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Jurnal Pembelajaran dan Biologi Nukleus
Published by Universitas Labuhanbatu
ISSN : 24429481     EISSN : 26857332     DOI : https://doi.org/10.36987/jpbn
Core Subject : Science, Education, Agriculture, Social,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Environmental Science Immunology & microbiology Languange, Linguistic, Communication & Media
Jurnal Pendidikan Biologi merupakan jurnal elektronik yang merupakan wadah penerbitan artikel penelitian original yang terkait dengan penelitian pendidikan biologi. Jurnal ini dikelola oleh Program Studi Pendidikan Biologidibawah naungan LPPM Universitas Labuhanbatu. Jurnal ini diterbitkan dua kali dalam setahun pada bulan Maret dan September. Jurnal ini menyajikan hasil penelitian dibidang Pendidikan Biologi dan sains Biologi.
Arjuna Subject : Biokimia, Genetika dan Biologi Molekuler - Biokimia, Genetika dan Biologi Molekuler (Lain-Lain)
Articles 437 Documents
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Effect of Rhodopseudomonas palustris as a Bioremediation on Reducing Ammonia and Nitrite Levels of Catfish Nursery Pond Water in District Kebakkramat Regency Karanganyar Setyowati, Febriyanny Eka; Hutapea, Henny Parida
JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS Vol 9, No 2: Jurnal Pembelajaran Dan Biologi Nukleus July 2023
Publisher : Fakultas Keguruan dan Ilmu Pendidikan Universitas Labuhanbatu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/jpbn.v9i2.4331

Abstract

Catfish nursery pond water generally contains ammonia and nitrites. Ammonia and nitrite in this fish farming system start with uneaten catfish feed residue, feces, and metabolic products that enter the waters. Utilization of catfish feed which contains high protein can absorbed by fish as much as 20-30% of nutrients. The rest will be excreted in the form of feces. Feces and food scraps that settle in this water contain ammonia and nitrites, which affect the quality of the water. Efforts to maintain water quality are by reducing and disposing of pond water in the river. This is categorized as water pollution. Efforts to control this water pollution are the technique of bioremediation. The aim of bioremediation techniques is to restore the ecological balance in waters by utilizing microorganisms so that a process of degradation of harmful and complex compounds occurs into harmless compounds. The microorganisms used in this study were bacteria Rhodopseudomonas palustris which can to oxidize and decompose organic matter effectively reducing water quality. This research was conducted to examine the effect of bacterial efficiency Rhodopseudomonas palustris as a bioremediator for catfish pond water waste based on reducing levels of ammonia and nitrite. Testing the ammonia and nitrite levels using a spectrophotometric method with different wavelengths. Observations in this test were carried out for three days under anaerobic conditions and light at a temperature of 33°C. Observations showed that bacteria Rhodopseudomonas palustris had an effect on reducing nitrite levels 12.65% greater than the control. While the addition of bacteria to the observation of ammonia levels did not have a decreasing effect as indicated by the value F <F crit.
Development of Android Application-Based Learning Media Using Smart Apps Creator (SAC) on Cell Division Materials Rinaldi, Rinaldi; Hasan, Ani M.; Ibrahim, Mustamin
JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS Vol 9, No 2: Jurnal Pembelajaran Dan Biologi Nukleus July 2023
Publisher : Universitas Labuhanbatu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/jpbn.v9i2.3696

Abstract

Mobile phones are not only used as a communication tool in the millennial era, but they can also be used in the learning process. Mobile phones are rarely used as a learning tool by students. This study aims to determine the validity level of learning media based on Android applications using Smart Apps Creator (SAC) on mitotic and meiotic cell division material. This type of research is research development, or research and development (R&D), using the ADDIE model, which is modified only up to the development stage with limited trials in class XII IPA 1, SMA Negeri 1, Tapa. The results showed that the results of validating learning media based on android applications using Smart Apps Creator (SAC) on mitotic and meiotic cell division material were included in the "very valid" category with a percentage of 3.93%
Plant Effectiveness of Acorus calamus, Pistia stratiotes, Azolla pinnata as Hyperaccumulator Candidate of Phytoremediation Agent for Copper (Cu) Absorption Dewi, Juriyah Astika; Nurwahyuni, Isnaini; Munir, Erman
JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS Vol 9, No 2: Jurnal Pembelajaran Dan Biologi Nukleus July 2023
Publisher : Universitas Labuhanbatu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/jpbn.v9i2.4291

Abstract

Metals are harmful pollutants because they cannot be broken down by living organisms. An excess of metal can become toxic. The metal in question is copper (Cu). This research aimed to determine the phytoremediation effectiveness of Acorus calamus, Pistia stratiotes, and Azolla pinnata as candidates for Cu metal absorption. This investigation employed a wholly random factorial design with two factors: plant absorbent materials comprised of three plant species (Acorus calamus, Pistia stratiotes, and Azolla pinnata) and absorption concentrations of 2 and 5 ppm. The ANOVA test was used to analyze the data, followed by the average difference test. According to the results of this study, three plants—Acorus calamus, Pistia stratiotes, and Azolla pinnata—are capable of reducing Cu metal in water. The Acorus calamus plant can reduce Cu concentrations in water from 2 ppm to 96.85% and from 5 ppm to 96.80%. Pistia stratiotes plants can reduce Cu level in water from 2 ppm to 96.50% and 5 ppm to 99.94% at concentrations of 2 and 5 ppm, respectively. The Azolla pinnata plant can reduce Cu level in water from 2 ppm to 98.50% and from 5 ppm to 96.54% at concentrations of 2 ppm and 5 ppm, respectively. The highest BCF value at a concentration of 2 ppm is in the roots of Azolla pinnata plants, at 6.77 mg/kg, followed by the leaves, at 8.88 mg/kg. The maximum BCF value at a concentration of 5 ppm for Pistia stratiotes plants is 2.26 mg/kg for the roots and 2.46 mg/kg for the foliage. The greatest concentration of TF at 2 ppm in Azolla pinnata is 1.31 mg/kg, while the maximum concentration at 5 ppm in Acorus calamus is 1.98 mg/kg
Abundance and Diversity of Polychaete Worms in Kapuas Estuary, West Kalimantan Junardi, Junardi; Riyandi, Riyandi
JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS Vol 9, No 2: Jurnal Pembelajaran Dan Biologi Nukleus July 2023
Publisher : Universitas Labuhanbatu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/jpbn.v9i2.4003

Abstract

Estuaries provide valuable ecosystem services, but they are also subject to environmental pressures due to high anthropogenic activities. The diversity and abundance of Polychaete can be used as indicators of the health of the Kapuas estuary, but supporting data are currently lacking. The objective of this research is to assess the quality of the Kapuas estuary through a Polychaete community approach. The study employed a survey method by selecting ten Polychaete sampling points based on their surrounding environmental conditions. Samples were collected from each point using a Petite Ponar grab, filtered, sorted, and grouped by family. The Polychaete specimens were identified up to the genus level. The data were analyzed descriptively. A total of 108 Polychaete individuals were found, comprising nine genera and seven families. The highest abundance was observed in the genera Capitella (Capitellidae) and Sternaspis (Sternaspidae), with 778 ind.m-2and 667 ind.m-2, respectively. The diversity of Polychaete in the Kapuas estuary was classified as low, with diversity index ranging from 0.5 to 1.21. The high abundance, low diversity, and presence of indicator genera of organic pollutants such as Capitella indicate that the Kapuas estuary is affected by organic contamination
Development of Student Worksheets Based on Science Process Skills on Human Excretion System Materials Lisa, Erma; Suryani, Ira; Tambunan, Efrida Pima Sari
JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS Vol 9, No 2: Jurnal Pembelajaran Dan Biologi Nukleus July 2023
Publisher : Universitas Labuhanbatu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/jpbn.v9i2.4285

Abstract

Development of Student Worksheets (LKPD) based on science process skills on human excretory system material presented in the form of an application on a smartphone which contains human excretion system material, stages of practicum activities and questions adapted to indicators of science process skills, which are expected to be able to train participants' thinking abilities educate. The research aims to find out: (1) the feasibility of developing science process skills-based LKPD (Student Worksheets): (2) the practicality of developing process skills-based LKPD (Student Worksheets); and (3) The effectiveness of developing LKPD (Student Worksheets) based on science process skills on human excretory system material. The development model in this study is 4D which consists of 4 stages, namely define, design, development, and disseminate the research subject of 30 students of class XI IPA MAS 12 Perbaungan. The research instruments are validation sheets for material experts, linguists, and media experts on feasibility testing, practitioner validation sheets to determine practicality, pretest and posttest sheets for effectiveness testing. The results of this study prove that the skill-based LKPD science process developed is declared very feasible with a percentage of 89.39%, very practical with a percentage of 81.94% and very effective with a high category of learning improvement that has been tested with n-gain with a score of 0.73 and excellent student response with a percentage of 81.58%. The results of this study prove that the developed science process skills-based learner worksheets are very feasible, very practical, and very effective to use in the learning process
Bioprospecting of Lactobacillus sp. Starter Culture from Colostrum Breast Milk for Probiotic Milk Production from Soybeans Rahmah, Mufti Hatur; Masriany, Masriany; Ramlah, Ramlah; Isdaryanti, Isdaryanti
JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS Vol 9, No 2: Jurnal Pembelajaran Dan Biologi Nukleus July 2023
Publisher : Universitas Labuhanbatu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/jpbn.v9i2.4427

Abstract

Lactobacillus sp. is a bacterium that naturally exists in various environments, including in breast milk colostrum. This bacterium belongs to a group of lactic acid bacteria that produce lactic acid as the main product of sugar fermentation so that it has probiotic properties that also play a role in the fermentation process. This study aims to produce probiotic milk using Lactobacillus sp isolates from breast milk colostrum  with soybean (Glycine max (L.) Merril.) as the base material and then test the total lactic acid content, pH value, reducing sugar and the number of lactic acid bacteria. The production of soybean fermented milk is based on the analysis of total lactic acid content by acid-base titration method with 0.1 N NaOH standard solution, pH value by using universal pH meter, reducing sugar test by spectrophotometric method of Nelson-Somogyi and the number of lactic acid bacteria by using Glucose Yeast Pepton Agar (GYPA) medium added with CaCO3 then calculated by using Standard Plate Count (SPC) method with TPC value (Total Plate Number) is 2.3. 1012 colonies/ml, 2.5.1012 colonies/ml, 4.5.1014 colonies/ml, 4.1.1014 colonies/ml. From the results of the study it is known that Lactobacillus sp isolates from breast milk colostrum fluid can ferment soybean milk (Glycine max (L.) Merril.)
Morphometric Study of African Spurred Tortoise (Centrochelys sulcata) Based on Age Group Siagian, Tetty Barunawati; Prayogo, Muhammad Firman Bayu; Widigdaya, Danuja; Pungki, Pungki
JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS Vol 9, No 2: Jurnal Pembelajaran Dan Biologi Nukleus July 2023
Publisher : Universitas Labuhanbatu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/jpbn.v9i2.4344

Abstract

Centrochelys sulcata are kept and bred to prevent extinction. The sulkata tortoises were identified to determine their respective identities by measuring them morphometrically. Morphometric studies on sulkata tortoises are still minimal, so a morphometric study of the sulkata tortoises is needed. The purpose of this study was to identify and describe the morphometry of sulcata tortoises with three different age ranges kept in ex-situ conservation. The research sample used 15 sulcata turtles which were divided into 3 groups namely group A (1 year), Group B (2 years), and Group C (3 years). The morphometric parameters measured were body weight, head, carapace, plastron, forelimb, hindlimb and bridge. The results showed that the average body weight of group A, B and C turtles was 2,179 kg, 1,289 kg and 0,167 kg respectively. The average maximum carapace length in a row for groups A, B, C, namely 11.64, 23.18 and 16.72 cm. The average plastron lengths for groups A, B, C were 9.08, 6.44 and 9.24 cm respectively. The average forelimb length in groups A, B and C were 3.04, 6.28 and 7.78 cm respectively. The average hindlimb length for groups A, B, and C were 1.86, 3.5 and 4.04 cm, respectively. The average bridge width for groups A, B and C were 4.36, 8.94 and 10.1 cm respectively. Based on the results of morphometric measurements of the three groups of sulcata tortoises, it was shown that group C had a larger morphometric size than groups B and A, this was due to differences in age and growth factors. Sulcata tortoises group C is 3 years old and belongs to the juvenile group. Sulcata tortoise groups A and B aged 1 and 2 years are included in the yearly group
In Vitro Antidiabetic Activity and Bioactive Ingredients of Apis mellifera and Apis dorsata Binghami Nest Extracts Sibala, Henike; Naharia, Orbanus; Manampiring, Nonny; Mokosuli, Yermia Semuel
JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS Vol 9, No 2: Jurnal Pembelajaran Dan Biologi Nukleus July 2023
Publisher : Universitas Labuhanbatu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/jpbn.v9i2.4511

Abstract

Indonesia ranks 7th out of ten countries with diabetes mellitus, which continues to increase yearly. Honeycomb is rich in secondary metabolites formed from plant resins (propolis) which contain alkaloids, flavonoids, saponins, tannins, steroids and triterpenes. The research aimed to analyze the comparison of flavonoid content and antidiabetic activity with the enzyme α-glucosidase In Vitro nest extract of A. mellifera and A. dorsata Binghami. The samples used were nests of A. mellifera from northern Toraja, southern Sulawesi and A. dorsata Binghami from Southeast Minahasa, North Sulawesi. Using 96% ethanol, HPLC method of flavonoid testing and in vitro antidiabetic activity testing using α-glucosidase enzymes. The flavonoid content results were based on the number of peaks produced and the retention time of A. mellifera produced 26 compounds, and A. dorsata Bingham produced 21 compounds. The results of in vitro antidiabetic tests with α-glucoside enzyme inhibitors obtained IC50 values from the nest extract of A. mellifera of 3.605 mg/L and the nest extract of A. dorsata Binghami of 4.992 mg/L. The extract from A. mellifera had better antidiabetic potential than that of A. dorsata Binghami, although the difference is insignificant.
The Development of Scratch Software-Based Interactive Learning Media on Regulatory System Material Nurhalizah, Nurhalizah; Jayanti, Ummi Nur Afinni Dwi
JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS Vol 9, No 2: Jurnal Pembelajaran Dan Biologi Nukleus July 2023
Publisher : Universitas Labuhanbatu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/jpbn.v9i2.4462

Abstract

The research aims to develop interactive learning media based on software scratch on the material of the regulatory system and to know the validity, practicality, and efficacy of the media. The research and development method with the ADDIE model is used in this research. The research instruments used are needs analysis in the form of teacher interview guides and student questionnaires, validation sheets consisting of media and materials expert validation sheets, and implementation tools in the form of effectiveness sheets, namely pre-test and post-test, and practicality sheets, namely teacher and student response questionnaires. Based on the results and discussion, the media expert`s validity test result is 87.33%, and the material expert's result is 87.50%, indicating that it is very valid. Then, the practicality test results of the teacher's responses received a percentage of 97.50%, which is very practical. The practicality test of student responses on a small scale yielded a rate of 93.25%, which is very practical, while the field test yielded a rate of 84.30%, which is quite practical. In the effectiveness test, the percentage of n-gain obtained was 84%, which means it was effective. The results show that scratch-based interactive learning media have proven to be valid, practical, and effective. This learning media can be implied as one of the supporting media for teachers in biology subjects, particularly on regulatory system material that may boost students' motivation for learning and academic success.
Bioactive Contents, BSLT Toxicity and antioxidants from Apis dorsata Binghami and Apis mellifera nest extracts Tuegeh, Reinny Silvana; Sumampouw, Herry Maurits; Tanor, Meity Neltje; Mokosuli, Yermia Semuel
JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS Vol 9, No 2: Jurnal Pembelajaran Dan Biologi Nukleus July 2023
Publisher : Fakultas Keguruan dan Ilmu Pendidikan Universitas Labuhanbatu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/jpbn.v9i2.4514

Abstract

Beenest is rich in secondary metabolites because honeycombs, among others, are formed from plant resins (propolis). This study aimed to analyze differences in flavonoid content and in vitro antioxidant activity of Apis dorsata Binghami and Apis mellifera nest extracts. The samples used were A. dorsata from North Sulawesi and A. mellifera from South Sulawesi. The honeycomb was extracted using 95% ethanol solvent. Honeycomb extract was analyzed for its flavonoid content by the HPLC method, toxicity was tested by the BSLT method, and in vitro antioxidant activity was tested by the DPPH method. The results of the analysis of flavonoids showed that A. dorsata nest extract produced 21 types of compounds while A. mellifera produced 26 types of compounds. The toxicity test results showed that the A. dorsata nest extract had a better LC50 of 245,691 mg/l than the A. mellifera nest extract with an LC50 of 443,701 mg/l. The in vitro antioxidant test results of A. dorsata nest extract were more robust, namely IC 50 1.161 mg/l, compared to A. mellifera nest extract IC 50 2.404 mg/l. However, both were included in the category of powerful antioxidants. In vitro, anticancer test results on MCF-7 cells, A. mellifera nest extract was active with IC 50 100.02mg/l. Compared to A. dorsata extract, it was active with IC 50 102.217mg/l, but the two were not significantly different. Based on the analysis of flavonoid content, toxicity test, and antioxidant test, A.dorsata and A.mellifera honeycomb extracts have potential as in vitro antioxidants

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