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Jurnal Pembelajaran dan Biologi Nukleus
Published by Universitas Labuhanbatu
ISSN : 24429481     EISSN : 26857332     DOI : https://doi.org/10.36987/jpbn
Core Subject : Science, Education, Agriculture, Social,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Environmental Science Immunology & microbiology Languange, Linguistic, Communication & Media
Jurnal Pendidikan Biologi merupakan jurnal elektronik yang merupakan wadah penerbitan artikel penelitian original yang terkait dengan penelitian pendidikan biologi. Jurnal ini dikelola oleh Program Studi Pendidikan Biologidibawah naungan LPPM Universitas Labuhanbatu. Jurnal ini diterbitkan dua kali dalam setahun pada bulan Maret dan September. Jurnal ini menyajikan hasil penelitian dibidang Pendidikan Biologi dan sains Biologi.
Arjuna Subject : Biokimia, Genetika dan Biologi Molekuler - Biokimia, Genetika dan Biologi Molekuler (Lain-Lain)
Articles 457 Documents
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Potential Essential Oil Local Medicine Plant From West Aceh As Repellency Against Rice Weevils (Sitophilus oryzae) Lisa, Oviana; Lizmah, Sumeinika Fitria; Aminah, Siti; Sari, Putri Mustika
JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS Vol 12, No 1: Jurnal Pembelajaran Dan Biologi Nukleus March 2026
Publisher : Universitas Labuhanbatu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/jpbn.v12i1.8439

Abstract

Background: The Increased rice production will cause pest problems in storage warehouses. This pest attack can damage rice and reduce quality. The longer and thicker the stack of rice, the greater the number of rice weevil pests will appear and affect the quantity and quality of the rice itself. To overcome this problem, natural control can be carried out using medical plant as repellent so as not to damage the environment or the quality of stored rice. Based on the active compounds found in lime leaves, citronella, and marigold’s leaves, research is needed to determine the repellent activity of essential oils from citronella, lime, and marigold’s leaves against rice weevil (S. oryzae).  Methodology: The method used in this research is a factorial Completely Randomized Design (CRD) experimental method with two factors and three replications. The implementation of the research was consists of Sitophilus oryzae Test Pest Propagation, Medicinal Plant Sample Preparation, Essential Oil Repellent Activity Test Using Rice, and Data Analysis using Two-Ways ANOVA with Post-hoc Tukey HSD 5%. Results: The result showed that the concentration of essential oils from Marigold, lime, and citronella leaves affected the percentage of rice weevil repellent. At a concentration of 100%, the essential oil extracted from marigold leaves exhibited lower repellent activity (90%) compared to the essential oil derived from lime leaves (94%) and citronella leaves (92%). On the other hand, the duration of the observation period did not significantly affect the repellency activity of essential oils derived from lime leaves, citronella leaves, or marigold leaves. The concentration of essential oils from Marigold, lime and citronella leaves on the best percentage of rice weevil repellent was 100% with the repellency percentage above 90%. Contributions: These findings indicated that essential oils derived from these medical plants have potential as environtmentally friendly repellents for controlling rice weevil infestations in stored rice
Diversity of Sea Cucumbers (Holothuroidea) in the Waters of Sarang Island and the Nenek Strait, Batam City, Riau Islands, Indonesia Novianti, Devi; Ramses, Ramses; Ismarti, Ismarti; Amelia, Fitrah
JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS Vol 12, No 1: Jurnal Pembelajaran Dan Biologi Nukleus March 2026
Publisher : Universitas Labuhanbatu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/jpbn.v12i1.8798

Abstract

Background: Sea cucumbers (Holothuroidea) are key benthic organisms that contribute to coastal ecosystem stability through bioturbation, organic matter decomposition, and their role in marine food webs. However, despite their ecological and economic importance, information on the diversity and community structure of sea cucumbers in the waters of Batam City, remains limited. This study aimed to analyze the diversity of sea cucumbers in the waters of Sarang Island and the Nenek Strait, as a basis for evaluating ecological conditions and supporting sustainable marine resource management. Methodology: Sampling was conducted in the intertidal zone using the sweeping method during nighttime to accommodate the nocturnal activity of sea cucumbers, using two transects at each sampling station. Species identification was based on morphological and visual characteristics, while environmental parameters such as temperature, pH, salinity, dissolved oxygen, water transparency, and current velocity were measured in situ. Findings: Five sea cucumber species were identified, namely Stichopus herrmanni, Holothuria leucospilota, H.scabra, Bohadschia aegyptia, and H. fuscopunctata. Community structure was then analyzed using the Shannon–Wiener diversity index (H’), evenness index (E), and Simpson’s dominance index (C). At Sarang Island, 25 individuals representing five species were recorded, with Actinopyga mauritiana dominating 64% of the population, resulting in moderate diversity (H’ = 0.576), low evenness (E = 0.358), and high dominance (C = 0.453). In the Nenek Strait, 22 individuals from three species were identified, showing moderate diversity (H’ = 1.059), high evenness (E = 0.964), and low dominance (C = 0.360), actinopyga mauritiana dominated 64% of the population, likely due to its higher tolerance to local environmental conditions and its preference for sandy–muddy substrates commonly found in the study area, indicating a more balanced community structure. Contributions: These findings highlight spatial variation in sea cucumber communities related to habitat characteristics and ecological pressure, contributing baseline scientific data on the diversity and community structure of Holothuroidea in Batam coastal waters
Sex-Specific Bands in Dioecious Plant Jernang Rattan (Daemonorops draco) Based on RAPD Markers Nurhafitri, Amanda; Mansyurdin, Mansyurdin; Tjong, Djong Hon; Syamsuardi, Syamsuardi; Asra, Revis; Idris, Muhammad
JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS Vol 12, No 1: Jurnal Pembelajaran Dan Biologi Nukleus March 2026
Publisher : Universitas Labuhanbatu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/jpbn.v12i1.9059

Abstract

Background: Daemonorops draco (Willd.) Blume, or dragon’s blood rattan, is a dioecious species that produces high-value resin but is increasingly threatened by habitat degradation. Effective cultivation and conservation require early identification of male and female plants, which remains challenging at the seedling stage. Although RAPD markers have been used for sex identification in dioecious plants, studies on D. draco are still limited. Therefore, this study aims to screen sex-specific bands in D. draco using the RAPD approach. Methodology: Samples of six male and six female individuals collected from Jambi were analyzed, with DNA isolated using a modified CTAB protocol and amplified using 44 RAPD primers. The products were separated by 2% agarose gel electrophoresis. Bands were considered sex-specific if they consistently appeared only in one sex and were absent in the other. Findings: Out of the 44 primers tested, 39 successfully amplified D. draco DNA, generating a total of 662 bands. Among them, primer OPA-14 (455 bp) and OPC-19 (310 bp) produced male-specific bands that were consistently present in all male individuals, while primer OPB-10 (850 bp) generated a female-specific band detected in all female individuals. The male-specific 455 bp band from OPA-14 and 310 bp from OPC-19, and the female-specific 850 bp band from OPB-10 were identified. These bands are recommended for development into SCAR markers for early sex identification in D. draco. Contributions: The findings support the provision of male and female seedlings to enhance conservation and cultivation programs
Morphometric Characteristics, Length–Weight Relationship, and Condition Factor of Kapis Clams (Pecten maximus, Linnaeus, 1758) in Sarang Island Coastal Waters Siregar, Khoirotunnisa; Ramses, Ramses; Puspita, Lani; Ismarti, Ismarti
JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS Vol 12, No 1: Jurnal Pembelajaran Dan Biologi Nukleus March 2026
Publisher : Universitas Labuhanbatu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/jpbn.v12i1.8793

Abstract

Background: This study examined the morphometric characteristics, length–weight relationship, and condition factor of scallops belonging to the family Pectinidae in the waters of Sarang Island and Mecan Island, Belakang Padang District, Batam City. This research is important because scientific information regarding the biological characteristics and population condition of scallops in this area is still limited, while exploitation by local fishermen continues to occur. Methodology: The study was conducted from August to October 2024 using a quantitative descriptive approach. Sampling was carried out through diving surveys at three fishing stations using a sweeping technique along transects covering an area of approximately 100 m² at depths ranging from 2–6 m. Morphometric measurements included shell length, shell width, shell thickness, dorsal width, and total body weight. The length–weight relationship was analyzed using logarithmic regression, while physiological condition was evaluated using the relative condition factor (Kn). Findings: A total of 88 individuals were collected.. Water quality parameters showed suitable environmental conditions with temperature ranging from 29–31 °C, salinity 30–32 ppt, dissolved oxygen 5.2–6.4 mg/L, and pH 7.6–8.1. The growth exponent (b = 2.5877) indicates a negative allometric growth pattern, where shell length increases faster than body weight. This pattern may reflect ecological adaptation and environmental conditions in the study area. Contributions: These findings provide baseline biological information for sustainable scallop resource management
The Effectiveness of Adiwiyata Programme on Environmental Awareness of SMP Negeri 27 Makassar Students Suyanti, Try; Yusal, Muh Sri; Hasyim, Ahmad; Herlina, Herlina
JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS Vol 12, No 1: Jurnal Pembelajaran Dan Biologi Nukleus March 2026
Publisher : Universitas Labuhanbatu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/jpbn.v12i1.8368

Abstract

Background:  The Adiwiyata school programme aims to foster a sense of responsibility among the school community regarding environmental protection and management through good school governance. This study aims to examine the effectiveness of the Adiwiyata programme in fostering environmental awareness among students at SMP Negeri 27 Makassar as contribution towards achieving the Sustainable Development Goals. Unlike previous studies, which generally employed a qualitative descriptive approach, this study utilised a quantitative approach to obtain more measurable empirical evidence regarding the causal relationship between the programme and students’ attitudes. Methodology: The research methodology employed a quantitative approach using linear regression analysis and the coefficient of determination test. Sampling was conducted using purposive sampling. Instruments used included questionnaires, observation sheets, and documentation to measure programme implementation and students’ environmentally conscious behaviour. Findings: The study indicate that the implementation of the Adiwiyata programme and students’ environmentally conscious attitudes fall into the ‘very good’ category, carried out in a participatory manner involving the entire school community and relevant partners. The coefficient of determination value of 0.681 indicates that the Adiwiyata programme has a strong influence on students’ environmentally conscious behaviour, whilst 31.9% is influenced by other factors outside scope of this study. Contributions: This study serve as a source of literature and measurable empirical evidence regarding the impact of the Adiwiyata programme on students’ environmental awareness, integrating cognitive, affective and behavioural dimensions. In practical terms, this study can serve as a reference for other schools wishing to implement similar programmes
Soil Physical Quality Indices Across Various Land-Use Systems in Giripurno Village, Kota Batu Pembayun, Rida Yeni; Purwadi, Purwadi; Wijayanti, Fitri
JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS Vol 12, No 1: Jurnal Pembelajaran Dan Biologi Nukleus March 2026
Publisher : Universitas Labuhanbatu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/jpbn.v12i1.8484

Abstract

Background: Continuous land use and soil cultivation without proper management can affect soil productivity and physical properties. The quality of soil physical properties plays a crucial role in agricultural development. Soil physical properties are key factors supporting crop production because they influence the availability of water and plant nutrients; therefore, it is essential to understand their quality. This study aims to determine and evaluate the quality of soil physical properties on mixed-use farmland, dry fields, and paddy fields in Giripurno Village. Methodology: This study was conducted using a survey method, and soil sampling was performed using purposive sampling, resulting in 12 sample points. Soil samples were collected using a soil sampling ring at soil depths of 0–30 cm and 30–60 cm for each land-use type. The observed soil physical parameters were soil texture, bulk density, permeability, particle density, porosity, and aggregate stability. Data analysis was performed using Excel 2013, and the soil physical property quality index was calculated based on criteria specific to each land-use type. Findings: The results of the study showed that the best soil physical property quality index value was found in mixed gardens at 0.84 with a “good” rating, while the worst was found in rice fields at 0.36 with a “bad” rating. The higher the soil physical quality index, the better the soil quality of a given plot of land. Land use that involves proper, non-excessive cultivation will help maintain soil quality. Contributions: The findings provide a practical basis for sustainable soil management by identifying land-use systems with better physical soil conditions and highlighting the need to improve degraded paddy field soils
Detection of Soil Organic Matter Using IoT-Based Soil Color Sensors with Random Forest Method Andriansyah, Muhammad Afifi; Rakhmawati, Lusia; Buditjahjanto, I Gusti Putu Asto
JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS Vol 12, No 1: Jurnal Pembelajaran Dan Biologi Nukleus March 2026
Publisher : Universitas Labuhanbatu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/jpbn.v12i1.9077

Abstract

Background: Soil organic matter (SOM) is an important indicator of soil fertility that plays a role in agricultural productivity and ecosystem sustainability. However, conventional laboratory-based methods still have limitations in terms of time, cost, and do not support real-time monitoring. Therefore, an approach based on sensors and machine learning is needed for quick and efficient estimation. This study proposes an Internet of Things (IoT)-based system that integrates an RGB soil color sensor (TCS3200) and a pH sensor to estimate soil organic matter content using a Random Forest algorithm. Methodology: Laboratory analysis was conducted using the Walkley–Black method. Soil samples were taken from seven locations (T1–T7). The Random Forest model was developed with parameters n_estimators = 100 and max_depth = 10, and validated using a train-test split method (80:20). Findings: The results showed that darker-colored soils have higher organic carbon content. The model's error values indicated an MAE of 0.031 and an RMSE of 0.032. The Random Forest model achieved a classification accuracy of 85.7% and a coefficient of determination R² ≈ 0.97. Contributions: This study contributes by developing an integrated IoT and machine learning system capable of quickly, accurately, and cost-effectively estimating soil organic matter to support precision agriculture

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