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JKKI : Jurnal Kedokteran dan Kesehatan Indonesia
Published by Universitas Islam Indonesia
ISSN : 20854145     EISSN : 25272950     DOI : 10.208885/JKKI.Vol10.Iss3.art5
Core Subject : Health,
Medicine & Pharmacology Public Health
JKKI: Jurnal Kedokteran dan Kesehatan Indonesia is a peer-reviewed journal in the field of medical and health sciences. This journal is designed as a place of dissemination of information and scientific knowledge, which publishes three times a year. It publishes original article, article review, and case report. These comprise of biomedical sciences, clinical medicine, public health sciences, and medical science education.
Arjuna Subject : Kedokteran - Kedokteran Keluarga
Articles 468 Documents
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Effect of concentration veriations exstract etanol salam leaves (eugenia polyantha wight) on risk free radicals dpph (1,1-diphenyl-2-picrylhydrazyl) Any Guntarti; Nurdiansyah Nurdiansyah
JKKI : Jurnal Kedokteran dan Kesehatan Indonesia JKKI, Vol 10, No 1, (2019)
Publisher : Faculty of Medicine, Universitas Islam Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20885/JKKI.Vol10.Iss1.art10

Abstract

Background: Salam leaves (Eugenia polyantha Wight) contains antioxidant compounds, flavonoids and polyphenols. These compounds are reported to inhibit oxidation activity by binding with free radicals. The unlimited amount of free radicals in the body will increase the risk of contracting degenerative diseases.Objective: The purpose of this study was to identify compounds from the ethanol extract of salam leaves and to test the antioxidant activity of its compounds using DPPH method and also determine the IC50 (Inhibition Concentration 50) of between the ethanol extract of salam leaves and quercetin standard. Methods: Ethanol concentration extract of salam leaves were obtained by maceration method with the ethanol solution. The compounds were identified by phytochemical screening. The concentration used for the extraction of salam leaves were 30%, 40% and 50%. The variations of each extract concentration were 50, 75, 100, 125 and 150 mg/mL. The variations of standard quercetin concentration were 1, 2, 3, 4 and 5 mg/mL. Antioxidant activity was tested by visible spectrophotometry using the DPPH method. The antioxidant activity is represented as the per cent of free radical scavenging and IC50. Results: The 30%, 40% and 50% ethanol extract of salam leaves contained flavonoid and polyphenol compounds, and also had free radical binding activity. The average value of IC50 quercetin, 30%, 40% and 50% ethanolic extract of salam leaves were 1.68, 102.48, 149.88 and 197.19 mg/mL.Conclusion: Statistical analysis showed that the antioxidant activity between quercetin, 30%, 40% and 50% ethanol extract of salam leaves were significantly different (P=95%). 
Total phenolic content and antioxidant activities of binahong (Anredera cordifolia.) Hari Susanti
JKKI : Jurnal Kedokteran dan Kesehatan Indonesia JKKI, Vol 10, No 2, (2019)
Publisher : Faculty of Medicine, Universitas Islam Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20885/10.20885/JKKI.Vol10.Iss2.art9

Abstract

Background: Binahong plants are reportedly containing polyphenols, flavonoids, and steroid compounds. The content of polyphenols and flavonoids plays an important role as antioxidants. Research using the method of FRAP (Ferric Reducing Ability of Power) indicates that the antioxidant content of the binahongleaves extract is 3.68 mmol/100 g in dried leaves. This study offers new proof of antioxidant activity of all the aerial part of binahong.Objective: This research aims to determine the total phenolic content from binahong extract and its ability as free radical scavenger using DPPH method.Methods: The plant used in this research is every part of plants on the ground. The binahong extract is obtained by a maceration method with various solvents (hexane, chloroform, and methanol). Total phenolic content in the extract is spectrophotometrically determined with the Folin Ciocalteu reagent. Antioxidant activity assay is performed in vitro by the method of DPPH, with Gallic acid as a comparator. ES50 parameter is determined from the linear regression equation between the concentration of extracts and % scavenging.Results: The results showed that the total phenolic content of hexane, chloroform and methanol extract of Binahong was 8.54 ± 0.49 GAE mg/g, 17.30 ± 0.47 GAE mg/g and 32.5 ± 1.11 GAE mg/g. Hexane extract, chloroform extract and methanol extract of Binahong have a free radical scavenger activity. The value of ES50 extracts of hexane, chloroform, methanol, and successive acid errors is 58.601 ± 2.533 µg/ml, 446.219 ± 2.268 µg/ml, 237.683 ± 13.373 µg/ml and 2.058 ± 0.002 µg/ml.Conclusion: The capability of the three of binahong extract as a free radicals DPPH scavenger were weaker than gallic acid.Background: Binahong plants are reportedly containing polyphenols, flavonoids, and steroid compounds. The content of polyphenols and flavonoids plays an important role as antioxidants. Research using the method of FRAP (Ferric Reducing Ability of Power) indicates that the antioxidant content of the binahongleaves extract is 3.68 mmol/100 g in dried leaves. This study offers new proof of antioxidant activity of all the aerial part of binahong.Objective: This research aims to determine the total phenolic content from binahong extract and its ability as free radical scavenger using DPPH method.Methods: The plant used in this research is every part of plants on the ground. The binahong extract is obtained by a maceration method with various solvents (hexane, chloroform, and methanol). Total phenolic content in the extract is spectrophotometrically determined with the Folin Ciocalteu reagent. Antioxidant activity assay is performed in vitro by the method of DPPH, with Gallic acid as a comparator. ES50 parameter is determined from the linear regression equation between the concentration of extracts and % scavenging.Results: The results showed that the total phenolic content of hexane, chloroform and methanol extract of Binahong was 8.54 ± 0.49 GAE mg/g, 17.30 ± 0.47 GAE mg/g and 32.5 ± 1.11 GAE mg/g. Hexane extract, chloroform extract and methanol extract of Binahong have a free radical scavenger activity. The value of ES50 extracts of hexane, chloroform, methanol, and successive acid errors is 58.601 ± 2.533 µg/ml, 446.219 ± 2.268 µg/ml, 237.683 ± 13.373 µg/ml and 2.058 ± 0.002 µg/ml.Conclusion: The capability of the three of binahong extract as a free radicals DPPH scavenger were weaker than gallic acid.
Kidney tubular injury of rats caused by unripe green betel nuts (Areca catechu) Ave Olivia Rahman; Anati Purwakanthi
JKKI : Jurnal Kedokteran dan Kesehatan Indonesia JKKI, Vol 11, No 1, (2020)
Publisher : Faculty of Medicine, Universitas Islam Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20885/JKKI.Vol11.Iss1.art5

Abstract

Background: Unripe green betel nuts (Areca catechu) are usually consumed as herbal medicines by some inhabitants in several regions of Indonesia. It is considered that the nuts contain Arecoline hydrobromide that can cause damages in a kidney tubule, although it needs further studies about effects of the nuts.Objective: This study aims to determine the effects of the unripe green betel nuts on kidney tissues of rats in a long-term treatment.Methods: Twenty rats used in this study were Sprague-Dawley strain, male, 2-3 months old, and 150-200 gram of weight. They were randomly divided into four groups. Group I was control group, given distilled water once a day; group II, III, and IV were served by juice of the unripe green betel nuts with a dose of 250 mg/kgBW, 1,000 mg/kgBW, and 10,000 mg/kgBW, respectively. Histopathology examination by staining of Haematoxylin Eosin was conducted by an anatomic pathologist using blind methods including glomerulus, tubulus, and interstitial tissues.Result: The tested kidney tissues showed mild interstitial congestion, tubular cast, and tubular degeneration in groups that received unripe green betel nuts. There were no glomerulus abnormality and interstitial inflammation. The highest percentage of rats suffering injury in their kidney was the group IV, with p value <0.05 when compared to the control group.Conclusion: Long-term consumption of unripe green betel nuts could cause kidney tubular injury.
Relationship between knowledge of acute respiratory tract infection and use of face masks in motorcyle online drivers Ahmad Saepudin; Eveline Margo
JKKI : Jurnal Kedokteran dan Kesehatan Indonesia JKKI, Vol 12, No 1, (2021)
Publisher : Faculty of Medicine, Universitas Islam Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20885/JKKI.Vol12.Iss1.art3

Abstract

Background: Data in 2013 states that the prevalence of acute respiratory infections (ARI) is 25%. One of the factors that cause ISPA is air pollution. This factor is one of the risk factors for motorcycle taxi drivers who are always exposed to air pollution. Knowledge of ARI that is sufficient to influence the use of masks can help prevent ARI from occurring.Objective: This study aims to determine the relationship between knowledge of ARI and the behaviour of using masks among online motorcycle drivers.Methods: This study was an analytical observational with a cross-sectional design attended by 139 online motor cycle drivers around Grogol, Petamburan, West Jakarta, from July to December 2017. Data collection was done by filling out a questionnaire that includes demographic data, the use of masks, and knowledge of ARI. Data analysis used Fisher's test with significance level p<0,05.Results: A total of 139 respondents were collected, consisting of 131 (94.2%) men and 8 (5.8%) women. The average age of > 30 years old is 77 (55.4%) people. Most respondents' education level was SMA/SMK/SLTA as many as 111 (79.9%) people. From the results of Fisher's test, it was found that the probability value p=0.362 was greater than the significance value (0.05).Conclusion: There is no significant relationship between the level of knowledge about ARI and the use of masks among online motorcycle drivers.
FRAKSI ETIL ASETAT SPONS Stylissa carteri MENURUNKAN VIABILITAS SEL KANKER PAYUDARA TIPE HER2+ RESISTEN TRASTUZUMAB Julia Nur Jannah; Pandji Irani Fianza; Tenny Putri; Muhammad Hasan Bashari
JKKI : Jurnal Kedokteran dan Kesehatan Indonesia JKKI, Vol 10, No 3, (2019)
Publisher : Faculty of Medicine, Universitas Islam Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20885/JKKI.Vol10.Iss3.art6

Abstract

Background: HER2+ breast cancer is a very aggressive type of breast cancer. Although trastuzumab, specifically targeted for HER2, has been used for breast cancer treatments, some patients become resistant to trastuzumab. A marine sponge is one of the potential sources of anticancer agents. One of marine sponges commonly found in Indonesia is Stylissa carteri, but it has not been explored extensively.Objective: This study aimed to identify cytotoxic effects of the ethyl acetate fraction of Stylissa carteri on HCC-1954, HER2+ trastuzumab-resistant breast cancer cells, by assessing cell viability and determining IC50 value.Methods: This study was an experimental in vitro study conducted in The Cell Culture Laboratory, Faculty of Medicine Universitas Padjadjaran on February 2018 to June 2018. The Stylissa carteri was collected from Pramuka Island, Kepulauan Seribu National Park Jakarta. HCC-1954 cells were treated by serial concentration of fractions and were incubated for 72 hours. Cell viability were observed under a microscope and analysed with MTT assay. The IC50 value was also determined by using four parametric logistic regression (4PL) method by Sigmaplot version 12.0. Result: Data of this study showed descent of cell viability significantly when exposing the ethyl acetate fraction of Stylissa carteri. There was a decrease of 49% cell viability in 10 µg/ml of the ethyl acetate fraction of Stylissa carteri. The estimated IC50 value was 9.25 µg/ml.Conclusion: This result indicated that the ethyl acetate fraction of Stylissa carteri has cytotoxic effects on HER2+ trastuzumab-resistant breast cancer cells.
Adopting Mass Thalassemia Prevention Program in Indonesia: a Proposal Lantip Rujito; Joko Mulyanto
JKKI : Jurnal Kedokteran dan Kesehatan Indonesia JKKI, Vol 10, No 1, (2019)
Publisher : Faculty of Medicine, Universitas Islam Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20885/JKKI.Vol10.Iss1.art1

Abstract

No Abstract
An exploratory study on the dimensions of spiritual care Henie Kurniawati; Sofia Retnowati; Bagus Riyono; Widyawati Widyawati
JKKI : Jurnal Kedokteran dan Kesehatan Indonesia JKKI, Vol 10, No 2, (2019)
Publisher : Faculty of Medicine, Universitas Islam Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20885/10.20885/JKKI.Vol10.Iss2.art5

Abstract

Background: Spiritual care is one of the important services. The dimensions of spiritual care have not been fully identified clearly. Therefore it is necessary to explore the dimensions of spiritual care.Objective: The aim of this study is to explore spiritual care.Methods: Qualitative research methods were used in order to obtain data through observation techniques, in-depth interviews, and focus group discussions (FGD). The research was conducted within several number of hospitals in Central Java, Indonesia. The samples of this research were 57 nurses with given criteria as following: has a minimum education of associate’s degree in the nursery and a work experience minimum of 2 years in a hospital. Obtained data were, was analyzed with constant comparative and content analysis.Result: The exploration of spiritual care is depicted by hospital nurses as personal belief towards spirituality that can be experienced through spiritual comfort, which is manifested through routine worships, prayers, and intense presence as spiritual implementations and support.Conclusion: This explorative study concludes that there are dimensions in the spiritual care of hospital nurses: personal belief, spiritual knowledge, spiritual comfort, spiritual implementation, and spiritual support. The implications may be able to bring benefit in its application toward the government, hospitals, nurses, patients, and their families, as well as the society.
Microbial approach of epitope tagged MFE-23 single fragment antibodies production Razmaeda Sarastry
JKKI : Jurnal Kedokteran dan Kesehatan Indonesia JKKI, Vol 11, No 3, (2020)
Publisher : Faculty of Medicine, Universitas Islam Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20885/JKKI.Vol11.Iss3.art3

Abstract

Background: Antibodies have been investigated for future clinical application in cancer management. An antibody, MFE-23 scFv is known for its ability to bind Carcinoembryonic Antigen (CEA). Different from a full length antibody, single-chain variable fragments (scFvs) are recombinant antibodies in which single polypeptide is engineered to replace variable regions encoding antigen-binding domain. In vitro production of single chain fragment antibodies may use E. coli microorganism for its ability to self-replicating a plasmid. Objective: This study aimed to produce his- and myc- tagged MFE-23 scFv antibodies by using E. coli culture and to detect their solubility by using ELISA assay. Methods: Transformed E. coli containing sequences of MFE-23 coding were inoculated and evaluated for their optical density. An ELISA plate was coated by CEA or PBS and secondary antibodies were anti-his, anti-myc and anti-MFE. Horseradish peroxidase-OPD substrate was added to produce chromatic reaction for qualitative detection. Results: The results showed that each characterized tube was positive for myc-tagged MFE, his- and myc- tagged MFE, and his-tagged MFE for tube 1, 2, and 3 respectively.Conclusion: This study indicated that transformed E. coli culture is a suitable host for MFE-23 svFV production, and qualitative ELISA assay is a simple useful method for antibody detection and characterization of single chain antibodies.
DISTRIBUSI DAN DETERMINAN HIPERTENSI DI KECAMATAN SAMBIREJO, KABUPATEN SRAGEN Pariawan Lutfi Ghazali
JKKI : Jurnal Kedokteran dan Kesehatan Indonesia JKKI, Vol 10, No 1, (2019)
Publisher : Faculty of Medicine, Universitas Islam Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20885/JKKI.Vol10.Iss1.art6

Abstract

Background: One of the most prevalent degenerative diseases is hypertension. The statistic data of Sambirejo sub-district of Sragen Regency shows that hypertension is the disease with the second largest number of patients. Research on the distribution and determinant of hypertension needs to be done to support intervention efforts.Objective: This study aims to determine the distribution and determinants of hypertension in Sambirejo sub-district, Sragen Regency.Methods: This observational study used a cross-sectional design, with descriptive analytic types. The sample was taken using a stratified random sampling technique. Blood pressure measurements using sphygmomanometer, and other data obtained from questionnaires and direct measurements. We tested the relationship between variables with Kruskal-Wallis bivariate analysis. The multivariate analysis used is Ordinal Regression. Data were statistically significant if p <0.05 (CI: 95%).Results: The study sample was 454 people. Respondents who experienced hypertension were very high at 57.2%. Hypertension is more prevalent in men, and ages 40 and above. Determinant factors of hypertension are age, gender, genetics, exercise habits, smoking habits, fruit and vegetable consumption, and body mass index. Multivariate analysis with ordinal regression test showed the factors to influence the hypertension were genetic factors (OR=4,777; 95% CI 3,232-7,059; p=0,000), smoking (OR=1,004, 95%CI: 1,003 – 1,004, p=0,0001), and body mass index. (OR=1,054; 95%CI:1,002 – 1,109, p=0,042).Conclusion: Hypertension distribution is more dominant in the male group, and =40 years of age. Determinants of hypertension are age, sex, genetics, exercise habits, smoking habits, fruit and vegetable consumption, and body mass index. Genetic factors are the dominant determinant for hypertention.
Penghambatan Ekstrak Metabolit Sekunder Streptomyces sp. dari Sedimen Tanah Kawasan Hutan Mangrove Hamadi terhadap Plasmodium falciparum Secara in vitro Semuel Sandy; Iman Harisma Saleh Sasto
JKKI : Jurnal Kedokteran dan Kesehatan Indonesia JKKI, Vol 11, No 1, (2020)
Publisher : Faculty of Medicine, Universitas Islam Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20885/JKKI.Vol11.Iss1.art6

Abstract

Background: Reports about the resistance of antimalarial drugs demand exploration and development of new drugs. Indonesia's vast maritime area and abundance of mangrove forests have the potential for the development and discovery of active anti-cancer substances, antibiotics and antiplasmodial. Studies on the potential of new drugs from this sector are still limited. Objective: The purpose of this study was to see the potential of Actinomycetes genus Streptomyces sp. as an antiplasmodial in the Hamadi Coast of Papua.Methods: Retrieval of mangrove soil sediments to isolate Streptomyces was located in the Hamadi-Jayapura mangrove forest. Selective isolation was conducted by SCA (Starch Casein Agar) selective media. Identification of Streptomyces sp was examined microscopically through observation of colony morphology and gram staining. The results of isolation of Streptomyces were then fermented on FM3 media until finally secondary metabolite extract of Streptomyces was obtained. The secondary metabolite extract of Streptomyces was tested for its inhibition on the development of Plasmodium falciparum.Result: Analysis of inhibition of the secondary metabolite extract of Streptomyces sp on the development of Plasmodium falciparum showed good results because the extract of Streptomyces sp with a concentration of 100 µg/mL could suppress average growth values of Plasmodium falciparum to only 0.66% with an average inhibitory value of 95.20%. This value followed levels of extract concentration by the lowest concentration of 0.01 µg/mL, the average value of Plasmodium falciparum growth which increased to 10.89% with an average inhibition value of 20.83%. IC50 analysis of Plasmodium falciparum in culture for 48 hours was 0.12 µg/mL, and this value was very good because a test of substance has very good inhibitory activity when the IC50 value is = 10 µg/mL.Conclusion: Streptomyces’ secondary metabolite extract from mangrove sediments showed a very good ability to inhibit the growth of Plasmodium falciparum so that it could have potential as a source of antiplasmodial.

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