men 79.9% (159 of 199) than in women 20.1% (40 of 199). The peak prevalence of age between 31 to 45 yearswere 71.35%, the eldest being 86 years while the youngest was 2 years. The mostly found composition of calcium-oxalate was 87.4%,brushitt was 45.2%, and uric acid was 32.2%. The prevalence of urethral stones was still high, mostly found in 31 to 45 years of agegroup, affecting more men than women, and calcium oxalate showed the highest frequency.

Page 38 of 133 | Total Record : 1328

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