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YOHANES BARE
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JL. KESEHATAN, NO 03, KELURAHAN BERU, KECAMATAN ALOK TIMUR, KABUPATEN SIKKA, PROVINSI NUSA TENGGARA TIMUR 86111
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Spizaetus: Jurnal Biologi dan Pendidikan Biologi
Published by Universitas Nusa Nipa
ISSN : 2716151X     EISSN : 2722869X     DOI : http://dx.doi.org/10.55241/spibio
Jurnal ini fokus pada bidang kajian biologi sains dan pendidikan biologi. Kami menerima artikel berupa hasil penelitian maupun kajian pustaka yang original dan belum pernah dipublikasi.
Arjuna Subject : Umum - Umum
Articles 20 Documents
Search results for , issue "Vol 6 No 2 (2025): Spizaetus: Jurnal Biologi dan Pendidikan Biologi" : 20 Documents clear
Pengaruh Perbedaan Pot Hitam dan Softpot terhadap Jumlah Anakan dan Panjang Akar Anggrek Dendrobium sonia ersakul Filany, Devany England; Dzakiy, M Anas
Spizaetus: Jurnal Biologi dan Pendidikan Biologi Vol 6 No 2 (2025): Spizaetus: Jurnal Biologi dan Pendidikan Biologi
Publisher : Program Studi Pendidikan Biologi, FKIP, Universitas Nusa Nipa

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55241/spibio.v6i2.437

Abstract

 Anggrek merupakan tanaman hias yang cantik dengan laju pertumbuhan yang panjang. Perkembangan anggrek dapat dipengaruhi secara optimal dengan pemilihan jenis pot yang tepat. Tujuan dari penelitian ini adalah untuk membandingkan pengaruh penggunaan pot hitam dan softpot pada anggrek Dendrobium sonia ersakul ditinjau dari jumlah anakan dan panjang akar baru yang muncul. Penelitian ini dilakukan di CV. Candi Orchid Semarang dari bulan Juni sampai Agustus. Penelitian ini adalah bagian dari kelompok eksperimen yang menggunakan pengacakan sempurna pada satu faktor yaitu pot hitam dan softpot. Pendekatan yang dilakukan yaitu analisis deskriptif kuantitatif. Hasil penelitian menunjukkan bahwa suhu media tanam dipengaruhi oleh warna pot yang digunakan. Hormon yang ada pada media tanam mungkin dipengaruhi oleh suhu. Berdasarkan penelitian dapat disimpulkan bahwa tidak terdapat perbedaan nyata jumlah anakan yang dihasilkan antara pot hitam dan softpot, namun terdapat perbedaan waktu tumbuh yang dikendalikan oleh hormone sitokinin. Panjang akar baru yang muncul berbeda nyata antara pot hitam dan softpot, hal ini dipengaruhi oleh hormon auksin.
Ini Jenis-Jenis Tumbuhan Mangrove Di Kawasan Ekowisata Mangrove Kolam Susuk Kabupaten Belu: Jenis-jenis Tumbuhan Mangrove Wilhelmina Djawa, Viennye; Pani, Emilianus; Semiun, Chatarina G.
Spizaetus: Jurnal Biologi dan Pendidikan Biologi Vol 6 No 2 (2025): Spizaetus: Jurnal Biologi dan Pendidikan Biologi
Publisher : Program Studi Pendidikan Biologi, FKIP, Universitas Nusa Nipa

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55241/spibio.v6i2.543

Abstract

Mangroves are a type of plant found in tropical coastal vegetation communities, which have large and broad potential benefits for the stability of a coastal ecosystem. In general, in a mangrove ecosystem area there are 2 (two) types of mangrove plants, namely true mangroves and associated mangroves. This research aims to find out about the types of mangrove plants and environmental conditions in the Susuk Pond mangrove ecotourism area, Belu Regency. The method used in this research is the Purposive Sampling method, namely determining stations by looking at the tidal zone and the mangrove growth substrate. Data collection at 3 (three) observation stations, namely Station 1 (S1), Station 2 (S2) and Station 3 (S3). The data collection method uses the roaming method. The data taken is data on mangrove plant types, abiotic parameter data and biotic parameter data. The data that has been collected is analyzed qualitatively descriptively. The results of the research show that in the Susuk Pond mangrove ecotourism area, Belu Regency, 23 (twenty-three) types of mangrove plants were found, consisting of 17 (seventeen) types of true mangroves and 6 (six) types of secondary mangroves. The types of true mengrove plants are Acanthus ebracteatus, Aegiceras floridum, Avicennia alba, Avicennia officinallis, Bruguiera cylindrica, Bruguiera gymnorrhiza, Bruguiera parviflora, Bruguiera sexangula, Ceriops tagal, Excoecaria agallocha, Laguncularia racemosa, Lumnitzera racemosa, Rhizophora apiculata, Rhizophora mangle, Rhizophora mucronata, Rhizophora stylosa, and Sonneratia alba. Meanwhile, the following types of mangrove plants are Celtis tenuifolia, Opuntia rastrera, Pandanus odorattisima, Pandanus tectorius, Pisticia vera, and Vitex trifolia. Environmental conditions, namely water temeparature ranging from 29?C-34?C, water temperature ranging from 28,5?C-32?C, sandy and mudy substrates, salinity ranging from 0,97 ?/oo-3,73 ?/oo, pH ranging from 6,95-7,23, condutivity ranging from 17,3 ?s/cm-84,4 ?s/cm and TDS ranging from 15,3 ppm-93,6 ppm. The range of environmental and water quality parameter valuases, if associated with the ideal water quality range for the growth and survival of mangrove plants of marine biota are in ideal conditions or can still be tolerated for growth and survival.
Efektivitas Antibakteri Ekstrak Etanol Kulit Umbi Ubi Jalar Ungu (Ipomoea batatas L) terhadap Staphylococcus aureus dan Salmonella typhi. Dewi Isnaeni; B, Suherman
Spizaetus: Jurnal Biologi dan Pendidikan Biologi Vol 6 No 2 (2025): Spizaetus: Jurnal Biologi dan Pendidikan Biologi
Publisher : Program Studi Pendidikan Biologi, FKIP, Universitas Nusa Nipa

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55241/spibio.v6i2.544

Abstract

Research about antibacterial effectiveness of ethanol extract of purple sweet potato tuber skin (Ipomoea batatas L.) against Staphylococcus aureus and Salmonella typhi has been conducted. This study aims to determine the antibacterial effectiveness of ethanol extract of Purple Sweet Potato Tuber Skin against the growth of Staphylococcus Aureus and Salmonella Typhi and the most effective concentration. This research is expected to provide additional information about alternative medicine that has proven effective in laboratory testing for the community as an antibacterial agent. This type of research uses the agar diffusion method, Staphylococcus Aureus, namely with an average zone of resistance for concentrations of 2% w/v, 4% w/v, 6% w/v and 8% w/V of 12 mm, 16.26 mm, 24.26 mm, 27.1 mm and for positive control 31.16 mm, negative control around 6 mm. For the growth of Salmonella typhi, namely with an average zone of inhibition for concentrations of 2% w/v, 4% w/v, 6% w/v and 8% w/V of 12.83 mm, 17 mm, 25 mm, 26, 6 mm and for positive controls 32.5 mm, negative controls around 6 mm. Statistical tests using SPSS 24 (Statistical Product and Service Solutions) based on Analysis of Variance (ANAVA) showed that there were significant differences between each treatment and the positive control. Conclusion Purple Sweet Potato Tuber Skin is effective as an antibacterial.
Analisis Kekerabatan Menggunakan Metode Fingerprint DNA pada Ikan Sepat (Ordo Anabantiformes) di Perairan Kecamatan Kualuh Leidong Ritonga, Desi; Idami, Zahratul; Manulu, Kartika
Spizaetus: Jurnal Biologi dan Pendidikan Biologi Vol 6 No 2 (2025): Spizaetus: Jurnal Biologi dan Pendidikan Biologi
Publisher : Program Studi Pendidikan Biologi, FKIP, Universitas Nusa Nipa

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55241/spibio.v6i2.550

Abstract

Gourami is a type of freshwater fish that is widely distributed in Indonesia, including in the waters of Kualuh Leidong District, North Sumatra. However, the kinship relationship between gourami species in the region has not been widely studied. Therefore, this study aims to analyze the kinship relationship of several gourami species found in the waters of Kualuh Leidong using the DNA fingerprint method. This study uses a molecular characterization method with several stages, namely collecting fish samples, isolating and extracting DNA, amplifying DNA using Polymerase Chain Reaction (PCR) with several primers, electrophoresis, and analyzing data using NTSYS software. Sampling was carried out by collecting specimens from the waters of Kualuh Leidong, including four types of gouramis, namely Trichopodus trichopterus, Trichopodus pectoralis, and Helostoma temmincki. The electrophoresis results showed that the DNA of the gourami fish could be amplified with primers OPA 02, OPC 02, and UBC 456, but not amplified with primer OPA 20. The DNA banding pattern obtained consisted of polymorphic and monomorphic bands with varying locus sizes. Jaccard coefficient analysis showed a high level of kinship (>70%), with the species Trichopodus pectoralis having a similarity of 0.78, Trichopodus trichopterus 0.76, and Helostoma temmincki 0.71. The results of this study can be used as a basis for the conservation and management of gourami fish resources in the waters of North Sumatera.
Analisis Konsentrasi CO2 di Kawasan Pasar Wisata Bojonegoro Evi Cici Kumala; Mulyanti, Heri; Purwaningrum, Solikhati Indah
Spizaetus: Jurnal Biologi dan Pendidikan Biologi Vol 6 No 2 (2025): Spizaetus: Jurnal Biologi dan Pendidikan Biologi
Publisher : Program Studi Pendidikan Biologi, FKIP, Universitas Nusa Nipa

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55241/spibio.v6i2.553

Abstract

Carbondioxide (CO?) is one of the greenhouse gases that contribute to climate change and global warming. Indoor and urban CO? concentrations can affect air quality and human health. Pasar Wisata Bojonegoro is a central economic hub with high mobility and trading activities, which may increase CO? emissions. This study aims to identify peak CO? concentration hours in the area and compare them with the air quality standards set by the WHO. A quantitative research method was used, involving direct observation and measurement over 7 (seven) days at a single observation point. The results show that on weekdays, the peak CO? concentration occurred between 16:00 and 16:59 WIB, reaching 424 ppm, while the lowest concentration was recorded between 18:00 and 18:59 WIB at 351 ppm. On weekends, the peak concentration was 441 ppm between 16:00 and 16:59 WIB, while the lowest was 113 ppm between 21:00 and 21:59 WIB. Several weekdays recorded CO? concentrations exceeding the WHO standard (310–330 ppm), with the highest reaching 466 ppm. However, CO? concentrations on Thursdays and weekends remained within the acceptable limits. The conclusion of this study shows that the results of CO? concentration on weekdays (Monday, Tuesday, Wednesday, Friday) exceed the quality standard with CO? concentrations of 466 ppm, 462 ppm, 413 ppm, and 433 ppm. Meanwhile, on weekdays (Thursdays) and weekends, it meets the quality standards with CO? concentrations of 217 ppm, 243 ppm, and 267 ppm.
Pengaruh Pemberian Rendaman Kulit Bawang Merah (Allium cepa L.) dengan Media Tanam Ampas Tebu terhadap Pertumbuhan Tanaman Pakcoy (Brassica rapa L.) Siregar, Nuri Jannah Tussadiyah; Idris, M; Selvia, Irda Nila
Spizaetus: Jurnal Biologi dan Pendidikan Biologi Vol 6 No 2 (2025): Spizaetus: Jurnal Biologi dan Pendidikan Biologi
Publisher : Program Studi Pendidikan Biologi, FKIP, Universitas Nusa Nipa

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55241/spibio.v6i2.554

Abstract

Pakcoy comes from the Brassicaceae family, known for being rich in nutrients, including vitamins A, C, K, as well as fibres and minerals. Onion skin contains bioactive compounds that have the potential to be organic fertilizers, while sugarcane bagasse is agricultural waste that can be used as an alternative planting medium. This study aims to determine the effect of soaking shallot skin using sugarcane bagasse planting media for the growth of Pakcoy (Brassica rapa L.). This study was conducted in Tuntungan II village using the factorial Randomized Block Design (RAK) method consisting of two factors and 3 replications with 3 treatment levels. The parameters observed included relative growth rate, net assimilation rate, number of leaves, total leaf area, wet weight and dry weight of pakcoy plants. The results showed that the combination of onion skin soaking gave a significant effect on all red parameters (B2: 250 ml/500 ml water) and bagasse gave a significant effect on the net assimilation rate of 2-3 MST and 4-5 MST in the treatment of 500 gr/polybag bagasse but on other parameters the control treatment was better. Thus, the provision of onion skin soaking as organic fertilizer with bagasse planting media on the growth of pakcoy plants can be applied in agriculture, because this treatment has a significant effect on all treatments.
Penurunan Total Coliform Mata Air dengan Desain Biofilter Limbah Organik Djawa, Viennye Wilhelmina; Sarifan, Gracyana Putri; Tahu, Maria Astin Rosari; Semiun, Chatarina Gradict
Spizaetus: Jurnal Biologi dan Pendidikan Biologi Vol 6 No 2 (2025): Spizaetus: Jurnal Biologi dan Pendidikan Biologi
Publisher : Program Studi Pendidikan Biologi, FKIP, Universitas Nusa Nipa

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55241/spibio.v6i2.555

Abstract

Unconserved springs tend to be polluted by various organic wastes. Accumulation of organic waste causes water to be contaminated with coliform bacteria, thus reducing water quality. Biofilter technology can be applied to reduce the number of coliform bacteria. This study aimed to determine the ability of BioFilter K4Ser-Col (moringa seeds, rice husks and kesambi charcoal) to reduce total coliform in spring water. The research location was at the Riumata Spring, Nekbaun village, West Amarasi sub-district, Kupang district. The work consisted of preparing the BioFilter K4Ser-Col media, taking water samples, and testing MPN coliform. MPN coliform testing was done twice before and after the water filtration on the BioFilter K4Ser-Col media. The study's results showed a decrease in the MPN value before and after filtering. The MPN coliform value before filtering was 1600 MPN/100 mL. After filtered water on the BioFilter K4Ser-Col media, it decreased to 23.23 MPN/100 mL. This value had met the national water quality standards for the class I category based on PP No. 22 of 2021. Thus, the use of BioFilter K4Ser-Col media could greatly reduce the number of coliform bacteria in water.
Analisis Peran Transpor Membran dalam Respons Stres Abiotik: Implikasi pada Pertumbuhan dan Perkembangan Tanaman Lestari, Dian Dwi; Febrianti, Nia; Adnan
Spizaetus: Jurnal Biologi dan Pendidikan Biologi Vol 6 No 2 (2025): Spizaetus: Jurnal Biologi dan Pendidikan Biologi
Publisher : Program Studi Pendidikan Biologi, FKIP, Universitas Nusa Nipa

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55241/spibio.v6i2.556

Abstract

Abiotic stress is an environmental condition that is unfavorable for plants such as drought, salinity, heavy metal contamination and nutrient deficiencies. Prolonged abiotic stress causes the stability of plant cell organelles to be disrupted, thereby inhibiting plant growth and development. This research aims to examine the role of membrane transport in responding to environmental stress, especially abiotic stress, and the implications for plant growth and development. The research method used was a literature study using meta-analysis techniques assisted by prism diagrams in analyzing 14 relevant scientific articles from various national and international journals. The analysis was carried out descriptively on the results of previous research. The results show membrane transport has a role in increasing plant resistance to abiotic stress. Cell membrane structure consisting of lipids, proteins and carbohydrates has been proven to increase plant safety from environmental stress. So it can be concluded that membrane transport has a role in mediating tolerance, resistance, and resolving plant stress conditions through maintaining cellular homeostasis. Implications of this research can help expand knowledge about how plants respond to unfavorable environmental conditions through membrane transport mechanisms and can be basis for developing plant varieties are resistant to various forms of environmental abiotic stress.
Pemanfaatan Limbah Sekam Padi (Oryza sativa Linn.) sebagai Media Fermentasi untuk Produksi Enzim Selulase oleh Aspergillus niger Fadhilah, Eksa Adhwa; Peristiwati; Surakusumah, Wahyu
Spizaetus: Jurnal Biologi dan Pendidikan Biologi Vol 6 No 2 (2025): Spizaetus: Jurnal Biologi dan Pendidikan Biologi
Publisher : Program Studi Pendidikan Biologi, FKIP, Universitas Nusa Nipa

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55241/spibio.v6i2.559

Abstract

This study aims to optimize the production of cellulase enzyme by the cellulolytic fungus Aspergillus niger using rice husk powder (Oryza sativa Linn.) as substrate. Cellulase enzymes have important roles in various industrial applications, including the bioconversion of lignocellulosic waste into high-value products such as bioethanol, pulp, paper, and animal feed. The utilization of rice husk as a substrate offers economic and environmental advantages due to its abundant availability, low cost, and ability to reduce the volume of agricultural waste. The research method used a quantitative experimental approach to evaluate the effect of fermentation parameters, such as pH, temperature, and incubation time, on enzyme production. A. niger isolates were developed on carboxymethylcellulose (CMC) media to ascertain their cellulolytic capacity, with measurements of biomass, pH, temperature, and enzyme activity. The results showed that the biomass of A. niger increased from hour 0 to hour 96 at pH 4.5 to 5.5, and temperature 29.5°C to 30.5°C. The optimum condition for enzyme production was found at pH 4.5 and temperature 30.5°C, with the highest enzyme activity reaching 0.736 U/mL. These findings suggest that optimization of cellulase enzyme production from rice husk not only improves enzyme production efficiency, but also contributes to more sustainable waste management.
Eksplorasi Keanekaragaman Jamur Edible di Pasar Jakarta dan Bekasi: Potensi Pangan Fungsional dan Bioaktivitasnya Angela, Ezra; Purba, Dian Pirma Natasari; Siahaan, Frisca Olivia; Situmorang, Agnes Feronika; Hutabarat, Anne Serafin; Mamangkey, Jendri; Sunarto
Spizaetus: Jurnal Biologi dan Pendidikan Biologi Vol 6 No 2 (2025): Spizaetus: Jurnal Biologi dan Pendidikan Biologi
Publisher : Program Studi Pendidikan Biologi, FKIP, Universitas Nusa Nipa

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55241/spibio.v6i2.562

Abstract

Biodiversity, especially edible mushrooms, plays a crucial role in food sustainability and public health. Edible mushrooms, such as Shiitake and oyster mushrooms, are known to be rich in nutrients and bioactive compounds that are beneficial for health, including antioxidant and anticancer effects. This research aims to identify various types of edible mushrooms sold in traditional and modern markets in several areas of Jakarta and Bekasi, as well as to evaluate their potential as sources of functional food and biopharmaceuticals. The exploration method was conducted through direct visits to several markets, where data was collected and analyzed descriptively. The results indicate that there are 10 species of edible mushrooms in the Jakarta area and 8 species in Bekasi, with ear mushrooms and oyster mushrooms being the most commonly found. Public knowledge regarding the health benefits of edible mushrooms is still limited, thus this research is expected to raise awareness about the importance of utilizing edible mushrooms as part of a healthy diet and their potential in the food and health industry. Public awareness related to a healthy diet, edible mushrooms can be used as an attractive alternative in natural medicine and nutrition, supporting food sustainability in Indonesian society.

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