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INDONESIA
HAYATI Journal of Biosciences
Published by Institut Pertanian Bogor
ISSN : 19783019     EISSN : 20864094     DOI : -
Core Subject : Science, Agriculture,
Agriculture, Biological Sciences & Forestry Earth & Planetary Sciences
HAYATI Journal of Biosciences (HAYATI J Biosci) publishes articles and short communication in tropical bioscience fields such as development, biotechnology, biodiversity and environmental issues. HAYATI J Biosci covers wide range of all life forms topics including virus, microbes, fungi, plants, animal and human. HAYATI J Biosci has been also indexed/registered in Crossref, DOAJ, CABI, EBSCO, Agricola and ProQuest.
Arjuna Subject : -
Articles 1,091 Documents
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First Record of The Thecate Marine Dinoflagellate Protoperidinium abei (Peridiniales, Dinophyceae) in Indonesia Danang Ambar Prabowo; Debora Christin Purbani; Diah Radini Noerdjito
HAYATI Journal of Biosciences Vol. 30 No. 2 (2023): March 2023
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.30.2.207-215

Abstract

Two identical dinoflagellate cell specimens were found in a seawater sample collected from Tanjung Pasir Beach (TPB; 6.01 S, 106.8 N), Banten, Indonesia and subjected to morphological characterization light microscopy (LM). The cells measured 71.97–72.86 µm long and 52.55–52.76 µm wide, fusiform or biconical-shaped, with tapered apical and antapical horns. A sharp and pointy spine was present at the antapex. The Kofoidean thecal plates arrangement pattern of the specimens was formulated as Po, 4', 2a, 7'', 3(?)c, 4s, 5''', 2''''. Based on this thecal plate tabulation formula combined with the cell morphologies, the specimens conformed to the original description of, and therefore identified as, Protoperidinium abei (Paulsen) Balech. This study constitutes the first record of P. abei in Indonesia and the first to illustrate and describe its detailed morphology using LM.
In Silico Analysis of Protein of Milk, Soybean, and Kefir as Anti- Thrombotic Bioactive Peptide Muhammad Ilham Fahri; Fadilla Sherlyna; Mochamad Nurcholis
HAYATI Journal of Biosciences Vol. 30 No. 2 (2023): March 2023
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.30.2.216-223

Abstract

Currently, Cardiovascular Diseases (CVDs) cases are increasing significantly in Indonesia and the world. WHO reported that in 2019-related CVD patients accounted for 85%, which increased to 87% in 2021. The cases increased significantly by 2%, which is a significant number as it covered the world population. Stroke is a disease affected by platelets undergoing thrombolysis by the α-thrombin (IIa) receptor with coagulation factors on the heart blood vessel. Chemical or synthesis drugs to treat thrombolytics such as heparin are already available, but they would be harmful if misused. In this study, we focused on the activity, interaction, and prediction of the physicochemical of anti-thrombotic peptides. We found that k-casein from kefir has the highest activity of 0.037 A based on BIOPEP analysis with 17 peptide libraries and molecular docking visualization analysis using PyMol, PyRx, and Discovery Studio for screening the best peptide. ToxinPred and AlgPred showed there are no potentially harmful peptides, but the protein variant of β-conglycinin subunit can be an allergen known to the immune system IgE. In conclusion, the GPR peptide has the highest binding affinity, with a total energy of -6.8 kcal/mol.
Effect of Different Enriched Crickets (Gryllus sigilatus) on Growth and Pigmentation of Asian Arowana (Scleropages formosus) Var. Super Red Sukarman Sukarman; Rendy Ginanjar; Mochammad Zamroni; Idil Ardi; Ahmad Musa; Melta Rini Fahmi; Agus Priyadi; Muhamad Yamin
HAYATI Journal of Biosciences Vol. 30 No. 2 (2023): March 2023
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.30.2.392-403

Abstract

This study aimed to evaluate the effect of crickets enriched with a source of carotenoids on fish’s growth, colour, and total carotenoid content in juvenile Asian Arowana (Scleropages formosus). Six juveniles were fed with different enriched crickets (G. sigilatus) based on the following treatments: (A) Crickets fed with fish feed without enrichment, (B) Crickets fed with fish feed containing 500 ppm of synthetic astaxanthin, and (C) Crickets fed with a combination of 50% fish feed, 48% sand crab meal and 2% marigold petal meal. The results of the study revealed that The weight gain (WG), specific growth rate (SGR), feed efficiency (FE), length gain (LG), and survival rate (SR) for treatment A were 110 g, 1.16% head/day, 45.79%, 10.98 cm, and 100% respectively. There was also no significant difference (P>0.05) with the other treatments. However, treatment B had a positive effect on the redness (a*) of the Arowana tail with a value of 9.52, and treatment C increased the yellowness (b*) of the Arowana body with a value of 2.77 (P<0.05), which was different to the other treatments. There were no differences between any of the treatments concerning the effects on lightness (L*), chromaticity (C*), and hue (H*) on either the body or the tail. The astaxanthin enrichment produced the highest carotenoid content (P<0.05) with a value of 45.09 µg/g. Enriching crickets with astaxanthin influence colour and carotenoid content but not the growth of the Asian Arowana fish.
Metabolites Profiling of Penicillium citrinum Recovered from Endophytic of Ramie (Boehmeria nivea) as a Potential Biocontrol Against Pathogenic Fungi Kartika Sari; Vira Kusuma Dewi; Asri Peni Wulandari; Nia Rossiana; Tati Herlina; Fitri Widiantini
HAYATI Journal of Biosciences Vol. 30 No. 2 (2023): March 2023
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.30.2.246-255

Abstract

Endophytes are a potent source of bioactive compounds that mimic plant-based metabolites. Fungi Fusarium spp. and Clonostachys rosea have been identified as pathogenic microorganisms in ramie (Boehmeria nivea). The antifungal test was carried out using the diffusion method and the MIC50 and Minimum Fungicidal Concentration (MFC) values using the tested microbial pathogens were Fusarium solani isolate 3248941, F. solani isolate Colpat-359, F. oxysporum isolate N-61-2, dan Clonostachys rosea strain B3042. Identification of secondary metabolites of the extract was carried out using GC-MS. The chromatogram of GC-MS analysis of this ethyl acetate extract (EA) showed seven dominant chemical compounds with various biological activities. Ethyl asetate extract of P. citrinum showed inhibition zone ranged from 19.10–22.07 mm with strong-very strong category at 200 mg ml-1 concentration against the tested microbial pathogens. Results revealed that the significant MIC values were observed against F. solani isolate 3248941 and Clonostachys rosea strain B3042 by less than 6.3  mg ml-1 and against F. solani isolate Colpat-359 and F. oxysporum isolate N-61-2 with 12.5 mg ml-1 and 25 mg ml-1 respectively. Minimum Fungicidal concentrations (MFC) for EA of P. citrinum were also reported against F. solani isolate 3248941 and C. rosea strain B3042, by 12.5 mg ml-1, whilst Fusarium solani isolate Colpat-359 by 25 mg ml-1 and Fusarium oxysporum isolate N-61-2 ,by 50 mg ml-1 respectively. Results of this study showed that P. citrinum, endophytic fungi of ramie, could be a promising source of compounds for antifungal agents.
Paederia foetida Linn Leaves-Derived Extract Showed Antioxidant and Cytotoxic Properties Against Breast Carcinoma Cell Jepri Agung Priyanto; Muhammad Eka Prastya; Gian Primahana; Ahmad Randy; Diyah Tri Utami
HAYATI Journal of Biosciences Vol. 30 No. 2 (2023): March 2023
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.30.2.271-280

Abstract

Paederia foetida Linn. is a rich source of active compounds, yet its antioxidant and cytotoxic properties remain poorly studied. This current research aimed to investigate the chemical profiles of a crude extract derived from P. foetida leaves, its fractionated substances, and their antioxidant and cytotoxic properties on MCF-7 cells, a breast carcinoma cell. Six fractionated substances have been separated in thin layer chromatography (TLC) plate. Five fractions showed antioxidant properties, as indicated by the formation of the yellowish band on the TLC plate after spraying with the DPPH reagent. Furthermore, crude extract of P. foetida leaves and its fractions are also characterized by antioxidant properties against 2, 2'-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radicals. F1 fraction exhibited the most potent antioxidant activity with IC50 values of 10.94±2.67 and 50.04±0.48 µg/ml, respectively, toward DPPH and ABTS radicals. Crude extract and all six fractions also varied in their cytotoxic properties against MCF-7 cells, with IC50 values ranging from 410.24±0.30 to 831.57±6.91 µg/ml. F3 fractions showed the strongest cytotoxicity (IC50 value: 410.24±0.30 µg/ml). Methanolic extract and active plant leaf fractions contained flavonoid and phenolic compounds, as measured by aluminum chloride and Folin-Ciocalteu reagent, respectively. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis showed that phenol, flavonoid, alkaloid, limonoid, and steroid were the major compound found in the crude extract and fractions. In conclusion, the present study proved that crude extract of P. foetida leaves and its fractionated substances were potential as antioxidant and cytotoxic agents.
Biodefluorination of Perfluorooctanesulphonate by Ensifer adhaerens M1 Sharipov Danil; Starikov Sergey; Chetverikov Sergey
HAYATI Journal of Biosciences Vol. 30 No. 2 (2023): March 2023
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.30.2.313-320

Abstract

A strain of bacteria M1, defluorinating perfluorooctanesulfonic acid (PFOS), was isolated from soil taken from the territory for testing fire extinguishing agents. The analysis of cultural, morphological, physiological and biochemical characters, the 16S rRNA gene sequencing allowed us to identify strain M1 as Ensifer adhaerens. The uniquely skill of the isolated strain to utilize PFOS in a mineral liquid medium for 5 days of cultivation was shown. It was found that E. adhaerens M1 transforms perfluorooctanesulfonic acid into perfluoroheptanoic acid, releasing 152 mg/L of free fluoride ions from 1,000 mg/L of PFOS. The data obtained allow us to recommend E. adhaerens M1 as potential treatment agent of the environment from organofluorine compounds.
Leclercia adecarboxylata C12, The Newly Isolated Cellulose-degrading Bacteria from Indonesian Coffee Pulp Agustin Krisna Wardani; Ajeng Astrini Brahmanti; Erryana Martati
HAYATI Journal of Biosciences Vol. 30 No. 3 (2023): May 2023
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.30.3.588-595

Abstract

Culturable cellulose-degrading microorganisms were collected from Arabica coffee pulp in East Java, Indonesia. Fifty isolates were obtained, and thirty-three isolates showed hydrolyzing zone on Carboxy Methyl Cellulose agar plates after Congo-Red staining. The highest specific CMCase activity was observed by isolates C12, identified as Leclercia adecarboxylata based on 16S-rRNA gene sequence analysis. SDS-PAGE of Leclercia adecarboxylata C12 cellulase revealed two bands with a molecular mass of 95.49 and 81.28 kDa, respectively. Activity gel analysis showed the cellulolytic ability of Leclercia adecarboxylata C12 cellulase by clear zone formation. The optimal CMCase activity was achieved at 50°C and pH 9, and the activity retained 47% of its initial activity after incubation at 50°C for 90 minutes. The purified enzyme remains stable from pH 5 to 10, with 77% of its maximum activity. The activity of CMCase was stimulated by the presence of K+, Ca2+, Mg2+, and Fe3+, while SDS and EDTA reduced its activity. The current study shows that the thermostable-alkalophilic cellulase produced by Leclercia adecarboxylata C12 is very promising for industrial applications.
Phytotoxic and Cytotoxic Polyketides Produced by Fungal Endophytes Isolated from Psidium guajava Tendy Oktriawan; Nanang Ariefta; Tri Joko Raharjo; Endang Astuti; Takuya Koseki; Yoshihito Shiono
HAYATI Journal of Biosciences Vol. 30 No. 3 (2023): May 2023
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.30.3.473-479

Abstract

Endophytic fungi reside within their host plants with no obvious symptoms. They have been proven as abundant sources of new bioactive compounds with diverse structures. In our continuous search of the new compound from endophytic fungi, a new compound, 5,7-dihydroxy-3(R)-methylphthalide (1), was isolated from Xylaria brevipes PGR1, and three known compounds, (–)-altenuene (2), alternariol (3), and altertoxin I (4), were isolated from Alternaria alternata D-8. Both endophytic fungi were isolated from the branch of an Indonesian medicinal plant, Psidium guajava. Their structures were elucidated using extensive spectroscopy analyses, including 1D/2D NMR, HRESITOFMS, and data comparisons with the literature. Compounds 1–3 exhibited phytotoxicity at IC50 (µg/ml) values of 4.6, 23.07, and 77.28, respectively. Additionally, 1–4 showed significant cytotoxicity in the brine shrimp lethality test (BSLT), with mortality rates at 75%, 50%, 90%, and 80% at the concentration of 0.19 μg/ml, respectively.
Enhancing Solubility of Recombinant Plasmodium Lactate Dehydrogenase (pLDH) Using Combination of Cold-Inducible Expression System and Cold-Stirred Bioreactor Alimuddin; Muhamad Ali; Sahrul Alim; Muhamad Amin
HAYATI Journal of Biosciences Vol. 30 No. 3 (2023): May 2023
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.30.3.561-566

Abstract

A major drawback associated with an expression of a high-level Plasmodium Lactate Dehydrogenase (pLDH) using Escherichia coli is the low solubility due to the formation of an inclusion body (IB). This study aimed to develop a suitable protocol for enhancing the solubility of pLDH expressed in E. coli. Firstly, a pLDH-encoding gene was amplified from the blood sample of malaria-infected patients and ligated into pBlueScript II KS+ for sequencing. Afterward, the pLDH gene was digested and cloned into pColdTF for expression. The recombinant plasmid was transformed into the E. coli BL21 (DE3) RIPL Codon Plus Strain. Then, the bacterial host was initially cultured at 37°C until reaching optical density (OD) at 600 nm: 0.5. Thereafter, the growth temperature was lowered to 15°C, followed by the addition of 0.1 mM IPTG into the culture medium for inducing pLDH expression. Thereafter, the bacterial hosts were cultured in a cold-stirred bioreactor (15°C). The result showed that a combination of the low culture conditions (15°C) and a low amount of IPTG increased the solubility of pLDH. This result suggests that this protocol can be a convenient method for generating high-quality recombinant protein using the E. coli system.
Expression of SARS-CoV-2 Nucleocapsid (N) Recombinant Protein Using Escherichia coli System Rizki Aulia Ansari; Uus Saepuloh; Silmi Mariya; Yuliana; Rachmitasari Noviana; Irma Herawati Suparto; Huda Shalahudin Darusman
HAYATI Journal of Biosciences Vol. 30 No. 3 (2023): May 2023
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.30.3.445-450

Abstract

One of the main antigen that can be used for serological testing is the nucleocapsid (N) which is the most abundant viral-derived protein in SARS-CoV-2 where this virus can cause COVID19 disease. The aim of this study was to develop the SARS-CoV-2 N recombinant protein using Escherichia coli expression system. A total of 1,089 nucleotides encoding 362 amino acids of SARS-CoV-2 N was cloned to pET-14b vector. The plasmid then expressed in E. coli BL21 (DE3) and induced with 1.0 mM IPTG (Isopropyl-β-d-1-thiogalactopyranoside). The cell was harvested using denaturation lysis buffer due to inclusion body formation of SARS-CoV-2 N protein. Dialysis processed and concentrated using PEG-6000 resulted 0.992 mg/ml protein yield. Analysis of SARS-CoV-2 N recombinant protein using SDS-PAGE technique showed approximately 37.0 kDa specific band target protein. Application of this SARS-CoV-2 N recombinant protein to vaccinated and non-vaccinated antibody serum samples using ELISA technique indicated the significant result of optical density mean at 0.603 and 0.135, respectively. This study revealed that the production of SARS-COV-2 N recombinant protein could be carried out in E. coli expression system under denatured conditions, therefore the methods are more effective in producing the protein as a basic material in immuno-diagnostic assay.

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