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INDONESIA
Jurnal Fitopatologi Indonesia
Published by Institut Pertanian Bogor
ISSN : 02157950     EISSN : 23392479     DOI : -
Core Subject : Health,
Medicine & Pharmacology
Jurnal Fitopatologi Indonesia (JFI) is an official publication owned by the Indonesian Phytopathology Society (Perhimpunan Fitopatologi Indonesia). In 2010, JFI management was given to PFI Komda Bogor. Since then, JFI has been published 6 times (January, March, May, July, September, and November).
Arjuna Subject : -
Articles 412 Documents
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Potency of Endophytic Bacteria from Brotowali (Tinospora crispa) as Biocontrol of Sclerotium rolfsii and Plant Growth Promoting Abdul Munif; Kholil Ma’ruf
Jurnal Fitopatologi Indonesia Vol. 16 No. 3 (2020)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14692/jfi.16.3.95-104

Abstract

Sclerotium rolfsii merupakan salah satu patogen penting pada kacang tanah yang dapat menyebabkan penyakit busuk pangkal batang. S. rolfsii dapat bertahan hidup di dalam tanah dan membentuk struktur dorman. Penelitian ini bertujuan mengevaluasi bakteri endofit dari tanaman brotowali yang berpotensi sebagai agens hayati yang efektif untuk mengendalikan S. rolfsii dan mengetahui pengaruhnya pada pertumbuhan tanaman kacang tanah. Bakteri endofit diisolasi dari akar dan batang tanaman brotowali dengan metode sterilisasi permukaan. Bakteri endofit yang berhasil diisolasi dari tanaman brotowali ialah 415 isolat dan 153 isolat lolos uji keamanan hayati. Sebanyak 7 isolat bakteri endofit, yaitu BBT25, BBT90, BBT102, BBT106, BBT110, BBT130, dan BSK18 berpotensi menekan S. rolfsii. Isolat BBT106 mampu menekan pertumbuhan S. rolfsii sebesar 73% secara in vitro. Isolat BBT110 dan BSK18 mampu menekan kejadian penyakit busuk pangkal batang sebesar 58% dan 67% pada penelitian di rumah kaca. Ketujuh isolat bakteri endofit brotowali mampu meningkatkan pertumbuhan tanaman kacang tanah pada percobaan di rumah kaca.
Potensi Beberapa Isolat Bakteri Endofit untuk Pengendalian Biologi Meloidogyne graminicola pada Tanaman Padi abdul munif; Mohammad Yadi Nurjayadi
Jurnal Fitopatologi Indonesia Vol 17 No 1 (2021)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14692/jfi.17.1.28-34

Abstract

Meloidogyne graminicola merupakan salah satu nematoda parasit penting pada tanaman padi. M. graminicola menyebabkan gejala puru pada akar sehingga pertumbuhan tanaman terhambat dan terganggu. Pengendalian M. graminicola sangat penting dilakukan agar kerusakan pada tanaman padi dan penyebarannya dapat ditekan. Penelitian ini bertujuan untuk menguji potensi bakteri endofit dalam mengendalikan nematoda puru akar (NPA) M. graminicola pada tanaman padi. Empat isolat bakteri endofit asal tanaman padi, yaitu isolat Si33, Si2, Sp24, GH1, dan satu isolat asal tanaman kentang G053 diuji untuk melihat pengaruhnya terhadap mortalitas M. gramincola secara in vitro dan kemampuannya dalam menekan serangan M. graminicola pada tanaman padi di rumah kaca. Hasil uji kultur filtrat bakteri endofit secara in vitro terbukti efektif meningkatkan kematian juvenil 2 M. graminicola sebesar 99.5% hingga 100%. Isolat bakteri endofit juga mampu menekan jumlah puru akar pada tanaman padi dengan kisaran 42.2%–49.3% pada percobaan di rumah kaca. Hasil penelitian ini mengindikasikan bahwa isolat bakteri endofit yang digunakan dalam penelitian ini mempunyai potensi yang baik sebagai agens biokontrol untuk mengendalikan M. graminicola pada tanaman padi.
Trichoderma Asal Akar Kopi Dari Alor: Karakterisasi Morfologi dan Keefektifannya Menghambat Colletotrichum Penyebab Penyakit Antraknosa secara in Vitro Didiana Yanuarita Molebila; Ade Rosmana; Untung Surapaty Tresnaputra
Jurnal Fitopatologi Indonesia Vol 16 No 2 (2020)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14692/jfi.16.2.61-68

Abstract

Trichoderma of Coffee Roots From Alor: Morphological characteristic and in vitro Efficacy to Inhibit Colletotrichum Causing Anthracnose Trichoderma is a fungus capable of intimate associations with plant root systems including on coffee plants. This aim of study is to determine the characteristics of Trichoderma morphospecies from coffee roots of Alor origin, East Nusa Tenggara (NTT) and its ability to inhibit the growth of Colletotrichum causing anthracnose disease in-vitro. Root samples of healthy coffee plants were taken from the location of coffee plantations in Alor District, NTT. Isolation of Trichoderma fungi from coffee roots was done by incubating the sterilized coffee roots in a layer of moist filter paper in a Petri dish for seven days. Identification of Trichoderma by observing the characteristics of the colony on the medium of potato dextrose agar (PDA) and microscopic media using microcultures (slide culture). Inhibition of Trichoderma fungi against Colletotrichum was tested by multiple culture methods on PDA media. The results of root incubation in humid conditions showed that there was four morphospecies of Trichoderma fungi, each of which had different characteristic specifications. In vitro antagonism in test on PDA medium, the first three morphospecies against Colletotrichum showed that each Trichoderma could inhibit 70.2%, 65.8%, and 63.3%, respectively, five days after inoculation. This data shows that Trichoderma isolated from coffee roots from Alor has the potential to suppress the growth of anthracnose pathogens.
Uromyces acori (Uredinales) Penyebab Nekrosis pada Daun Tanaman Jeringau (Acorus calamus) di Indonesia Dono Wahyuno; Marlina Puspita Sari; Dini Florina
Jurnal Fitopatologi Indonesia Vol 16 No 2 (2020)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14692/jfi.16.2.81-86

Abstract

Uromyces acori (Uredinales) A Causal Agent of Rust Disease of Sweet Flag (Acorus calamus) in Indonesia In Indonesia, an asexual state of a rust fungus namely Uredo acori has been considered as the causal agent rust disease of the sweet flag. No report according sexual state is available in Indonesia. The objectives of the present study are confirming the fungal identity morphologically and determine its optimal germinating temperature. An artificial inoculation was conducted by dusting the urediniospore onto detached leaves of sweet flag. Only leaves showed uredinia were selected. The tip bases of the leaves were dipped into a 4% sucrose solution, incubated in a room condition at 25 °C for inducing telial state with teliospore formations. Based on morphological characters of the teliospore and urediniospore, the rust fungus of sweet flag was identified as Uromyces acori. The fungus is germinating optimally at 25 °C.
Wakatobi Native Bacteria Inhibit The Colony Growth of Alternaria porri and Fusarium oxyporum of Shallots in in Vitro Study Gusti Ayu Kade Sutariati; Andi Khaeruni; Abdul Madiki
Jurnal Fitopatologi Indonesia Vol. 16 No. 3 (2020)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14692/jfi.16.3.105-111

Abstract

Wakatobi Native Bacteria Inhibit The Colony Growth of Alternaria porri and Fusarium oxyporum of Shallots in in Vitro Study Biological control of plant diseases based on the usage of microbes is important to reduce the use of chemical pesticides. The aim of this study was to determine the inhibitory and inhibitory mechanism in vitro of native endophytic bacterial isolates from Wakatobi as biological control of fusarium wilt and purple spots on shallot plants. The research was carried out at the Agronomy Unit of Agrotechnology Laboratory, Agriculture Faculty, Halu Oleo University Kendari. The research was aranged in a Completely Randomized Design (RAL), consisting of 9 treatments of endophytic bacterial isolates, namely Be03, Be02, Ke03, Ke05, Te01, Te05, Re05, Re05, and Wae05. The experiment was repeated 3 times wih 27 treatment units in total. The inhibitory test of endophytic bacteria on the tested pathogens was carried out in 2 steps, namely (1) against Alternaria porri and (2) Fusarium oxysporum. In addition to observing the inhibition mechanisme may involve. The ability of the endophytic bacteria isolates in producing HCN compounds was also tested. The results showed that endophytic bacteria had the ability to inhibit the growth of pathogenic fungi A. porri and F. oxysporum. Among the 9 isolates tested, there were 2 endophytic bacterial isolates which had the best ability to inhibit the growth of pathogenic A. porri and F.oxysporum fungi namely Te05 and Be03. Be03 isolates also have a high ability in producing HCN compounds, but no for Te05. Further research is needed to assay the effectiveness of those 2 isolates as biological control for diseases of shallot in a larger scale in the field.
Pathogenicity of Endophytic Fusarium oxysporum Isolated from Weeds in Banana Plantations against Bananas Seedlings var. Raja Bulu Vinsen Willi Wardhana; Suryo Wiyono; Sri Hendrastuti Hidayat; Widodo Widodo
Jurnal Fitopatologi Indonesia Vol 17 No 1 (2021)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14692/jfi.17.1.1-8

Abstract

Fusarium oxysporum merupakan penyebab penyakit layu dan banyak mematikan tanaman pisang. Cendawan ini diketahui mampu hidup dan bertahan sebagai endofit pada gulma dan menjadi sumber inokulum yang nantinya menyebabkan penyakit pada tanaman pisang. Penelitian ini dilakukan untuk mengisolasi F. oxysporum dari akar gulma yang tumbuh di sekitar pertanaman pisang di Bogor, Jawa Barat, kemudian menguji sifat patogenisitasnya. Identifikasi morfologi dilakukan dengan mengamati koloni, konidium, dan klamidosporanya. Uji patogenisitas isolat endofit dilakukan di laboratorium menggunakan bibit pisang var. Raja Bulu. Sebanyak 9 isolat F. oxysporum berhasil diisolasi dari gulma yang tumbuh di sekitar pertanaman pisang. Semua isolat menunjukkan ciri morfologi sebagai F. oxysporum, yaitu koloni berwarna ungu, makrokonidium berbentuk kano dengan tiga sekat false head pendek, mikrokonidium berbentuk silindris atau ginjal, dan klamidospora umumnya tersusun tunggal. Semua isolat menyebabkan insidensi penyakit sebesar 100% pada bibit pisang var. Raja Bulu dengan keparahan gejala layu pada daun antara 58.33% sampai 77.78% dan nekrosis pada bonggolnya antara 50.00% sampai 69.44%.
Pengamatan dan Pemetaan Penyakit Busuk Pangkal Batang di Perkebunan Kelapa Sawit Menggunakan Unmanned Aerial Vehicle (UAV) dan Kamera Multispektral Heri Santoso
Jurnal Fitopatologi Indonesia Vol 16 No 2 (2020)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14692/jfi.16.2.69-80

Abstract

Surveillance and Mapping of Basal Stem Rot Disease in Oil Palm Plantation Using Unmanned Aerial Vehicle (UAV) and Multispectral Camera Basal stem rot (BSR) disease caused by Ganoderma boninensis is still a major disease in oil palm plantations both in Indonesia and Malaysia. In some countries, remote sensing approach has been used for monitoring BSR in oil palm plantation. However, the utilization of satellite imagery in remote sensing especially in vegetation study on the tropical region was often limited by cloud cover. A drone or unmanned aerial vehicle (UAV) utilization is the best way to deal with cloud cover in the tropic region. Machine learning of random forest (RF) and satellite imagery used in the BSR study produced good accuracy. This research was aimed to identify and monitor the BSR infection on individual oil palm trees using an UAV and multispectral camera and RF classification. The results showed that the data acquired from UAV was affected by cloud shadows. The RF classification of healthy and infected oil palm trees by BSR disease and the spreading map of BSR infection was affected by cloud shadows. The highest accuracy of healthy and infected oil palm by BSR was 79.49%. Reflectance calibrator, digital to reflectance conversion, and model implications to build spreading map of BSR infection need to be conducted both on the clear area and the cloud shadow-covered area. Moreover, the UAV-based data should be considering the cloud view on the coverage area.
Konjugat Poliklonal Antibodi Nanopartikel Emas untuk Deteksi Potato Virus Y Ifa Manzila; Tri Puji Priyatno; Fikri Hidayatullah
Jurnal Fitopatologi Indonesia Vol 16 No 2 (2020)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14692/jfi.16.2.87-93

Abstract

Polyclonal Antibodi-Gold Nanoparticles for Potato Virus Y Detection Gold nanoparticles are stable colloidal solutions with dimensions of 1-100 nm having surface plasmon resonance with six free electrons. The existing of six free electrons on the surface of a plasmon causes gold nanoparticles to bind easily to various types of bioreseptors including polyclonal antibodies. Polyclonal potato virus Y (PVP) antibodi been successfully conjugate with gold nanoparticles in order to develop a rapid detection for PVY infection in potato plants. The gold nanoparticles was synthetized by the reduction of gold (III) chloride trihydrate (HAuCl4) with 1% sodium citrate. Subsequently, the nanoparticles were used to make gold nanoparticle-antiobody PVY-conjugate. PVY detection was carried out with dot blot method on the nitrocellulose membrane. The results showed that the PVY virus on the membrane can be detected 10-30 minutes after incubation, depend on the concentration of the conjugate and the concentration of the virus in the sampel. The use of gold nanoparticle conjugates can increase the efficiency of the immunodot blot method in about 1 hour, and this method can be developed to be a lateral flow system for field detection of PVY.
Pengendalian Burkholderia glumae pada Benih Padi dengan Perlakuan Panas Kering dan Minyak Cengkeh Kresnamurti Kurniasih; Giyanto Giyanto; Meity Suradji Sinaga; Kikin Hamzah Mutaqin; Eny Widajati
Jurnal Fitopatologi Indonesia Vol. 16 No. 3 (2020)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14692/jfi.16.3.123-134

Abstract

Control of Burkholderia glumae in Rice Seed by Dry Heat and Clove Oil Treatments Burkholderia glumae is a seed-borne pathogen that causes bacterial grain rot (BGR) ​disease in rice. This study aims to obtain ​disease in rice. This study aims to obtain B. glumae control techniques in rice seeds using a combination of dry heat and clove oil treatment without reducing the quality of rice seeds. This research consisted of several stages, namely: 1) preparation of healthy and infected seeds of B. glumae, 2) determination of the temperature and duration range of treatment that effectively reduced of the B. glumae population without reducing the viability of healthy seeds, 3) the effect of dry heat treatment on population B. glumae and seed viability of infected seed, 4) determination concentration of clove oil that decreases B. glumae population without decreasing seed viability of healthy seed, 5) the effect of clove oil treatment on B. glumae population and seed viability of infected seed and 6) combination of dry heat and clove oil treatment to control B. glumae in infected seed. The study showed that B. glumae population in rice seeds reduced as much as 97.68%, 98.00% dan 99.38% by dry heat treatment at 55 °C for 3, 4, and 5 hours without decreasing seed germination (seeds germination respectively 92%, 94% dan 93%). Application of clove oil 0.5% and 0.75% were able to reduce the bacterial population of 86.61% and 98.26% with the seed germination of 90.25% and 90.00%. The combination of 0.75% clove oil treatment followed by dry heat treatment at 55 °C for 5 hours eliminated all B. glumae in rice seeds without reducing the seed germination
Evaluation of Plant-based Pesticide containing Neem Extract (Azadirachta sp.) to Control Anthracnose Growth in Chili Fruits Yashanti Berlinda Paradisa; Wahyuni; Enung Sri Mulyaningsih; Ambar Yuswi Perdani; Arief Heru Prianto
Jurnal Fitopatologi Indonesia Vol. 16 No. 3 (2020)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14692/jfi.16.3.112-122

Abstract

Cabai merupakan salah satu produk hortikultura yang banyak dikonsumsi di Indonesia. Antraknosa merupakan salah satu penyakit utama dalam budi daya cabai. Kehilangan hasil akibat antraknosa mencapai 35%. Penelitian ini bertujuan untuk menentukan efektivitas pestisida nabati dengan bahan utama ekstrak mimba dalam mengendalikan antraknosa pada cabai. Penelitian ini dilakukan di Pusat Penelitian Bioteknologi, LIPI. Pengujian in vitro dilakukan menggunakan rancangan acak kelompok faktorial yang diulang 4 kali dengan faktor pertama ialah cendawan Colletotrichum acutatum dan Colletotrichum gloeosporioides; faktor kedua ialah pestisida nabati dengan bahan aktif mimba (Agr I dan Agr II); serta faktor ketiga berupa 6 taraf perlakuan konsentrasi pestisida (0%, 0.5%, 1%, 2%. 3%, 4%, dan 5%). Pada pengujian in vivo dilakukan menggunakan rancangan acak kelompok faktorial dengan 4 ulangan. Faktor pertama ialah cabai besar dan cabai keriting; faktor kedua ialah C. acutatum dan C. gloeosporioides; dan faktor ketiga ialah 4 taraf konsentrasi pestisida Agr I (0%, 5%, 10, dan 15%). Berdasarkan hasil pengujian diketahui bahwa pestisida nabati Agr I dan Agr II dapat menghambat pertumbuhan cendawan secara in vitro dan Agr I lebih potensial untuk mengendalikan Colletotrichum spp. Namun pestisida nabati Agr I tidak mampu mengendalikan patogen yang telah berada di dalam jaringan tanaman.

Page 25 of 42 | Total Record : 412

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