cover
Article Per Year (5 Year)
All
Home Page
OAI Link
Editorial Team
Contact
Reviewer
Google Scholar
Contact Name
-
Contact Email
-
Phone
-
Journal Mail Official
hayati_j_biosci@cbn.net.id
Editorial Address
-
Location
Kota bogor,
Jawa barat
INDONESIA
HAYATI Journal of Biosciences
Published by Institut Pertanian Bogor
ISSN : 19783019     EISSN : 20864094     DOI : -
Core Subject : Science, Agriculture,
Agriculture, Biological Sciences & Forestry Earth & Planetary Sciences
HAYATI Journal of Biosciences (HAYATI J Biosci) publishes articles and short communication in tropical bioscience fields such as development, biotechnology, biodiversity and environmental issues. HAYATI J Biosci covers wide range of all life forms topics including virus, microbes, fungi, plants, animal and human. HAYATI J Biosci has been also indexed/registered in Crossref, DOAJ, CABI, EBSCO, Agricola and ProQuest.
Arjuna Subject : -
Articles 1,091 Documents
 72   73   74   75   76 
Detection of Avirulence Gene AvrPi9 in Magnaporthe oryzae, a Rice Blast Fungus, Using a Combination of RPA and CRISPR-Cas12a Techniques Piyawan Puanprapai; Pattavipha Songkumarn; Theerayut Toojinda; Chatchawan Jantasuriyarat
HAYATI Journal of Biosciences Vol. 30 No. 5 (2023): September 2023
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.30.5.885-894

Abstract

Rice blast disease is one of the most devastating diseases of rice production worldwide, which causes by an ascomycete fungus, Magnaporthe oryzae. The virulence of the rice blast fungus is determined by avirulence genes (Avr genes). Therefore, the identification of Avr genes is important for rice resistance variety improvement. Avr genes are currently identified using the pathogenicity assay with rice near-isogenic lines (NILs) or PCR amplification and gene sequencing, both of which are time-consuming and labor-intensive methods. This study aims to develop a simple method for Avr gene identification using AvrPi9 as a model. A recombinase polymerase amplification (RPA) technique was carried out to amplify AvrPi9 by incubating rice blast fungus genomic DNA with gene-specific primers at 37°C for 20 min. Cas12a-based AvrPi9 detection was performed by incubating at 37°C for 5 min. The fluorescence signal was visualized by the naked eye under an LED transilluminator. The study found that AvrPi9 can be amplified and detected using RPA and a Cas12a-based method. AvrPi9_crRNA2 has a higher efficiency than AvrPi9_crRNA1. The sensitivity of the method was 3.8 ng of DNA target for AvrPi9_crRNA1 and 1.9 ng of DNA target for AvrPi9_crRNA2. This RPA and Cas12a combination technique is a newer method for Avr gene detection in plants and has several advantages over traditional methods. It is considered easier to use and more efficient in terms of time and labor, making it a potentially useful tool for plant breeders and pathologists.
Antioxidant Properties of Active Fraction Extract Derived from Yellow-Red Pigment Produced by the Marine Sponge-Associated Bacterium Bacillus haikouensis AGS112 and Identification of Related Compounds Uci Cahlia; Rika Indri Astuti; Jun Nomura; Aris Tri Wahyudi
HAYATI Journal of Biosciences Vol. 30 No. 5 (2023): September 2023
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.30.5.874-884

Abstract

Sponge-associated bacteria can produce bioactive compounds similar to the host. Here, the investigation of antioxidant properties of the yellow-red pigment produced by sponge-associated bacterium AGS112 was conducted using in vitro and in vivo analysis. The 16S rRNA gene sequence showed the sponge-associated bacterium AGS112 has the closest similarity with Bacillus haikouensis C-89 (99%). The crude pigment extract produced by Bacillus haikouensis AGS112 contained both phenolic and flavonoid. The peaks occurred at 412 nm and 664 nm, which indicated as the presence of carotenoids and flavonoids, respectively. The active fraction extract obtained through bio-autography TLC, and had more potent antioxidant activity against DPPH (2,2-diphenyl-1-picrylhydrazyl) compared to the crude pigment extract with an IC50 value of 68.62±0.59 µg/ml and 198.88±1.66 µg/ml, respectively. Moreover, the active fraction extract at the concentration of 35 µg/ml could better enhance the viability of Schizosaccharomyces pombe and achieve the highest expression of sod1 and ctt1 genes. Metabolite profiling using LC-MS analysis confirmed the active fraction extract contains eudesmin and artelastin that might be contributed as antioxidants. These results suggest that the active fraction extract of the yellow-red pigment produced by B. haikouensis AGS112 had potency to be used as candidate for natural antioxidants.
The Potential of Porang (Amorphophallus muelleri Blume) Flour and Porang Flour Formulation as an Anti-Diabetes Type-2 Agent Simon Bambang Widjanarko; Siti Nur Aisyiah Jamil; Eva Ni'maturohmah; Widya Dwi Rukmi Putri
HAYATI Journal of Biosciences Vol. 30 No. 5 (2023): September 2023
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.30.5.855-863

Abstract

Porang (Amorphophallus muelleri Blume) is a source of glucomannan in Indonesia, and a potential agent for diabetes mellitus treatment, apart from konjac. This study aims to determine the potential of porang flour formulation as a therapeutic agent for diabetes mellitus, especially type-2 diabetes mellitus. Soluble fiber, water absorption capacity(WAC), disintegration time, and viscosity of porang flour (PF) and porang flour formulation (PFF: consist of 85% porang flour, 1.03% k-carrageenan flour, 12% inulin flour, and 1.97% modified cassava flour (MOCAF)) were scrutinized. Twenty-eight male albino Wistar rats were randomly split into seven experimental groups. Five groups consisting of diabetic rats were treated using porang flour (300 mg/kg bw); low, middle, and high doses of porang flour formulation (100, 300, 500 mg/kg bw, respectively); and metformin (51.38 mg/kg bw). The rest were normal, and the diabetic (DM) control group. PF, PFF, and metformin were orally administered to the streptozotocin-induced diabetic rats per day for four weeks of the experiment. Fasting plasma glucose (FPG), Malondialdehyde (MDA) level, lipid profile, aspartate aminotransferase (AST), and alanine transaminase (ALT) levels of the blood plasma were measured, while the pancreas was used for immunohistochemical study and β-cells quantification. ANOVA was employed to analyze the data, followed by Honestly Significance Difference using Minitab version 17.0. The result indicated a significant effect of PF, PFF, and metformin on decreasing FPG and MDA and increasing the number of pancreatic β- cells in DM rats. Porang flour (300 mg/kg bw) and middle-dose PFF are potential therapeutic agents for type-2 DM.
Development of DNA Extraction Method for Forensics Studies of Preserved Hair and Skin Samples from Sumatran Tiger (Panthera tigris sumatrae, Pocock 1929) Noviar Andayani; Andi Eko Maryanto; Muhammad Naufal Nur
HAYATI Journal of Biosciences Vol. 30 No. 5 (2023): September 2023
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.30.5.816-824

Abstract

Poaching and illegal wildlife trade present severe threats to the Sumatran tiger. The high demand for tiger body parts leads to a high number of imitations in illegal markets, complicating the morphological identification of any confiscation cases. Accurate identification is essential in legal due process, given that the national protection law only regulates Indonesia's native species. Identification using molecular approaches may overcome the problem. However, most illegally traded tiger body parts have been preserved for an extended period of time, reducing the quantity and quality of the recovered DNA. This study aimed to develop a fast and effective method to recover DNA from preserved forensic samples. The methods had been tested with several museum samples of arsenic-treated hairs and a tiger skin piece obtained from the National Research and Innovation Agency (BRIN, formerly LIPI), tiger hairs obtained from Conservation of Natural Resources Agency (BKSDA) of Bengkulu Province, and a confiscated tiger skin sample from BKSDA Aceh. The DNA was extracted using ion-exchange, salting out, and protease-based methods. The results showed that the protease-based extraction outperformed the others to yield applicable DNA isolates for PCR-based species identification by Cyt b and ND2 mtDNA partial genes from preserved samples. However, further works are still needed to recover sufficient DNA yields for sex identification.
The Effect of Different Hydroponic Types and Nutrient Concentrations on the Chemical Composition and Antioxidant Capacity of Purwoceng (Pimpinella pruatjan) Extract Irmanida Batubara; Shadila Fira Asoka; Eni Sumarni; Herry Suhardiyanto; Mohamad Solahudin; Slamet Widodo; Supriyanto; Eti Rohaeti; Yudiwanti; Folkes Laumal; Erniati
HAYATI Journal of Biosciences Vol. 30 No. 5 (2023): September 2023
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.30.5.985-994

Abstract

Purwoceng (Pimpinella pruatjan) is an Indonesian native plant but difficult to cultivate. Hydroponics can be used as a solution for purwoceng cultivation. Hydroponic types and nutrient concentrations can affect purwoceng’s productivity. This study aimed to determine total phenolic content (TPC) and total flavonoid content (TFC), changes in steroid, and antioxidant capacity due to two hydroponic types (nonrecirculating and recirculating drip) and three nutrient concentrations (1,000, 1,500, and 2,000 ppm). Purwoceng was determined for its moisture content, ash content, and dry weight, and extracted with ethyl acetate for gas chromatography-mass spectrometry analysis and with ethanol for TPC, TFC, and antioxidant capacity. The data were analyzed statistically and grouped using principal component analysis (PCA) and heat map analysis (HMA). Nonrecirculating drip with high nutrient concentration at the aerial part was the best treatment to produce high TPC, TFC, and antioxidant capacity. There were no significant changes in steroid content. PCA showed that purwoceng extracts could be grouped in to two groups, i.e. aerial and root groups. HMA showed that purwoceng extracts could be distinguished from the abundance of palmitic acid and stigmasterol. The antioxidant capacity of purwoceng was directly proportional to the TPC value and also related to the presence of phytol, stigmasterol, and palmitic acid.
Comparative Morphology, Photoperiodism, and Yield of KDML105 Rice (Oryza sativa) and Its Mutants Ida Bagus Made Artadana; Kanyaratt Supaibulwatana
HAYATI Journal of Biosciences Vol. 30 No. 5 (2023): September 2023
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.30.5.937-945

Abstract

Climate change and decreasing in farmlands threaten global rice production. The productivity and grain quality of KDML105 (Oryza sativa ‘KDML105’), one of Thailand’s famous scented rice is depending on photoperiod and cultivating conditions. Cultivar improvement using induced mutagenesis has previously been performed and screening of candidate mutant lines (MT1, MT3, MT4, MT5, MT6) were then investigated in comparison with KDML105 to gain M6 generation. Comparative morphological features revealed that five mutant lines showed semi-dwarf shoot with erected leaves, higher tiller numbers, and compact clump when compared to KDML105. Interestingly, the variations of photoperiodism of flowering were observed among mutants and its original. All five lines of candidate mutants showed flowering unaffected by day length even under long-day or short-day that required only 47-85 days after transplanting to reach the flowering date. In contrast, KDML 105 showed flowering only under short-day conditions. On the other hand, three candidate mutants (MT3, MT5 and MT6) after screening for salinity tolerance were further selected to grow for M6 seed production. The tiller numbers, spikelet numbers, total grain yield, and grain weight obtained from MT3, MT5 and MT6 mutants were significantly more than those obtained from KDML 105. The mutants obtained in this study are characterized as intermediate photoperiodism with shortened production time. It is also suggested to further investigate the grain quality to examine the overall properties of these mutants prior to use in the rice breeding program.
Isolation and Selection of Antagonistic Bacteria against Cercospora arachidicola Causing Brown Spot on Peanut Khang Le Thanh; Thao Tran Yen
HAYATI Journal of Biosciences Vol. 30 No. 5 (2023): September 2023
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.30.5.927-936

Abstract

This study was conducted with the aim to isolation bacterial strains capable of antagonism to Cercospora arachidicola (Passalora arachidicola) causing brown spot in peanut. From 3 soil samples collected in the rhizosphere of peanut grown in Tay Ninh province, Vietnam, 10 bacterial strains were found having antagonistic action with Cercospora arachidicola. After 5 days of testing, the antagonistic efficiency of all isolates ranged from 33.33±1.28 to 60.183±0.927%. Studying the antagonistic mechanism showed that 5/10 isolates produced siderophore, 5/10 isolates were capable of decomposing chitin, 7/10 isolates capable of decomposing cellulase and 8/10 isolates proteolytic. Basing on the results of 16S rRNA gene sequence combined with morphological and biochemical characteristics, TN-TB 4, TN-TB 6 and TN-TB 12 were identified as Bacillus, representing the genera Bacillus amyloliquefaciens, Bacillus pasterurii, and Bacillus velezensis, respectively. These strains of antagonistic bacteria have the potential to produce probiotics.
α-Mangostin Effectively Inhibits Chikungunya Virus Replication in HepG2 Cells Moudy Soraya; Justus Sievers; Dionisius Denis; Anom Bowolaksono; R. Tedjo Sasmono
HAYATI Journal of Biosciences Vol. 30 No. 6 (2023): November 2023
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.30.6.1187-1193

Abstract

Chikungunya virus (CHIKV) is an arthropod-transmitted Alphavirus endemic to countries in Africa and Asia, including Indonesia, which causes debilitating arthralgia which can last several years. The rapid spread of CHIKV to new areas makes the discovery of antiviral agents a high priority. α-mangostin is a xanthone from mangosteen (Garcinia mangostana) pericarp and has antiviral activity against Hepatitis C and Dengue viruses. We investigated the antiviral activity of α-mangostin against CHIKV in HepG2 cells in pre-, post- and combination treatments compared to the common antiviral medicine ribavirin, as well their cytotoxicity. Our results show dose-responsive reductions in viral titer in all treatment regimes, with post- and combination treatments being more effective than pre-treatment only (IC50 = 7.79, 5.99 and 6.39 µM, respectively), but with poor specificity (SI = 1.39, 1.81 and 1.70, respectively) compared to ribavirin. Neither compound showed a direct virucidal effect. These results suggest α-mangostin effectively inhibits CHIKV replication in this cell line.
Diversity of Fungal Colonization in Respiratory Tract of Naïve Lung Cancer and The Emergence of Voriconazole Resistant Aspergillus Jamal Zaini; Abul A'la Al Maududi; Zahrah Annisa; Denny Grecius Siregar; Findra Setianingrum; Mulyati Tugiran; Ridhawati Sjam; Robiatul Adawiyah; Anna Rozaliyani; Sita Andarini; Elisna Syahruddin
HAYATI Journal of Biosciences Vol. 30 No. 6 (2023): November 2023
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.30.6.1139-1148

Abstract

Fungal spores in the air can be inhaled and enter the human respiratory tract. The entry of fungi into the respiratory tract can cause colonization or infection depending on the host immune response. Fungal colonization is the first step into debilitating fungal disease in humans, especially in immunocompromised groups. The increased rate of drug-resistant fungi has been reported in human disease and the environment. This study aims to examine the diversity of fungal colonization in humans and the rate of fungal resistance to voriconazole. This cross-sectional study was done in patients with naïve lung cancer who had not been previously treated with any cancer therapy nor given antifungal agent. Induced sputum from 70 subjects was collected and inoculated in the Sabouraud Dextrose Agar medium. Macroscopic and microscopic examinations were performed to identify fungal species. Voriconazole susceptibility tests were done using the disc diffusion method. This study found Candida albicans, Aspergillus niger, Aspergillus fumigatus, and Penicillium sp. among the most common lower respiratory tract colonies. This study also found the colonization of up to 5 species in a single subject. A high rate of voriconazole-resistant Aspergillus sp. was found (42.4%) among 59 isolates tested. Given that these subjects had never taken antifungal agents previously, the high rate of voriconazole resistance might be attributed to the environment, such as community and agriculture. Mitigation of antifungal use in the agricultural sector, fungal diversity in the environment, and clinical study of fungal colonization/ infection in other high-risk groups are needed.
Assessment of Teratogenic Effects of Sappan Wood (Caesalpinia Sappan L.) Extract In Rats (Rattus novergicus) Jeri Nobia Purnama; Erick Khristian; Mas Rizky A.A Syamsunarno; Yusof Kamisah; Ratu Safitri
HAYATI Journal of Biosciences Vol. 30 No. 6 (2023): November 2023
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.30.6.1036-1042

Abstract

Treatment using plant materials has been widely researched and observed to assist in curing a disease. Medicinal products derived from herbal plants are proven useful as drugs must follow guidelines not to cause acute or chronic toxicity. The purpose of this study was to investigate the teratogenic potential of an ethanolic sappan wood extract on the growth of unborn rats. Six groups of pregnant rats are created: Aquadest was administered to the Control group, whereas the Treatment groups received 100, 200, 300, 400, or 500 mg.kg-1 BW of an ethanolic extract of Sappan wood. On the twenty-first day of gestation, pregnant rats were CO2-euthanized and delivered vaginally. Rats' body weight during pregnancy was observed, along with fetal growth measurements, viscoelastic examination, and skeletal development. Wistar rats' reproductive systems, fetal body weight, body length, and tail length were all unaffected by ethanol extract of Sappan wood at doses from 100 mg/kg to 500 mg/kg. In fetuse rat from the control group and the five dosage groups, no skeletal development had taken place and there were no obvious external abnormalities. Even at the maximum dose of 500 mg, sappan wood extract demonstrated no teratogenic effects on the development of fetal rats.kg-1 BW.

Page 74 of 110 | Total Record : 1091

 72   73   74   75   76 

Filter by Year

2005 2026


Reset
Filter By Issues
All Issue Vol. 33 No. 3 (2026): May 2026 Vol. 33 No. 2 (2026): March 2026 Vol. 33 No. 1 (2026): January 2026 Vol. 32 No. 6 (2025): November 2025 Vol. 32 No. 5 (2025): September 2025 Vol. 32 No. 4 (2025): July 2025 Vol. 32 No. 3 (2025): May 2025 Vol. 32 No. 2 (2025): March 2025 Vol. 32 No. 1 (2025): January 2025 Vol. 31 No. 6 (2024): November 2024 Vol. 31 No. 5 (2024): September 2024 Vol. 31 No. 4 (2024): July 2024 Vol. 31 No. 3 (2024): May 2024 Vol. 31 No. 2 (2024): March 2024 Vol. 31 No. 1 (2024): January 2024 Vol. 30 No. 6 (2023): November 2023 Vol. 30 No. 5 (2023): September 2023 Vol. 30 No. 4 (2023): July 2023 Vol. 30 No. 3 (2023): May 2023 Vol. 30 No. 2 (2023): March 2023 Vol. 30 No. 1 (2023): January 2023 Vol. 29 No. 6 (2022): November 2022 Vol. 29 No. 5 (2022): September 2022 Vol. 29 No. 4 (2022): July 2022 Vol. 29 No. 3 (2022): May 2022 Vol. 29 No. 2 (2022): March 2022 Vol. 29 No. 1 (2022): January 2022 Vol. 28 No. 4 (2021): October 2021 Vol. 28 No. 3 (2021): July 2021 Vol. 28 No. 2 (2021): April 2021 Vol. 28 No. 1 (2021): January 2021 Vol. 27 No. 4 (2020): October 2020 Vol. 27 No. 3 (2020): July 2020 Vol. 27 No. 2 (2020): April 2020 Vol. 27 No. 1 (2020): January 2020 Vol. 26 No. 4 (2019): October 2019 Vol. 26 No. 3 (2019): July 2019 Vol. 26 No. 2 (2019): April 2019 Vol. 26 No. 1 (2019): January 2019 Vol. 25 No. 4 (2018): October 2018 Vol. 25 No. 3 (2018): July 2018 Vol. 25 No. 2 (2018): April 2018 Vol. 25 No. 1 (2018): January 2018 Vol. 24 No. 4 (2017): October 2017 Vol. 24 No. 3 (2017): July 2017 Vol. 24 No. 2 (2017): April 2017 Vol. 24 No. 1 (2017): January 2017 Vol. 23 No. 4 (2016): October 2016 Vol. 23 No. 3 (2016): July 2016 Vol. 23 No. 2 (2016): April 2016 Vol. 23 No. 1 (2016): January 2016 Vol. 22 No. 4 (2015): October 2015 Vol. 22 No. 3 (2015): July 2015 Vol. 22 No. 2 (2015): April 2015 Vol. 22 No. 1 (2015): January 2015 Vol. 21 No. 4 (2014): December 2014 Vol. 21 No. 3 (2014): September 2014 Vol. 21 No. 2 (2014): June 2014 Vol. 21 No. 1 (2014): March 2014 Vol. 20 No. 4 (2013): December 2013 Vol. 20 No. 3 (2013): September 2013 Vol. 20 No. 2 (2013): June 2013 Vol. 20 No. 1 (2013): March 2013 Vol. 19 No. 4 (2012): December 2012 Vol. 19 No. 3 (2012): September 2012 Vol. 19 No. 2 (2012): June 2012 Vol. 19 No. 1 (2012): March 2012 Vol. 18 No. 4 (2011): December 2011 Vol. 18 No. 3 (2011): September 2011 Vol. 18 No. 2 (2011): June 2011 Vol. 18 No. 1 (2011): March 2011 Vol. 17 No. 4 (2010): December 2010 Vol. 17 No. 3 (2010): September 2010 Vol. 17 No. 2 (2010): June 2010 Vol. 17 No. 1 (2010): March 2010 Vol. 16 No. 4 (2009): December 2009 Vol. 16 No. 3 (2009): September 2009 Vol. 16 No. 2 (2009): June 2009 Vol. 16 No. 1 (2009): March 2009 Vol. 15 No. 4 (2008): December 2008 Vol. 15 No. 3 (2008): September 2008 Vol. 15 No. 2 (2008): June 2008 Vol. 15 No. 1 (2008): March 2008 Vol. 14 No. 4 (2007): December 2007 Vol. 14 No. 3 (2007): September 2007 Vol. 14 No. 2 (2007): June 2007 Vol. 14 No. 1 (2007): March 2007 Vol. 13 No. 4 (2006): December 2006 Vol. 13 No. 3 (2006): September 2006 Vol. 13 No. 2 (2006): June 2006 Vol. 13 No. 1 (2006): March 2006 Vol. 12 No. 4 (2005): December 2005 Vol. 12 No. 3 (2005): September 2005 Vol. 12 No. 2 (2005): June 2005 Vol. 12 No. 1 (2005): March 2005 More Issue