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Stigma : Jurnal Matematika dan Ilmu Pengetahuan Alam Unipa
Published by Universitas PGRI Adi Buana Surabaya
ISSN : 14121840     EISSN : 26219093     DOI : https://doi.org/10.36456/stigma.15.01
Core Subject : Education,
Mathematics
Jurnal STIGMA adalah jurnal ilmiah Biologi dan Biologi Terapan yang memuat artikel-artikel ilmiah. Jurnal STIGMA diterbitkan oleh Program Studi Biologi-Fakultas Matematika dan Ilmu Pengetahuan Alam - Universitas PGRI Adi Buana Surabaya. Terbit dua kali dalam satu tahun (April-Juli dan September-Desember), Jurnal ini memfasilitasi penelitian dengan tema Biologi Umum, Biologi Terapan, Bioteknologi, Aplikasi Ilmu Biologi.
Arjuna Subject : Umum - Umum
Articles 7 Documents
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Search results for , issue "Vol 18 No 01 (2025)" : 7 Documents clear
STUDI LITERATUR: STRUKTUR GENOME DAN IDENTIFIKASI GEN YANG BERPERAN DALAM KETAHANAN DEINOCOCCUS RADIODURANS TERHADAP RADIASI Anisa Oktobianti , Andi; Jamaluddin, Nurbina Septiani; Hala, Yusminah
STIGMA: Jurnal Matematika dan Ilmu Pengetahuan Alam Unipa Vol 18 No 01 (2025)
Publisher : Universitas PGRI Adi Buana Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36456/stigma.18.02.10135.%p

Abstract

Deinococcus radiodurans is an extremophilic bacterium with exceptional resistance to ionizing radiation, oxidative stress, and extreme environmental conditions. This resistance is supported by a unique multipartite genome structure, efficient DNA repair mechanisms, and complex global regulation. Key genes such as recA, ssb, pprA, and irrE play crucial roles in DNA repair and cellular protection. The bacterium can fully reconstruct its genome after severe radiation-induced damage, making it an ideal model for studying DNA repair and stress response mechanisms. Due to its remarkable resilience, D. radiodurans hold great potential for various applications, including radioactive waste bioremediation, radiation protection in medical fields, and advancements in biotechnology and genetic engineering. Further research is necessary to explore its molecular mechanisms and technological applications in industry, healthcare, and environmental sectors.
Thermus aquaticus: Keunikan Genetik dan Perannya dalam Revolusi PCR melalui Taq Polymerase Tenri Ampa Nurfitria Papada, Andi; Astuti S, Dewi; Hala, Yusminah
STIGMA: Jurnal Matematika dan Ilmu Pengetahuan Alam Unipa Vol 18 No 01 (2025)
Publisher : Universitas PGRI Adi Buana Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36456/stigma.18.02.10163.14-21

Abstract

Thermus aquaticus is a thermophilic bacterium known for its ability to produce thermostable enzymes, such as Taq polymerase, which plays an important role in the Polymerase Chain Reaction (PCR) technique. This study aims to examine the characteristics, ecology, and potential applications of thermostable enzymes produced by this bacterium in various industrial and biotechnological fields. The study was conducted through a literature review using a search method in scientific databases such as PubMed, Scopus, and Google Scholar. The results of the study showed that T. aquaticus has an optimal growth temperature between 70-75°C and is found in extreme environments such as hot springs and hydrothermal systems. Its characteristics, which include strong cell walls, unique membrane lipid content, and thermostable enzymes, allow this bacterium to survive in high temperature conditions. The Taq polymerase enzyme isolated from this bacterium has revolutionized the field of biotechnology, especially in PCR which is used for DNA amplification in genetics, medicine, and forensics research. In addition to PCR, thermostable enzymes from T. aquaticus have wide applications in the food, pharmaceutical, and bioremediation industries. Its existence in the ecosystem shows an important role in the biogeochemical cycle and its interaction with other microorganisms in extreme environments. This finding confirms that the exploration of extremophile microorganisms, including T. aquaticus, still has great potential in technological innovation and the development of biotechnology-based industries.
Antagonistic Activity of Pseudomonas fluorescens Against Curvularia sp. Causing Leaf Spot Disease in Maize Vivin Andriani; Purity Sabila Ajiningrum; Riza Putri Hanifah
STIGMA: Jurnal Matematika dan Ilmu Pengetahuan Alam Unipa Vol 18 No 01 (2025)
Publisher : Universitas PGRI Adi Buana Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36456/stigma.18.01.10320.%p

Abstract

Maize (Zea mays L.) is an important agricultural commodity in Indonesia, yet its production is often hindered by pathogenic infections, notably Curvularia sp., the causal agent of leaf spot disease. This study aimed to evaluate the antagonistic activity of Pseudomonas fluorescens against Curvularia sp. under in vitro conditions. The pathogenic fungus was isolated from infected maize leaves, while P. fluorescens was isolated from soil samples and identified through biochemical tests and pigment production on King’s B medium. Observations revealed that P. fluorescens produces fluorescent pigments, is an obligate saprophyte, and does not induce soft rot in potato tissue. Antagonism assays showed that the growth of Curvularia sp. was inhibited in the presence of P. fluorescens, as evidenced by fungal hyphae growing away from the bacterial colony. This inhibitory effect is likely due to antifungal secondary metabolites produced by P. fluorescens, including antibiotics, hydrogen cyanide, siderophores, and extracellular enzymes such as chitinase and cellulase, which degrade fungal cell walls. These findings suggest that P. fluorescens holds promise as a biocontrol agent for managing leaf spot disease in maize.   Keywords: Zea mays, Pseudomonas fluorescens, Curvularia sp., antagonism, leaf spot disease
EFEK PEMBERIAN INFUSA KOMBINASI TEMU IRENG, TEMULAWAK, KENCUR DAN JAHE TERHADAP BERAT MENCIT GALUR SWISS-WEBSTER Indira Pipit Miranti; Arinda Nur Cahyani; Eko Hidayaturohman; Ninstar Fathina Rifa
STIGMA: Jurnal Matematika dan Ilmu Pengetahuan Alam Unipa Vol 18 No 01 (2025)
Publisher : Universitas PGRI Adi Buana Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36456/stigma.8.01.10354.%p

Abstract

Signs that children have difficulty eating and will then trigger malnutrition are very limited nutritional intake and a reduced appetite. Temu ireng, curcuma, sand ginger and ginger rhizomes contain essential oils, curcumin, xanthorizol, gingerol and flavonoids in each plant which can increase body weight and appetite. The purpose of this study was to determine the effect and optimal frequency of infusion combination on body weight and feed consumption of mice. The research was conducted in the Pharmacology Laboratory of STIKes Ibnu Sina Ajibarang. This type of research is true experimental with pre-post-test control design method. 28 male mice were divided into four groups. Group P0 (aquadest), group P1 (½ x dose), group P2 (1x dose) and group P3 (1 ½ x dose). All treatment groups were acclimatized for 7 days, then treated according to the predetermined dose, then observed body weight gain and feed consumption for 28 days. Data analysis was conducted using paired t-test, One Way ANOVA test, and Post-hoc LSD test. The results showed that there was a significant effect on body weight gain in group P2 (p=0.000) and feed consumption in all groups in the first, third and fourth weeks. (p<0,05).   Keywords; Effect, Body Weight, Infusa, Rhizome.
Pemberian Probiotik EM-4 (Effective Mikroorganism 4) Untuk Menumbuhkan Klekap Sebagai Pakan Alami Guna Meningkatkan Pertumbuhan Dan Survival Rate Benih Ikan Bandeng (Chanos sp.) Rifka Ayu Layyinah; Pungky Slamet Wisnu Kusuma; Dyah Hariani
STIGMA: Jurnal Matematika dan Ilmu Pengetahuan Alam Unipa Vol 18 No 01 (2025)
Publisher : Universitas PGRI Adi Buana Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36456/stigma.8.01.10420.%p

Abstract

The study aimed to determine the effect of EM-4 probiotic administration on natural klekap feed with different doses on the growth and survival of milkfish (Chanos sp) seeds. The test fish of milkfish (Chanos sp) with a length of 2 cm and a weight of 2 grams came from a milkfish seed cultivation reservoir in Mengarih Village, Manyar District, Gresik Regency, randomly stocked into 12 ponds with a fish density of 30 fish/pond and tested for 30 days. This study used a Complete Random Design with 4 treatments and 3 replicates. The treatment given was the difference in the dose of probiotics added in klekap natural feed, which consisted of treatment without the addition of probiotics, treatment with the addition of probiotics as much as 15 ml/L, 25 ml/L, 35 ml/L. Data were analyzed using Variety Analysis (ANOVA) with a level of (P<0.05). Test parameters included absolute weight growth rate (LPBM), absolute length growth (PPM), and survival rate (SR). The results showed that the administration of EM-4 on different natural klekap feeds had a real effect (P<0.05) on LPBM, PPM, and SR. The best values were for LPBM (5.73±0.14 grams), PPM (10.33±0.04 mm). The average success rate (SR) during the experiment was approximately 88.75%.   Keywords: Milkfish Seed (Chanos Sp), Survival, Klekap, Probiotic EM-4, Growth
Diversity And Abundance Of Macrozoobentos And Pisces In The Mangrove Area Of Gresik District, Kebomas And Manyar Gresik Regency Vidia Kristanti; Diah Karunia Binawati
STIGMA: Jurnal Matematika dan Ilmu Pengetahuan Alam Unipa Vol 18 No 01 (2025)
Publisher : Universitas PGRI Adi Buana Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36456/stigma.18.01.10556.%p

Abstract

Mangrove ecosystems play an important role in the life cycle of various types of fish, shrimp and crabs. Because the mangrove environment provides protection and nutrients in the form of organic matter that is included in the food chain. Some of the fauna that are commonly found in the Indonesian mangrove ecosystem area are fauna from the gastropod, crustacean, bivalve, polychaeta, and pisces classes. One of the biota that can be used as biological parameters in determining the conditions of a body of water is macrozoobentos. As organisms living in waters, macrozoobentos are very sensitive to changes in the quality of the water in which they live so that it will affect their composition and abundance. This research method is quantitative descriptive research by taking data in a survey. It uses a 1x1m measuring plot 100m long perpendicular to the coastline. The measurement method used to determine the environmental conditions of mangroves is the transect line method and sample plots (Tran-sect Line Plot). The diversity index value at research locations I and II is in the medium category, while in location III it is in the high category. Of the three stations, the uniformity index is stable close to 1. Result The dominance index value is that there is no type that dominates a body of water, this means that all individuals at the observation station have the same opportunity and maximally make the most of the resources in those waters. The pH results of water at station II and station III did not differ significantly, while at station I it differed significantly when compared to the results at stations II and III. The salinity results at station III differ significantly from station I and II because the location of the mangrove is the location of a sea estuary that makes the salt content of brackish water mixed with fresh water.    Keywords ; Mangroves, Macrozoobentos, Diversity, Uniformity, Dominance, Abundance, Abiotic Factors
Effect Of NAA And Kinetin Administration On Callus Induction In Vanilla Plant Stem Explants (Vanilla planifolia Andrews) Dhavin, Muhammad; Nurokhman, Amin; Ummi Hiras Habisukan; Yustina Hapida; Anggun Wicaksono; Weni Lestari; Arif Yachya
STIGMA: Jurnal Matematika dan Ilmu Pengetahuan Alam Unipa Vol 18 No 01 (2025)
Publisher : Universitas PGRI Adi Buana Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36456/stigma.18.01.10574.%p

Abstract

Vanilla (Vanilla planifolia Andrews) is a plantation plant that is used for its fruit. Indonesia is one of the countries that exports vanilla and has climate factors that support increasing vanilla production to meet global demand, One of the efforts that can be made in vanilla propagation is through plant tissue culture. This research aims to induce calluses. This study used a Random Design using stem explants and several different hormone concentrations, namely: 0.0 ppm NAA and 0.0 ppm Kinetin, 1.0 ppm NAA and 0.5 ppm Kinetin, 1.0 ppm NAA and 1.0 ppm Kinetin, 2.0 ppm NAA and 0.5 ppm Kinetin, 2.0 ppm NAA and 1.0 ppm Kinetin, 2.0 ppm NAA and 2.0 ppm Kinetin. There was an effect on the administration of NAA and Kinetin on callus induction in the explants of the vanilla plant (Vanilla planifolia Andrews). It is based on the Anova one-way Hypothesis Test with a significance value of 0.000 (Asymp. Sig <0.05) then Ho was rejected and H1 was accepted. The optimal concentration to induce callus in stem explants is the administration of 1.0 ppm NAA + 0.5 ppm Kinetin with an average callus growth rate of 7 HST and a 100% callus growth percentage of explants.   Keywords: Vanilla, induction, callus, tissue culture, hormones

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