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Jurnal Pembelajaran dan Biologi Nukleus
Published by Universitas Labuhanbatu
ISSN : 24429481     EISSN : 26857332     DOI : https://doi.org/10.36987/jpbn
Core Subject : Science, Education, Agriculture, Social,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Environmental Science Immunology & microbiology Languange, Linguistic, Communication & Media
Jurnal Pendidikan Biologi merupakan jurnal elektronik yang merupakan wadah penerbitan artikel penelitian original yang terkait dengan penelitian pendidikan biologi. Jurnal ini dikelola oleh Program Studi Pendidikan Biologidibawah naungan LPPM Universitas Labuhanbatu. Jurnal ini diterbitkan dua kali dalam setahun pada bulan Maret dan September. Jurnal ini menyajikan hasil penelitian dibidang Pendidikan Biologi dan sains Biologi.
Arjuna Subject : Biokimia, Genetika dan Biologi Molekuler - Biokimia, Genetika dan Biologi Molekuler (Lain-Lain)
Articles 437 Documents
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Analysis of Liver Histology Several Fish in the Air Kotok River in the Gold Mining Area, Lebong Regency Lestari, Dian Fita; Febrianti, Eliza; Wulansari, Shahnaz Sabrina; Putra, Apriza Hongko
JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS Vol 9, No 2: Jurnal Pembelajaran Dan Biologi Nukleus July 2023
Publisher : Fakultas Keguruan dan Ilmu Pendidikan Universitas Labuhanbatu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/jpbn.v9i2.4020

Abstract

Fish have habitats that are easily exposed to contamination from the environment such as heavy metal waste which can cause changes in the structure and function of body tissues. One type of waste that enters the river in the Lebong Regency River is gold mining processing waste that uses mercury and cyanide. Heavy metals can enter organisms and accumulation and biomagnification of mercury can occur in the bodies of aquatic biota such as fish. There has been no research related to fish histology in the Lebong gold mining area, so the aim of this research was to determine the histological condition of the liver organs of several fish in the form of Hemibagrus nemurus, Rasbora sp, Trichogaster trichopterus, and Macrognathus circumcinctus in the Air Kotok river in the gold mining area of Lebong Regency. The research was conducted using the paraffin method with hematoxylin-eosin staining. Data were analyzed descriptively qualitatively based on the histology of the fish liver. The results of the research on the liver histology, it shows damage to the fish liver structure which includes vacuolization, congestion, pyknosis, dilated sinusoids, melanomacrophages and leukocyte infiltration. The damage to the hepatocytes of this fish can be used as a bio-indicator of exposure to toxic substances in the Air Kotok river, one of which is heavy metal activity from gold mining, which can be a threat to the health and survival of the fish.Keywords: Bengkulu, gold mining waste, heavy metal, hepa
The Effect of IAA and BAP on Root Induction of Cattleya Orchids Harahap, Fauziyah; Sinuraya, Kenari Br; Syarifuddin, Syarifuddin; Suriani, Cicik; Ningsih, Ayu Putri; Edi, Syahmi; Nusyirwan, Nusyirwan
JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS Vol 9, No 2: Jurnal Pembelajaran Dan Biologi Nukleus July 2023
Publisher : Universitas Labuhanbatu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/jpbn.v9i2.4481

Abstract

Orchid is an ornamental plant commodity that has an important meaning in international trade. Until now, orchids are still the center of attention of farmers and lovers of ornamental plants, because of their bright potential as cut flowers and potted plants. The popular and widespread type of orchid is Cattleya sp. The Cattleya orchid is also called the queen of orchids because of its colorful flowers. Cattleya sp. orchids take a long time to propagate by seed, about 4 to 7 years, so other methods are needed to deal with them. Tissue culture is a method for isolating plant parts such as cells, tissues or organs, and cultivating them in an aseptic environment. The purpose of this study was to determine the optimal interaction of growth regulators indole acetic acid (IAA), benzyl amino purine (BAP), and their interactions with the root induction of Cattleya sp. Cytokinins interact with auxin in determining the direction of cell differentiation. In this research method, differences in concentrations of indole acetic acid (IAA: 0, 2, 4, 6 ppm) and concentrations of benzyl amino purine (BAP: 0, 0, 5, 1 ppm) were carried out. Parameters observed were the number of roots and root length. Data were analyzed descriptively and inferentially using ANOVA. The highest number of roots was obtained in the treatment medium containing 0.5 ppm BAP and 6 ppm IAA, which was 6.33. The highest root length was found in the B0I4 treatment (BAP 0 + IAA 4 ppm). The results of the analysis of the interaction variance between the IAA treatment and the BAP treatment were 0.005, meaning that there was a significant effect of the combination of IAA and BAP on the leaf length of Cattleya sp.
Formulation of Red Beet (Beta vulgaris. L) and Aloe Vera (Aloe vera) Gel Extracts as Anti-Aging Lestari, Mutiara Dwi; Maimunah, Siti; Prayoga, Andre
JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS Vol 9, No 2: Jurnal Pembelajaran Dan Biologi Nukleus July 2023
Publisher : Universitas Labuhanbatu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/jpbn.v9i2.4478

Abstract

Anti-Aging can prevent or slow down the effects of skin aging. This study aims to formulate extracts of beetroot (Beta vulgaris. L) and Aloe Vera (Aloe Vera) in gel preparations and test their anti-aging effectiveness. This research was conducted at the Laboratory of Pharmaceutical Chemistry, University of Sari Mutiara Indonesia. The tools used are skin analyzer and moisture checker. B. vulgaris Samples from Brastagi, Karo Regency and A.vera taken from Rantauprapat, Labuhan Batu Regency. Methods of implementation Beet extraction was extracted with 96% ethanol solvent, and A.vera extract from the juicer. The irritation test on 12 volunteers used nettle extract cream with the highest concentration of 0.5%. Anti-aging activity testing used 12 female volunteers aged 19-22 years, who were divided into 4 groups, namely 3 volunteers (A; Blank), administration of beetroot extract gel (B. vulgaris. L) and A.vera (B) : 3%), (C: 6%), and (D: 9%). 3. Parameters measured include moisture content, number of pores and wrinkles. Data distribution was analyzed using the One sample Kolmonogrov-Smirnov Test method. Then proceed with the analysis of the One-Way Anova method. The results of research on beet (B. vulgaris. L) and Aloe Vera (A.vera) gel preparations can be formulated in a gel dosage form. pH 6.9, W/O emulsion type, viscosity 2500-3500 cps, non-irritating, and stable on storage for 28 days. Beet fruit extract (B. vulgaris) and aloe vera viscous extract (A.vera) can be formulated in the form of an anti-aging gel that can provide an anti-aging effect at the best concentration, namely at a concentration of 9% by increasing skin moisture to 40.24%; smaller pores 0.53%; and reduce wrinkles by 0.33%
Anthocyanin, Antioxidant and Metabolite Content of Butterfly Pea Flower (Clitoria ternatea L.) Based on Flowering Phase Juswardi, Juswardi; Yuliana, Rina; Tanzerina, Nina; Harmida, Harmida; Aminasih, Nita
JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS Vol 9, No 2: Jurnal Pembelajaran Dan Biologi Nukleus July 2023
Publisher : Universitas Labuhanbatu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/jpbn.v9i2.4064

Abstract

The butterfly flowers (Clitoria ternatea L.) are used as natural dyes and herbal medicines because they contain metabolites, anthocyanins and antioxidants. The content of these metabolites is influenced by the phase of flower development. This study aims to determine the levels of anthocyanins, antioxidants and metabolites of butterfly pea flowers from different flowering phases. The Butterfly flower collection was taken from the village of Mulyaguna, Teluk Gelam, Ogan Komering Ilir, South Sumatra. The extraction was carried out with 70% ethanol solvent, and determination of the amount of anthocyanin content by spectrophotometry, antioxidant content by DPPH method, and metabolite compounds by GC-MS. Data on anthocyanin and antioxidant content were analyzed with averages and standard deviations, and GC-MS chromatograms were traced for compounds with reference to the PubChem, KEGG, ChEBI, PlantCyc, and Spectrabase websites, which then determined the dominant compound group. The results of the study on blooming butterfly pea flowers found that the antioxidant content was 6.58 ppm, higher than that of bud flowers, which were 2.55 ppm, and wither flowers, which were 1.74 ppm. The anthocyanin content of the blooming butterfly pea flower was 40.33 ppm, the withering flower was 4.36 ppm, and the bud flower was 3.60 ppm. The dominant metabolites were identified as fatty acids, organic acids, aromatics and flavanoids, followed by differences in antioxidant and anthocyanin content in the flowering phase of the butterfly pea flower
Length – Weight Relationship Analysis and Condition Factor Blood Clams (Anadara granosa) In Panipahan, Rokan Hilir Regency, Riau Province Asmita, Manja; Machrizal, Rusdi
JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS Vol 9, No 2: Jurnal Pembelajaran Dan Biologi Nukleus July 2023
Publisher : Universitas Labuhanbatu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/jpbn.v9i2.4386

Abstract

This research was conducted in the Rokan Hilir district with the aim of analyzing the length-weight relationship and condition factors of blood clams (Anadara granosa). The benefit of this research is to provide information about the length of weight and condition factors of A. granosa which can be used as a reference in cultivation and further research. This research was carried out for 90 days from October to December 2021, in the waters of Panipahan, Rokan Hilir Regency. This length-weight relationship was calculated using a linear allometric model (LAM), while the condition factor was calculated using the Fulton formula (K) and relative weight (Wr). The results of the analysis of the length-weight relationship of blood clams get a value of b <3 = 0.894, which is a negative allometric where the increase in length is faster than the growth in weight. With the value of relative weight (Wr) 71,325-149,883 and Fulton’s condition factor (K) 75,372-387,106. This is caused by environmental conditions that are quite good in supporting blood clams (A. granosa) . in the waters of Panipahan Kab. Rokan Hilir
The Diversity of Reptile Species in Mangrove Ecotourism of Kampung Rawa Mekar Jaya, Siak Regency Aldi Ardiansyah Putra; Defri Yoza; Sri Catur Setyawatiningsih
JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS Vol 9, No 2 (2023): Jurnal Pembelajaran Dan Biologi Nukleus July 2023
Publisher : Fakultas Keguruan dan Ilmu Pendidikan Universitas Labuhanbatu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/jpbn.v9i2.4266

Abstract

Rawa Mekar Jaya mangrove ecotourism is the last mangrove forest on the east coast of Sumatra Island. This mangrove forest is a home for reptiles. The reptilian fauna of the Mekar Jaya mangrove has the potential to become an ecotourism object. This study aims to determine the diversity, evenness, abundance, and status of reptile species in the Mekar Jaya mangrove ecotourism. Four 3 x 400 m transects were conducted in two habitat types: aquatic (10 m of bank) and terrestrial (30 m of bank). Observations were made in the morning and evening at low tide and repeated five times at three-day intervals. Direct collection of reptile data using a visual survey (VES) and passive sampling (glue trap). The researchers managed to capture 52 individuals, which were reptiles, consisting of 4 species (Draco sumatranus, Hemidactylus platyurus, Mabuya multifasciata, and Varanus savator) found directly in the field. The results of the interviews with the managers indicate that there are 2 species of crocodiles: Crocodylus porosus and Tomistoma schlegelii. Shannon-Wiener diversity index value was medium (H'=1.02). Evenness index was labile (0.57). The highest species abundance was M. multifasciata (0.65), while the lowest abundance was H. platyurus (0.12). All reptile species found were not protected. V. salvator is classified as a low-risk species (LC/Minor Concern) and is listed in Appendix II of CITES. Two crocodile species reported by the community are protected. T. schlegelii is vulnerable and listed in Appendix I of CITES. C. porosus is low-risk and listed in CITES Appendix II.
Duration Effects of Shrimp Paste Storage on ALT of Mold Colonies: Variations by Brand and Pre-Standard Treatment Syafitri, Nur Laila; Hastuti, Utami Sri; Prabaningtyas, Sitoresmi
JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS Vol 9, No 3: Jurnal Pembelajaran Dan Biologi Nukleus November 2023
Publisher : Universitas Labuhanbatu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/jpbn.v9i3.4690

Abstract

Shrimp paste is a commonly employed condiment or flavoring in culinary preparations. As a result of the nutrients present in shrimp paste, fungi and other microbial contaminants are able to proliferate and develop on the paste. This study aims as follows: 1) to determine ALT measurements of mold colonies to assess the quality of two brands of pre-treated shrimp paste; and 2) to identify contaminating mold species present in steamed and unsteamed shrimp paste prior to storage. Descriptive quantitative and qualitative research methods were employed within the Biology Department of FMIPA UM's Microbiology Laboratory. A series of three samplings were conducted on treated shrimp paste brands A and B, one week apart between each sampling. A volume of 90 ml of 0.1% peptone water was used to dissolve 10 grams of shrimp paste, resulting in a dilution of 10-1; this process was repeated until the dilution reached 10-5. 0. The surface of PDA (Potato Dextrose Agar) medium was inoculated with 1 ml of suspension from each dilution. The medium was subsequently incubated at a temperature of 25oC for 7x24 hours. The findings of this study indicate the following: 1) At the 28th day of storage, both brands of shrimp paste that underwent treatment with steamed and unsteamed shrimp paste remained of acceptable quality for human consumption. 2) In steamed and unsteamed shrimp paste, thirteen species of mold contaminants were identified: Chrysosporium corda, Cladosporium sphaerospermum, Penicillium frequentans, Penicillium chrysogenum, Rhizoctonia sp1., Aspergillus candidus, Fusarium equeseti, Colletotrichum ti, Moniliella acetobutens, and Rhizoctonia sp3
Secondary Metabolites of Trichoderma sp. as Antifungal Against Rice Seed-borne Pathogen Fungi Amalia, Anisa Rizki; Purnawati, Arika; Prasetyawati, Endang Triwahyu; Yanti, Vika
JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS Vol 9, No 3: Jurnal Pembelajaran Dan Biologi Nukleus November 2023
Publisher : Universitas Labuhanbatu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/jpbn.v9i3.4822

Abstract

The use of quality seeds is necessary to increase rice productivity. The existence of pathogenic fungi infections causes the quality of rice seeds to decrease and impacts the ability to grow plants. Trichoderma sp. is known to produce secondary metabolites that can inhibit the growth of pathogens. This study aimed to examine the ability of the secondary metabolites of Trichoderma sp. at various concentration levels and immersion time to suppress pathogenic fungi infections carrying rice seeds. The research was carried out by giving seed immersion treatment. Then identify the pathogenic fungi the seeds carry for further testing with the blotter test method. The parameters tested were infection rate, infection suppression, and seed viability. The identification results showed types of pathogenic fungi carried by rice seeds included Fusarium sp., Alternaria sp., Aspergillus sp. Penicillium sp., and Rhizopus sp. Research also shows that the secondary metabolites of Trichoderma sp. are effective in improving the quality of rice seeds. The test results showed that the secondary metabolites of Trichoderma sp. can suppress the level of pathogenic fungi infection. There was no interaction between the treatment of metabolite concentrations and immersion time on all test parameters. Seed immersion treatment with secondary metabolites in the 5-20% concentration range has effectively reduced fungi infection rates and increased seed viability. Meanwhile, various levels of soaking time did not affect reducing the level of fungi infection but instead affected the viability of the rice seeds.
Screening For Lipolytic Bacteria from Bonoloyo Cemetery, Surakarta Rini, Heni Sulistyo; Tyastuti, Erma Musbita; Siddiq, Yasir; Rahayu, Triastuti
JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS Vol 9, No 3: Jurnal Pembelajaran Dan Biologi Nukleus November 2023
Publisher : Universitas Labuhanbatu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/jpbn.v9i3.4944

Abstract

A cemetery is a burial place managed by the government. Inside the TPU, the body is decomposed by microorganisms, one of which is lipolytic bacteria, because the human body is composed of about 12.5–13.60% lipids. Research on lipolytic bacteria from TPU in Indonesia has not been found, even though it has the potential to obtain lipolytic bodies. Therefore, this research was conducted to select lipolytic bacteria from TPU and provide simple assistance. A total of 45 bacterial isolates from TPU Bonoloyo Surakarta were selected for their lipolytic activity using tributyrin agar media. Lipolytic activity was determined using the lipolytic index (LI). Bacterial isolates with lipolytic potential were identified based on colony morphology and Gram staining. The results showed that 30 isolates (67%) showed lipolytic activity, with the highest LI value of 5.43 (BLB 9) after 2x24 hours of incubation. Bacterial isolates that have lipolytic activity are white colonies with circular shapes. The results of Gram staining showed that the bacterial isolates belonged to a group of Gram-negative bacteria in the form of cocci. The conclusion put forward is that public cemeteries (TPU) have the potential to store isolates of lipolytic bacteria dominated by Gram-positive bacteria by as much as 57%
Potential And Mechanism Antagonistic of Trichoderma sp. Againts Colletotrichum gloeosporioides and C. aotearoa on Avocado Plant (Persea americana Mill) Mafluhah, Luluk Rofiatul; Hastuti, Utami Sri; Prabaningtyas, Sitoresmi
JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS Vol 9, No 3: Jurnal Pembelajaran Dan Biologi Nukleus November 2023
Publisher : Universitas Labuhanbatu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/jpbn.v9i3.4689

Abstract

The objective of this study is to ascertain the pathogenic and antagonistic fungi that induce disease in avocado plants (Persea Americana Mill), identify the antagonistic fungi with the greatest antagonism potential among a variety of antagonistic fungi, and elucidate the mechanism by which antagonistic fungi inhibit the growth of pathogenic fungi. From January to May 2023, this study was conducted in vitro at the Microbiology Laboratory, State University of Malang, employing a completely randomized design (CRD) comprising six replications and four interventions. The identification of mold was accomplished through macroscopically and microscopically descriptive analysis, followed by a comparison with the mold identification key book. The antagonism process was executed utilizing the dual culture method on PDA medium, and the electron microscope (SEM) was employed to witness the antagonism mechanism. The research findings revealed that T. harzianum spp. and T. viride were identified as antagonist fungi, whereas C. aotearoa and C. gloeospoiroides were identified as pathogenic fungi. The antagonistic fungus T. viride exhibited the greatest degree of antagonism, impeding the growth of C. gloeospoioroides by 77.8%. Observed antagonism occurs when the hyphae of the antagonistic mold ensnare, entangle, and pierce those of the pathogenic mold, causing harm to the region surrounding the pathogenic mold's hyphae and subsequently impeding its growth

Page 22 of 44 | Total Record : 437

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