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All Journal HAYATI Journal of Biosciences El-Hayah : Jurnal Biologi Jurnal Matematika dan Ilmu Pengetahuan Alam Journal of Tropical Biodiversity and Biotechnology Jurnal Biodjati BIOTROPIC The Journal of Tropical Biology Jurnal Layanan Masyarakat (Journal of Public Service) Jurnal Riset Biologi dan Aplikasinya Cakrawala Repositori Imwi Jurnal Pengabdian kepada Masyarakat Nusantara Narra J Berkala Penelitian Hayati Folia Medica Indonesiana

Alfiah Hayati

Department Of Biology, Faculty Of Science And Technology Universitas Airlangga

Author-ID : 278745

Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Neuroscience Public Health Social Sciences Other

Published : 25 Documents Claim Missing Document

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Pengaruh Kadmium Terhadap Struktur Histologis Insang Ikan Lele (Clarias Batrachus) Hayati, Alfiah
Jurnal Matematika dan Ilmu Pengetahuan Alam Vol 17, No 2 (2015)
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (50.452 KB)

This study determined the effect of cadmium (Cd) on the structure of the gills of catfish (Clarias batrachus). Animals were exposed to 0 (control), 1, 2, and 4 ppm of Cd during 14 days. The results showed that Cd affected the structure of the gills of catfish, such as edema, hyperplasia, and fusion gill lamela. The gills of control animals showed the lowest damage (13.9%), where as the level of gill damage of fish exposed to 1, 2, and 4 ppm were 45.7; 71.6; and 82.1% respectively.Keywords: cadmium, catfish, edema, hyperplasia, gills.

PENGARUH POLISAKARIDA KRESTIN DARI EKSTRAK JAMUR Coriolus versicolor TERHADAP PROFIL PROTEIN TERSTIKULER DAN KADAR TESTOSTERON Mus musculus Astuti Wahyuningsih, Sri Puji; Gibson, Virid; Hayati, Alfiah
El-Hayah : Jurnal Biologi Vol 5, No 4 (2016): EL-HAYAH (VOL 5, NO 4,Maret 2016)
Publisher : Department of Biology Science and Technology Faculty UIN Maulana Malik Ibrahim Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18860/elha.v5i4.3476

The purpose of this research was to determine the effect of polysaccharide krestin (PSK) on the testicular protein profiles and testosterone levels of Mus musculus with variety of dosages. This research used a completely randomized design. It were devide into four treatment group i.e. control group, PSK treatment at a dose of Â 15, 30, 60 mg/kgBW. Each group had six replications. Testicular proteins were isolated by flushing technique and analized by SDS-PAGE. Testosterone levels were analized using ELISA technique at wavelength 450 nm. Protein bands analysis showed that there were no diversification between four treatments. Molecular weight of protein bands were 87, 63, 57, 35, and 30 kDa. The results of research showed that the testosterone levels at dosage 60 mg/kgBW had significanly different with control, PSK treatment of 15 and 30 mg/kgBW. PSK treatment of Â 60 mg/kgBW had lowest level at dosage, i.e. 25946.8 Ïg/mL. It can be concluded that giving variety of dosages of polysaccharide krestin did not affect to testicular protein profiles but giving effect to testosterone levels of Mus musculus.

Phytochemical Screening and Antibacterial Activity of Acorus calamus L. Extracts Bayyinatul Muchtaromah; Alfiah Hayati; Erna Agustina
Jurnal Biodjati Vol 4, No 1 (2019): May
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v4i1.4235

Staphylococcus aureus and Escherichia coli are among the most common species of gram-positive and gram-negative bacteria, which cause vaginitis, in infertile women. The Calamus rhizome (Acorus calamus L.) is an Indonesian plant that has antibacterial properties that can be used to treat vaginitis and increase fertility. The aim of this study was to determine the phytochemical and antibacterial activity of the calamus rhizoma in polar, semi-polar and non-polar solvents in the growth of S. aureus and E. coli. The antibacterial activity test was in the form of inhibitory test using the Kirby-Bauer, Minimum Inhibi-tion Concentration (MIC) and Minimum Bactericidal Concentration (MBC) by microdilution method with multilevel dilution (concentra-tions 50; 25; 12.5; 6.25; 3.13; 1.56; 0.78; and 0.39%). The screening results showed that ethanol and n-hexane extract contained alkaloids and triterpenoids, while chloroform extract was only triterpenoid. Chloroform extract produced the largest inhibition zone diameter of S. aureus and E. coli (7.26 and 3.28 mm), followed by ethanol extract (5.90 and 3.07 mm) and n-hexane extract (5.33 and 2.95 mm). The concentrations of 0.39 and 0.78% were the values of MIC and MBC for all three extracts, indicating that the extract of the calamus rhizome with several solvents in this study had the same antibacterial activity.

The Effect Of Cadmium on Sperm Quality and Fertilization Of Cyprinus carpio L. Alfiah Hayati; Khusnita Giarti; Yuli Winarsih; Muhammad Hilman Fuadil Amin
Journal of Tropical Biodiversity and Biotechnology Vol 2, No 2 (2017): December
Publisher : Universitas Gadjah Mada

The objective of the study was to determine the effect of cadmium on sperm quality and fertilization of C. carpio L. Sperm and eggs were collected by abdomen striping from the mature testis and ovary of C. carpio L. This study used one control and four treatment groups of variation on the cadmium concentration (0, 50, 100, 150, and 200 ppm) with eight replications. Sperm motility (mass motility, mass motility duration, and individual motility duration) and viability were measured after three to four seconds of incubation in the water. The percentage of fertility success was calculated by observing embryo development after the eggs were mixed with sperm and incubated in the water for 72 hours. The success of the fertilization process was indicated by a color change of the egg that darkens after successful fertilization, and white-milk if failed. The data were analyzed using analysis of variance (α = 0.05). The results of this study indicate that exposure of 50 ppm cadmium and control group shown success in term of sperm quality (motility and viability) and fertilization, but at 100 ppm or more decreased the sperm quality and fertilization rate. It can be concluded that cadmium exposure decreases sperm quality and fertility at 100 ppm or higher concentrations.

Metallothionein analysis and cell damage levels on the liver and gill Of Barbonymus gonionotus In Brantas River, Indonesia Alfiah Hayati; Nita Yuliarini; Agoes Soegianto; Hana Widyana; Inesavira Rindaputri; Nuris Auliya; Putri Ayu Ika
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 23 No 1 (2017): December 2017
Publisher : The East Java Biological Society

This study aimed to analyze the levels of metallothionein and damage to hepatocytes and gills of Barbonymus gonionotus in the Brantas River (upstream and downstream), and the correlation levels of metallothionein in the gills and liver of Barbonymus gonionotus with different sampling time variation (March, June, and September 2016). Fishes were caught using trawl in two stations (Karangkates Reservoir and the Kali Jagir river). Fish gills and livers were taken for histopathological analysis and levels of metallothionein were measured by ELISA method. Gills histopathological analysis showed that the highest damage in Karangkates reservoir dan Kali Jagir river is hyperplasia. Liver histopathological analysis in Karangkates reservoir showed normal hepatocytes meanwhile necrosis is highest damage found in Kali Jagir river. Metallothionein levels of fish in Karangkates reservoir for March, June, and September were 0.18 ng/mL; 0,18 ng/mL; and 0.2 ng/mL (gills fish); 0,18 ng/mL; 0,34 ng/mL; and 0,21 ng/mL (liver fish) respectively. Metallothionein levels of fish in Kali Jagir river for March, June, and September were 0,26 ng/mL; 0,18 ng/mL; and 0,18 ng/mL (gills fish); 0,2 ng/mL; 0,45 ng/mL; 0,19 ng/mL (liver fish) respectively. We conclude that the damage to the gills and liver of Barbonymus gonionotus was mostly found in Kali Jagir River and the highest metallothionein level of Barbonymus gonionotus also presented in Kali Jagir River.

Water quality and fish diversity in the Brantas River, East Java, Indonesia Alfiah Hayati; Nureka Tiantono; Muhamad Fadhil Mirza; Iman Dary Supriyadi Putra; Muhamad Maulana Abdizen; Antien Rekyan Seta; Binti Mar`atus Solikha; Muhamad Hilman Fu'adil; Trisnadi Widyaleksono Catur Putranto; Mochamad Affandi; Rosmanida Rosmanida
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 22 No 2 (2017): June 2017
Publisher : The East Java Biological Society

This research aimed to determine the water quality and fish diversity in Brantas river. Three station for sampling locations were in the upstream (one station on Karangkates reservoir) and downstream (two stations on the Surabaya and Jagir river). Water quality parameters were BOD, COD, DO, pH, temperature, and the levels of heavy metals (Pb, Cr, Cu, and Cd). Fish specimens was identified using fish identification book. The results of this research indicated that there was a difference in the water quality of upstream and downstream of the Brantas river. On the upstream, the source of pollution was mostly from fish catching, agriculture, and industrial activities. While on the downstream of the river it mostly dominated by industrial waste and domestic waste. The water quality in both stations had far exceeded the quality standards that was included in the polluted category. The concluded that Brantas river has been contaminated by waste (heavy metals), exceeded the water quality standard. The diversity of fish in the downstream (Surabaya river) has the highest diversity values, followed Jagir river and in the upstream (Karangkates reservoir) has the lowest value of diversity. Brantas river water was included in the category of moderate diversity.

PEMANFAATAN BIJI LABU KUNING (Cucurbita moschata) UNTUK PEMULIHAN MORFOLOGI DAN MOTILITAS SPERMATOZOA MENCIT (Mus Musculus) YANG TERPAPAR 2-METOKSIETANOL Alfiah Hayati; Nina Puspita; I.B. Rai Pidada
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 18 No 1 (2012): December 2012
Publisher : The East Java Biological Society

Research has done to investigate the effect of pumpkin seed extract on morphology and motility of the mices after given of 2-ME. Thirty male mices, 56–63 days with 23–27 g weight were used as experimental animals into 5 groups (2 groups is control and 3 groups is treatment), 6 mices each. The fi rst group is K0 which given 0.1 mL aquades by gavage for 40 days each day, and then K1 were given 0.05 mL 2-ME 200 mg/kg by intraperitoneally everyday within 5 days, and continued on the giving of aquadest. The next groups are treated groups which given 2-ME, in the fi rst 5 days, then continued to the givings of pumpkin seed extract by gavage. Cucurbita moschata seed extract were given in three doses: 0.5; 1.0; and 2.0 g/kg BB for 35 days. Sperm collected from cauda epididymis. Parameter of sperm quality are morphology and motility. The result shown that Cucurbita moschata seed extract doses 0.5–1.0 g/kg BB showed no significant effect and then doses 2.0 g/kg BB increased recovery process morphology and motility with signifi cant differences p < 0.05. The conclutions were Cucurbita moschata seed extract increased recovery process morphology and motility of the mices.

PENGARUH KADAR AIR TANAH TERHADAP BIOMASSA CACING TANAH Pontoscolex corethrurus Alfiah Hayati
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 1 No 1 (1995): June 1995
Publisher : The East Java Biological Society

This research was done in order to study of effect the soil humidity on earthworm Pontoscolex corethrurus biomass. 80 earthworms were placed in plastic pot (12.5 cm in diameter and 10 cm on deep) contained soil media (four individuals in each pot). Four treatment of humidity (45 %, 5 %, 55 % and 60%) with five replication was carried out up to six weeks. At the end of experiment animals were weighting. The difference between pre and post experimental body weigh was calculated, F test was used to test biomass differences among the four conditions of humidity. The result shown that the optimum biomass was in 50% soil humidity in the soil media.

HUBUNGAN KADAR MDA SPERMA DENGAN INTEGRITAS MEMBRAN SPERMATOZOA TIKUS (Rattus norvegicus) SETELAH PEMAPARAN 2–METHOXYETHANOL Alfiah Hayati; Soesanto Mangkoewidjojo; Aucky Hinting; Sukarti Moeljopawiro
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 11 No 2 (2006): June 2006
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/386

In the body, 2-methoxyethanol compound may be converted to MAA. MAA is a strong oxidant and may cause oxidation stress in spermatozoa. Oxidation stress is a disturbance on phosphorilation that increases ROS concentration, and it produces lipid peroxide in spermatozoa membrane resulted in high MDA concentration. One of indicator of spermatozoa membrane disturbances is a lack of spermatozoa membrane integrity. The main purpose of this research was to determine the relationship between MDA concentration in sperm and membrane integrity of spermatozoa in rats. The animal of treated groups (n = 40) were divide into 8 groups of 5 each. The rats were given subcutaneous injection with 0,2 ml of 200 mg/kg/day for 1 day (P1), 3 days (P2), 6 days/week (P3), and 12 days/two weeks (P4), respectively the control group was injected with physiological saline of the same volume. The concentration of MDA was measured by spectrophotometer and observing membrane integrity used HOS method to watch the spermatozoa response on hypoosmotic condition. The results of the research indicated that 2-ME caused the increasing in sperm MDA concentration and the decrease of spermatozoa membrane integrity. There was negative correlations between MDA concentration and spermatozoa membrane integrity.

EFEK AMFETAMIN TERHADAP ULTRASTRUKTUR SEL SPERMATOGENETIK TIKUS Alfiah Hayati
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 6 No 2 (2001): June 2001
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/497

The research was conducted to evaluate the effect of amphetamine as pshycostimulan drug on ultrastructure spermatogenic cells of male rats (Rattus norvegicus L.). Ultrastructure spermatogenic cells described from six male rats of Wistar strain. They were divided in to 2 groups (control and treatment group). Four mg/kg/days amphetamine were given sub-cutaninjection. In control to injection with physiologic saline. The last of the treatment the rat were killed after 100 days the amphetamine injection. The testis were released into fixative solution. Ultrastructure morphologic analysis of the testis were evaluate of quantitative to assess the effect of amphetamine on spermatogenic cells. Observation of spermatogenic cells (Spermatogonia, Spermatocyte, and Spermatyd cells) were done under the electrone microscope. The result of this experiment showed different morphologically from the amphetamine- treated group and their respective control. In the treatment groups, Spermatogonia, Spermtocyte, and spermayd cells less endoplasmic reticulum than normal spermatogenic cells (control). More vacuolization in membrane line of Spermatogoium cells and in cytoplasm of Spermatocyte, and Spermatic cells than normal spermatogenic cells (control).

Co-Authors Adelah Putri Milenia Sari Affandi, Moch Agoes Soegianto Agus Supriyanto Agustina, Widayati Almando Geraldi Amin, M. Hilman F. Analisa, Izza Analisa, Nur Izzah Ansori, Arif NM. Antien Rekyan Seta Aucky Hinting Aunurohim Aurira Thrisna Dwi Aprianti Azizaturrohmah Bayyinatul Muchtaromah Berlian Rustantina Binti Mar`atus Solikha Demircan, Turan Dewi, Firli RP. Dewi, Putri Alicia Duhita, Maharani R. Dwi Winarni Ely Nuril Fajriyah Erlyn Nurul Fauziah Erna Agustina Erna Agustina Fajriyah, Ely Nuril Farah Annisa Nurbani fatimah Fatimah Fatma Aulia Nadiyah Fatmi Indah Hati Fauziah, Erlyn Nurul Firdaus, Ana MK. Firli Rahmah Primula Dewi Gebby Agnessya Esa Oktavia Habibi, Soraya Hana Widyana Hanik Faizah I.B. Rai Pidada Idris, Muhammad F. Iman Dary Supriyadi Putra Inesavira Rindaputri Inne Ninda Resita Jamil, Arniza KM. Junairiah Junairiah Khaleyla, Firas Khusnita Giarti Ladiya Fitri Ma’rufah Laili Musyarofah Lestari, Lita I.D. Litazkiyyah, Litazkiyyah Manikya Pramudya Maya Safitri Maya Safitri Minarno, Eko B. Mochammad Affandi Muhamad Fadhil Mirza Muhamad Hilman Fu'adil Muhamad Maulana Abdizen MUHAMMAD FAHMI Muhammad Hilman Fu'adil Amin Mujahidin Ahmad Nabilla, Nithasya Nashrulloh, Allam Thoriq Nazil Dwi Rahayuningtyas Ni Wayan Sukma Antari Nimatuzahroh, Nimatuzahroh Nina Puspita Ninik Fadhillah Nita Yuliarini Nur Izzah Analisa Nurbani, Farah Annisa Nureka Tiantono Nuris Auliya Nurul Mahmudati Prilya Dewi Fitriasari Princessa Nandita Febrionny Resita, Inne Ninda Risanda Ulinnuha Rohmatika, Aulia U. Rosmanida Rosyidah, A'liyatur S. Sugiharto Salamun Salamun Salsabila, Syarifah Septiani, Adiibtia Setiyowati, Putri Ayu Ika Soesanto Mangkoewidjojo Soraya Habibi Sri Puji Astuti Wahyuningsih Sri Puji Astuti Wahyuningsih, Sri Puji Astuti Sri Sukmaningrum Sugiharto Suhestri Suryaningsih Sukarti Moeljopawiro Tri Nurhariyati Trisnadi Widyaleksono Catur Putranto Umi Nur Alfiah Ummi Zubaidah Virid Gibson, Virid Vuanghao Lim Vuanghao, Lim Widi Pangestu Wilujeng Widjiati Widjiati Widjiati Widjiati, Widjiati Yuli Winarsih

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