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Berkala Penelitian Hayati

jurnal
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Published by Perhimpunan Biologi Indonesia Cabang Jawa Timur

ISSN : 08526834 EISSN : 2337389X DOI : https://doi.org/10.23869/bphjbr

Core Subject : Health, Science, Agriculture,

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology

Berkala Penelitian Hayati is a half yearly international peer reviewed, an open access life science journal. The journal was published by The East Java Biological Society and formerly used the Indonesian language. The first edition of this journal is Vol 1 No 1 in June 1995. It was accredited by Ministry of Culture and Education. It continues recorded by Zoological Record by Thomson Reuters Clarivate Analytics since 2011. Since April 2012, the journal was changed into English. This journal is indexed by DOAJ, Crossref, Google Scholar, Academia.edu, and EBSCO Host. This journal publishes original research, applied, review article, and educational articles in all areas of biology. Authors are encouraged to submit complete unpublished and original works that are not under review in other journals. This journal publishes original research, applied, review articles, and educational articles in all areas of biology. Authors are encouraged to submit complete unpublished and original works that are not under review in other journals. The journal scopes include, but are not limited to, the following topic areas including botany, zoology, ecology, microbiology, physiology, nanobiology, coastal biology, hydrobiology, neurobiology, genetics, developmental biology, biochemistry and molecular biology, biophysics, and life science.

Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)

Articles 507 Documents

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Tissue repair in post extraction sockets of Cavia cobaya induced by a combination of propolis and graft Utari Kresnoadi; Roselini Halim; Hananah Oktalidial Putri; Mutiara Aryanita; Imam Safari Azhar
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 25 No 2 (2020): June 2020
Publisher : The East Java Biological Society

Propolis, hive defensive substance that contains caffeic acid phenethyl ester, can affect the migration and proliferation of cells involved in the healing process. Combination of propolis extract with BBG are predicted to fasten the wound healing, especially its effect on angiogenesis, macrophages and collagen density. In this study, we aimed to analyze the effect of a propolis combination and BBG in proliferating new blood vesselsand macrophages, while also increasing the collagen density of the post-extracted tooth socket healing process in Cavia cobaya. 56 C. cobaya, whose left mandibular incisorshad been extracted, were subsequently divided into four groups on the basis of the substances used to fill their sockets, namely; control, BBG, propolis extract, and a combination of BBG, and propolis extract. The subjects sacrificed on either the third or seventh day. Histopathological samples were produced using HE and MT staining. The results were subjected tosome statistical analysis tests: one-way analysis of variance (ANOVA), Tukey’s honestly significant difference (HSD), Kruskal Wallis, and Mann Whitney. Significant increases (p<0.05) were detected between the PEG and PEG+Propolis groups and also between the PEG and PEG+BBG+Propolis combination groups. However, an insignificant increase (p>0.05) was identified between the PEG and BBG groups. In conclusion, a combination of BBG and propolis can increase the number of new blood vessels and macrophages together with the degree of collagen density in the healing process of post-extraction tooth sockets of C. cobaya.

IN VITRO CULTURE OF ORCHIDS: THE ROLES OF CLASS-1 KNOX GENE IN SHOOT DEVELOPMENT Endang Semiarti; Aziz Purwantoro; Ari Indrianto
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 20 No 1 (2014): December 2014
Publisher : The East Java Biological Society

In vitro culture of orchids has been developed for many purposes. Some native orchids and commercial orchid hybrids are propagated using seed germination or cut explants such as leaves, shoot tips, and roots to produce large numbers of orchid plantlets. This technique is widely used for the purpose in conservation of natural orchid species and industry of commercial orchid hybrids. However, the molecular genetic mechanism behind growth and development of these orchids during in vitro culture is still unclear, and needs to be elaborated. Recent advanced in transgenic technology in orchid is very helpful for studying the mechanism of action of key genes in various stages of orchid development during in vitro culture. In this review, an attempt to understand the role of class-1 KNOX gene and its relationship with other genes in the initiation of shoot apical meristem (SAM) for shoot development from orchid protocorm (a tubercle of developing orchid embryo) and PLBs (Protocorm Like Bodies) during in vitro culture will be discussed. It will answer the question about how the shoot formation can be controlled during growth and development of orchid cells in in vitro culture.

THE REPRODUCTIVE PERFORMANCE OF BALI CATTLE AND IT’S GENETIC VARIATION Sri Rahayu
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 20 No 1 (2014): December 2014
Publisher : The East Java Biological Society

Bali cattle ( Bos sondaicus ) is one of the Indonesia native cattle which is the result of wild banteng domestication. The advantages of Bali cattle, among others, are having a high fertility rate (80-82 %) and good adaptability to the new environment. It was found that there are genetic variation in several genes associated with reproductive function in Bali cattle. Some studies have reported an association between genetic variation in reproductive function of cattle. However, studies on the correlation between genetic variation reproductive genes with reproductive performance of Bali cattle are still low. This article aims to provide an overview of Bali cattle genetic variation reproductive genes and making it possible as candidate marker for selection and improving reproductive performance of Bali cattle.

Dendrobium spectabile (Blume) Miq. IN VITRO CULTURE AND ITS ACCLIMATIZATION ON MUS MEDIA WITH ANTIMICROBIAL AND ALCOHOLIC SUGAR SUPPLEMENTATION Untung Santoso; Y.Sri Wulan Manohara; Kusriningrum Rochiman S.
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 20 No 1 (2014): December 2014
Publisher : The East Java Biological Society

Germination and development of orchid seed of Dendrobium spectabile through in vitro method has been done with good result and yielded many seeds. Different acclimatization approach was developed with sphagnum moss medium which supplemented with the same nutrition as MUS medium. Alcoholic sugar was supplemented to increase medium moisture and antimicrobial was added to minimalized contamination which can harm orchid seeds. Planlet adaptation ability to drought stress which resulted from in vitro culture can be improved using the new medium. The medium will increase the probability of seed survival also it will normalize seed development through the acclimatization process.

THE INFLUENCE OF Aloe vera AND XENOGRAFT (XCB) TOWARD OF BONE MORPHO PROTEIN 2 ( BMP2) EXPRESSION AND AMOUNT OF OSTEOBLAST OF ALVEOLAR BONE INDUCED INTO TOOTH EXTRACTION SOCKETS (Cavia cobaya) Utari Kresnoadi; Retno Pudji Rahayu
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 19 No 2 (2014): June 2014
Publisher : The East Java Biological Society

Tooth extraction can cause inflammation leading to alveolar ridge resorption. In addition, prominent ridge has crucial role for making denture successfully. Thus, socket preservation is needed to prevent greater alveolar ridge resorption. An innovative material, a combination of Aloe vera and xenograft (XCB), is then considered as a biogenic stimulator that can reduce inflammation, as a result, the growth of alveolar bone is expected to be improved. This research is aimed to prove whether the mixture of Aloe vera and xenograft can stimulate BMP2 and increase osteoblasts. Forty-eight Cavia cobaya animals were divided into eight groups each of which consisted of six animals. The mandibular incisors of those Cavia cobaya animals were then extracted and filled with PEG as Group Control, XCB as Group XCB, Aloe vera as Group Aloe vera, and a combination of Aloe vera +XCB as Group Aloe vera +XCB. Next, the first four groups were sacrificed seven days after extraction, and the second four groups were sacrificed 30 days after extrac-tion. And then, immunohistochemical and histopathology examinations were conducted to examine BMP2 expression and osteoblasts. Based on the re-sult known that the mixture of Aloe vera and xenograft can increase BMP2 expression and amount of osteoblasts. It can be concluded that the mixture of Aloe vera and xenograft can increase BMP2 expression and amount of osteoblast cel . It can be used as an alternative material to increase the growth of alveolar bone after extraction.

PCR APPROACH FOR RAPID DETECTION OF Escherichia coli IN TEMPE USING A SPECIFIC PRIMER Siti Harnina Bintari; Fidia Fibriana; Dewi Mustikaningtyas; Retno Sri Iswari
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 19 No 2 (2014): June 2014
Publisher : The East Java Biological Society

Tempe known as a traditional fermented food originated from Indonesia. It has a unique flavour and texture. It also contains high protein and usually serves to substitute meat, fish, or egg as a complement to rice. The manufacture process of Tempe is quite complex and mostly, the traditional process has not employed the hygienic standard. In the process of Tempe making, there are two critical stages of the whole process; i.e. soaking of soybeans and solid state fermentation by Rhizopus sp. During the process, foodborne pathogen bacteria such as Escherichia coli could contaminate the product of Tempe. The bacterial contamination could be revealed through culture dependent methods which is costly, laborious, and time consuming. Therefore, the culture-independent method such as polymerase chain reaction using a specific primer could be applied to detect target microorganism to save time and labour. In this study, thirty-one Tempe samples collected from different manufacturers in Semarang, Central Java, Indonesia were analysed by PCR. In order to obtain the bacterial genomic DNA, a modified Chelex 100-Microwave method was employed. The results of DNA extraction showed that the method was an applicable method. It gave high quantity and quality of DNA; therefore, it could be applied in the PCR reaction. The DNA samples were employed in PCR for detection of Escherichia coli using Ecoli706F/R. It was found that 27 out of 31 samples were detected having Escherichia coli contamination showed by the presence of the amplified product size 706 bp. The application of this method could significantly reduce costs and time of analysis in the laboratory. Further response after E. coli were detected could be employed, including investigation of the critical factors in Tempe manufacturing process which allowed E. coli contamination.

POLYMORPISM ANALYSIS OF IL17RA GENE TO THE IL17RA CONCENTRATION AND CHRONICITY DIFFERENCES IN NEFRITIS LUPUS NL PATIENTS Mahrus Ismail; Hani Susanti; Widodo Widodo
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 19 No 2 (2014): June 2014
Publisher : The East Java Biological Society

Nefritis lupus (NL) is autoimmune disease and one of serious complication from Systemic Eritematosus Lupus (LES). Clinical manifestation of NL was variated, there are hematuria microscopic asymptomatic until renal failure. In addition of clinical manifestation, the NL disorder degree also measured from histopathological grade. Although, the mechanism that mixed up with pathogenesis of NL was known, however the cytokine also play a role to the disease process. The cytokine that assumpted have a role to the inflammation is IL-17RA, the increase production of IL-17RA influenced to the in-flammation and NL chronicity degree. The aim of this study to analyzed the relationship between polymorphism of IL-17RA gene, blood IL-17RA con-centration with the NL disorder degree. These studies were used cross sectional with control case design. The sample were used 40 patients consist NL pa-tients and Lupus patient without nephritis as a control. The polymorphisms of IL-17RA gene were investigated by using PCR method and gene sequence analysis. The alterations of allele frequency of IL-17RA gene were analyzed by bioinformatics method. The bloods IL-17RA concentration were in-vestigated by ELISA method. The NL chronicity degrees were investigated by NL histopathological grade. The hypothesis were proofed by data normality test and homogeneity test, Chi-square and Odds ratio, Spearman correlation by using SPSS 17.0 for windows. The result of showed that there are sig-nificantly different between IL-17RA gene mutant genotype frequency of NL patient (NL) with the control patient with value OR 8.48. There are sig-nificantly different between G allele of IL-17RA gene of NL patient (case) with the control patient with the value OR 4.17. There are no significantly di-fferent between IL-17RA concentrations of NL patient (case) with the different chronicity value. There are positive correlation with the OR value 4.17 bet-ween IL-17RA concentrations with the chronicity value index.

A STUDY OF TYPE AND INTENSITY OF DISEASE INFECTING BANANA PLANTS Musa sp AT TEGALAGUNG VILLAGE SEMANDING SUBDISTRICT Supiana Dian Nurtjahyani; Devi Shyntya Agustin
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 19 No 2 (2014): June 2014
Publisher : The East Java Biological Society

Diseases affecting banana plants are very detrimental to farmers as these can lower production and economic income. The purpose of this study was to determine the type and intensity of the disease affecting banana plants. This research was an observational analytic study that observe and analyze condition or symptoms of diseases affecting banana plants in Tegalagung village, Semanding subdistrict, Tuban as many as 38 samples. Parameters observed were type of disease and measure intensity of the disease, data obtained were analyzed descriptively. Based on the symptoms that occurred on the leaves, the study found four disease types affecting banana plant that were fusarium wilt, bacterial wilt (Blood), Sigatoka leaf spot and stunting disease. The diseases intensity were 50% of Fusarium wilt; 26,66% of bacterial wilt (Blood); 26.32% of Sigatoka leaf spot and 15.38% of stunting disease. Conclusion of the study, the highest intensity of the disease that attacks banana plants is Fusarium wilt as high as 50%.

SLAUGHTER HOUSE SOLID WASTE MANAGEMENT IN INDONESIA Rhenny Ratnawati; Yulinah Trihadiningrum
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 19 No 2 (2014): June 2014
Publisher : The East Java Biological Society

he solid slaughter house waste (SSW) in Indonesia is generally disposed of into open dumped landfill. This type of solid waste can cause odor and atmospheric pollution if discharged directly into the environment. Additionally, it may spread disease due to the nesting vectors, and the resulting leachate can lead to groundwater contamination. This paper reviews the characterization of slaughter house (SH) types and SSW generation potential and to review the development of treatment technology of SSW and its application. The SH in Indonesia is divided into 3 classes, namely: 1) SH for large and small ruminants; 2) SH for poultry; 3) SH for pigs. Application technologies in Indonesia include compost and biogas technologies, and the use of rumen content for animal feed. Problem in biogas technology is generally caused by the high nitrogen content in the SSW. The most suitable raw material for biogas production is herbivore waste. The main advantages of using SSW for compost production are: the appropriate characteristics for composting process, free of hazardous contaminant, and appropriate composting technologies are available to reduce environmental problems caused by SSW. In addition, rumen content is considered to be a potential alternative for animal feed because have high content of amino acids (approximately 73.4% of the total protein) and rich in vitamin B complex. Among the disadvantages, the composting process of SSW requires long time period and generate air pollutants, such as ammonia and hydrogen sulphide.

Scavenging activity nano complex compounds of kelor (Moringa oleifera Lamk.) leaves and seeds Rafida Azizah; Tintrim Rahayu; Ari Hayati; Gatra Ervi Jayanti
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 26 No 1 (2020): December 2020
Publisher : The East Java Biological Society

Moringa oleifera Lamk. is a good source of natural antioxidants because it contains various types of antioxidant compounds such as ascorbic acid, flavonoids, phenolics, and carotenoids. Those antioxidant components forming complex structure have transitional metal as central compound, which have free radical scavenging activity. This study aims to determine the active compounds that act as scavenger in leaves and seeds of M. oleifera. The possible compound found in leaves-seeds is elaborated by in silico analysis, using Dr. Duke's Phytochemical and Ethnobotanical Databases, by mean Pass online, and HitPick software. The results of in silico analysis 3 compounds identified in the leaves that had a high antioxidant role, namely beta-carotene, kaempferol, quercetin, and 2 compounds in seeds that had a high antioxidant role, namely alpha-tocopherol, beta-carotene. The results of this study indicate that the antioxidant activity of the 3 treatments had differences effectiveness of antioxidants. All of these antioxidants has ability to bind transitional metal to form free radical scavenger.

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Home Page

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Contact Name
Sutiman Bambang Sumitro

Contact Email
berkalahayati@yahoo.com

Phone
+62341570631

Journal Mail Official
wulidanisa@berkalahayati.org

Editorial Address
Jalan Surakarta No. 5 Malang, Indonesia

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Abstract

Abstract

Abstract

Abstract

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Abstract

Abstract

Abstract

Abstract

Abstract

Home Page

OAI Link

Editorial Team

Contact

Reviewer

Google Scholar

Contact Name Sutiman Bambang Sumitro

Contact Email berkalahayati@yahoo.com

Phone +62341570631

Journal Mail Official wulidanisa@berkalahayati.org

Editorial Address Jalan Surakarta No. 5 Malang, Indonesia

Location Unknown, Unknown INDONESIA

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Contact Name
Sutiman Bambang Sumitro

Contact Email
berkalahayati@yahoo.com

Phone
+62341570631

Journal Mail Official
wulidanisa@berkalahayati.org

Editorial Address
Jalan Surakarta No. 5 Malang, Indonesia

Location
Unknown,

Unknown

INDONESIA