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Arif Rahman Hikam
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Fakultas Biologi Universitas Jenderal Soedirman Jl. dr. Soeparno No. 63 Purwokerto, Kabupaten Banyumas Kode Pos 53122
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BioEksakta : Jurnal Ilmiah Biologi Unsoed
Published by Universitas Jenderal Soedirman
ISSN : -     EISSN : 27148564     DOI : -
Core Subject : Health, Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Veterinary
Jurnal BioEksakta menerbitkan artikel ilmiah hasil penelitian dalam bidang biologi umum termasuk Ekologi dan konservasi, Taksonomi dan Struktur, Biogeografi, Evolusi, Biodeversitas, Fisiologi dan Reproduksi, Biologi sel, Biologi Molekuler dan Genetika.
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)
Articles 14 Documents
 1   2 
Search results for , issue "Vol 3 No 1 (2021): BioEksakta" : 14 Documents clear
Isolasi dan Karakterisasi Cendawan Pathogen Daun Jagung Manis (Zea mays) Varietas Talenta menggunakan Metode Direct Plating Dan Moist Chamber di BBPP Ketindan, Jawa Timur nurul fadilah; Yuni Sri Rahayu; Lutfi Tri Andriani
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 3 No 1 (2021): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2020.2.3.3777

Abstract

Market demanding for corn increases with the years. The greatest request of all types of maize is the sweet corn (Zea mays) talenta variety. However, domestic productions arenot sufficient for this corn. This is due to problems such as corn disease which drastically derailed yields. This disease comes from the category of pathogenic fungi. This study aims to isolate and characterize fungal pathogens in sweet corn leaves (Zea mays) of talenta varieties taken from agricultural land, Ketindan Agricultural Training Center, East Java. This research is an experimental study using Completely Randomized Design (CRD). The insulation method used direct plating and moist chamber. Characterization and identification based on macroscopic characters include: old colony shape, old colony color, old colony texture, young colony shape, young colony color, and young colony texture. Microscopic characters include hyphal wall, type of hyphae branching, presence or absence of septa, presence or absence of clamp connections, sporangium shape, sporangium color, spore shape, spore color. The results of the direct plating method obtained 5 species of pathogenic fungi, including: Helminthosporium sp., Rhizoctonia sp., Peronosclerospora spp., Puccinia sp., And Trichometaspheria sp., While the moist chamber method obtained 2 species of pathogenic fungi, namely: Helminthosporium sp. and Rhizoctonia sp.
Variasi Marga Plumeria berdasarkan Karakter Morfologi Pollen di Wilayah Purwokerto Banyumas Jawa Tengah Sukarsa Sukarsa; wiwik herawati
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 3 No 1 (2021): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2020.2.3.4010

Abstract

The Plumeria clan is a member of the Apocynaceae family and has variations in each type. This study aims to determine the variation in morphological characters of the Plumeria genus pollen in the Purwokerto region. The results showed that the pollen morphology of 6 samples, namely Plumeira rubra, Plumeira rubra f angutifolia, Plumeira rubra f frangifani, Plumeira obtusu, Plumeira alba and Plumeira pudica have similarities in the type of aperture, namely tricolpate type and psilate type ornamentation. While the comparative P / E index The mean length of the polar axis (P) and the diameter of the equatorial plane (E) P. rubra, P. rubra F frangifani and P obtusus have the form of Oblate Spheroidal type pollen with P / E index 0.92 - 0.94 while Plumeira alba and Plumeira pudica Suboblate pollen form with P / E index 0.82 - 0.85
Keanekaragaman Kupu-Kupu Di Kawasan Air Terjun Dlundung Trawas, Kabupaten Mojokerto Jawa Timur Indonesia Siti Zulaikha; Lu'luil Fajriyatil Aliyah
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 3 No 1 (2021): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2020.2.3.4065

Abstract

The purpose of this study is to find out the diversity of butterflies in the area of Dlundung Trawas Waterfall, Mojokerto, East Java. The embedding method uses Visual Day Flaying by following the path in the area, the data obtained from observations are analyzed using the analysis of diversity index and Relative Abundance. Obtained as many as 22 species with a value of H'= 2.61 and the highest species FR are Libythea myrrha, Letopsia nina, Taencia palguna, and Papilio memnon. Based on these results it is known that the diversity of butterflies in the region in the moderate category due to the support of biotic and abiotic factors.
Kelimpahan Tungau Predator pada Tanaman Melati Gambir (Jasminum officinale) di Desa Cipawon, Bukateja, Purbalingga, Jawa Tengah Ika Rakhmayani; Bambang Heru Budianto; Trisnowati Budi Ambarningrum
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 3 No 1 (2021): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2020.2.3.3927

Abstract

The Gambier Jasmine plant (Jasminum officinale) is located in Bukateja District, Cipawon Village, Purbalingga, Central Java is a commodity with high economic value, its use a tea fragrance, cosmetics, perfumes dan others. One of the cause of decreased productivity of Gambier Jasmine is ude to the presence of pets, namely mites. The pest mite population is controlled by predatory mites. Therefore, this study aimed to determne the types of predatory mites and determine the abundance of predatory mites in Gambier Jasmine (J. officinale) in Cipawon Village, Purbalingga, Central Java. This research used survey method with purposive sampling technique. There are nine sampling points, eight pointa on the outer edge and one point in the middle of plantation. Each sampling is represented by three trees. The sample in the form of Gambier Jasmine Leaves came form the lowest ten leaf stalks in one tree at each sampling point. The number of leaf samples obtained was 270 leaf samples. Each sampling point was taken three times with one week intervals. The variable observed include the number of individuals and the number of species of predatory mites on the Gambier Jasmine (J. officinale) and the parameters calculated were leaf area, leaf sitting angle, length and density of trichomes, temperature, humidity, asa well as rainfall. The data obtained were analyzed by analysis of variance (ANOVA) with an error rate of 20%. The result of the identification of predatory mites on the Gambier Jasmine plant were obtained by two species, namely Amblyseius largoensis and Phytoseius amba. A. largoensis as many as 76 individuals with an abundance percentage of 61,8% and P. amba as many as 47 individuals with an abundance percentage of 38,2%. The abundance of predatory mites A. largoensis and P. amba is classified as moderate because predators are easy to find, every three leaves there is one predator. Key words: abundance., Cipawon., Gambier Jasmine., predatory mites.
Detection Moleculer Of Putative 18S rRNA Gen Protozoa Trichodina sp. Infected Larvae Gurami (Osphronemus gouramy L) in Balai Benih Ikan Kutasari Purbalingga Central Java Rokhmani Rokhmani; Daniel Joko Wahyono; Lilis Mulyani
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 3 No 1 (2021): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2020.2.3.3924

Abstract

Trichodina spp. are ectoparasitic pathogens of ciliata group that commonly infect both freshwater and marine fish, including gouramy fish. As a result of infection of Trichodina spp. this will lead to inhibition of fish growth and decreased fish production, resulting in low fish selling value. The rate of occurrence of Trichodina spp. that infects gurami can reach 100%. Research has been conducted to determine which one Trichodina spp. Protozoa that infects the gouramy seeds of BBI (Fish Seed Center) Kutasari Purbalingga following detection of 18S RNA gene. Gene detection method used in this research is Polymerase Chain Reaction (PCR) is a technique of DNA synthesis and amplification in vitro. This research is done following these methodes: (1) sampling of Gurami fish with purposive sampling which obtained from BBI Kutasari Purbalingga, (2) isolation of Trichodina spp., (3). Preparation of Trichodina spp. sample and its identification, and (4). Molecular character obervation following detection of 18S rRNA gene. This study obtained 10% percentage of detection of 18S rRNA genes of the species of Trichodina paraheterodentata that infect on the gouramy fish of Purbalingga. The percentage rate of detection of these genes is low when compared with the results of the detection of 18S rRNA Trichodina paraheterodentata gene that infects gouramy fish in Banjarnegara.
Keragaman dan Intensitas Kutu Parasit (Ordo: Phthiraptera) pada Ayam Kampung (Gallus gallus domesticus) imam widhiono; Viola Firstrianti Salsabila; edy riwidiharso riwidiharso
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 3 No 1 (2021): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2020.2.3.3868

Abstract

Abstract Phthiraptera lice is the common ectoparasite that attacks domestic chickens. Parasitic lice infestation is not lethal, but it can seriously disrupt the growth of chickens. The purpose of this study was to determine the diversity of lice species infecting domestic chickens in five villages in Purwokerto and its surroundings and to determine the infestation intensity of each species of lice infecting the body parts of domestic chickens in five villages in Purwokerto and its surroundings. This research was conducted using survey method and random sampling techniques in five villages in Purwokerto and its surroundings, namely Kedungwuluh Village, Kedungwringin Village, Kutasari Village, Karangsalam Village, and Karanggintung Village. Lice were sampled from five female and male domestic chickens in each village. Lice sampling is carried out on the head, wings, thighs, chest and legs of the chickens. Lice samples were then observed and identified under a microscope in the Laboratory of Entomology and Parasitology, Faculty of Biology, Unsoed. Lice sample data found in chickens from each location and body part were analyzed for variance using software SPSS 16 and using the Shannon-Wiener diversity index. The identification results showed that there were three species of lice species (order Phthiraptera) which were found infesting domestic chickens (Gallus gallus domesticus) in five villages in Purwokerto and its surroundings, namely Menopon gallinae, Lipeurus caponis, and Menacanthus cornutus. The three species of lice were the member of two families (Menoponidae and Philopteridae). Species diversity based on the Shannon-Wiener diversity index shows location I (H´: 0.914), location II (H´: 0.693), location III (H´: 1.066), location IV (H´: 1.081), and location V (H ´: 0.878). Interpretation from all sites indicated that sites I, III, and V had low species diversity, while sites II and IV had moderate species diversity. The intensity of lice infestation on domestic chickens (Gallus gallus domesticus) was 16.82%. The intensity of each type of lice in domestic chickens (Gallus gallus domesticus) was M. cornutus (32%), M. gallinae (30,9%), and L. caponis (21,2%).
Identifikasi Jamur Patogen dan Tingkat Persentase Penyakit pada Tanaman Selada (Lactuca sativa L.) di Sentra Tanaman Sayur Desa Serang, Kecamatan Karangreja, Kabupaten Purbalingga Agustina Nursanti; Eddy Tri Sucianto; Aris Mumpuni
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 3 No 1 (2021): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2020.2.3.3396

Abstract

Selada (Lactuca sativa L.) merupakan tanaman sayuran daun dari family Compositae (Asteraceae) yang mempunyai nilai ekonomis tinggi. Selada mengandung banyak mineral dan vitamin yang berguna untuk kesehatan. Tanaman selada memiliki daun yang berombak, bergerigi, berwarna hijau segar dan ada juga yang berwarna merah. Terdapat beberapa kendala dalam budidaya tanaman selada diantarnya adalah gangguan hama dan penyakit seperti siput, nematoda, busuk daun, dan busuk akar. Penelitian ini bertujuan untuk mengetahui jenis jamur patogen penyebab penyakit dan tingkat persentase penyakit pada tanaman Selada di Desa Serang, Kecamatan Karangreja, Kabupaten Purbalingga. Penelitian ini menggunakan metode survey dengan pengambilan sampel yang dilakukan secara purposive random sampling di dua lokasi yang berbeda. Proses identifikasi dilakukan dengan pengamatan secara makroskopis dan mikroskopis. Hasil pengamatan dideterminasi dengan buku identifikasi dan dilanjutkan dengan uji Postulat Koch. Hasil penelitian di Sentra Tanaman Sayur Desa Serang, Kecamatan Karangreja, Kabupaten Purbalingga, didapatkan jenis penyakit selada adalah penyakit busuk daun (bottom rot) dan bercak daun. Penyakit busuk daun disebabkan oleh jamur Rhizoctonia sp. dan penyakit bercak daun disebabkan oleh jamur Nigrospora sp. Penyakit yang paling banyak muncul pada tanaman selada yaitu penyakit busuk daun oleh Rhizoctonia sp. dengan frekuensi kemunculan sebanyak 206 kali dan rata-rata persentase penyakit sebesar 52,79%.
Identifikasi Jamur Patogen dan Tingkat Persentase Penyakit pada Tanaman Selada (Lactuca sativa L.) di Sentra Tanaman Sayur Desa Serang, Kecamatan Karangreja, Kabupaten Purbalingga Nursanti, Agustina; Sucianto, Eddy Tri; Mumpuni, Aris
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 3 No 1 (2021): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2020.2.3.3396

Abstract

Selada (Lactuca sativa L.) merupakan tanaman sayuran daun dari family Compositae (Asteraceae) yang mempunyai nilai ekonomis tinggi. Selada mengandung banyak mineral dan vitamin yang berguna untuk kesehatan. Tanaman selada memiliki daun yang berombak, bergerigi, berwarna hijau segar dan ada juga yang berwarna merah. Terdapat beberapa kendala dalam budidaya tanaman selada diantarnya adalah gangguan hama dan penyakit seperti siput, nematoda, busuk daun, dan busuk akar. Penelitian ini bertujuan untuk mengetahui jenis jamur patogen penyebab penyakit dan tingkat persentase penyakit pada tanaman Selada di Desa Serang, Kecamatan Karangreja, Kabupaten Purbalingga. Penelitian ini menggunakan metode survey dengan pengambilan sampel yang dilakukan secara purposive random sampling di dua lokasi yang berbeda. Proses identifikasi dilakukan dengan pengamatan secara makroskopis dan mikroskopis. Hasil pengamatan dideterminasi dengan buku identifikasi dan dilanjutkan dengan uji Postulat Koch. Hasil penelitian di Sentra Tanaman Sayur Desa Serang, Kecamatan Karangreja, Kabupaten Purbalingga, didapatkan jenis penyakit selada adalah penyakit busuk daun (bottom rot) dan bercak daun. Penyakit busuk daun disebabkan oleh jamur Rhizoctonia sp. dan penyakit bercak daun disebabkan oleh jamur Nigrospora sp. Penyakit yang paling banyak muncul pada tanaman selada yaitu penyakit busuk daun oleh Rhizoctonia sp. dengan frekuensi kemunculan sebanyak 206 kali dan rata-rata persentase penyakit sebesar 52,79%.
Isolasi dan Karakterisasi Cendawan Pathogen Daun Jagung Manis (Zea mays) Varietas Talenta menggunakan Metode Direct Plating Dan Moist Chamber di BBPP Ketindan, Jawa Timur fadilah, nurul; Rahayu, Yuni Sri; Andriani, Lutfi Tri
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 3 No 1 (2021): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2020.2.3.3777

Abstract

Permintaan pasar akan jagung meningkat dengan seiring berjalannya tahun. Permintaan terbesar dari semua jenis jagung adalah jagung manis (Zea mays) varietas talenta. Namun, produksi dalam negeri tidak mencukupi permintaan jagung tersebut. Hal ini dikarenakan adanya masalah seperti, penyakit jagung yang menggagalkan hasil panen secara drastis. Penyakit ini disebabkan oleh cendawan patogen. Penelitian ini bertujuan untuk mengisolasi dan mengkarakterisasi cendawan patogen pada daun jagung manis (Zea mays) varietas Talenta yang diambil dari Lahan Pertanian, Balai Besar Pelatihan Pertanian (BBPP) Ketindan, Jawa Timur. Penelitian ini merupakan penelitian eksperimental menggunakan Rancangan Acak lengkap (RAL). Metode isolasi cendawan yang digunakan adalah direct plating dan moist chamber. Karakterisasi dan Identifikasi berdasarkan karakter makroskopis meliputi: bentuk koloni tua, warna koloni tua, tekstur koloni tua, bentuk koloni muda, warna koloni muda, dan tekstur koloni muda. Karakter mikroskopis meliputi dinding hifa, tipe percabangan hifa, ada tidaknya septa, ada tidaknya sambungan apit (clamp connection), bentuk sporangium, warna sporangium, bentuk spora, warna spora). Hasil isolasi cendawan dari metode direct plating didapatkan 5 spesies cendawan patogen antara lain: Helminthosporium sp., Rhizoctonia sp., Peronosclerospora spp., Puccinia sp., dan Trichometaspheria sp., sedangkan metode moist chamber didapatkan 2 spesies cendawan patogen yaitu: Helminthosporium sp. dan Rhizoctonia sp.
Keragaman dan Intensitas Kutu Parasit (Ordo: Phthiraptera) pada Ayam Kampung (Gallus gallus domesticus) widhiono, imam; Salsabila, Viola Firstrianti; riwidiharso, edy riwidiharso
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 3 No 1 (2021): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2020.2.3.3868

Abstract

Ektoparasit yang menyerang ayam kampung biasanya terdiri atas kutu ordo Phthiraptera. Serangan kutu parasit tidak mematikan tetapi sangat mengganggu pertumbuhan ayam. Tujuan penelitian ini adalah untuk mengetahui keragaman spesies kutu yang menginfeksi ayam kampung pada lima desa di Purwokerto dan sekitarnya dan mengetahui intensitas infestasi tiap spesies kutu yang menginfeksi bagian tubuh ayam kampung pada lima desa tersebut. Penelitian ini dilakukan dengan metode survei, teknik pengambilan sampel secara acak pada lima desa di Purwokerto dan sekitarnya yaitu Desa Kedungwuluh, Desa Kedungwringin, Desa Kutasari, Desa Karangsalam, dan Desa Karanggintung. Setiap desa diambil lima ayam kampung betina dan lima ayam kampung jantan. Pengambilan sampel kutu dilakukan pada bagian kepala, sayap, paha, dada, dan kaki. Sampel kutu selanjutnya diamati dan diidentifikasi di bawah mikroskop di laboratorium Entomologi dan Parasitologi Fakultas Biologi Unsoed. Data sampel kutu yang ditemukan pada ayam dari tiap lokasi dan bagian tubuh dianalisis varian menggunakan software SPSS 16 dan menggunakan indeks keanekaragaman Shannon-Wiener. Hasil identifikasi menunjukkan adanya tiga spesies kutu (ordo Phthiraptera) yang ditemukan menginfestasi ayam kampung (Gallus gallus domesticus) pada lima desa di Purwokerto dan sekitarnya, yaitu Menopon gallinae, Lipeurus caponis, dan Menacanthus cornutus. Ketiga spesies kutu tersebut termasuk ke dalam dua familia (Menoponidae dan Philopteridae). Keragaman spesies berdasarkan indeks diversitas Shannon-Wiener menunjukkan lokasi I (H´: 0,914), lokasi II (H´: 0,693), lokasi III (H´: 1,066), lokasi IV (H´: 1,081), dan lokasi V (H´: 0,878). Interpretasi dari semua lokasi menunjukkan bahwa lokasi I, III, dan V memiliki keragaman spesies rendah, sedangkan lokasi II dan IV memiliki keragaman spesies sedang. Intensitas serangan kutu pada ayam kampung (Gallus gallus domesticus) adalah 16,82%. Intensitas dari masing-masing jenis kutu pada ayam kampung (Gallus gallus domesticus) adalah M. cornutus (32%), M. gallinae (30,9%), dan L. caponis (21,2%).

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