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Juvenil: Jurnal Ilmiah Kelautan dan Perikanan
Published by Universitas Trunojoyo Madura
ISSN : -     EISSN : 27237583     DOI : -
Core Subject : Science, Agriculture, Social,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Environmental Science
Juvenil: Journal of Marine and Fisheries Sciences, is a scientific journal in the field of marine and fisheries science published electronically and periodically four times a year by the Department of Marine Affairs and Fisheries, Trunojoyo University, Madura. This journal aim to become a medium of dissemination of high quality research and scientific note in the fiend of marine and fisheries. This journal can be accessed and downloaded freely for everyone. The article published in this journal have been pass the rigorious peer review by the expert reviewer. This journal accept every article that contain the following, but not limited to, scope: Juvenil: Journal of Marine and Fisheries Sciences, is a scientific journal in the field of marine and fisheries science published electronically and periodically four times a year by the Department of Marine Affairs and Fisheries, Trunojoyo University, Madura. This journal is expected to function as a medium for the dissemination of quality scientific research results as well as scientific rebuttal (notes) in the marine and fisheries sector which can be accessed online and free of charge by the Indonesian community and the international community. The articles (articles) published in this journal are articles that have passed peer-review (partner bebestari). This journal accepts every article which contains, but is not limited to, the scope: 1. Ecology and biology of marine and fisheries 2. Marine and Aquaculture 3. Marine and Aquatic Conservation 4. Marine Water Pollution 5. Management of marine and aquatic resources 6. Marine and fishery processing technology
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Perairan
Articles 10 Documents
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Search results for , issue "Vol 4, No 2: Mei 2023" : 10 Documents clear
Ekstraksi pada Daun Jeruju Acanthus ilicifolius Alviona Rahmazsanti; Maulinna Kusumo Wardhani; Askur Rahman
Juvenil Vol 4, No 2: Mei 2023
Publisher : Department of Marine and Fisheries, Trunojoyo University of Madura, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21107/juvenil.v4i2.18504

Abstract

ABSTRAKTumbuhan Jeruju Acanthus ilicifolius pada hutan mangrove memiliki nilai obat yang belum sepenuhnya dieksplorasi. Penelitian ini bertujuan untuk mendapatkan dan membandingkan antara ekstrak daun jeruju Acanthus ilicifolius dari Kabupaten Bangkalan serta Kabupaten Probolinggo yang berpotensi dalam pengembangan obat alternatif. Beberapa penelitian terdahulu menyatakan ekstrak jeruju sebagai antimikroba karena kandungan senyawanya dapat memerangi penyakit, terutama pada bagian daun. Penelitian ini mengekstraksi daun jeruju dalam memisahkan kandungan senyawanya dengan bantuan pelarut yang akhirnya akan menghasilkan suatu ekstrak dan selanjutnya dianalisis karakteristiknya serta dihitung nilai persen rendemennya yang kemudian akan diuraikan secara deskriptif. Hasil penelitian menunjukkan serbuk daun jeruju yang digunakan sebagai ekstrak daun jeruju Acanthus ilicifolius Kabupaten Bangkalan memiliki karakteristik sampel yang berwarna hijau kecoklatan, bertekstur lebih kasar, memiliki rasa yang pahit dan berbau ciri khas, sedangkan serbuk daun jeruju yang digunakan sebagai ekstrak daun jeruju Acanthus ilicifolius Kabupaten Probolinggo berwarna hijau, bertekstur lebih halus, juga memiliki rasa pahit serta berbau ciri khas. Selain itu, ekstrak daun jeruju Kabupaten Bangkalan menunjukkan nilai persen rendemen yang lebih tinggi sebesar 1,2% dibandingkan dengan ekstrak daun jeruju Kabupaten Probolinggo yang memiliki nilai persen rendemen sebesar 0,6% sehingga menghasilkan kesimpulan bahwa ekstrak jeruju Acanthus ilicifoius Socah lebih baik kandungan senyawanya dari ekstrak jeruju Acanthus ilicifolius Probolinggo dan berpotensi dalam pengembangan obat alternatif. Kata kunci: Ekstraksi, Jeruju Acanthus ilicifolius, Ekstrak daun jeruju ABSTRACTThe Jeruju plant Acanthus ilicifolius in mangrove forests has a medicinal value that has not been fully explored. This study aims to obtain and compare Acanthus ilicifolius jeruju leaf extract from Bangkalan Regency and Probolinggo Regency which have the potential in developing alternative medicines. Several previous studies stated that orange extract is antimicrobial because the compounds it contains can fight disease, especially in the leaves. This study extracted Jeruju leaves by separating the compound content with the help of a solvent which would eventually produce an extract then analyzed its characteristics and calculated the percent yield value which would then be described descriptively. The results showed that the orange leaf powder used as an extract for Acanthus ilicifolius leaves for Bangkalan Regency had the characteristics of a brownish-green color, a rougher texture, a bitter taste, and a characteristic odor, while the powder for Jeruju leaves used as an extract for Acanthus ilicifolius leaves for the Regency of Probolinggo is green in color, has a finer texture, also has a bitter taste and a characteristic odor. In addition, the jeruju leaf extract of Bangkalan Regency showed a higher yield percent value of 1.2% compared to the orange leaf extract of Probolinggo Regency which had a yield percent value of 0.6%, leading to the conclusion that Acanthus ilicifoius Socah orange extract had better compound content. from the orange extract of Acanthus ilicifolius Probolinggo and has potential in the development of alternative medicine. Keywords: Extraction, Jeruju Acanthus ilicifolius, Jeruju leaf extract
Sebaran Ukuran, Hubungan Panjang-Berat, dan Faktor Kondisi Ikan Julung-Julung (Nomorhamphus sp.) di Air Terjun Nanga-nanga Kota Kendari, Sulawesi Tenggara Muhammad Nur Findra; Sjamsu Alam Lawelle; La Ode Muhammad Arsal; Daniel Frikli Mokodongan; Yustika Intan Permatahati; Risfandi Risfandi; Muhamad Ikbal; Sapri Sapri
Juvenil Vol 4, No 2: Mei 2023
Publisher : Department of Marine and Fisheries, Trunojoyo University of Madura, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21107/juvenil.v4i2.19213

Abstract

ABSTRAKIkan julung-julung (Nomorhamphus sp.) merupakan salah satu jenis dari familia Zenarchopteridae. Ikan ini teramati di sekitar air terjun Nanga-nanga Kota Kendari dengan jumlah populasi yang kecil. Studi ini bertujuan untuk mengkaji sebaran ukuran, hubungan panjang-berat, dan faktor kondisinya di air terjun Nanga-nanga. Ikan ditangkap menggunakan jaring, dipisahkan berdasarkan jenis kelamin kemudian diukur panjangnya menggunakan mistar dengan ketelitian 0,1 cm dan ditimbang beratnya menggunakan timbangan digital dengan ketelitian 0,01 gram. Hasil penelitian menunjukkan sebaran ukuran tertinggi dari semua ikan yang dikumpulkan adalah pada kelas ukuran 4,2-6,5 cm, sedangkan pada ikan jantan ukuran tertinggi pada kelas ukuran 5,76-6,12 cm dan ikan betina tertinggi pada kelas ukuran dan 4,25-6,5 cm. Hubungan panjang-berat ikan julung-julung yang ditemukan menunjukkan bahwa ikan tersebut memiliki pola pertumbuhan yang bersifat allometrik positif (b3). Nilai faktor kondisi yang didapatkan 0,9945±0,0809 untuk ikan jantan, 1,0171±0,0862 untuk ikan betina, dan 0,9907±0,0807 untuk gabungan ikan jantan-betina. Baik ukuran maupun faktor kondisi, ikan betina memiliki kondisi yang lebih baik. Penelusuran lebih lanjut terhadap keberadaan ikan ini diperlukan untuk menentukan rencana pengelolaannya.Kata Kunci: air terjun Nanga-nanga, faktor kondisi, hubungan panjang-berat, Nomorhamphus sp., sebaran ukuranABSTRACTHalfbeak fish (Nomorhamphus sp.) is a type of the Zenarchopteridae family. It was observed around the Nanga-nanga waterfall, Kendari City, with a small population. This study was aimed to examine the size distribution, length-weight relationship, and condition factors at Nanga-nanga waterfall. The fish were caught using nets, separated by sex, then measured their length using ruler with an accuracy of 0.1 cm and weighed using a digital scale with an accuracy of 0.01 gram. The results showed that the highest size distribution of all fish collected was in the 4.2-6.5 cm size class, while the highest size for male and female were 5.76-6.12 and 4.25-6.5 cm size class, respectively. The relationship between fish  length  and weight was  found showed  that they have a positive  allometric  growth  pattern (b3). Condition factor values obtained for male, female, and both were 0.9945±0.0809, 1.0171±0.0862, and 0.9907±0.0807, respectively. Both size and condition factors, female have better conditions. Further investigation of this fish existence is needed to determine its management plan.Keywords: Nanga-nanga waterfall, condition factor, length-weight relationship, Nomorhamphus sp., size distribution
Analisis Kadar Residu Antibiotik Kloramfenikol Pada Udang Vannamei (Litopenaeus Vannamei) Di Kabupaten Bangkalan Dengan Metode Elisa (Enzym Link Immunosorbent Assay) Jihan Mayang Sari; Hafiludin Hafiludin
Juvenil Vol 4, No 2: Mei 2023
Publisher : Department of Marine and Fisheries, Trunojoyo University of Madura, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21107/juvenil.v4i2.18075

Abstract

ABSTRAKPenggunaan antibiotik pada budidaya udang masih dilakukan. Antibiotik yang sering digunakan yaitu kloramfenikol yang merupakan antibiotik berspektrum luas dalam aktivitas untuk melawan bakteri aerobik, anaerobik, dan fungi. Tujuan dari peneltian ini adalah mengetahui adanya kandungan kloramfenikol dan mengetahui kadar kloramfenikol pada udang vannamei. Metode yang digunakan yaitu metode ELISA (Enzym link immunosorbent assay) untuk menganalisis kandungan dan kadar kloramfenikol pada udang vannamei. Hasil penelitian yang diperoleh yaitu terdapat kandungan kloramfenikol pada semua sampel udang vannamei dengan perubahan warana menjadi kuning. Kadar residu yang diperoleh dari Kwanyar sebesar 0,006 ppb, Sepulu sebesar 0,027 ppb dan Socah sebesar 0,014 ppb. Hal ini menunjukkan bahwa udang vannamei di tiga lokasi tersebut masih aman untuk dikonsumsi dan di ekspor karena tidak melebihi BMR yang ditetapkan SNI 01- 6366-2000 yaitu sebesar 0,01 ppm dan Uni Eropa sebesar 0,3 ppb.Kata Kunci: Antibiotik, Kloramfenikol, Udang vannamei, ELISAABSTRACTThe use of antibiotics in shrimp cultivation is still being carried out. The most commonly used antibiotic is chloramphenicol. Chloramphenicol is a broad-spectrum antibiotic with activity against aerobic, anaerobic, and fungal bacteria. The purpose of this study was to determine the content of chloramphenicol and to determine the levels of chloramphenicol in vannamei shrimp. The ELISA (Enzyme link immunosorbent assay) to analyze the content and levels of chloramphenicol in vannamei shrimp. The results obtained were that there was chloramphenicol content in all vannamei shrimp samples with a change in color to yellow. The residual level obtained from Kwanyar was 0,006±0,0044 ppb, Sepulu was 0,027±0,0172 ppb and Socah was 0,014±0,0026 ppb. It can be concluded that vannamei shrimp in the three locations are still safe for consumption and export because they do not exceed the BMR set by SNI 01-6366-2000 which is 0,01 ppm and the European Union is 0,3 ppb.Keywords: Antibiotics, Chloramphenicol, Vannamei Shrimp, ELISA
Developing The Salt Centre of Excellence as a Unique Selling Proposition University of Trunojoyo Madura Makhfud Efendy; Wiwit Sri Werdi Pratiwi; Aries Dwi Siswanto
Juvenil Vol 4, No 2: Mei 2023
Publisher : Department of Marine and Fisheries, Trunojoyo University of Madura, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21107/juvenil.v4i2.20171

Abstract

AbstractAs one of the most enormous salt globally, Indonesia consumes more than 3 million tonnes of salt annually, mainly imported from several countries. However, Indonesia is the second-longest equatorial coastline in the world, with abundant salt resources from the sea. Developing a salt industry development centre on Madura Island is a crucial solution to eradicate Indonesia's label as a salt imported dependent state. Madura Island has large salt production and is labelled a salt island. Being a centre of academics on Madura Island, the University of Trunojoyo Madura is qualified as a salt centre of Excellence due to having met thorough research and development, sourcing absorptive and disseminating capacity guidelines. Research and academic activities in the salt field, such as attending scientific seminars and conferences, participating in workshops and other centers of excellence collaboration, have been done in a decade as academic Excellence. Several industries have produced and utilized the commercialization of research products like fortified salt, rich mineral salt, and solus per aqua (SPA) salt. University also develops collaborative research, advisory and informal knowledge exchange between the university and industry. To implement this program's success, the university develops information systems technology to manage the Centre of excellent performances. Finally, based upon the work done on several guidelines, the university's positive impact on research, industrial corporation, and product dissemination has increased dramatically over the years. Keywords: salt centre of excellent, unique selling proposition, University of Trunojoyo Madura
Pengaruh Perbedaan Modul Transplantasi Karang Terhadap Pertumbuhan Karang di Pulau Pramuka Abdul Malik; La Ode Alam Minsaris; Luthfi Anzani
Juvenil Vol 4, No 2: Mei 2023
Publisher : Department of Marine and Fisheries, Trunojoyo University of Madura, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21107/juvenil.v4i2.19675

Abstract

ABSTRAKSebaran terumbu karang di Indonesia sekitar 85% terancam rusak dan 50% lainnya mengalami ancaman kerusakan yang tinggi. Pada wilayah perairan Pulau Pramuka menunjukkan persentase tutupan karang hidup sebesar 20,65%-47,17% yang dikategorikan sedang hingga rusak. Apabila tidak segera ditangani, ini bisa berdampak buruk bagi ekosistem laut. Perlu adanya upaya restorasi agar dapat membantu pemulihan terumbu karang yang mengalami kerusakan. Oleh karena itu, penelitian ini bertujuan untuk membandingkan pertumbuhan karang pada ketiga modul transplantasi karang yang berbeda yaitu antara modul rocklife, PVC, dan spider agar lebih mengefektifkan dalam menentukan modul terbaik yang ingin digunakan pada kondisi perairan yang sama seperti Pulau Pramuka. Parameter perairan yang diukur meliputi suhu, kecerahan, pH, salinitas, oksigen terlarut, dan kecepatan arus. Penelitian dilakukan selama 4 bulan dengan menggunakan 60 sampel fragmen karang dari spesies Acropora sp. yang ditanam pada masing-masing modul transplantasi karang. Metode yang digunakan dalam penelitian ini adalah eksperimen dengan observasi selama 3 bulan dan analisis korelasi pearson serta metode statistik dengan regresi linear digunakan untuk memodelkan hubungan antara parameter oseanografi tertentu dengan pertumbuhan karang pada masing-masing modul. Hasil penelitian menunjukkan selama kurun waktu 3 bulan, parameter perairan yang diukur tidak menunjukkan perbedaan yang signifikan dalam mempengaruhi pertumbuhan karang. Namun, hasil penelitian menunjukkan adanya korelasi antara pH dan DO dengan pertumbuhan karang. Secara keseluruhan perbandingan dari ketiga modul transplantasi yang dilakukan di perairan Pulau Pramuka mendapatkan hasil, modul rocklife menjadi metode yang terbaik.Kata Kunci: Laju pertumbuhan, Modul transplantasi, Pulau Pramuka, Terumbu karang, Transplantasi KarangABSTRACTThe distribution of coral reefs in Indonesia is around 85% threatened with damage, with the other 50% experiencing a high threat of damage. In the waters of Pramuka Island, the percentage of live coral cover is 20.65%–47.17%, which is included in the moderate to damaged category. If not addressed immediately, this can have a negative impact on the marine ecosystem. Restoration efforts are needed to help recover damaged coral reefs. Therefore, this study aims to compare coral growth in three different coral transplant modules, namely between rocklife, PVC, and spider modules, to further streamline determining the best module to use in the same water conditions as Pramuka Island. The water parameters measured included temperature, brightness, pH, salinity, dissolved oxygen, and current speed. The research was conducted for 4 months using 60 samples of coral fragments from Acropora sp. species planted in each coral transplant module. The method used in this study was experimental, with observations for 3 months, and Pearson correlation analysis and statistical methods with linear regression were used to model the relationship between certain oceanographic parameters and coral growth in each module. The results showed that over a 3-month period, the measured water parameters did not show significant differences in influencing coral growth. However, the results showed a correlation between pH and DO  and  coral  growth.  Overall,  the  comparison  of  the  three transplantation modules conducted in the waters of Pramuka Island resulted in the rocklife module being the best method.Keywords: Coral reefs, Coral transplantation, Growth rate, Pramuka island, Transplant modules
Fortifikasi Tepung Tulang Ikan Bandeng (Chanos chanos) Pada Kue Kembang Goyang Siti Nur Alisa; Andi Noor Asikin; Seftylia Diachanty; Irman Irawan; Ilmiani Rusdin; Indrati Kusumaningrum
Juvenil Vol 4, No 2: Mei 2023
Publisher : Department of Marine and Fisheries, Trunojoyo University of Madura, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21107/juvenil.v4i2.20053

Abstract

ABSTRAKIkan bandeng (Chanos chanos) merupakan salah satu jenis ikan budidaya yang dapat ditemukan di perairan payau. Produksi budidaya ikan bandeng yang semakin meningkat setiap tahunnya, menyebabkan meningkatnya limbah yang masih belum dimanfaatkan secara optimal, salah satunya tulang ikan. Industri pengolahan bandeng tanpa duri menghasilkan hasil samping berupa duri, tulang dan sisik. Tulang ikan merupakan hasil samping yang kurang dimanfaatkan dan mengandung sekitar 85% mineral yaitu berupa kalsium fosfat. Salah satu bentuk pemanfaatan tulang ikan yaitu mengolahnya menjadi tepung dengan melakukan fortifikasi pada kue kembang goyang. Penelitian ini bertujuan untuk  mengetahui karakteristik fisikokimia kue kembang goyang  dengan fortifikasi tepung tulang ikan bandeng. Penelitian ini menggunakan rancangan acak lengkap (RAL) dengan perlakuan penambahan persentase tepung tulang ikan bandeng (0%, 2%,  4%, 6%) pada kue kembang goyang. Parameter yang diuji pada penelitian ini adalah kadar air, kadar abu, kadar protein, kadar lemak, kadar karbohidrat, kadar kalsium, dan higroskopisitas. Hasil penelitian menunjukkan bahwa fortifikasi tepung tulang ikan bandeng memberikan pengaruh nyata terhadap uji fisikokimia. Hasil analisis fisikokimia pada kadar air (1,49-2,65%), abu (0,94-1,61%), lemak (3,44-4,04%), protein (5,03-5,38%), karbohidrat (87,04-88,15%),  kalsium (0,39-1,10%) dan higrokopositas (2,70-6,15g).Kata kunci : Fortifikasi, Ikan bandeng, Kalsium, Kembang Goyang, Tepung Tulang Ikan.ABSTRACTMilkfish (Chanos chanos) is a cultivated fish found in brackish water. Milkfish aquaculture production increased every year, causing a massive waste that has not processed optimally, one of the waste is fish bones. The boneless milkfish processing industry produces by products in the form of spines, bones and scales. Fish bone is a by product that is underutilized and contains about 85% of minerals, namely calcium phosphate. One form of utilizing fish bones is to process them into flour by fortifying the kembang goyang crackers.The purpose of this study were to the physicochemical characteristics of kembang goyang crackers with milkfish bone flour fortification. This study used a completely randomized design (CRD) with the addition of the percentage of milkfish bone meal (0%, 2%, 4%, 6%) in the kembang goyang crackers. The parameters tested in this study were moisture, ash, protein, fat, carbohydrate, and calcium content, also hygroscopicity. The results showed that the fortification of milkfish bone meal had a significant effect on the physicochemical tests. Physicochemical analysis results on moisture content (1.49-2.65%), ash (0.94-1.61%), fat (3.44-4.04%), protein (5.03-5.38%), carbohydrates (87.04-88.15%), calcium (0.39-1.10%) and hygrocopositivity (2.70-6.15g).Keywords: Calcium, Fish Bone Meal, Fortification, Kembang Goyang, Milkfish.
Transfection Of Difficult-To-Transfect Zebrafish (Danio rerio) ZF4 Cells Using Chemical Transfection and Nucleofection Method Rizka Rahmana Putri; Christian Larbi Ayisi
Juvenil Vol 4, No 2: Mei 2023
Publisher : Department of Marine and Fisheries, Trunojoyo University of Madura, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21107/juvenil.v4i2.19586

Abstract

ABSTRACTTransfection is a powerful tool for introducing foreign DNA into target cells. Many kits and methods are offered to simplify the process of introducing foreign DNA into cells. ZF4 is a cell type derived from zebrafish that is difficult to transfect. The majority of studies involving transfection into cells require transfection efficiency 70%. Our research demonstrates transfection using 2 kinds of methods, namely chemical transfection using X-tremeGene HP and electroporation-based transfection using a nucleofector device which will later be called Nucleofection. Our results show that transfection using electricity (Nucleofection) provides better transfection efficiency than chemical transfection (X-tremeGene HP).Keywords: maxGFP, nucleofection, transfection, zebrafish (Danio rerio), ZF4 cells
Pengaruh Kombinasi Pakan dan Probiotik yang Berbeda Terhadap Kinerja Pertumbuhan Udang Galah (Macrobrachium rosenbergii) dan Penurunan Limbah N yang Dipelihara Dalam Wadah Terkontrol Gamal Mustik Samadan; Fatma Muchdar; Sriwati Sriwati; Muhammad Nur Findra
Juvenil Vol 4, No 2: Mei 2023
Publisher : Department of Marine and Fisheries, Trunojoyo University of Madura, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21107/juvenil.v4i2.18389

Abstract

ABSTRAK                                                           Udang Galah (Macrobranchium rosenbergii) merupakan salah satu jenis crustacea, yang mempunyai ukuran terbesar dibandingkan dengan udang air tawar lainnya. Penggunaan probiotik terhadap perbaikan mutu kualitas air kinerja pertumbuhan hewan budidaya sudah lazim digunakan. Namun demikian, pada budidaya udang galah masih sangat terbatas informasinya. Penelitian ini bertujuan mengetahui jenis probiotik yang berpengaruh dalam budidaya udang galah (M. rosenbergii), yang dipelihara dalam wadah terkontrol. Penelitian dilaksanakan selama 60 hari di UPT Laboratorium Terpadu Universitas Khairun, Kota Ternate Provinsi Maluku Utara. Penelitian dirancang dengan rancangan acak lengkap (RAL) yang terdiri dari empat perlakuan dan tiga kali ulangan. Perlakuan yang dicobakan adalah probiotik yang berbeda, yaitu probiotik boster planktop (10 ml), probiotik boster sel multi (10 ml), probiotik boster bio lakto (10 ml), dan tanpa. Hasil analisis menunjukkan pemberian probiotik boster planktop signifikan berpengaruh pada kelangsungan hidup udang galah, sedangkan pertumbuhan mutlak, laju pertumbuhan spesifik dan rasio konversi pakan tidak signifikan. Disamping itu, pemberian probiotik yang berbeda menurunkan konsentrasi amoniak dan nitrit, dan juga mempertahankan kualitas air media pemeliharaan.Kata kunci: Probiotik, pertumbuhan, limbah N, udang galahABSTRACTGiant Shrimp (Macrobranchium rosenbergii) is one type of crustacean, which has the largest size compared to freshwater shrimp. The use of probiotics to improve water quality, growth performance of cultivated animals is commonly used. However, the information on giant prawn cultivation is still very limited. This study aims to determine the types of probiotics that have an effect on the cultivation of giant prawns (M. rosenbergii), which are kept in controlled containers. The research was carried out for 60 days at the UPT Integrated Laboratory of Khairun University, Ternate City, North Maluku Province. The study was designed with a completely randomized design (CRD) consisting of four treatments and three replications. The treatments tested were different probiotics, namely planktop booster probiotics (10 ml), multi cell booster probiotics (10 ml), bio lacto booster probiotics (10 ml), and without. The results of the analysis showed that different probiotics had an effect on the survival of giant prawns, while absolute growth, specific growth rate and feed conversion ratio were not significant. In addition, the administration of different probiotics reduces the concentration of ammonia and nitrite, and also maintains the water quality of the maintenance media.Keywords: Probiotics, growth, waste removal, giant prawns
Analisis Karakter Morfometrik Ikan Sumera (Tor Tambroides) di Perairan Aek Sibundong Kabupaten Tapanuli Tengah Lauura Hermala Yunita; Fiki Harjuni; Rizky Janatul Magwa; Farhan Ramdhani
Juvenil Vol 4, No 2: Mei 2023
Publisher : Department of Marine and Fisheries, Trunojoyo University of Madura, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21107/juvenil.v4i2.20059

Abstract

ABSTRAKIkan Sumera (Tor tambroides) termasuk dalam family Cyprinidae yang hidup di perairan air tawar salah satunya hidup di perairan Aek Sibundong Kabupaten Tapanuli Tengah. Penelitian ini bertujuan mengevaluasi kemungkinan penggunaan karakter morfometrik untuk melihat perbedaan antara ikan jantan dan betina pada ikan Sumera. Penelitian ini dilaksanakan pada bulan Desember tahun 2022 menggunakan metode simple random sampling. Identifikasi morfologi ikan dilakukan dengan menggunakan pengukuran karakter morfometrik. Pengukuran karakter morfometrik ikan mengacu pada Smith (1945). Jumlah sampel didapatkan sebanyak 30 ekor ikan Sumera, 15 ekor ikan jantan memiliki kisaran panjang total yaitu 17.5 – 30.3 cm dengan kisaran berat 50-315 g dan 15 ekor ikan betina memiliki kisaran panjang total yaitu 15.6 - 30.3 cm dengan kisaran berat 30-315 g. Berdasarkan hasil uji t terhadap proporsi karakter morfometrik ikan Sumera antara ikan jantan dan betina tidak jauh berbeda nyata. Hanya terdapat 4 karakter morfometrik yang berbeda yaitu pada bagian panjang dasar sirip anal (ABL), lebar badan (BW), panjang sebelum sirip anal (PAL), dan panjang sirip dada (PCL).Kata Kunci: Ikan Sumera (Tor tambroides), karakter morfometrik, perairan Aek SibundongABSTRACTThe Sumera fish (Tor tambroides) belongs to the Cyprinidae family which lives in fresh water waters, one of which lives in the waters of Aek Sibundong, Central Tapanuli Regency. This study aims to evaluate the possibility of using morphometric characters to see differences between male and female fish in Sumera fish. This research was conducted in December 2022 using the simple random sampling method. Identification of fish morphology was carried out using morphometric character measurements. Measurement of fish morphometric characters refers to Smith (1945). The number of samples obtained was 30 Sumera fish, 15 male fish had a total length range of 17.5 – 30.3 cm with a weight range of 50-315 g and 15 female fish had a total length range of 15.6 - 30.3 cm with a weight range of 30-315 g. Based on the results of the t test on the proportion of morphometric characters of Sumera fish, the male and female fish were not significantly different. There were only 4 different morphometric characters namely anal fin length (ABL), body width (BW), length before anal fin (PAL), and pectoral fin length (PCL).Keywords: Aek Sibundong waters, morphometric characters, Sumerian fish (Tor tambroides)
Pemeriksaan WSSV (White Syndrome Virus) Dengan Uji PCR (Polymerase Chain Reaction) Pada Udang Vannamei (Litopenaeus vannamei) di UPT Laboratorium Kesehatan Ikan dan Lingkungan, Pasuruan Jawa Timur Amalia Khofifah; Indah Wahyuni Abida; Asmaul Khusna
Juvenil Vol 4, No 2: Mei 2023
Publisher : Department of Marine and Fisheries, Trunojoyo University of Madura, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21107/juvenil.v4i2.16462

Abstract

ABSTRAKUdang vannamei mempunyai banyak keunggulan daripada udang jenis lainnya, akan tetapi resisten terhadap serangan penyakit yang disebabkan oleh virus. Virus WSSV merupakan virus yang menyerang sistem organ dari crustacea yang menyebabkan spot putih atau bercak putih di permukaan eksternal udang sehingga menimbulkan kerugian berupa kematian tinggi mencapai 100% dari udang pemeliharaan dalam waktu 3-10 hari sejak gejala klinis muncul. Metode pemeriksaan WSSV (White Spot Syndrome Virus) pada udang vannamei dilakukan dengan uji PCR (Polymerase Chain Reaction). Prosedur diawali persiapan sampel, ekstraksi sampel, persiapan reagen, amplifikasi pada mesin PCR, persiapan elektroforesis, proses elektrophoresis, analisis hasil pada UV Transilluminator, dokumentasi hasil dan pembacaan hasil. Penelitian ini dilaksanakan di UPT Laboratorium Kesehatan Ikan dan Lingkungan, Pasuruan Jawa Timur dari tanggal 17 Januari sampai 17 Februari 2022 dengan tujuan mengetahui hasil pemeriksaan WSSV dengan uji PCR pada udang vannamei. Pemeriksaan sampel 59 dan 61 menunjukkan hasil WSSV negatif dengan adanya 1 garis perpendaran pita DNA (band) ukuran 848 bp, sampel tersebut tidak terinfeksi virus WSSV. Pembacaan hasil uji PCR (+) WSSV berat (severe) terlihat 3 garis perpendaran pita DNA (band) ukuran 910 bp, 550 bp, 296 bp, yang terjadi pada sampel 57 dan sampel duplo 57. Sampel 72 menunjukkan hasil (+) WSSV ringan (light) yang terlihat 1 garis perpendaran pita DNA (band) dengan ukuran 296 bp. Kata Kunci: Udang vannamei, WSSV (White Spot Syndrome Virus), PCR (Polymerase Chain Reaction) ABSTRACTVannamei shrimp has many advantages over other types of shrimp, but this shrimp is resistant to disease caused by viruses. WSSV virus is a virus that attacks the organ systems of crustaceans which causes white spots or white spots on the external surface of shrimp, causing losses in the form of high mortality reaching 100% of reared shrimp within 3-10 days since clinical symptoms appear. The WSSV (White Spot Syndrome Virus) examination method in vannamei shrimp can be done by using the PCR (Polymerase Chain Reaction) test. The procedure begins with sample preparation, sample extraction, reagent preparation, amplification on a PCR machine, electrophoresis preparation, electrophoresis process, analysis of results on UV Transilluminator, documentation of results and reading of results. This research was conducted at UPT Fish and Environmental Health Laboratory, Pasuruan, East Java from January 17 to February 17 2022 with the aim of knowing the results of the WSSV examination with PCR test on vannamei shrimp. Examination of samples 59 and 61 showed negative WSSV results with 1 fluorescent DNA band (band) measuring 848 bp, the samples were not infected with the WSSV virus. The reading of the heavy (severe) WSSV PCR test results showed 3 fluorescent lines of DNA bands (bands) measuring 910 bp, 550 bp, 296 bp, which occurred in sample 57 and sample duplo 57. Sample 72 showed a result of (+) mild WSSV (light) which shows 1 fluorescent line of DNA band (band) with a size of 296 bp. Keywords: Vannamei Shrimp, WSSV (White Spot Syndrome Virus), PCR (Polymerase Chain Reaction)

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