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INDONESIA
Indian Journal of Forensic Medicine & Toxicology
Published by Official Organ of Indian Association of Medico-Legal Expert
ISSN : -     EISSN : -     DOI : -
Core Subject : Health,
Public Health
Arjuna Subject : Kedokteran - Epidemilogi
Articles 5,497 Documents
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The Effect of Pollen Type on Some Fruit Traits and Production Quantity of Date Palm Trees Phoenix Dactylifera L. For Agricultural Cultivar Al-Ibrahimi Uday Hatem Khaeer Abed1 , Abed-Almajeed Abed-Alazeez2
Indian Journal of Forensic Medicine & Toxicology Vol. 14 No. 4 (2020): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v14i4.11763

Abstract

The study was conducted during the agricultural season 2019 in one of the orchards of the district of Heat,Al-Hassania (Shamiya) Village in Al-Anbar Governorate, know the effect of different pollen male grainstype (Ghanami Ahmar, Ghanamy akhdar, samesmi , Khikri adi ) on some fruits traits and the amount ofproduction the date palm of the agricultural cultivar AL-Ibrahimi, The results indicated that the best pollenused to pollinate the agricultural cultivar Ibrahimi it is for grains of the vaccine Khikri adi, characterizedby giving the best qualitative qualities of fruits Average weight fruits and diameter in the Khalal stageand average diameter and weight stromal layer in the stage dates (11.59 g , 2.36 cm , 1.77 cm , 9.96 g)respectively , the Ghanami Ahmar vaccine was distinguished by giving the best quantitative fruit qualitiesthe average of percentage for fruits contract and raceme weight (86.60 % , 16.87 Kg ) respectivel .
Isolation and Molecular Detection of Salmonella Infantis from Milk and Children with Gastroenteritis in Babylon Province, Iraq Niran Kadhim F. Al-Rubaey1 , Alyaa Mohammed H. Al-Tabtabai2 , Khaleid Yassen Al-Zamily3 ,
Indian Journal of Forensic Medicine & Toxicology Vol. 14 No. 4 (2020): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v14i4.11764

Abstract

Two hundred and ten samples were included in this study. The samples encompassing of 90 cattle milksamples obtained from local markets, and 120 stool samples obtained from children with gastroenteritisof age less than 12 years from both genders who were attending to the Babylon maternity and children’shospital. The samples were collected during the interval from February to August 2018 in Babylon province.All these 210 samples were subjected to many tests for isolation and identification of Salmonella species byusing bacterial culturing, biochemical tests, and API20E test system. The results showed that the prevalenceof identifying Salmonella isolates were 69 (32.9%) out of 210 samples, distributed as (25/90) (27.8%) marketmilk and (44/120) (36.7%) stool samples. The identified Salmonella isolates were subjected to serotyping bymonovalent antisera. It was found that 4 out of 25 isolates (16%) in market milk samples and 16 out of 44isolates (36.4%) in stool samples that belong to Salmonella infantis. PCR technique was achieved to identifyinvA and invF virulence genes in Salmonella infantis strains. All 20 isolates of Salmonella infantis in marketmilk and stool samples were examined. The results revealed that, invA gene was detected in all strains ofSalmonella infantis isolated from the market milk and stool samples, while the invF gene was present in2 strains of the market milk Salmonella infantis, but existing in all strains of the stool Salmonella infantis.
Evaluation Study of Different Techniques in Diagnosis of Acute Leukemia in Adults and Children Patients Rash Hamid Ayub1 , Ayad Hameed Ibraheem2, Najwan Salih Nima3
Indian Journal of Forensic Medicine & Toxicology Vol. 14 No. 4 (2020): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v14i4.11765

Abstract

Cytomorphology and cytochemistry are combined with immunophenotype by flow cytometry (FCM) todiagnosis and management the acute leukemia. The present study was designed to compare between thecytomorphology, cytochemistry and immunophenotype by flow cytometry at diagnosis and classification ofacute leukemia to suggest a number of antibodies that can be adopted in the diagnosis by flow cytometry.The study included (160) cases were newly diagnosed (100 adults, 60 children). Morphological analysiswas depended on peripheral blood smears examination by Leishman stain, cytochemical analysis with PASand SBB stains and immunophenotype analysis used multiparameter. the results of this study shown that60% of adult cases were AML, and 40% ALL. The mean age of adults was 48.45 years for AML cases andwas 32.59 years for ALL adult cases. In children AML the mean age was 5.23 years and was 6.44 years inchildren ALL cases. Our Conclusion Also that the B.ALL was more commonly than T.ALL in both adultsand children cases. Immunophenotypic detection by flow cytometry recorded (100%) successfully diagnosisin ALL cases, but it was successfully in (75%) of detected the subtypes of AML cases.
Analysis of Blood Spot in the Crime Scene in Relation to the Environmental Parameters Nesreen Muneam Yaseen1 , Ayad M. J. Al-Mamoori2
Indian Journal of Forensic Medicine & Toxicology Vol. 14 No. 4 (2020): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v14i4.11766

Abstract

This study was designed to evaluate the analysis of blood samples in crime scene under different environmentalconditions. Samples are divided into four main groups depending on the source of bloodspot: Soil, Stone,Ceramic, and Water sample. Each group consisted of two sub-groups depending on weather temperature inIraq in winter and summer: 8 °C groups and 45 °C groups. Each group consisted of 32 samples. Sampleswere collected after different time (0, 2, 6, and 24) hours.The results of the current study showed variation in the ability to extract DNA depending on the surface type.In addition, DNA can be extracted from bloodstains on soil, gravel, ceramics, and water when extractedimmediately after exposure to the surface. The difference in the ability to extract DNA from bloodstainson different surfaces after two hours at 8 °C temperature, where the success rate of extraction is (25, 7.5,100, and 100%) for soil, stone, ceramics, and water samples Respectively. The ability rate to extract DNAafter two hours at 45 °C temperature, where (25, 7.5, 87.5, and 100%) for soil, gravel, ceramics, and watersamples respectively. The ability rate to extract DNA after six hours at 8 °C temperature, where (0, 12.5,37.5, and 100%) for soil, gravel, ceramics, and water samples respectively, while it was (0, 12.5, 25, and100%) for six hours samples at 8 °C temperature. After 24 hours, only water samples showed the ability toextract DNA from all samples, while no DNA was obtained from the rest of the samples.PCR amplification of the FIF2AK3 gene showed products when the DNA extracted immediately after theiradhesion to the surface of the soil while showing no products for other age groups. Also, the PCR productscan be obtained when DNA isolate immediately after exposure to the stone surface, whereas the rate PCRamplification products of DNA isolated after two hours was (37.5%) for both groups 8 °C and 45 °C., whileother age groups showing not products.The agarose gel electrophoresis of PCR products showed the possibility of FIF2AK3 gene amplificationwhen blood samples were taken immediately after exposure to the surface, whereas the ability to obtainamplification products was (100 and 87.5%) for groups 8 °C and 45 °C respectively. No products wereobtained after 6 and 24 hours. In water samples, all isolated DNA gave the product.
Determination of IL17 Plasma Levels and IL-17A gene Polymorphisms in Patients with Allergic Rhinitis Mohammed D. D. AL-Beagy1 , Thanaa M. Jouda2 , Safaa H. Alturaihy3
Indian Journal of Forensic Medicine & Toxicology Vol. 14 No. 4 (2020): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v14i4.11767

Abstract

Aim: The present study aims to determine whether the level of serum interleukin -17 and IL-17 receptors (IL-17A) gene variant increases or decreases susceptibility to Allergic Rhinitis. Method: In this case-controlstudy fifty patients with rhinitis and fifty apparently healthy persons as a control in Babylon province /Iraq were enrolled in this study. Results: the results of this study were shown significant difference infrequency of IL-17 A (rs2275913) A>G polymorphism between Rhinitis patients and controls .Also , therewere significant difference in frequency of IL-17A (rs 2275913) between rhinitis patients and controls andthere were significant increase in patient compared with control. Conclusion: The bpresent study suggeststhat patients with AA allele for IL-17A (rs2275913) have increased susceptibility to rhinitis compared withGG allele , Also the present study suggests that IL-17A (rs2275913) is considered risk factor for AllergicRhinitis. This study suggests that the level of IL-17 patients with rhinitis highly significant compared withhealthy.
Activity of Extract Seed Moringa Oleifera on Gene Expression of (EFB1) Gene in Candida dubliniensis Isolated from oral Candidiasis to Cancer Patients Zahraa Khudhair Abbas Al-Khafaji1 Farqad Abdulelah Ali AL-Janahi2 , Al-karrar Kais abdul Jaleel Duai
Indian Journal of Forensic Medicine & Toxicology Vol. 14 No. 4 (2020): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v14i4.11768

Abstract

Gene expression of EFB1 gene in yeast Candida dubliniensis isolated from oral candidiasis in cancerpatients was detected using by R-time PCR for some isolates before and during the yeast infection of theepithelial cells. The results showed a high rate of gene expression of T2 ( 42.104 ± 6.533) and a significantdifference at the probability level P>0.01 compared with the treatment was c (1.034 ± 0.126 ) and the useof the Moringa oleifera extract was 25% to evaluate its effect on the gene expression (ALS1) before andduring yeast infection of epithelial cells. The results showed that the gene expression of T1 was decreasedby ( 0.129 ± 0.021) compared to (C) value (1.034 ± 0.126) .As for the T2 treatment was gene expression at a rate (42.104 ± 6.533) high Compared with T3 treatment inwhich gene expression dropped at a rate (22.143 ± 4.176 ) and with a significant difference at the level ofthe probability of P ? 0.01.
Reducing the Environmental Impact of Cork Waste by Improving Natural Asphalt as Water Proofing Material Bashar Abdulazeez Mahmood1, Majid Ahmed Mohaisen 2 , Adil Hasan Mahmood3 , Saadoon O. Eyada4
Indian Journal of Forensic Medicine & Toxicology Vol. 14 No. 4 (2020): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v14i4.11769

Abstract

This research is concern with two principles, a) the environmental pollution which is the global problem b)the economic benefits as a result of reusing of waste material such as corks to produce new reusable materiallike Modified-Asphalt. In this research cork has melted by benzene and mixed with Hit Natural Asphalt toget good paste with weight percentages of 0%, 10%, 20%. The properties of Modified –Asphalt have beenstudied after and before adding coke. The results revealed that adding coke by 29% can make the Modified–Asphalt meets the international specifications (ASTM D312) and Iraqi specifications (88-1196) as a Waterproofing material.
Assessment the Effect of Lactobacillus Acidophilus on Escherichia Coli Serotype O157:H7 with Detection of Some Virulence Factors Mokhtar Jawad AL-Imam1 , May Talib Flayyih2
Indian Journal of Forensic Medicine & Toxicology Vol. 14 No. 4 (2020): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v14i4.11770

Abstract

Aim: To evaluation the effect of Lactobacillus acidophilus on Enterohemorrhagic Escherichia coli (EHEC)serotype O157:H7 with detection of some virulence factors.Methods: Two hundred and fifty specimens (stool) from children under five years for both sexes werecollected from some hospitals. All isolates were diagnosed according to morphological characteristics,biochemical tests. Monoplex pattern of PCR was used also for detection different genes in (7) Escherichiacoli )O157:H7 (isolates; include 16SrRNA, eae, lifA, Stx1,Stx2 that encoded for ribosomal RNA, intimin,lymphocyte inhibitory factor, shiga toxins. Three types of probiotics strains were obtained, Lactobacillusfermentum, Lactobacillus plantarum and Lactobacillus acidophilus (ATCC4356). Minimum inhibitoryconcentration (MIC) of cell free supernatants of Lactobacillus acidophilus was determined by employingdifferent dilutions (1/2, 1/4, 1/8, 1/16, 1/32), to detect the concentration of probiotic that will inhibit E.coli(O157:H7) growth.Results: Results showed, 210 (84%) samples were identified as E.coli from 250 samples. The result showed(7) isolates were identified as Enterohemorrhagic Escherichia coli (EHEC) serotype O157:H7 and showed allisolates of O157:H7 were positive for 16SrRNA gene with (213bp) and eae with (741bp), lifA with (712bp),only Stx1 gene appeared in all isolates with (446bp) and no bands with Stx2. Current result showed only cellfree culture supernatant of Lactobacillus acidophilus has inhibitory activity against all E.coli (O157:H7)isolates with different dilutions (1/2, 1/4, 1/8, 1/16, 1/32), while Lactobacillus fermentum, Lactobacillusplantarum have no effect against E.coli (O157:H7). The result showed the bacteriocin has inhibitory effectagainst E.coli (O157:H7), while organic acids and hydrogen peroxide haven’t any role in inhibition. The(MIC) value was (1/8) which inhibits the bacterial growth of isolates.
Effect of Conocarpus Erectus Extraction on Some Physiological and Biochemical Parameters on Male Rats Adel M. Al Zobidy
Indian Journal of Forensic Medicine & Toxicology Vol. 14 No. 4 (2020): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v14i4.11771

Abstract

The present study was conducted to evaluated some of physiological and biochemical parameters ,we usedeighteen of males rats divided randomly to three group ( six in each ), group one considered as control,group two the animal treated with 250 mg /kg and group three treated with 500 mg /kg .The result shownon-significant appeared in RBC , Hb, PCV ,MCH , MCV and MCHC ,while the WBC , lymphocytes andgranular show increased significantly . The biochemical test show increased significantly in liver enzymeconcentration ALT ,AST and ALP also the result of lipid profile appeared decreased in al parametersexception the HDL which appeared increased significantly .
Synergistic Effect of Amikacin and Ciprofloxacin on pelA and algD Genes in Pseudomonas aeruginosa Wathiq Abbas Hattie Al-draghi1 , Rami Abd Al-Kareem Saeed2
Indian Journal of Forensic Medicine & Toxicology Vol. 14 No. 4 (2020): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v14i4.11772

Abstract

Pseudomonas aeruginosa is one of the most important pathogen in both hospital and community-acquiredinfections, it is a classical opportunistic pathogen, and this organism does not normally attack healthytissue, and rarely causes infection in the general population. It is possess various virulence mechanismsto adhere, colonize and biofilm formation. Samples were collected from different hospitals in Baghdad,samples were collected and categorized in to two main groups: eighty clinical samples and forty as anenvironmental samples. Forty one isolates were identified as P. aeruginosa by using conventional methodsand confirmed by VITEK-2 compact system, biofilm were detected by three methods: congo-red agar, tubemethod and microliter plate. Synergistic Effect of Amikacin and Ciprofloxacin on biofilm formation weredetected phenotypically by using microliter plate and genetically by Real-time PCR for pelA and algDGenes which are responsible for biofilm formation. The results shows an obvious differences in biofilmformation phenotypically and genotypically for the treated isolates

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