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INDONESIA
Indonesian Journal of Clinical Pathology and Medical Laboratory (IJCPML)
Published by Perhimpunan Dokter Spesialis Patologi Klinik Indonesia
ISSN : 08544263     EISSN : 24774685     DOI : https://dx.doi.org/10.24293
Core Subject : Health, Science,
Biochemistry, Genetics & Molecular Biology Health Professions Medicine & Pharmacology Neuroscience
Indonesian Journal of Clinical Pathology and Medical Laboratory (IJCPML) is a journal published by “Association of Clinical Pathologist” professional association. This journal displays articles in the Clinical Pathology and Medical Laboratory scope. Clinical Pathology has a couple of subdivisions, namely: Clinical Chemistry, Hematology, Immunology and Serology, Microbiology and Infectious Disease, Hepatology, Cardiovascular, Endocrinology, Blood Transfusion, Nephrology, and Molecular Biology. Scientific articles of these topics, mainly emphasize on the laboratory examinations, pathophysiology, and pathogenesis in a disease.
Arjuna Subject : Kedokteran - Kedokteran (Lain-Lain)
Articles 1,328 Documents
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DIAGNOSIS JANGKITAN (INFEKSI) VIRUS DENGUE DENGAN UJI CEPAT (RAPID TEST) IgA ANTI-DENGUE Sri Kartika Sari; Aryati Aryati
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 17, No 2 (2011)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v17i2.1020

Abstract

Dengue IgM, IgG Capture ELISAs and NS1 Ag ELISA become the most widely used serological methods for dengue diagnosis untilnow. Previous studies reported a possible use of IgA antibodies for dengue virus as a new serologic marker to make dengue infectionactive. In the present study, the performance of IgA anti-dengue rapid test as a new marker of dengue infection was assessed. In thisstudy, the sera were obtained from 30 dengue virus infection patients and 30 non dengue virus infection patients. Thirty dengue pairedsera were collected twice, at the time of hospital admission (acute) and at discharge (convalescent). All sera samples were characterizedusing dengue reference ELISAs (NS1 Ag, Dengue IgM and IgG capture ELISAs). The results of IgA anti-dengue rapid test were comparedwith the corresponding dengue reference tests. The sensitivity and specificity of IgA anti-dengue rapid test respectively were 78.3% (95%CI: 65.5–87.5%), and 73.3% (95% CI: 55,6–85,8%). Meanwhile, from acute sera, sensitivity of IgA anti-dengue rapid test was 83.3%(95% CI: 64.5–93.7), higher than IgM (73.3%, 95% CI: 53.8–87.0), IgG (66.7%, 95% CI: 47.1–82.1) and NS1 Ag ELISAs (60%,95% CI: 40.7–76.8). Positive IgA anti-dengue rapid test results in acute sera was higher in the secondary (91%) than primary infection(57%). IgA anti-dengue rapid test can be considered as a new marker for dengue infection, because it gives a high sensitivity, especiallyin the acute phase and in the secondary infections as well.
The Relation of 25-Hydroxyvitamin D Level with Metabolic Syndrome in Type 2 Diabetes Mellitus Patients M.I. Diah Pramudianti; Dian Ariningrum; Medityas Winda Krissinta
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 26, No 2 (2020)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v26i2.1469

Abstract

Type 2 Diabetes Mellitus (DM) is a metabolic disorder characterized by hyperglycemia. Metabolic Syndrome (MS) is acomplex metabolic disorder like hyperglycemia, obesity, dyslipidemia, and hypertension. Vitamin D controls genesassociated with the regulation of insulin and renin production. The aim of this study was to analyze the relation betweentotal levels of 25-hydroxyvitamin D [25(OH)D] and the incidence of MS in type 2 DM patients. This was an observationalstudy with a cross-sectional design conducted from October to November 2018 in Dr. Moewardi Hospital Surakarta on 84people with type 2 DM. All subjects were 34-75 years old. The research data were analyzed with a 2x2 test table to determinethe Prevalence Ratio (PR) of each study variable, then multivariate analysis with logistic regression was continued. The meantotal level of 25(OH)D was 18.01±6.10 ng/dL. Bivariate and continued with multivariate PR analysis showed poor glycemiccontrol with the incidence of MS (PR: 11.154; 95% Cl: 3.933-31.631; p=0.001); female sex (PR : 1.788; 95% Cl: 0.750-4.261;p=0.188); age < 50 year (PR: 1.644; 95% Cl: 0.614-4.404; p=0.321); and total 25(OH)D deficiency (PR: 1.250; 95%Cl: 0.317-2.022; p=0.637). Total 25(OH)D level was not associated with the incidence of MS in the type 2 DM patients. Furtherstudy was needed using healthy group control to explain the role of vitamin D in type 2 DM.
LEUKOSITOSIS BER-FLAGGING BINTANG () BERPOTENSI ADANYA INTERFERENSI ALAT ANALISIS HEMATOLOGI OTOMATIS Christine Sugiarto; Leni Lismayanti; Nadjwa Zamalek Dalimoenthe
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 17, No 2 (2011)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v17i2.1026

Abstract

Leukocytosis is a condition in which there is an increasing number of white blood cell count in the peripheral blood compared to thenormal range based on age. Several conditions can amplify leukocyte count from haematological auto analyzers, not only those whichcorrespond to the pathologic and physiologic condition, but also with other factors, such as diluent and haematological auto analyzer’smethods. The information about these factors should be evaluated to lessen errors in the patient’s diagnosis and therapy. This casereport describes a leukocytosis in a 35-day old baby boy, diagnosed as duodenal obstruction, admitted in Paediatric Surgery Department,Hasan Sadikin Hospital, Bandung. Discrepancies occurred in this patient’s leukocyte count with some different haematological autoanalyzers. The leukocyte count from the auto analyzer by impedance method and ammonium salt diluent was 129.200/mm3 which wasindicated by a star-flagged (), while from the auto analyzer with light scatter method and anhydrous sodium sulphate and sodiumchloride diluent was 9.200/mm3, from manual count by the counting chamber with Turk diluent was 14.200/mm3 and the estimationby peripheral blood smear was 7.000–10.000/mm3. False leukocytosis by auto analyzer with impedance method was caused by thelimitation of the analyzer’s method and by the erythrocyte lysine reagent (diluent) using ammonium salt. As investigated in this case,the interferences were thought as being caused by the Lyses-resistant Red Blood Cells, thus the non-lysed/lyses cells which were enlargedin size were identified as leukocytes other than erythrocytes. It can be that the white blood concluded cell count examination which isindicated by star-flagged (), or white blood cell count >100.000/mm3 must be confirmed by manual examination (counting chamberand peripheral blood smear) or by another haematological auto analyzer method that has a different and more potent diluent
PENGELOLAAN LABORATORIUM UNIT GAWAT DARURAT J. Nugraha
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 16, No 2 (2010)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v16i2.981

Abstract

The need for comprehensive and independent services in the emergency unit led to a need for a special laboratory organization inthe unit. The simple laboratory organization has a special character that has a rapid and an accurate result, but it only provides certainparameters, which related to emergency condition. In the emergency unit laboratory tests are preferred to support the procedure ofdiagnosis and clinical decisions rather than to ensure that definitive diagnosis can be continued later, when the patient was moved tothe ward. The test is chosen to support the diagnosis of diseases which requiring immediate treatment, such as myocardial infarction,stroke, emergency surgery preparation, the diagnosis of infection and electrolyte tests and blood gas analysis especially for critically illpatients. Back up instruments should be provided that can be operated anytime when there is congestion, an ideally means that willnot interrupted the services. The laboratory needs an emergency unit that can be served by means of a simple, fast, accurate thoroughtest, and it is cheap as well. The unit should also linked to LIS and HIS. So that the results can be known or accepted in all parts of thehospital. The advantage of a special laboratory emergency unit in this case may accelerate the time of service as well as saving energyand the communications will be more simple, compared to the central laboratory, and should be operated for 24 hours.
DIAGNOSTIC VALUE OF CA-125 IN PATIENTS WITH EPITHELIAL OVARIAN CANCER AT THE DR. SOETOMO GENERAL HOSPITAL SURABAYA IN 2016 Kintan Putri; Betty Agustina Tambunan; Willy Sandhika
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 25, No 2 (2019)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v25i2.1380

Abstract

Ovarian cancer is the fourth cancer with most incidence in Indonesian female with 10.238 cases in 20141. Tumor marker CA-125 is assosciated with ovarian cancer, importantly epithelial ovarian cancer. This study aims to find out diagnostic value (sensitivity, specificity, positive predictive value, negative predictive value) of CA-125 among patients with epithelial ovarian cancer in Dr. Soetomo General Hospital Surabaya in 2016. This study used analytic cross sectional method and was performed by evaluating medical records of patients suspected for ovarian malignancy in Dr. Soetomo General Hospital Surabaya in 2016. There were total 97 patients found fit for criteria of inclusion in this study. Tissue histopathological examination confirmed 66 patients have epithelial ovarian malignancy and 31 patients do not. Samples distributed using 35 U/ml as CA-125 upper limit, TP: 54.64%, FP: 19.59%, FN: 13.40%, dan TN: 12.37%. Diagnostic value obtained as follows: sensitivity 80.30%, spesificity 38.71%, positive predictive value 73.61%, negative predictive value 48%, and accuracy 67.01%. Tumor marker associated with ovarian cancer CA-125 has found high in sensitivity but low in specificity among patients with epithelial ovarian cancer in Dr. Soetomo General Hospital Surabaya in 2016.
ANALISIS CAIRAN DARAH (TRANSUDAT) DAN SERUM CAMPURAN (EKSUDAT) DI PENDERITA DENGAN REMBESAN SELAPUT PARU (EFUSI PLEURA) Didi Irwadi; Sulina Y. Wibawa; Hardjoeno Hardjoeno
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 15, No 2 (2009)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v15i2.947

Abstract

Pleural effusion is a fluid excess into pleural cavity due to transudation or exudation processes. The fluid deposited in the cavity canthreat the patient’s life. The pleural effusion could be produced in a patient with tuberculosis, cancer, cardiac failure, renal failure orviral/bacterial infection. The study is aimed to analyze the patterns of substance in the pleural effusion fluids produced by differentdiseases. A cross sectional study was performed from June 2006 to June 2007 at Clinical Pathology Laboratory of Dr. WahidinSudirohusodo Hospital, Makassar. The fluids were tested for glucose, total protein, LDH, and leukocyte count. Of 87 pleural effusion fluidsamples from 14–80 years old patients, 34.5% were transudates and 65.5% were exudates. Glucose value was higher (one tail T test,p < 0.01) in transudates group, whereas protein, LDH and leukocyte count were higher (one tail T test, p < 0.01) in exudates group.There were no significant differences of glucose, protein, LDH and leukocyte count among diseases within transudates group, as well aswithin exudates group. Staphylococcus spp., Klebsiella spp., and Acinetobacter spp., were the predominant bacteria revealed from thefluid cultures. Values of glucose, protein, LDH and leukocyte count have a different pattern between transudates and exudates groups.However, no special patterns were found among diseases within groups.
Comparison of Lipid Fractions of Icteric Sample by Using Three Devices Ummul Khair; Asvin Nurulita; Darwati Muhadi
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 26, No 1 (2019)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v26i1.1412

Abstract

COMPARISON OF LIPID FRACTIONS OF ICTERIC SAMPLE BY USING THREE DEVICES Ummul Khair1, Asvin Nurulita2,3, Darwati Muhadi2,31                      Specialist Doctoral Education Program of Clinical Pathology Science, Faculty of Medicine, Hasanuddin University/RSUP Dr. Wahidin Sudirohusodo, Makassar 2                      Department of Clinical Pathology Science, Faculty of Medicine, Hasanuddin University/RSUP Dr. Wahidin Sudirohusodo, Makassar3RSUP Dr. Wahidin Sudirohusodo, Makassar                                                                                ABSTRACTIntroductionLipid fraction assessment in laboratory includes cholesterol, HDL, LDL, Triglycerides. The icteric sample is characterized by elevated levels of bilirubin and darkness yellow sample color. This research was to determine the comparison of lipid fraction of icteric sample by using three devices.MethodCross-sectional study at Clinical Pathology Laboratory of Dr. Wahidin Sudirohusodo of Makassar. Comparison of lipid fraction of icteric samples against 3 (three) devices (Pentra, Biomajesty, Conelab) in period April - June 2018. Statistical analysis using SPSS program.Result and Discussions Total sample of 50 indicated that cholesterol level by Biomajesty was lower than Pentra400 (p < 0.001), while the cholesterol level by Conelab was significantly lower than Pentra400 (p > 0.001), but not significantly different with Biomajesty (p < 0.05). HDL level by Biomajesty did not differ significantly with Pentra400 (p > 0.05). HDL level by Conelab were significantly lower than Pentra400 (p < 0.001) and Biomajesty (p < 0.01). HDL level by Biomajesty was smallest than Pentra and Conelab. LDL level by Biomajesty did not differ significantly with Pentra400 (p > 0.05) while LDL level by Conelab was significantly lower than Pentra400 (p < 0.001) and Biomajesty (p < 0.001). It can be seen that the range of TG by Biomajesty is smallest (more accurate) than Pentra400 and Conelab. TG level by Biomajesty was significantly lower than Pentra400 (p < 0.01) while TG level by Conelab was not significantly different than Pentra400 (p > 0.05), but it was significantly higher than Biomajesty (p < 0.001).Conclusion and RecommendationThe research shows that Biomajesty is an accurate device for the measurements of HDL, LDL, and TG of icteric samples, whereas Conelab is an accurate device for cholesterol measurement of icteric samples.KeywordsLipid fraction, icteric sample, Pentra, Biomajesty, Conelab
D-DIMER IN HEMODIALYSIS PATIENTS RECEIVING CONTINUOUS AND INTERMITTENT HEPARIN Derry Heppy Fritiwi; Harun Rasyid Lubis; Adi Koesoema Aman; Herman Hariman
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 25, No 3 (2019)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v25i3.1461

Abstract

Haemodialysis is the most widely used kidney replacement therapy in Indonesia and in the world, but the procedure may trigger development on thrombogenesis. Doe to this reason, anticoagulant heparin is given during haemodialysis to prevent the development of thrombus. However, haemostasis monitoring is essential to predict the possibility of heparin induced bleeding. The use of heparin in general needs a machine to regulate continuous heparin administration, nonetheless not all hospitals have that instruments and for this reasons some centre use intermittent heparin injection. The aim of this study is to investigate whether  intermittent heparin is as effective as continuous heparin to prevent thrombus formation as well as to prevent bleeding and predict the survival outcome. Patient divided in to two grup from intermittent heparin and continuous heparin in total 50 patient were participated. Platelet count, PT, APTT, TT, fibrinogen, and D-dimer were investigated. The result demonstrates that platelet count, PT, APTT, TT, fibrinogen, and D-dimer were not significantly differed between the groups receiving intermittent and continuous heparin (p >0.05). When the test is compared between intermittent and continuous heparin in pre and post haemodialysis it is clear that there is significant increases in APTT and fibrinogen both in the intermittent and continuous heparin, but D-dimer is increased in continuous heparin only during post haemodialysis. There is no difference in the 1-year survival outcome between intermittent and continuous heparin. In conclusion, intermittent heparin produces less D-dimer increase compared to continuous heparin but it is as effective as continuous heparin. Intermittent heparin may be used as the alternative choice when continuous syringe driver machine is not available.
CORRELATION OF ATHEROGENIC INDEX OF PLASMA WITH STENOSIS LEVEL OF CORONARY ARTERY IN ACUTE CORONARY SYNDROME Ilhamifithri Ilhamifithri; Rismawati Yaswir; Eugeny Alia; Efrida Efrida
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 25, No 1 (2018)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v25i1.1491

Abstract

Atherogenic dyslipidemia is the main risk factor of Acute Coronary Syndrome (ACS), caused atherosclerosis plaque and stenosis of artery coronary. Lipid profile ratio used as a marker of cardiovascular disease severity. Atherogenic Index of Plasma (AIP) calculated as logarithm triglycerides/high-density lipoprotein cholesterol (TG/HDL-C) is a reflection of plasma atherogenicity degree and indicator of small dense low-density lipoprotein (LDL). Small dense LDL is the best predictor for cardiovascular disease, but expensive and difficult to examined. The aim of this study to determine the correlation of AIP with stenosis level of the coronary artery in ACS.  This research was an analytical study with cross-sectional design in 24 ACS patients meet the inclusion and exclusion criteria and conduct blood tests at the Central Laboratory Installation of Dr. M. Djamil Hospital Padang in January 2017- September 2017. Triglycerides and HDL-C performed by an enzymatic colorimetric method and stenosis level determined by coronary angiography. Spearman correlation was used to analyze correlation of atherogenic plasma index with stenosis level of the coronary artery, significant if p<0.05.  The subjects of this study were 20 males (83.3%) and 4 females (16.6%) with mean age 57.6(7.9) years. The mean level of HDL cholesterol and triglycerides in ACS were 34.8 (8.7) mg/dL and 155.8 (51.8)  mg/dL. The mean level of  AIP in ACS was 0.28 (0.18). Median of stenosis level of coronary artery was 80% with range 50% - 95%. Spearman correlation test showed a moderate positive correlation between AIP with stenosis level of the coronary artery in ACS (r= 0.426 ; p<0.05).  There is a moderate positive correlation between AIP with stenosis level of the coronary artery in ACS.
Platelet Count and Platelet Index as Prognosis Markers in Adult Septic Patients Steven Tiro; Raehana Samad; Uleng Bahrun
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 26, No 1 (2019)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v26i1.1357

Abstract

Sepsis is a major health problem with recent increase of incidence. One of life-threatening complications of sepsis isDisseminated Intravascular Coagulation (DIC). DIC in sepsis can trigger an increase of platelet destruction which can beassessed by platelet count and platelet index. Hence, this research aimed to analyze the correlation of platelet count andplatelet index to the prognosis of adult septic patients. This research was then performed with a retrospective longitudinalstudy design. This research used the medical record data of adult septic patients at the Dr. Wahidin Sudirohusodo GeneralHospital in Makassar. The data of platelet count and platelet index were collected from routine blood of the patients for thefirst time they were diagnosed with sepsis. In total, this research was performed on 100 adult sepstic patients. Fifty of thosepatients had a good prognosis (cured), while the other fifty patients had a poor prognosis (died). Statistical results showedthat the platelet count in the adult sepsis patients with a poor prognosis was significantly lower than those in patients with agood prognosis with median/minimum-maximum of 157,000/12,000-626,000 and 329,000/96,000-801,000, respectively(p=0.00). It was also known that there was no significant difference of MPV values between patients with poor prognosis andpatients with good prognosis with Mean±SD of 9.54±1.44 and 10.08±2.09, respectively (p=0.138). Unlike MPV values, PDWvalues in patients with poor prognosis were significantly higher than those in patients with good prognosis with Mean±SDof 16.7±6.26 and 11.25±2.13, respectively (p=0.00). Thus, it can be concluded that there was an inverse correlation betweenplatelet count and PDW value (r = -0.58). PDW value, as a result, could be used as a prognosis marker for adult septicpatients. However, it was recommended to perform further research as a prospective study by removing the possibility ofbias.

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