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Rivo Hasper Dimenta
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Jurnal Pembelajaran dan Biologi Nukleus
Published by Universitas Labuhanbatu
ISSN : 24429481     EISSN : 26857332     DOI : https://doi.org/10.36987/jpbn
Core Subject : Science, Education, Agriculture, Social,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Environmental Science Immunology & microbiology Languange, Linguistic, Communication & Media
Jurnal Pendidikan Biologi merupakan jurnal elektronik yang merupakan wadah penerbitan artikel penelitian original yang terkait dengan penelitian pendidikan biologi. Jurnal ini dikelola oleh Program Studi Pendidikan Biologidibawah naungan LPPM Universitas Labuhanbatu. Jurnal ini diterbitkan dua kali dalam setahun pada bulan Maret dan September. Jurnal ini menyajikan hasil penelitian dibidang Pendidikan Biologi dan sains Biologi.
Arjuna Subject : Biokimia, Genetika dan Biologi Molekuler - Biokimia, Genetika dan Biologi Molekuler (Lain-Lain)
Articles 442 Documents
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Development of Biology E-Flashcard – Based Instructional Resource for Tenth – Grade Topic: Classification of Living Organism at SMA Negeri 2 Gorontalo – High School Halusa, Devliyanti; Latjompoh, Masra; Aydalina, Regina Valentine; Lamangantjo, Chairunnisah J.; Husain, Ilyas H.; Kumaji, Syam S.
JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS Vol 11, No 4: Jurnal Pembelajaran Dan Biologi Nukleus December 2025
Publisher : Universitas Labuhanbatu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/jpbn.v11i4.8356

Abstract

Background: Several students experience difficulties in mastering biological classification concepts. To address this issue, a digital E-Flashcard was developed as an instructional medium that supports technology-integrated learning, enhances motivation and comprehension, and offers flexible and practical use. This study evaluates the validity, practicality, and impact on learning outcomes of the E-Flashcard designed for Tenth-grade students at SMA Negeri 2 Gorontalo. Methodology: This study employed a Research and Development (R&D) method using the ADDIE development model, with a one-group pretest-posttest design involving 20 students from class X4 at SMA Negeri 2 Gorontalo. The instruments used included interview instruments, media and material validation instruments, and pretest and posttest instruments. Findings: The evaluation results indicated that the developed E-Flashcard met high feasibility standards, with material validation reaching 95.33% and media validation achieving 91%, both categorized as very feasible. Practicality assessment showed highly positive responses, reflected by a 98.5% score from teachers and 86% from students, both within the very practical category. Furthermore, analysis of learning improvement based on pretest and posttest scores yielded an N-gain value of 0.55, which falls into the moderate improvement level. These findings confirm that the E-Flashcard media satisfies the criteria of validity and practicality and demonstrates a meaningful improvement in student learning outcomes during limited-scale implementation. Contributions: This research advances digital biology instruction by introducing and empirically validating an ADDIE-based E-Flashcard model that enhances students’ conceptual understanding of biological classification, offering a scalable alternative to conventional printed flashcards in secondary education
Effect of BAP and 2,4-D Combination on the Callus Induction of Robusta Coffee (Coffea canephora Pierre ex A.Froehner) Leaf Explants Wijaya, Yovita Angeline; Restiani, Ratih; Prasetyaningsih, Aniek
JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS Vol 11, No 4: Jurnal Pembelajaran Dan Biologi Nukleus December 2025
Publisher : Universitas Labuhanbatu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/jpbn.v11i4.8338

Abstract

Background: Sustainable production of Robusta coffee (Coffea canephora) is constrained by the limited availability of high-quality planting materials, while information regarding the optimal auxin–cytokinin combinations for initiating callus cultures remains insufficient. Accordingly, this study was conducted to examine the effects of combined applications of benzyl amino purine (BAP) and 2,4-dichlorophenoxyacetic acid (2,4-D) on callus induction and subsequent growth derived from young leaf explants of Robusta coffee. Methodology: Explant cultures were established on Murashige and Skoog (MS) medium enriched with different concentrations of 2,4-D (1, 1.5, and 2 ppm) in combination with BAP (1.5, 2, and 2.5 ppm), arranged in a completely randomized design with three replications. Data collection was conducted throughout a 30-day incubation period and included measurements of callus initiation time (days), callus formation percentage, callus growth intensity, and callus morphological features. The resulting data were analyzed statistically using analysis of variance (ANOVA), followed by the Kruskal–Wallis test. Findings: Result describe that the treatment combining 2,4-D at 1 ppm with BAP at 1.5 ppm produced the most favorable response, as indicated by the shortest callus initiation period (7.33 DAP). In contrast, the application of 2,4-D at 1 ppm in combination with BAP at 2.5 ppm achieved a 100% callus induction rate and the highest callus growth intensity (2 on a 5-point scale), along with a compact callus exhibiting a yellowish-green coloration. Overall, these results highlight the critical influence of auxin–cytokinin equilibrium in enhancing callus induction in Robusta coffee and underscore its significance as a foundational reference for establishing an efficient in vitro propagation strategy. Contribution: In general, this study contributes to providing important technical information supporting the large-scale and sustainable production of high-quality Robusta coffee seedlings

Page 45 of 45 | Total Record : 442

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