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Jurnal Fitopatologi Indonesia

jfiti
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Published by Institut Pertanian Bogor

ISSN : 02157950 EISSN : 23392479 DOI : -

Core Subject : Health,

Medicine & Pharmacology

Jurnal Fitopatologi Indonesia (JFI) is an official publication owned by the Indonesian Phytopathology Society (Perhimpunan Fitopatologi Indonesia). In 2010, JFI management was given to PFI Komda Bogor. Since then, JFI has been published 6 times (January, March, May, July, September, and November).

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Articles 412 Documents

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Meloidogyne incognita Penyebab Umbi Berbintil pada Kentang di Beberapa Sentra Produksi Kentang di Jawa Aprilyani Aprilyani; Supramana Supramana; Gede Suastika
Jurnal Fitopatologi Indonesia Vol 11 No 5 (2015)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Root knot nematodes is an important pathogen on potatoes in tropical and sub-tropical areas. Root knot nematodes contribute a significant impact in reducing the quality and quantity of potato tuber. Meloidogyne incognita is one of the species causing the root knot. This research was conducted to identify M. incognita on potatoes in Java Island based on morphological and DNA molecular characteristic. The infected potato tubers with pimple-like knot symptom were collected from Pangalengan (West Java), Banjarnegara (Central Java), and Kota Batu (East Java). Nematode was identified based on morphological character of perineal pattern, and based on molecular DNA character by polymerase chain reaction technique using a pair of specific primer (MI-F and MI-R), followed by DNA fragment sequencing and phylogenetic analysis. Based on morphological character of perineal pattern, M. incognita was detected in all 3 locations; while based on DNA molecular character, and M. incognita was detected in Pangalengan (West Java) and Kota Batu (East Java). M. incognita from Pangalengan had high homology, i.e.99.2% to 99.8% with those isolates from China, India, and Malaysia.

Ketahanan Lapangan Lima Genotipe Padi terhadap Penyakit Hawar Daun Bakteri Rezki Heru Aditya; Wiwiek Sri Wahyuni; Paniman Ashna Mihardjo
Jurnal Fitopatologi Indonesia Vol 11 No 5 (2015)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Bacterial leaf blight disease caused by Xanthomonas oryzae pv. oryzae is an important disease on rice in Indonesia. Four rice varieties, i.e. Inpari 30, Situbagendit, Luk-ulo and Cibogo has been known to have resistance to the disease. Therefore, they can be used as indicator plants to measure the resistance of any new rice genotypes to the disease. Research was aimed to evaluate field resistance of a new rice line, i.e. line X, with 4 resistant rice varieties as check control. The research was conducted in the field in Wirolegi villages, Sumbersari-Jember with natural infection of X. oryzae. Plant resistance was observed by measuring disease incidence (DI) and severity (DS). The highest DI (100%) was reached by all genotypes in different age. At 90 days after planting, DS of line X reached 11.85% which is far low compared to DS of var Situbagendit (40.25%). Based on DI and DS, line X is considered to have the best resistance to the disease, i.e. resistant in vegetative phase and moderately resistant in generative phase. However, the strain of X. oryzae infecting the plants in the field was unknown.

Risiko Introduksi Gandum ke Timor Tengah Utara: Penyakit Hawar Daun dan Busuk Batang Aloysius Rusae; Efi Toding Tondok; Suryo Wiyono
Jurnal Fitopatologi Indonesia Vol 11 No 5 (2015)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Wheat has a great potency to be cultivated in Timor Tengah Utara (TTU). However, disease is one of the risks for introduction of wheat. The existence of wheat disease in TTU is unknown because wheat has never been grown in this area. This study aims to determine the potential risks of major diseases that will infect wheat plants when introduced to the TTU. The methods used in this study consisted of wheat cultivation in the field, followed by observation and identification of the main diseases on wheat. Wheat varieties grown in field were Dewata, Selayar and Nias. Koch’s postulate were performed to identify the suspected microbes as pathogens. Morphological-based identification was applied on the isolated pathogens. The main diseases on wheat cultivated in TTU were leaf blight and stem rot. The results of Koch’s postulate showed that Helminthosporium gramineum was the causal of leaf blight and Rhizoctonia sp. was the causal of stem rot. Disease incidence reached 82–93% and 11–22% for leaf bligh and stem rot disease, respectively. Disease severity of leaf blight and stem rot disease on var. Dewata was the lowest compared to var. Selayar and var. Nias.

Laporan Pertama Infeksi Begomovirus pada Tanaman Mentimun di Bali I Dewa Made Putra Wiratama; Gusti Ngurah Alit Susanta Wirya; I Dewa Nyoman Nyana; Ni Nengah Putri Adnyani; Gede Suastika
Jurnal Fitopatologi Indonesia Vol 11 No 5 (2015)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Leaf yellowing symptoms was commonly found in cucumber plants in Bali provinces, i.e. in Apuan and Bangli villages recently. Begomovirus infection is suspected as the causal agent, due to similar symptoms previously reported from cucumber plants in Java. In addition, Bemisia tabaci was observed in the field. The objective of this research was to identify the causal agent of leaf yellowing disease of cucumber in Bali. Virus detection and identification was conducted by polymerase chain reaction method using universal primers for Begomovirus, i.e. SPG1/SPG2. DNA fragment of 912 bp in size was successfully amplified from leaf samples. Analysis of nucleotide sequencing indicated that Begomovirus infecting cucumber plants in Bali has the highest homology (91%) with Squash leaf curl China virus (SLCCNV) isolate from Malaysia. This is the first report of SLCCNV infection in Bali.

Pengaruh Ekstrak Daun Sirih dan Metode Ekstraksinya Dalam Menghambat Penyakit Antraknosa pada Cabai Pascapanen Desi Trisnawati; Lilik Pujantoro Eko Nugroho; Efi Toding Tondok
Jurnal Fitopatologi Indonesia Vol 15 No 6 (2019)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Extract of Piper betle as An Inhibitor of Anthracnose Postharvest Disease on Chili Pepper Chili is a horticultural commodity that mostly needed by consumers in the fresh condition. However, there is an interval of time needed to deliver it from producers (farmers) to consumers. During the supply chain, deterioration of the quality of chilli fruit can occur by the presence of anthracnose disease caused by Colletotrichum spp. The use of syntetic fungicides to control anthracnose disease is not recommended because it will leave residues that are harmful to consumers. The alternative that can be done is to use botanical pesticides as fungicides. The aim of this research is to study the potential of Piper betle leaves extract (PLE) in inhibiting the development of the antracnose disease caused by Colletotrichum acutatum on the chili during storage. The sequences of this research were strarted with production of PLE by boiling method which was preceded by blanching, chopping, and without treatment of betel leaves, then bioassay of betel extract by measuring the diameter of fungus growth on PDA+ PLE. The third test was application of PLE on fresh chili fruits by spraying and soaking, continued by observed the antracnoseinsidence on chili during storage time (21 days). The results showed that the chopping method was the best betel leaf extraction method among other treatments, the use of 10% PLE concentration was the most effective concentration in inhibiting the growth of C. acutatum in vitro, spraying treatment using 10% EDS was the most effective in inhibited the insidence of anthracnose disease.

Mikobiota pada Buah Cabai untuk Pengendalian Hayati Colletotrichum capsici Okky Setyawati Dharmaputra; Lisdar Idwan Sudirman; Melly Fitriani
Jurnal Fitopatologi Indonesia Vol 11 No 5 (2015)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Colletotrichum capsici is a pathogenic fungus causing anthracnose on various tropical fruits, especially chilli. Biological control agents have been used as an alternative method to control postharvest diseases. This study aims to examine the antagonistic potential of mycobiota on red chilli fruit against C. capsici. The pathogen was obtained from diseased red chilli fruits collected from three traditional markets in Municipality of Bogor, isolated on potato dextrose agar (PDA) medium containing chloramphenicol (100 mg L-1). Candidates of antagonistic mycobiota were isolated from healthy chilli fruits using serial diution method, followed by pour-plate method on PDA medium containing chloramphenicol (100 mg L-1). C. capsici isolate BIO51046 showed highest pathogenicity on chilli fruit var. IPB Perbani compared to other isolates. Seven isolates of filamentous fungi and 7 yeast isolates were obtained from healthy chilli fruits. Test of antagonism using dual culture method obtained 3 filamentous fungal isolates (Plectosphaerella cucumerina, MF2 and Aspergillus flavus) and 1 yeast isolate (Issatchenkia orientalis) which inhibited the growth of C. capsici BIO 51046 more than 70%. Plectosphaerella cucumerina and I.orientalis did not cause any diseases on chilli fruits var. IPB Perbani. Therefore, these 2 isolates were considered as potential antagonist against C. capsici BIO51046 as the causal agent of anthracnose of chilli

Bakteri Endofit dari Tanaman Kehutanan sebagai Pemacu Pertumbuhan Tanaman Tomat dan Agens Pengendali Meloidogyne sp. Abdul Munif; Arif Rafi Wibowo; Elis Nina Herliyana
Jurnal Fitopatologi Indonesia Vol 11 No 6 (2015)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Meloidogyne sp. is one of the main constraints of tomato production in Indonesia. Endophytic bacteria may be considered as biocontrol agents for controlling Meloidogyne sp. The objective of this study was to isolate endophytic bacteria from forestry plants and to evaluate its potential for controling Meloidogyne sp. on tomato. Endophytic bacteria were isolated from roots of mahoni (Swietenia mahogany), trambesi (Albizia saman), gaharu (Aquilaria malacensis), and meranti (Shorea sp.). Isolation of bacterial endophytes from plant tissue was conducted using surface sterilization method with 70% alcohol, 3% NaOCl and sterile water on medium trypsic soy agar. Endophytic bacteria was separated and purified based on shape and color of the colony. A total of 33 isolates of endophytic bacteria were isolated from roots of mahoni (11 isolates), trambesi (5 isolates), gaharu (7 isolates), and meranti (10 isolates). The bacteria was tested for the hypersensitivity reaction on tobacco plants and the result showed that 22 isolates did not cause necrosis, indicated they are not pathogenic. Ten isolates of endophytic bacteria was selected for further experiment, i.e. to evaluate their potential as biocontrol agent for Meloidogyne sp. and as growth promotor for tomato plants. The experiment was conducted in the screenhouse using seed treatment. The result showed that two isolates of endophytic bacteria, i.e. MSJ1H and AGS1F were able to increase the growth of tomato plants up to 60% and reduce the number of root knot caused by Meloidogyne sp. Endophytic bacteria isolated from forestry plants have the potential as a biocontrol agents to plant parasitic nematode Meloidogyne sp.

Identifikasi Gen elF4G asal Oryza rufipogon pada Padi Varietas Inpari HDB dan Inpari Blas Ifa Manzila; Tri Puji Priyatno
Jurnal Fitopatologi Indonesia Vol 11 No 6 (2015)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Wild rice accession Oryza rufipogon is known as a source of tungro resistance genes and has been used to develop tungro resistant varieties, especially against rice tungro spherical virus (RTSV). However the genes have not been identified yet. Previously, an eukaryotic Translation Initiation Factor (eIF4G) was identified as a putative gene associated with recessive resistance gene against RTSV in Utri Merah variety. The research was aimed to detect and identify the eIF4G gene on rice var.Inpari HDB and var. Inpari Blas using specific primer that was designed based on Utri Merah eIF4G gene sequences. The resistance respons of both Inpari varieties and O.rufipogon against 3 tungro virus isolates were conducted in the green house trial. Assays were done based on international rice testing nursery according to IRRI. The eIF4G gene is amplified by PCR and the amplicon was directly sequenced, then analysed in silico. The results showed that all 3 varieties are classified as resistant against tungro virus isolates. PCR was successfully amplified the eIF4G gene with size ~300 bp in both of varieties and their parent O. rufipogon. The nucleotides homology of eIF4G among those 2 varieties and O. rufipogon is up to 100%, while the homology to Utri Merah was 93%. There were 4 nucleotides deletion and 16 nucleotides differences between Utri Merah and those 2 varieties and O. rufipogon, respectively. Those nucleotide differences lead to deletion of 1 amino acid and 4 amino acids different between both Inpari varieties and O.rufipogon in comparison with corresponding amino acid in Utri Merah.

Penghambatan Fusarium oxysporum oleh Kultur Filtrat Bakteri Endofit dari Tanaman Kedelai secara in Vitro Novi Malinda; Bonny Poernomo Wahyu Soekarno; Titiek Siti Yuliani
Jurnal Fitopatologi Indonesia Vol 11 No 6 (2015)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Seed borne pathogen play an important role as source of inoculum for disease incidence in the field and it becomes a major constraint in certified seed production. Research was conducted to isolate potential endophytic bacteria from soybean plants and evaluate its culture filtrate for inhibition effect of seedborne fungi on soybean seed, i.e. Fusarium oxysporum. The result showed that out of forty eight endophytic bacteria isolates that were nonpathogenic, there were three potential isolates that can inhibit the growth of F. oxysporum, i.e. EDA 3, EBA 6, and EBA 7 with percent inhibition of 60.14%, 57.69%, and 57.08%, respectively. The filtrate culture of EBA 7 showed the highest inhibition (34.88%) by in vitro test. Therefore, those three isolates of endophytic bacteria might be used as biocontrol agent to inhibit the growth of F. oxysporum.

Identifikasi Virus Penyebab Penyakit Kerdil pada Tanaman Padi di Sukamandi, Jawa Barat Amelia Feryna Bulan Dini; I Wayan Winasa; Sri Hendrastuti Hidayat
Jurnal Fitopatologi Indonesia Vol 11 No 6 (2015)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Viral diseases on rice is an important constraint for rice production in Indonesia. Research was conducted to determine the incidence of stunting disease in Sukamandi area at Subang regency (West Java), to identify the virus associated with the disease using RT-PCR method, and to analyze coat protein gene sequences. Field observation during growing period on November– December 2014 indicated that the incidence of stunting disease was low (0.01-10.52%). Grassy stunt and ragged stunt symptoms was observed in the fields. Spesific DNA fragments of coat protein gene of Rice ragged stunt virus (RRSV) and Rice grassy stunt virus (RGSV) was successfully amplified using specific primers. Nucleotide sequence analysis showed that RRSV and RGSV isolates from Subang has the highest homology with RRSV isolates from Vietnam, Philipines and Thailand (97.1 %) and RGSV isolate from Longan, Vietnam (95.8%), respectively.

Page 12 of 42 | Total Record : 412

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