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BIOVALENTIA: Biological Research Journal

biov
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Published by Universitas Sriwijaya

ISSN : - EISSN : 24771392 DOI : https://doi.org/10.24233/biov

Core Subject : Health, Science, Agriculture, Social,

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Immunology & microbiology Medicine & Pharmacology

BIOVALENTIA: Biological Research Journal aims to review and publish the Biological research results from researchers. The range of articles which BIOVALENTIA: Biological Research Journal publishing is the research results in Biology science and applied Biology. Biology science is included Ecology, Biosystematics, Physiology, Developmental Biology, Microbiology, Genetics. Applied Biology is included Biotechnology, and the sciences derived from Biology science.

Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)

Articles 178 Documents

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PRIMER OPTIMIZATION AS GENETIC MARKER PRIMER BASED ON CYTOCHROME B GENE ON SUMATRAN ELEPHANT (Elephas maximus sumatranus) NON-INVASIVE SAMPLES Eko Agus Srihanto; Elly Lestari Rustiati; Priyambodo Priyambodo; Dian Neli Pratiwi; Alvin Wiwiet Susanto; Diah Esti Anggraini; Enny Saswiyanti
BIOVALENTIA: Biological Research Journal Vol. 8 No. 1 (2022)
Publisher : Biology Department, Faculty of Mathematics and Natural Sciences, Sriwijaya University

Sumatran elephant is declared as critically endangered by IUCN since 2011. In supporting conservation efforts on sumatran elephant in its natural habitat, molecular data collection of their population needs to be done. Genetic source material is mostly obtained in its natural habitat is its dung/feces. Study on cytochrome B gene as genetic marker from sumatran elephant fecal samples can be done to get additional genetic data as a form of conservation effort. Primer optimization as genetic marker based on cytochrome B gene has been carried out as an early study on sumatran elephant genetics. Primer optimization program based on cytochrome B genetic marker from sumatran elephant fecal samples was under Higher Education Applied Research funded by The Ministry of Research, Technology, and Higher Education. DNA was isolated from fecal samples of captive sumatran elephants in Elephant Training Center, Way Kambas National Park. Primer optimization was done via amplification by increasing certain temperature. Specificity test was done to ensure that the primer only recognized sumatran elephant DNA. The best result was obtained with the annealing temperature of 52oC

EXTRACTION AND PURITY DNA OF Culex spp MOSQUITO IN KEMELAK VILLAGE, BINDUNG LANGIT, OGAN KOMERING ULU Nuriessa Aputri, Farah; Hanum, Laila; Safitri, Ayu; Windusari, Yuanita
BIOVALENTIA: Biological Research Journal Vol. 7 No. 2 (2021)
Publisher : Biology Department, Faculty of Mathematics and Natural Sciences, Sriwijaya University

Culex spp are mosquito vectors that have a very wide distribution capability and are carriers of pathogens that can interfere with human and animal health. The wide distribution makes Culex spp a dangerous threat. DNA extraction is one of the important steps in obtaining genetic information and genetic analysis. Good quality DNA is used for activities such as the use of molecular markers, genome library creation, and sequencing. This study aims to determine the quality, concentration and purity of Culex spp mosquito DNA in Kemelak Bindung Langit Village, OKU Regency. It is hoped that the sample can be used for further research analysis on Mitochondria D-Loop Sequences in Culex spp mosquitoes. Quantitative measurement of DNA in the form of concentration and purity of DNA using Nanodrop Thermo cycle while qualitative DNA using electrophoresis technique. The results of the isolation of the mosquito genome DNA, obtained clear DNA bands without any degradation (smear) and the concentration results for the four samples ranged from 10-100 ng/ÂµL and the DNA purity was good, ranging from 1.8 to 2.00.

AMPHIBIAN DISTRIBUTION ON DEGRADATED HABITAT IN PASIR BINTANG AND MANDURIANG DISTRICT GUNUNG RAYA WILDLIFE SANCTUARY AREA OF SOUTH SUMATRA PROVINCE Aditya Yulistio; Zulkifli Dahlan; Zazili Hanafiah
BIOVALENTIA: Biological Research Journal Vol. 8 No. 1 (2022)
Publisher : Biology Department, Faculty of Mathematics and Natural Sciences, Sriwijaya University

Amphibian distribution on degradated habitat in Pasir Bintang and Manduriang districts of the Gunung Raya Wildlife Reserve, South Sumatra Province is caused by human activities, namely illegal logging and coffee plantations. Amphibians are one of the components that make up ecosystems that are very sensitive to environmental changes. This study aims to know the pattern of the amphibian species distribution on degradated habitat as a first step in conserving them. The method used is a line transect combination method with an encounter survey or Visual Encounter Survey (VES) with a time search of 3 hours in both aquatic and terrestrial habitats and the coordinates of the encounter are recorded. Trap by using a drift fence and pitfall traps at the affected location and also by using the interview method with the surrounding community and forest police. Determination of the distribution of amphibians is done by mapping the coordinates of the encounters that have been recorded using GPS with the help of the location of the coordinates on Google Earth into the amphibian distribution map. The results obtained are in the form of mapping the coordinates of amphibian encounters in the affected area. The conclusion of this study is the distribution of amphibian species in degradated habitat that have been encroached by illegal logging and coffee plantations are very few types and limited distribution due to habitat destruction and the small amount of food available.

SHORT COMMUNICATION: DAMAGE TO THE HISTOLOGICAL STRUCTURE OF MICE LIVER (Mus musculus) DUE TO EXCESSIVE CONSUMPTION OF SODIUM CHLORIDE Rizqi Nur Ardiansyah; Arum Setiawan; Yuanita Windusari
BIOVALENTIA: Biological Research Journal Vol. 8 No. 1 (2022)
Publisher : Biology Department, Faculty of Mathematics and Natural Sciences, Sriwijaya University

Salt or sodium chloride (NaCl) is used in everyday life mainly to give food taste. Salt is hepatotoxic, so excessive use of salt has an impact on the liver. The liver is one part of the organ that has an important role in the body's metabolism, especially in neutralizing toxins. The liver is composed of hepatocytes. There are 60% hepatocytes of the total cells in the liver. Hepatocytes are the main cells responsible for the central role of the liver in metabolism. Liver function becomes very vulnerable to damage because it continuously neutralizes toxins. Such damage can occur and affect the structure and function of the liver. This study was conducted to determine the structural abnormalities of hepatocytes that occur in the liver of mice (Mus musculus) due to excessive salt consumption. The method used in this study was a CRD (completely randomized design) method consisting of 1 control group and 3 treatment groups by sodium chloride (NaCl) with 6 replications. The parameter in this study was the level of hepatocyte damage in the liver of mice (Mus musculus). Hepatocyte damage that occurred was observed through histological preparations with Hematoxylin Eosin (HE) staining. The results of this study indicate that hepatocyte cell damage in the liver of mice (Mus musculus) increases due to continuous administration of excess sodium chloride (NaCl).

HIPPOCAMPUS CELL DISORDERS AND NEUROSENSORY TESTS IN MICE (Mus musculus) DUE TO INDUCTION OF EXCESS SODIUM CHLORIDE Cahyaningrum, Putri; Windusari, Yuanita; Setiawan, Arum
BIOVALENTIA: Biological Research Journal Vol. 7 No. 2 (2021)
Publisher : Biology Department, Faculty of Mathematics and Natural Sciences, Sriwijaya University

Salt or sodium chloride (NaCl) is an additive to give food a salty taste. The use of salt in everyday life is difficult to avoid. Salt has both good and bad effects on the body. The electrolyte content of salt can help launch metabolism in the body, whereas if the body contains too much salt it will cause heart attacks and hypertension. The dose of salt consumption that has been set by the government is 5g/day. This study was conducted to examine the effect of consuming excessive sodium chloride (NaCl) on the ability to smell and to what extent it damages cells in the hippocampus of mice (Mus musculus). The method of this study was CRD (completely randomized design) with 6 replications, 1 group control and 3 repetitions induced by sodium chloride (NaCl) is 260mg/gBB (P1), 520mg/gBB (P2), and 780mg/gBB (P3). The parameters of this study are neurosensory coordination in the form of olfactory response of mice (Mus musculus) to ammonia and cell disruption in the hippocampus (DG & CA) which were observed by histological preparations of Hematoxylin eosin (HE) staining. The results of this study indicate the presence of olfactory disorders in mice (Mus musculus) and cell death in the hippocampus also increased due to excessive sodium chloride (NaCl) induction. The more salt is consumed in daily life, it will disrupt the cells in the hippocampus.

THE APPLICATION OF DRY RUBBER LATEX AND VERMICOMPOST TO PLANT WATER CONSUMPTION AND THE GROWTH OF PADDY ON SWAMPY LAND Irmawati, Widya; Bernas, Siti Masreah; Bakri , Bakri
BIOVALENTIA: Biological Research Journal Vol. 7 No. 2 (2021)
Publisher : Biology Department, Faculty of Mathematics and Natural Sciences, Sriwijaya University

Swampy land is an alternative for rice plant cultivation where the amount of rainfall has an impact on the amount of inundation. In order to provide appropriate water for rice plants, especially during the dry season, an irrigation water system is required for its control.Alternative materials such as dry rubber latex and vermicom-post can be utilized to reduce water use and ensure that water is delivered as efficiently as possible. The goal of this study was to see how dried rubber latex on the soil surface and vermicompost in the soil affected rice plant water demand and growth. This research was conducted from November 2020 â€“ February 2021. The study used a factorial randomized block design (RAKF) with 2 treatments, covered dry rubber latex variations (K), K0 (0%), K1 (25%), K2 (50%), K3 (75%), and vermicompos (V), V0(0 g), V1(58.82 g), V2(117.64 g), V3 (176.46 g)with 3 rep-licat. So that the total treatmentsare 48 treatments.The results showed that the application of dry rubber latex and vermicompost significantly affected the number of leaves and the number of tillers. The K3 treatment (75%) was the best because it had the smallest evapotranspiration rate with an average of 1.2 mm day-1. The K3 treatment (75%) had the smallest average water requirement of 1.6 mm day-1 with an average number of 37.07 leaves. The high yield of K3 treatment was due to the wider the area covered by 75% (K3), the higher the water content, nutrients, and the lower the soil temperature. Closure using dry rubber latex can reduce sunlight so that the process of water loss is lower, and the available nutrients are higher.

THE EFFECT OF CULTURE MEDIA TYPE AND PLANT GROWTH REGULATORS ON CALLUS INDUCTION OF OIL PALM (Elaeis guineensis Jacq) PISIFERA TYPE Sulaksono, Gogoh; Hasmeda, Mery; Hanum, Laila; Wendra, Fahmi; Santika, Baitha; Asmono, Dwi
BIOVALENTIA: Biological Research Journal Vol. 7 No. 2 (2021)
Publisher : Biology Department, Faculty of Mathematics and Natural Sciences, Sriwijaya University

Oil palm has a high economic value since it is one of the highest vegetable oil-producing plants compared to other oil-producing plants. The provision of good planting material for oil palm plantations is one of the determining factors to increase productivity. The parent Pisifera type of oil palm is important to develop the high-yielding oil palm Tenera varieties. The provision of oil palm material could be done through generative system with seeds and also could be done through vegetative approaches using tissue culture techniques. It aims to get plants that are genetically the same as their parents (true to type). The purpose of this study was to see the effect of different type of tissue culture media and plant growth regulators (PGRâ€™s) on the callus induction stage of Pisifera type oil palm. The results show that the treatment using Murasige and Skoog (MS) culture media is able to give a better effect when compared to the use of Eeuwens and Blake (Y3) basic media. While the use of PGR treatment (H6 = NAA 6 mg/l + 2.4-D 0.5 mg/l) showed a callus growth percentage of 76%, which was better than (H1 = Kin 0.1 mg/l + 2.4-D 100 mg/l) by 42%.

HISTOLOGY TEST OF TILAPIA (Oreochromis niloticus) ON ADSORBENT PERFORMANCE RESULTS IN LIQUID TREATMENT OF INDUSTRY PULP AND PAPER WASTE Susila Arita Rachman; Devi Kristianti; Yuanita Windusari
BIOVALENTIA: Biological Research Journal Vol. 8 No. 1 (2022)
Publisher : Biology Department, Faculty of Mathematics and Natural Sciences, Sriwijaya University

Pulp and paper industry fly ash is categorized as B3 waste, namely hazardous and toxic waste. Liquid waste produced by the pulp and paper industry contains pollutants that are toxic and can cause pathological and histopathological changes in important tissues such as the gills of fish that live in the waters around the disposal. The purpose of this study was to analyze the histology of tilapia on the performance results of fly ash adsorbents in the management of liquid waste in the pulp and paper industry. The research method has 6 treatments, namely the ratio of fly ash to pulp and paper liquid waste (20 g; 1 L) namely P0: 100 % well water control, P1: lowest liquid waste 5 % + 9.5 % well water, P2: 5 % liquid waste fly ash adsorbent + 95 % well water, P3: 6.25 % liquid waste fly ash adsorbent + 93.75 % well water, P4: 7.5 % liquid waste fly ash adsorbent + 92.5 L of well water, P5: 8.75 % fly ash adsorbent + 91.25 % well water, P6: 10 % fly ash adsorbent + 9 % well water. Water quality measurements such as temperature, NH3, pH and DO were carried out. Observation of gill histology used microtechnical method which includes deparaffinization and histological staining (HE). Analysis was carried out on changes in cell morphology in gill tissue. The results showed that the morphology of gill cells in liquid waste pulp and paper 5 % found gill damage in the form of congestion, secondary lamella fusion, secondary filament rupture and goblet proliferation occurred in the treatment of wastewater treatment while Fly ash adsorbent 5 % damaged gills in the form of congestion and fusion of secondary lamellae. From the results of the Fly ash functions can reduce damage to body organs in fish, especially on the gills so that it can reduce the number of deaths in fish compared to treatment without the use of fly ash.

GERMINATION OF PORANG (Amorphophalus muelleri) FROM DIFFERENT BULBIL TO VARIOUS PLANTING MEDIA Nurjannah, Hegar; Umar Harun, Muhammad; Sodikin, Erizal
BIOVALENTIA: Biological Research Journal Vol. 7 No. 2 (2021)
Publisher : Biology Department, Faculty of Mathematics and Natural Sciences, Sriwijaya University

Porang plant is atubers that havevarious benefits. Porang cultivation needs intensive management through cultivation efforts which of course must be supported by the availability of seeds. Therefore, it is necessary to procure porang seeds from vegetative (tubers) and generative (stem bulbil and branch bulbil). This study aims to obtain the best growing media for porang germination from various types of bulbil. The method used in this study was a factorial randomized block design. The way of working in this research is: Preparation of Seedling Media, Preparation of planting material (Bulbil), Seeding, Maintenance (including watering and weeding). The results showed that there was no significant interaction between the planting medium and the type of bulbil, the media had no significant effect on all observed variables, while the bulbil had a significant effect on the percentage of germination growth, stem height, and number of leaves. The best type of bulbil for breeding is stem bulbil which is superior to the variables of plumule emergence time, petiole length, stem height, and number of leaves compared to branch bulbil. Meanwhile, the best growing media in porang nurseries was a mixture of 50% husk charcoal + 50% sawdust on the variables when the plumule appeared, the percentage of germination, petiole length and stem height. The appearance of bulbil from stems on a mixture of 50% husk charcoal + 50% sawdust media, namely the time of emergence of plumules (5.61 days), percentage of sprouting (86.67%), petiole length (8.72 cm), height stems (31.11 cm) and number of leaves (7.72 strands).

SYNERGISM BETWEEN RHIZOSPHERE BACTERIA ISOLATES FROM Scleria sp., Clidemia sp., AND Panicum sp. TO INCREASE THE EFFECTIVENESS OF MIXED CULTURES IN HYDROCARBON BIODEGRADATION Hardestyariki, Dwi; Yudono, Bambang; Munawar, Munawar
BIOVALENTIA: Biological Research Journal Vol. 7 No. 2 (2021)
Publisher : Biology Department, Faculty of Mathematics and Natural Sciences, Sriwijaya University

The purpose of this research is to obtain hydrocarbon degrading bacteria that work synergistically in a consortium. Consortium microorganisms is mixture of microbial populations in the form of communities that have mutualistic relationships and doesnâ€™t inhibition the growth of other microbes. In this study, isolates were obtained from the rhizosphere of soil contaminated with petroleum. The isolates obtained were tested for synergism to determine the relationship between bacterial isolates. Synergism testing was carried out using the spread plate method on agar media. The results of this study showed that isolate number one showed antagonistic properties to other bacterial isolates by forming a clear zone around the disc paper. A total of eight bacterial isolates showed the greatest percentage of synergism, namely â‰¥ 80% so that the eight rhizosphere bacterial isolates could be used as materials for mixed culture.

Page 10 of 18 | Total Record : 178

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Home Page

OAI Link

Editorial Team

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Reviewer

Google Scholar

Contact Name Enggar Patriono

Contact Email epatriono@unsri.ac.id

Phone +62711 580306

Journal Mail Official biovalentia@unsri.ac.id

Editorial Address Biology Department, Faculty of Mathematics and Natural Sciences, Sriwijaya University Palembang-Prabumulih Road KM 32 Indralaya, Ogan Ilir, South Sumatera 30662 Indonesia

Location Kab. ogan ilir, Sumatera selatan INDONESIA

Abstract

Abstract

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Abstract

Contact Name
Enggar Patriono

Contact Email
epatriono@unsri.ac.id

Phone
+62711 580306

Journal Mail Official
biovalentia@unsri.ac.id

Editorial Address
Biology Department, Faculty of Mathematics and Natural Sciences, Sriwijaya University Palembang-Prabumulih Road KM 32 Indralaya, Ogan Ilir, South Sumatera 30662 Indonesia

Location
Kab. ogan ilir,

Sumatera selatan

INDONESIA