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Jurnal Fitopatologi Indonesia

jfiti
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Published by Institut Pertanian Bogor

ISSN : 02157950 EISSN : 23392479 DOI : -

Core Subject : Health,

Medicine & Pharmacology

Jurnal Fitopatologi Indonesia (JFI) is an official publication owned by the Indonesian Phytopathology Society (Perhimpunan Fitopatologi Indonesia). In 2010, JFI management was given to PFI Komda Bogor. Since then, JFI has been published 6 times (January, March, May, July, September, and November).

Arjuna Subject : -

Articles 412 Documents

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Eksplorasi Bakteri Endofit dari Akar Tanaman Adam Hawa dan Potensinya sebagai Agens Hayati dan Pemacu Pertumbuhan Tanaman Padi Ankardiansyah Pandu Pradana; Diana Putri; Abdul Munif
Jurnal Fitopatologi Indonesia Vol 11 No 3 (2015)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Rhoeo discolor has been known to have a good adaptation to various environmental conditions. This character might be due to mutualistic association with endophytic bacteria. The objective of this study was to isolate endophytic bacteria from roots of R. discolor and to evaluate their potency as biocontrol agents and plant growth promoters. The methods to isolate endophytic bacteria involved the following methods, sterilization of root surface, grinding of root tissues, dilution, and plating in the medium tryptone soya agar (TSA). A total of 21 isolates of endophytic bacteria were isolated from the roots of R. discolor. Based on hypersensitivity test on tobacco leaves, 19 isolates showed negative reaction (no necrosis symptom) and only 2 isolates showed positive reaction (necrosis was developed). The results on biocontrol and growth promoters assay showed that 7 isolates were able to inhibit the growth of Fusarium oxysporum under in vitro test and 12 isolates were able to increase the growth of rice seedlings.

Deteksi Bakteri Penyebab CVPD pada Jeruk Menggunakan DNA Asal Tulang Daun Ummu Salamah Rustiani; Ariningsih Salji Endah; Nurjanah Nurjanah; Andi Prasetiawan; Nurmaida Nurmaida
Jurnal Fitopatologi Indonesia Vol 11 No 3 (2015)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

A method for identification of the causal bacteria of citrus vein phloem degeneration (CVPD) based on polymerase chain reaction (PCR) technique using template DNA extracted from leaf midrib of citrus has been implemented routinely. The method has not been validated, therefore it is necessary to validate the method to confirm that the method fit for its intended use. Leaf samples showing chlorotic symptom was obtained from Bogor and Bekasi, West Java and used for test samples. These samples was differentiated into 2 groups, i.e. leaf midrib and leaf mesophyll. Validation test involved homogenicity, and reproducibility test; each test was replicated 2 times. The test showed that using leaf midrib gave better result for detection of bacteria causing CVPD disease than using leaf mesophyll. Therefore, this method is recommended as routine detection method for bacteria causing CVPD disease.

Identifikasi Nematoda Parasit pada Tanaman Wortel di Dataran Tinggi Malino, Sulawesi Selatan Berdasarkan pada Ciri Morfologi dan Morfometrik Hishar Mirsam; Supramana Supramana; Gede Suastika
Jurnal Fitopatologi Indonesia Vol 11 No 3 (2015)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Plant-parasitic nematodes are important pests on carrot (Daucus carota) in Malino Highland. This research aimed to identify plant-parasitic nematodes on carrot. The identification was carried out based on the morphological and morphometric characters of second-stage juveniles that were extracted from soil samples. Nematodes were extracted using the flotation-centrifugation technique. Morphometric measurement included body length, stylet length, esophagus length from the basal knob to the esophagus end, tail length from the posterior end to the anus, anterior diameter, maximum body diameter, and posterior diameter. Three genera of plant-parasitic nematodes were identified as Meloidogyne, Rotylenchulus, and Pratylenchus.

Identifikasi Cendawan Penyebab Penyakit Pascapanen pada Beberapa Buah di Yogyakarta Ani Widiastuti; Ovianne Hapsari Ningtyas; Achmadi Priyatmojo
Jurnal Fitopatologi Indonesia Vol 11 No 3 (2015)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

In Indonesia, high yield losses due to post-harvest diseases are often difficult to measure because research focusing on such matter are still limited. This study aimed to determine the genera of fungi that cause rot on postharvest fruit, which can be used as a basis to determine the important pathogens in the current post-harvest commodities and for further disease management. The method used is sample collection, single spore isolation, microscopic observation and identification of fungal genera. Colletotrichum sp. was sucessfuly isolated from antrachnose of papaya. Pestalotia sp. was found in the fruit rot of avocado and star fruit. Lasiodiplodia sp. was found in mango, while Pestalotia sp. and Lasiodiplodia sp. was found in both sapodilla and banana. Alternaria sp. was found in the fruit rot of pears and apples. Aspergillus sp. was found in grapes, and Fusarium sp. was isolated from pineapple fruit rot.

Deteksi dan Identifikasi Cendawan Terbawa Benih Brassicaceae Anthoni Sulthan Harahap; Titiek Siti Yuliani; Widodo Widodo
Jurnal Fitopatologi Indonesia Vol 11 No 3 (2015)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Seed quality is very critical in agricultural production, especially to gain high yield and reduce disease problems in the field. New diseases or pathogens is potentially entering a country through seed movement by import activity. This study aimed to detect and identify seed-borne fungi from Brassicaceae seeds imported from the United States and Malaysia. Seeds were incubated on 5 sheets of wet blotting paper at a temperature of 27–30 °C for 14 days following surface sterilization. Each fungus that grows on the seed was isolated on potato dextrose agar and malt extract agar for further morphological identification. The three fungi most commonly found either on the seed with or without surface-sterilization were Aspergillus flavus, Curvularia lunata and A. niger. All of the fungi were a potential pathogen in the family Brassicaceae seeds and seedlings. Important pathogen in Brassicaceae crops, i.e. Phoma lingam was also found in small amounts and only on white pak choy seeds.

Deteksi dan Identifikasi Dickeya sp. sebagai Organisme Pengganggu Tumbuhan Karantina A2 pada Tanaman Kentang di Jawa Haerani Haerani; Abdjad Asih Nawangsih; Tri Asmira Damayanti
Jurnal Fitopatologi Indonesia Vol 11 No 4 (2015)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Erwinia chrysanthemi (currently Dickeya sp.) is one of the A2 quarantine pest that must be concerned of its distribution on potato in Indonesia. The aim of this study is to detect and identify E. chrysanthemi from potato in Java. A total of 400 samples of potato plants showing symptoms of soft rot were obtained from several potato areas in Pangalengan and Garut (West Java), Dieng (Central Java), and Batu-Malang (East Java). Disease incidence was determined by indirect enzyme-linked immunosorbent assay (I-ELISA) using polyclonal antiserum. E.chrysanthemi was isolated from plant samples with positive ELISA results. Furthermore, bacterial isolates were characterized by GEN III OmniLog ID System and PCR using specific primers Ec3F/Ec4R, as well as the universal 16S rRNA primer pair of 27F/1429R. The incidence of E. chrysanthemi based on ELISA was obtained. Based on physiological characters; Gram, catalase, oxidase, and oxidation-fermentation, there were 4 isolates similar to the genus of Erwinia. However, the results of Gen III OmniLog System, PCR, and nucleotide sequences analysis of 16S rRNA confirmed that none of the isolates were identified as E.chrysanthemi. Otherwise, those 4 isolates were identified as Pseudomonas oryzihabitans, Pantoea agglomerans, and Pseudomonas viridiflava. The result of this study indicated that the existence of E. chrysanthemi as an A2 quarantine pest on potato in Java can not be confirmed and remains as an A1 quarantine pest.

Deteksi Virus Utama Bawang Merah dan Bawang Putih dari Daerah Jawa Barat dan Jawa Tengah Kadwati Kadwati; Sri Hendrastuti Hidayat
Jurnal Fitopatologi Indonesia Vol 11 No 4 (2015)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Viral disease has been reported to cause significant effect on production of shallot (A. cepa) and garlic (A. sativum). The study was conducted to detect main viruses from leaves and bulbs of shallot and garlic using specific antibodies by ELISA method. Leaf and bulb samples was collected from West Java (Bandung, Bogor and Cirebon), Central Java (Brebes), and Yogyakarta (Bantul). Single as well as mix infection of GCLV, SLV, and Potyvirus was successfully detected using specific antibodies. The average percentage of virus infection in the crop ranged from 11.2–14.3% on shallot, and 14.3% on garlic; whereas in the bulb ranged from 11.2–13.3% on shallot, and 9.18% on garlic.

Perlakuan Panas Kering dan Bakterisida untuk Menekan Infeksi Pantoea stewartii subsp. stewartii pada Benih Jagung Manis Suswi Nalis; Gede Suastika; Giyanto Giyanto
Jurnal Fitopatologi Indonesia Vol 11 No 4 (2015)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Stewart’s Wilt is an important bacterial disease of sweet corn caused by Pantoea stewartii subsp. stewartii (synonim Erwinia stewartii). This bacteria is a seed transmitted pathogen therefore seed treatment is one method to control stewart’s wilt. The aim of this research was to study the effectiveness of dry heat, bactericide treatment, and their combinations to eliminate P. stewartii subsp. stewartii infection on sweet corn seed without damaging seed quality. The research was conducted in 3 experiments. Experiment I was conducted to determine the treatment window of dry heat and bactericide treatment. The treatment was carried out on sweet corn seed using the P. stewartii subsp. stewartii in vitro. Experiment II was conducted to study dry heat and bactericide treatment on sweet corn seed infested by P. stewartii subsp. stewartii. Experiment III was conducted to study combination of dry heat and bactericide treatment on sweet corn seed infested by P. stewartii subsp. stewartii. The results showed that dry heat treatment at 50 °C for 24 hours was able to eliminate pathogen populations in vitro but was unable to eliminate the 128 pathogen on infected seed (in vivo). Germination tests indicated that seed treatments with dry heat up to 55 °C did not decrease the germination level. The use of bactericide treatment in 100 ppm could reduce the population of bacteria on sweet corn seeds. Bactericide concentration of 150 and 200 ppm could decrease the population of bacteria on sweet corn seeds, however it could cause phytotoxic effect. The combination of bactericide (100 ppm, w/v) ) with dry heat treatment (55 °C for 24 hours) was able to eliminate bacteria on infected seed with seed germination above 85%.

Ekspresi Rekombinan Gen Protein Selubung Pepper vein yellows virus Rita Kurnia Apindiati; Gede Suastika; Kikin Hamzah Mutaqin
Jurnal Fitopatologi Indonesia Vol 11 No 4 (2015)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Pepper vein yellows virus (PeVYV) isolate from Bali have been identified from pepper plants with chlorosis symptoms. Specific antiserum of PeVYV had not available yet commercially. One of the advance techniques in providing a source of abundant antigen for antiserum production is through molecular approach by overexpressed the coat protein gene in suitable bacterial expression system. PeVYV coat protein gene of ~650 bp in size was amplified using specific primers, then was cloned into pQE30 expression vector and was over expressed in E. coli strain M15 [pREP4]. SDS-PAGE analysis showed that the recombinant coat protein gene of PeVYV was successfully expressed protein band with size of ~25 kDa at 6 hours after induction by 0.5 mM IPTG on 37 °C.

Identifikasi Nematoda Puru Akar, Meloidogyne graminicola, pada Tanaman Padi di Jawa Barat Mochamad Yadi Nurjayadi; Abdul Munif; Gede Suastika
Jurnal Fitopatologi Indonesia Vol 11 No 4 (2015)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Root knot nematodes (RKN), Meloidogyne spp. is one of the most important rice pathogen in some regions in the world. Information of RKN on rice plants in West Java is very important because West Java is one of the largest national rice production center. The purpose of this study was to determine and identify the presence of Meloidogyne spp. on rice plants in West Java. Rice plant samples were taken from 7 rice-growing districts in West Java. Typical symptoms of infected rice plants by Meloidogyne spp. are stunting and the formation of root galls. RKN was found to infect rice plants in several areas in Bogor, Cirebon, and Sukabumi. All stadia of the RKN development which include eggs, second, third, and fourth stage jeveniles, adult females and males were found inside the rice root tissues based on nematode staining observation. Morphometric measurements of the body and stylet length, maximum body width, length of the second stage juveniles, and female perineal pattern, indicated that Meloidogyne species found was M. graminicola.

Page 11 of 42 | Total Record : 412

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