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Arif Rahman Hikam
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Fakultas Biologi Universitas Jenderal Soedirman Jl. dr. Soeparno No. 63 Purwokerto, Kabupaten Banyumas Kode Pos 53122
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BioEksakta : Jurnal Ilmiah Biologi Unsoed
Published by Universitas Jenderal Soedirman
ISSN : -     EISSN : 27148564     DOI : -
Core Subject : Health, Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Veterinary
Jurnal BioEksakta menerbitkan artikel ilmiah hasil penelitian dalam bidang biologi umum termasuk Ekologi dan konservasi, Taksonomi dan Struktur, Biogeografi, Evolusi, Biodeversitas, Fisiologi dan Reproduksi, Biologi sel, Biologi Molekuler dan Genetika.
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)
Articles 16 Documents
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Search results for , issue "Vol 4 No 1 (2022): Bioeksakta" : 16 Documents clear
Distribusi dan Sex Ratio Udang Macrobrachium pilimanus pada Sungai Mengaji di Banyumas Prodhiana, Prodhiana; Sastranegara, Moh. Husein; Winarni, Elly Tuti
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 4 No 1 (2022): Bioeksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2022.4.1.4578

Abstract

The one forms of freshwater ecosystems which is the habitat of various organisms is rivers. The Mengaji River is a river that springs from the southern slopes of Mount Slamet. Rivers always experience changes in physical, chemical, and biological factors. The decline in river water quality greatly affects the distribution and sex ratio of Macrobrachium pilimanus in the Mengaji River. This research aims to determine the distribution and sex ratio of M. pilimanus shrimp in the Mengaji River. The research was conducted using a survey method for determining stations and purposive random sampling for sampling. The samples obtained were then identified and measured using the morphometric method. Research was conducted between June-August 2020. Shrimp distribution data were analyzed using the "F" test with SPSS 2016, while the sex ratio of shrimp was analyzed using the sex ratio formula from the Kao and Liu method. The results of this study found that the distribution of M. pilimanus shrimp in the Mengaji River, Banyumas, was not evenly distributed between stations. The highest distribution was found at Station IV, while the highest female shrimp was found at Station II. The sex ratio of M. pilimanus shrimp in the Mengaji River Banyumas was not balanced and was dominated by females. The sex ratio was found to be the highest at Station II.
Keragaman Tumbuhan yang Dimanfaatkan Sebagai Pewarna Alami Batik Di Kabupaten Banyumas Sir, Makdalena Viviliani; Herawati, Wiwik; Sukarsa, Sukarsa
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 4 No 1 (2022): Bioeksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2022.4.1.4592

Abstract

Natural dyes are compounds that can be produced from various types of natural sources. Plants that produce natural dyes can be obtained from their parts such as leaves, bark, fruit skin, seeds, roots and flowers, which have gone through several processes, namely boiling, burning, crushing, pounding and immediately used. This study aims to determine the types of plants used as natural dyes for batik, and to determine which parts of plant organs are used as natural dyes for batik. The method used in this research is a survey method. Sampling was done by purposive sampling. Data were collected by observing and interviewing the batik home industry in Banyumas Regency. The parameters used are the types of plants and plant organs that are used as natural dyes for batik. Data were tabulated and identified for analysis. Analysis of the research data was carried out descriptively. Plant used as natural batik dyes in several Batik Home Industries in Banyumas Regency consists of 12 plant species the parts of plants were roots, stems, leaves, tubers, wood, bark, fruit skin, and coir. Natural dyes of batik are obtained by boiling and pounding. Then Used by Soaking, Dipping, and to draw motifs.
Pengaruh pH dan Waktu Inkubasi Berbeda Terhadap Pertumbuhan dan Produksi β-Glukan Schizophyllum commune Maharani, Amalia Sofia; Ekowati, Nuraeni; Ratnaningtyas, Nuniek Ina
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 4 No 1 (2022): Bioeksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2022.4.1.4594

Abstract

Schizophyllum commune is a fungus that grows naturally on tree trunks and wood processing waste. This mushroom contains β-glucan compounds which have the potential for drug development in the world of health. The main purpose of this study was to determine the optimum value of pH and incubation time on S. commune's growth, and also the optimum value of pH and incubation time on β-glucan production of S. commune. The research was conducted by experimental method of completely randomized factorial design (CRD factorial). The treatment given includes variations in pH (P) with three levels, namely pH 5 (P1), pH 6 (P2), and pH 7 (P3), and variations in incubation time (W) with three levels, namely incubation time of 20 days (W1), 25 days (W2), and 30 days (W3). . The independent variables were pH and incubation time, while the dependent variables were fungal mycelium growth and β-glucan production. The main parameter observed was β-glucan weight. The supporting parameters were dry biomass weight and the final pH medium. Data analysis was performed by Analysis of Variance (ANOVA) at 95% accuracy levels, followed by Duncan's test (Ducan Multiple Range Test). The results showed that pH and incubation time were significantly affected to the growth and production of β-glucan fungus S. commune. The value of pH 6 and incubation time of 25 days was the optimum condition for the growth of S. commune, and also pH 5 and incubation time of 25 days was the optimum condition for β-glucan production of S. commune.
Uji Viabilitas Polen Tanaman Kedelai (Glycine max (L.) Merril) pada berbagai Lama Penyimpanan Andini, Hastya Tri; Abbas, Muachiroh; kamsinah, Kamsinah
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 4 No 1 (2022): Bioeksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2022.4.1.4595

Abstract

Pollen can be used for plant identification because it has a distinctive structure and ornamentation, besides that it can also be used as a plant breeding agent as a contributor to male parental characters. The character of pollen as parental male is very important in plant breeding. One way to know the quality of pollen is to look at its viability. Pollen viability can be tested by staining method or by germination method. Pollen viability is known to be lost over a certain period of time. The purpose of this study was to determine the effect of storage time on viability of soybean pollen, and to determine the best storage period with the highest viability of soybean pollen. This research was conducted experimentally with a Completely Randomized Design (CRD) consisting of 4 treatments, namely P0, Control. P1, 7 days of storage. P2, 14 days of storage. P3, 21 days of storage. The independent variable is variation in storage time while the dependent variable is pollen viability. The parameters observed were the number of stained pollen, germination capacity, and the length of the germinating pollen tube as evidence that the pollen was viable. The research data obtained were analyzed using Analysis of Variance (ANOVA) with an error rate of 5%, then continued with the BNT test with an error rate of 5%. The results showed that the provision of storage time treatment on soybean pollen had an effect on pollen viability, namely by reducing pollen viability. The best shelf life to get the highest pollen viability in soybean pollen is 0 to 7 days after the sample is taken.
Histopatologi Hepar Mencit (Mus musculus) Jantan yang Diberi Air Rebusan Plastik terhadap Antioksidan Alami Safitri, Nuridha Audinia; Atang, Atang; Wibowo, Eko Setio
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 4 No 1 (2022): Bioeksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2022.4.1.4606

Abstract

Plastic in Indonesian daily life cannot be avoided anymore. Materials for the manufacture of plastic can cause free radicals to the body, thus affecting the immune system. These materials are including phthalates and bisphenol-A (BPA), which commonly called as plasticizers. Free radicals in the body can be inhibited with antioxidants. Antioxidants can be obtained from endogenous (inside the body) and exogenous (outside the body). The purpose of this study was to determine the damage level of liver histopathological structure as the result of plastic boiled water induction towards natural antioxidants feeding on male mice (Mus musculus). This study used an experimental method with a completely randomized design (CRD) consisting of six treatments and five replications for 21 days. The treatments included two control groups and four other groups treated with plastic boiled water and natural antioxidants. The independent variable is natural antioxidants and the dependent variable is the liver histopathology of male mice. The qualitative parameters observed were the liver histopathological structure of male mice is in the state of inflammatory cell infiltration, degeneration, and necrosis. The research data is an overview of the liver histopathological structure of male mice. The obtained data were analyzed using descriptive qualitative analysis method. Oral induction of boiled-plastic water can affect the changes of liver histological structure. Natural antioxidants given in male mice can maintain the tissue structure as the result of boiled-plastic water induction. The results showed the damage level of male mice liver histopathological structure as reversible degeneration.
Identifikasi dan Uji Potensi Amilolitik Isolat Jamur Pendegradasi Sampah Organik Haqq, Insaaniy Mahdiyatul; Dewi, Ratna Stia; Mumpuni, Aris; Hikam, Arif Rahman; Yulianti, Dwiana Muflihah
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 4 No 1 (2022): Bioeksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2022.4.1.4748

Abstract

Organic waste is composed of organic compounds. The accumulation of organic waste is a serious problem. Fungal have an important role in degrading organic waste in the composting process. Biodegradation of organic waste is closely related to fungal ability to hydrolyze starch. The purpose of this study was to know fungi isolate which hydrolyze starch from organic waste and amylolytic potential of the isolate. This research was conducted by survey and experimental method. Kitchen waste samples consist of food waste and other organic waste taken from homes in Bancarkembar, Bobosan, Grendeng, Karangwangkal, Pabuaran, Purwanegara, and Sumampir village. A screening test with Starch Agar medium was used to know amylolytic potential of the isolates. Result showed there were eight isolates which have potential to hydrolyze starch. Six isolates which have higher amylolitic index were identified as Fusarium sp., Aspergillus sp., and Penicillium sp. Furthermore, to determine the amylolytic activity quantitatively, the DNS method was used to measure glucose levels. Fusarium sp. had the highest starch degradation activity with the average glucose content of the medium Fusarium sp. as much as 3,568.63 ppm.

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