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Arif Rahman Hikam
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Fakultas Biologi Universitas Jenderal Soedirman Jl. dr. Soeparno No. 63 Purwokerto, Kabupaten Banyumas Kode Pos 53122
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BioEksakta : Jurnal Ilmiah Biologi Unsoed
Published by Universitas Jenderal Soedirman
ISSN : -     EISSN : 27148564     DOI : -
Core Subject : Health, Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Veterinary
Jurnal BioEksakta menerbitkan artikel ilmiah hasil penelitian dalam bidang biologi umum termasuk Ekologi dan konservasi, Taksonomi dan Struktur, Biogeografi, Evolusi, Biodeversitas, Fisiologi dan Reproduksi, Biologi sel, Biologi Molekuler dan Genetika.
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)
Articles 14 Documents
 1   2 
Search results for , issue "Vol 3 No 4 (2021): BioEksakta" : 14 Documents clear
The Effect Of Date Palm Extract (Phoenix dactylifera L.) On Orchid Plantlets (Vanda tricolor Lind) Growth using In Vitro Culture Resita Nursechan; kamsinah kamsinah; Iman Budisantoso
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 3 No 4 (2021): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2021.3.4.4227

Abstract

Vanda tricolor Lindl. is widespread in Java, Bali, and Sulawesi, V. tricolor on natural habitat became scarce due to forest destruction and natural disasters and human activities. To prevent the extinction it can be done by the propagation of orchids using in vitro culture. The addition of dates extract (Phoenix dactylifera L.) containing carbohydrates to increase growth and differentiation of cells in orchids. This study aimed to determine the effect of date palm extract on the growth of V. tricolor L. plants and determine the best dose of date palm extract on the growth of V. tricolor plantlets. This research was conducted experimentally with a Completely Randomized Design (CRD). The addition of dates on Vaccint & Went / VW media consisting of 6 treatments, that are : 0 g/L, 50 g/L, 100 g/L, 150 g/L, 200 g/L, 250 g/L, each treatment repeated 3 times so there are 18 trial units. The source of the explants used was orchid plantlet (V. tricolor L.). The parameters include the number of roots, root length, number of leaves, plantlet height, and leaf length. The research data has been analyzed using Analysis of variance (ANOVA) with a confidence level of 95% and 99%. The result of this research showed that the addition of date palm extract give a result not significant for the number of leaves, the number of roots, the longest leaf length, the longest root length, the plantlet height of the vanda tricolor orchid in vitro.
Aktivitas Amilolitik Bakteri Sedimen Mangrove Pantai Logending, Ayah, Kebumen pada Suhu dan pH Berbeda Alfiani Rahmawati; Oedjijono .; Dini Ryandini
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 3 No 4 (2021): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2021.3.4.4574

Abstract

The main purpose of this study wereto determine the ability of mangrove sediment bacterial isolates to produce amylase, to determine the optimum pH and temperature on the amylase activity, and to identify theamylase-producing bacteria. The research was conducted by survey method. The research stages included, screening of amylolytic bacteria, growth curvespreparation, amylase production, optimizing amylolytic activity at varies oftemperature (35ºC; 36ºC; 37ºC; 38ºC) and pH (4.5; 5; 5.5; 6) and characterization of amylolytic isolates. Parameters measured were amylolytic index, amylase enzyme activity unit, total bacterial unit, and identity of amylolytic bacteria from mangrove sediments. Data analysis was done descriptively. The results showed that the highest amylolytic index were shown by isolate LG113 with an amylolytic index value of 9,86 mm from 10 amylolytic isolates from mangrove sediments. The optimum temperature of amylase activity was 37ºC(2,13 U/mL) and the optimum pH was 6(2,14 U/mL). The total number of amylolytic bacterial cells at the end of production time was 1,94x1013 CFU/mL. The identity of isolates of amylolytic bacteria from mangrove sediments at the Logending beachwas belong toBacillus genus.
Kekayaan Jenis Ikan Elasmobranchii di TPI Bajomulyo Kabupaten Pati dan Status Konservasinya Ning Setiati; Khoirinida Suftiyani Putri
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 3 No 4 (2021): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2021.3.4.3479

Abstract

Tingkat kekayaan jenis biota laut di Indonesia sangat beragam baik dari jenis ikan bertulang sejati maupun ikan bertulang rawan (Elasmobranchii). Ikan Elasmobranchii saat ini sedang menghadapi masalah tingginya laju kepunahan akibat penangkapan berlebih (overfishing). Berdasarkan IUCN red list terdapat 1 jenis hiu masuk ke dalam status Kritis Punah (Critically Endangered), 5 spesies berstatus Terancam Punah (Endangered), 23 Rentan (Vulnarable), sedangkan 35 spesies lainnya pada status hampir terancam (Near Threatened) di Indonesia. Penelitian bertujuan untuk mengidentifikasi dan mendeskripsikan jenis serta status konservasi ikan Elasmobranchii yang didaratkan di TPI Bajomulyo. Metode penelitian menggunakan teknik observasi dan wawancara. Pengambilan data dilakukan pada bulan Maret sampai bulan April 2020. Penentuan jenis dianalisis secara deskriptif eksploratif berdasarkan hasil identifikasi ciri-ciri yang diamati. Terdapat 11 spesies ikan Elasmobranchii yang ditemukan selama proses penelitian. Spesies tersebut antara lain Carcharhinus sealei, Chiloscyllium punctatum, Dasyatis kuhlii, Himantura gerrardi, Himantura undulata, Himantura jenkinsii, Pastinachus sephen, Taeniura meyeni, Gymnura poecilura, Rhynchobatus australiae, dan Rhina ancylostoma. Penentuan status konservasi yang digunakan dalam penelitian ini antara lain International Union for Conservation of Nature (IUCN), Convention on International Trade in Endangered Species of Wild Fauna and Flora (CITES), PP Nomor 8 Tahun 1999, UU Nomor 5 Tahun 1990 dan Peraturan Menteri LHK Nomor P.106 Tahun 2018.
Optimasi Konsentrasi Glukosa dan Waktu Inkubasi Terhadap Bobot β-Glukan Jamur Schizophyllum commune Tri Rahayu Apriyani; Nuraeni Ekowati; Nuniek Ina Ratnaningtyas
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 3 No 4 (2021): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2021.3.4.4601

Abstract

The main purpose of this study was to determine the effect of the interaction between glucose concentration and incubation time on the growth of fungi S. commune and also the weight of β-glucan was used to determine the best glucose concentration and incubation time for the growth of fungi S. commune and β-glucan production. The research was conducted by experimental method of completely randomized factorial design (CRD factorial) with two factors carried out in three repetitions. The treatments provided included variations in glucose concentration (G) with three levels, namely 10 g/L (G1), 20 g/L (G2), and 30 g/L (G3), and variations in incubation time (W) with three levels, namely incubation time of 20 days (I1). , incubation time of 25 days (I2), and incubation time of 30 days (I3). Variables which used in this research independent and dependent variables. The independent variables were glucose concentration and incubation time, while the dependent variables were fungal mycelium growth and β-glucan production. The main parameter observed was β-glucan weight. The supporting parameters were dry biomass weight and the final pH medium. Data analysis was performed by Analysis of Variance (ANOVA) at 95% accuracy levels, followed by Duncan's test (Ducan Multiple Range Test). The results showed that glucose concentration and incubation time were significantly affected to the growth and production of β-glucan fungus S. commune. Glucose concentration of 30 g/L and incubation time of 25 days was the optimum glucose concentration and incubation time for the growth of S. commune fungus, and also glucose concentration of 30 g/L and incubation time of 25 days was the optimum glucose concentration and incubation time for β-glucan production of S. commune.
Biodegradasi Bioplastik Berbasis Pati Menggunakan Isolat Fungi Indigenous Asal Tempat Pembuangan Akhir Gunung Tugel, Kabupaten Banyumas Jimmy Al Fa'is; Ratna Stia Dewi; Ajeng Arum Sari
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 3 No 4 (2021): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2021.3.4.4605

Abstract

The use of plastic in Indonesia, especially as packaging, is very popular among the public, because it is practical and has many uses. Plastic consumption in 2020 even reached 67.8 million tons per day. Therefore, one way to overcome this problem is to use and produce biodegradable plastics or bioplastics. Biodegradation of bioplastics can be done with fungi. The potential place to find bioplastic degrading fungi is the Gunung Tugel Ex-Final Disposal Site (TPA). Considering the fact that bioplastiks have a fairly good level of degradation, in this study a starch-based biodegradation test will be carried out using isolats of indigenous fungi originating from Ex-TPA Gunung Tugel using the method of calculating the weight loss of bioplastics (weight loss). This study aims to obtain starch-based bioplastic degrading fungi isolats from Ex-TPA Gunung Tugel, Banyumas Regency and determine the highest biodegradability ability of starch-based bioplastics by fungal isolats from Ex-TPA Gunung Tugel, Banyumas Regency. The results showed that isolats of indigenous fungi that had the potential to degrade starch-based bioplastics from Ex-TPA Gunung Tugel, Banyumas Regency were obtained from the genus Aspergillus (GT2 isolate) and Penicillium (GT1, GT3, GT4, GT5, and GT6) isolate. The highest biodegradability of starch-based bioplastics was found by fungi from the genus Aspergillus, namely isolate GT2 with a weight loss of 21.84%. The biodegradation occurs due to the activity of endoamylase, exoamylase, and glucanase enzymes produced by fungi. The observation of the texture of the bioplastic resulted in a significant change in color, namely from the white bioplastic sheet to brownish. These observations also showed the attachment of fungal mycelium on the surface of the bioplastik accompanied by a hollow and uneven surface of the bioplastikc. Keywords : Biodegradation, Starch-based bioplastik, Indigenous Fungi, TPA.
Efek Pemberian Ekstrak Umbi Kembang Sungsang (Gloriosa superba L.) Pada Pembentukan Planlet Pisang (Musa paradisiaca L.) Kultivar Kepok Kunig Secara In Vitro Julaiha Wahyu; Eti Ernawiati; Tundjung Tripeni Handayani; Sri Wahyuningsih
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 3 No 4 (2021): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2021.3.4.4377

Abstract

The world community, including Indonesia much like bananas because it has a relatively low price and good nutritional content. Banana kepok is a type of banana favored by the public because it tastes good and sweet. However, the obstacle that is often faced in kepok bananas is the difficulty in providing quality banana seeds quickly. Therefore, alternative technology is needed through tissue culture techniques. The purpose of this study was to determine the effect of adding breech flower tuber extract in culture media on the formation of banana plantlets of yellow kepok cultivar and to obtain scientific information about the growth profile of yellow kepok plantlets due to the addition of breech flower tuber extract to culture media. This research was conducted at the MTC Laboratory, PT. Great Giant Pineapple PG 4 Labuhan Ratu, East Lampung from December 2019 - April 2020 using a Completely Randomized Design (CRD) with ten replications consisting of the addition of 10% breech flower tuber extract as the primary treatment, 0.1% pure colchicine as a positive control, and without addition as a negative control. The results showed that the addition of 10% breech flower tuber extract in the culture media caused the growth of yellow kepok banana plantlets to be slower than the addition of 0.1% colchicine. Then in the media, with the addition of 10% breech flower tuber extract, the number of shoots, shoot height, number of better root and leaf area than the media without expansion, but 0.1% colchicine still showed the best results.
Respon Pertumbuhan dan Fisiologis Bayam (Amaranthus tricolor) yang Dipapar Timbal pada Sistem Hidroponik Sumbu Eka Fitriani Yuspiah; Yeni Rahayu; Miftahuddin Miftahuddin
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 3 No 4 (2021): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2021.3.4.4767

Abstract

Abstract Green amaranth (Amaranthus tricolor) is a popular vegetable in Indonesia as a source of vitamins and minerals. However, this plant is susceptible to the contamination of heavy metals such as lead. This study aimed to analyze the growth responses and morphophysiological of A. tricolor in response to the application of lead in the forms of (Pb(NO3)2 using a wick hydroponic system. This study used a single factor randomized design with four treatments of lead level, i.e. 0 ppm, 1 ppm, 3 ppm, and 5 ppm, repeated 2 times. Data were analyzed by analysis of one-way ANOVA and post hoc test by Duncan in P-value 5%. The growth responses were observed by measuring the plant height, the number of leaves, leaf color, shoot and roots fresh weight, also its dry weight. Whereas, the physiological responses were observed by analyzing the plant chlorophyll levels. The results obtained that the applications of (Pb(NO3)2 decreased plant height, leaf number, fresh weight of shoot and roots, dry weight of shoot and roots, also the chlorophyll levels. These lead treatments were affecting root loss, changed the color of leaves to become yellowish-green, and showing yellow-spotted close to the midrib. Analysis of lead uptake in stems and leaves showed that the absorption value increased along with the applications of (Pb(NO3)2 in the treatment. The treatment of 5 ppm of (Pb(NO3)2 showed the lowest result on all parameters. Key Words : Amaranthus tricolor, Wick Hydroponic System, The Leads, Metal Pollution.
The Effect of Date Palm Extract (Phoenix dactylifera L.) on Orchid Plantlets (Vanda tricolor Lind.) Growth Using In Vitro Culture Nursechan, Resita; Kamsinah, Kamsinah; budisantoso, iman
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 3 No 4 (2021): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2021.3.4.6874

Abstract

Vanda tricolor Lindl. is widespread in Java, Bali, and Sulawesi, V. tricolor on natural habitat became scarce due to forest destruction and natural disasters and human activities. To prevent the extinction it can be done by the propagation of orchids using in vitro culture. The addition of dates extract (Phoenix dactylifera L.) containing carbohydrates to increase growth and differentiation of cells in orchids. This study aimed to determine the effect of date palm extract on the growth of V. tricolor L. plants and determine the best dose of date palm extract on the growth of V. tricolor plantlets. This research was conducted experimentally with a Completely Randomized Design (CRD). The addition of dates on Vaccint & Went / VW media consisting of 6 treatments, that are : 0 g/L, 50 g/L, 100 g/L, 150 g/L, 200 g/L, 250 g/L, each treatment repeated 3 times so there are 18 trial units. The source of the explants used was orchid plantlet (V. tricolor L.). The parameters include the number of roots, root length, number of leaves, plantlet height, and leaf length. The research data has been analyzed using Analysis of variance (ANOVA) with a confidence level of 95% and 99%. The result of this research showed that the addition of date palm extract give a result not significant for the number of leaves, the number of roots, the longest leaf length, the longest root length, the plantlet height of the vanda tricolor orchid in vitro.
Kekayaan Jenis Ikan Elasmobranchii di TPI Bajomulyo Kabupaten Pati dan Status Konservasinya Setiati, Ning; Putri, Khoirinida Suftiyani
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 3 No 4 (2021): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2021.3.4.3479

Abstract

The wealth of marine biota species in Indonesia are very diverse, both from bony fish and cartilaginous fish (Elasmobranchii). The Elasmobranchii fish are currently facing an extinction rate problem due to overfishing. Based on the IUCN Red List, 1 shark species is classified as Critically Endangered, 5 shark species are Endangered, 23 sharks species are Vulnerable and 35 other sharks species are in Near Threatened in Indonesia. The research aims to identify, describe the type and conservation status of Elasmobranchii fish caught at TPI Bajomulyo. The research method uses observation and interview techniques. Data were collected from March to April 2020. The determination of the type was analyzed descriptively based on the results of the observed assessment characteristics. The determination of the conservation status used in this research include the International Union for Conservation of Nature (IUCN), Convention on International Trade in Endangered Species of Wild Fauna and Flora (CITES), Indonesian Government Regulation Number 8 Year 1999, Constitution Number 5 Year 1990 and Ministerial Regulation of Environment and Forestry Number P.106 Year 2018. There are 11 species of Elasmobranchii fish found during the research process. These species include Carcharhinus sealei (NT), Chiloscyllium punctatum (NT), Dasyatis kuhlii (DD), Himantura gerrardi (VU), Himantura undulata (VU), Himantura jenkinsii (VU), Pastinachus sephen (NT), Taeniura meyeni (VU), Gymnura poecilura (NT), Rhynchobatus australiae (CR), and Rhina ancylostoma (CR). Based on CITES, there are only 2 species categorized as Appendix II, Rhynchobatus australiae and Rhina ancylostoma. This research provides benefits for fisherman and related agencies to pay attention to catching protected fish so that it is sustainability is maintained.
Efek Pemberian Ekstrak Umbi Kembang Sungsang (Gloriosa superba L.) Pada Pembentukan Planlet Pisang (Musa paradisiaca L.) Kultivar Kepok Kunig Secara In Vitro Wahyu, Julaiha; Ernawiati, Eti; Handayani, Tundjung Tripeni; Wahyuningsih, Sri
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 3 No 4 (2021): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2021.3.4.4377

Abstract

The world community, including Indonesia much like bananas because it has a relatively low price and good nutritional content. Banana kepok is a type of banana favored by the public because it tastes good and sweet. However, the obstacle that is often faced in kepok bananas is the difficulty in providing quality banana seeds quickly. Therefore, alternative technology is needed through tissue culture techniques. The purpose of this study was to determine the effect of adding breech flower tuber extract in culture media on the formation of banana plantlets of yellow kepok cultivar and to obtain scientific information about the growth profile of yellow kepok plantlets due to the addition of breech flower tuber extract to culture media. This research was conducted at the MTC Laboratory, PT. Great Giant Pineapple PG 4 Labuhan Ratu, East Lampung from December 2019 - April 2020 using a Completely Randomized Design (CRD) with ten replications consisting of the addition of 10% breech flower tuber extract as the primary treatment, 0.1% pure colchicine as a positive control, and without addition as a negative control. The results showed that the addition of 10% breech flower tuber extract in the culture media caused the growth of yellow kepok banana plantlets to be slower than the addition of 0.1% colchicine. Then in the media, with the addition of 10% breech flower tuber extract, the number of shoots, shoot height, number of better root and leaf area than the media without expansion, but 0.1% colchicine still showed the best results.

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