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Livestock and Animal Research
Published by Universitas Sebelas Maret
ISSN : 27215326     EISSN : 27217086     DOI : https://doi.org/10.20961/lar.
Core Subject : Health, Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Veterinary
Livestock and Animal Research (Livest. Anim. Res.) publishes original research article in the field of livestock and other animal research. This journal previously titled as Sains Peternakan: Jurnal Penelitian Ilmu Peternakan and changed into Livestock and Animal Research in order to broaden the distribution and increase the visibility of the journal. This journal is published three times a year: March, July, and November by Sebelas Maret University associated with Ikatan Sarjana Peternakan Indonesia (ISPI). The first edition with new journal-title is published in July 2020 (Vol. 18 No. 2) while previous editions remain the same as the previous journal that can be accessed through website: https://jurnal.uns.ac.id/Sains-Peternakan. Livestock and Animal Research has been accredited and categorized as Sinta 2 by Directorate General of Strengthening Research and Development – Ministry of Research, Technology and Higher Education, Republic of Indonesia in 2019 (No. 10/E/KPT/2019). In addition, this journal has been indexed by Crossref, Google Scholar, Mendeley, Garuda (Garda Rujukan Digital), i-Focus, i-Journals, and Udledge.s The interest of Livestock and Animal Research covering: 1) Animal Production System; 2) Animal Breeding and Genetics; 3) Animal Physiology and Reproduction; 4) Animal Nutrition and Feeding Technology; 5) Technology of Animal Product; 6) Animal Biotechnology; 7) Animal Behaviour and Welfare; 9) Socio-Economics in the field of Animal Research, and 10) Animal health. The objects of the research can be livestock, laboratory, exotic, and/or wild animal. This journal uses blind review where all the review process using anonymous system so that both reviewer and author identity are not revealed during the process.
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Hewan dan Zoologi
Articles 160 Documents
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Selection of Lactiplantibacillus plantarum strains as inoculant of rice straw fermentation and its fermentation characteristics Mernawati Mernawati; Anja Meryandini; Yantyati Widyastuti
Livestock and Animal Research Vol 21, No 3 (2023): Livestock and Animal Research
Publisher : Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/lar.v21i3.65024

Abstract

Objective: This study aimed to select Lactiplantibacillus plantarum strains suitable for inoculant of rice straw fermentation and to evaluate its fermentation characteristics.Methods: The experiment was conducted at the Feed Bioprocess Laboratory, Research Center for Applied Zoology, National Research and Innovation Agency (BRIN). This experiment was designed using a 3×4 factorial arrangement in a completely randomized design (CRD). The first factor was inoculant (1%), and the second was incubation time (7, 14, 28, and 58 days). The first stage was selection of 9 strains of L.  plantarum, based on the ability to lowering the pH and produce lactic acid. The second stage was observing the growth curve of the selected strains of L. plantarum as inoculant for rice straw fermentation. The third stage was monitoring of the rice straw fermentation characteristics, including pH values, concentration of lactic acid, water-soluble carbohydrates (WSC) and NH3-N, also dry matter, ash and crude protein. Data were analyzed based on analysis of variance, and if there was a significant effect, the data were further analyzed with Duncan’s Multiple Range Test.Results: The selected strains L. plantarum Ca098 and L. plantarum 1A-2 showed significant interaction result (p<0.05) compared with treating inoculant and ensiling days. The fermentation time influenced (p<0.05) the quality of rice straw fermentation including pH, concentration of lactic acid, WSC, NH3-N, and crude protein.Conclusions: L. plantarum Ca098 showed the best rice straw fermentation characteristics, with fleigh value of 163.86±5.97 at 58 days of fermentation. L. plantarum Ca098 showed the highest lactic acid, the lowest pH and improve crude protein content.
Retrospective analysis of Marek's disease virus outbreak in 2013 on chicken farms in North Sumatra, Indonesia Risza Hartawan; Diana Nurjanah; Teguh Suyatno; Any Purwany; Atik Ratnawati; Risa Indriani; Indrawati Sendow; Ni Luh Putu Indi Dharmayanti
Livestock and Animal Research Vol 21, No 3 (2023): Livestock and Animal Research
Publisher : Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/lar.v21i3.78210

Abstract

Objective: Marek's disease is lymphoma in chicken caused by Marek's disease virus serotype 1 (MDV-1), characterized by paralysis and tumour formation. The disease has caused enormous economic loss worldwide, including in Indonesia. Unfortunately, molecular data related to MDV-1 from Indonesia is limited. Therefore, this study aimed to identify genetic characteristics of MDV-1 causing outbreaks in commercial farms in North Sumatra, Indonesia, 2013.Methods: The amplification of Meq, pp38, gC, and gE genes of the strain MDN2013 was carried out using the PCR protocol. Fragment gene sequencing was accomplished using the Sanger method. Subsequently, the bioinformatic analyses of BLASTN and phylogenetic tree were applied to analyze the molecular characteristics of these gene sequences.Results: The Meq, pp38, gC, and gE genes of the strain MDN2013 were successfully sequenced and submitted to the NCBI GenBank database. The Meq genes share a high identity with Asian viruses, mainly those originating from Indonesia, India, and China. The pp38, gC, and gE genes share similarities with other viruses in more diverse regions in Asia, Africa, Europe, and America. The phylogenetic tree analysis of the Meq gene demonstrated specific patterns for pathotype biodiversity where the strain MDN2013 has been categorized as virulent up to very virulent virus.Conclusion: In conclusion, the molecular approach is an effective tool for investigating the MDV-1 biodiversity in the field, especially the Meq gene sequence analysis.
Physical structure of leather tanned with aluminium as an alternative tanning agent Emiliana Anggriyani; Nais Pinta Adetya; Laili Rachmawati; Nurwantoro Nurwantoro
Livestock and Animal Research Vol 21, No 3 (2023): Livestock and Animal Research
Publisher : Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/lar.v21i3.66093

Abstract

Objective: This study aims to examine the physical structure of goat skins tanned with aluminium as an alternative tanning agent.Methods: Twelve pieces of goat skin from the pikle breed were used in the study. Chromosal B, an aluminium tanning agent, Novaltan Al, salt (NaCl), Derminol OCS, MgO, Sodium bicarbonate, a BCG indicator, Permit MLN, and an anti-fungal are among the chemicals utilized. The approach involves tanning using chrome tanning as a control and aluminum tanning with amounts of 2%, 4%, and 6% Al2O3. Cross-sectional tests were used to assess the wet white leather's results, and the SEM-EDX method was used to determine the leather's composition.Results: Leather tanned with aluminium tanning agent shows the distribution of aluminium in the skin section, the increasing use of aluminium tanning materials, the higher the aluminium content in tanned leather.Conclusions: The presence of aluminium tanning agent in the skin indicates an interaction between the material and the skin so that it can be used as an alternative tanning agent.
Evaluating sperm quality characteristics obtained through the female teaser method in native chicken breeds Setya Budhi Udrayana; Kasianto Kasianto; Iswati Iswati; Intan Galuh Bintari
Livestock and Animal Research Vol 21, No 3 (2023): Livestock and Animal Research
Publisher : Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/lar.v21i3.66495

Abstract

Objective: The quality and quantity of chicken sperm are critical factors influenced by the semen collection method. Therefore, this study aims to evaluate the sperm quality obtained from three native chicken breeds using the female teaser method.Methods: These three native chicken breeds are Arabic, Birma, and Kampung Unggul Balitbangtan (KUB) chickens. Each breed comprised a group of three roosters, and the semen collection was performed ten times. Macroscopic and microscopic evaluations were conducted to assess the chicken sperm. The collected data were analyzed using Analysis of Variance (ANOVA) followed by Duncan's multiple range test (DMRT).Result: The results of this study showed significant differences (P<0.05) in the sperm volume among the chicken breeds, with Birma (0.51 ml) and KUB chickens (0.60 ml) exhibiting distinct volumes compared to Arabic chicken (0.25 ml). Generally, the chicken sperm exhibited a milky white color and thick consistency. Microscopic evaluation yielded a mass motility rating of 3+. Meanwhile, individual motility and viability did not exhibit significant differences (P>0.05) among the three native chicken breeds. Abnormalities displayed significant variations (P<0.05), with Birma chicken showing the highest abnormality rate at 9.77%± 0.28. Sperm concentration did not exhibit significant differences (P>0.05), with the highest concentration observed in Arabic chicken at 4265.33±59.84 (x106 cells/ml). However, total sperm count and total motile sperm count exhibited significant variations (P<0.05), with the highest values recorded in KUB chicken at 2241.82±264.13 (x106 cells) and 1936.27±169.85 (x106 cells), respectively.Conclusions: In conclusion, the semen collection method using the female teaser yielded native chicken sperm with high quality and quantity and meets the required standards for successful insemination. This method holds potential and is recommended for application in native chicken breeding programs.
The influence of objective and subjective knowledge on attitude and willingness to pay veterinary control number-certified livestock food product Suci Paramitasari Syahlani; Tian Jihadhan Wankar; Agung Triatmojo
Livestock and Animal Research Vol 21, No 3 (2023): Livestock and Animal Research
Publisher : Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/lar.v21i3.73266

Abstract

Objective: This study aims to analyse the effect of objective and subjective knowledge on attitude and willingness to pay for veterinary control number certified-livestock food products.Methods: The study was conducted by survey design. Respondents were determined by a judgmental sampling method with the criteria that respondents were at least 19 years old and contribute as full or part decision makers of food buying for him/herself or their families. Data were collected online by using questionnaires and analyzed using structural equation modelling with partial least squares software.Results: The results showed that objective knowledge (β=0.20, t=1.97) positively affected attitude toward VCN-certified livestock food product, while subjective knowledge did not affect (β= -0.10;  t=1.44). Then, attitude toward VCN-certified livestock food product (β=0.29;  t=3.73) affected willingness to pay for VCN-certified livestock food product. Furthermore, willingness to pay was influenced directly by subjective knowledge (β=0.34; t=6.37) but objective knowledge was not influenced significantly (β=-0.07; t=1.26). The R2 value for attitude toward VCN-certified livestock food product was 0.05 and willingness to pay was 0.18.Conclusions: This study concluded that willingness to pay for VCN-certified livestock food product is directly influenced by subjective knowledge, and indirectly by objective knowledge through attitude toward VCN-certified livestock food product.
Cryopreservation of Simmental cattle semen with egg yolk from different avian species and level glycerol of different in tris diluent Yendraliza Yendraliza; Yudi Gusti Rahman
Livestock and Animal Research Vol 21, No 3 (2023): Livestock and Animal Research
Publisher : Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/lar.v21i3.61255

Abstract

Objective: This research was conducted to determine the effect of egg yolk from different avian species and the level glycerol of different in maintaining sperm quality in Simmental cattle in tris diluent.Methods: Semen comes from Simmental (n=3) kept at the Tuah Sakato Artificial Insemination Center (BIB), Payakumbuh, West Sumatra. The diluent used in this research was tris egg yolk derived from three types of egg yolk (Factor A; the egg yolks used to come from ducks, quails and chickens) and different doses of glycerol (Factor B; 6, 6.5 and 7%). The research method used was a Randomized Block Design (RBD) with 2 factors. The shelter sperm is a group. The variables observed were the percentage of motility, abnormality, percentage of live sperm and intact plasma membrane of Simmental cattle sperm after thawing.Results: The results showed no interaction between glycerol levels with types of egg yolk in tris extender of Simmental sperm quality. The tris diluent with duck egg yolk was better than chicken egg yolk which was equivalent which was able to motility up to 52.67%, percentage of live sperm was 70.78, abnormal sperm was only 14.44% and IPM was up to 42.44%. The dose of glycerol 6 ml is better than 6.5 ml and 7 ml resulting in motility up to 52.44%, live percentage reaching 70.22%, abnormal sperm at 14.33% and IPM up to 43%.Conclusion: The use of duck egg yolk in tris extender and the addition of 6% glycerol resulted in better sperm quality in Simmental cattle with high motility, percentage of live sperm, IPM and lower abnormal sperm.
Genetic characterization and lineage analysis of Pasundan (Rancah) cattle in West Java, Indonesia: Insights from ZFY gene sequencing Johar Arifin; Widya Pintaka Bayu Putra; Syahruddin Said; Thobela Louis Tyasi
Livestock and Animal Research Vol 21, No 3 (2023): Livestock and Animal Research
Publisher : Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/lar.v21i3.71611

Abstract

Objective: This study was carried out to characterize Pasundan (Rancah) cattle from West Java of Indonesia based on a partial Zinc Finger Y-linked (ZFY) gene sequence.Methods: Total of fourteen (14) DNA samples of unrelated Pasundan bulls at the breeding station (BPPIBT-SP Ciamis, West Java) were used for sequencing analysis. The blood samples were collected from jugular vein of each bull for the DNA extraction analysis. Along 979 bp of ZFY gene was amplified using primer pairs of forward: 5’- GGT GAG GGC ACA TGA GTT C -3’and reverse:  5’- CTC TGC AGG TGG TTG TGT AA -3’. Therefore, a forward sequencing method was performed in the present study to obtain the ZFY gene sequences. Along 714 bp of bovine ZFY gene sequences were used for determining the Neighbor-Joining (NJ) tree for Pasundan bulls with 1000 × bootstrap replications using a MEGA computer program. The genetic diversity parameters of haplotype diversity (Hd), nucleotide diversity (pi), Tajima’s D test and Fu’s Fs statistics were calculated using a DNAsp computer program.Results: Total of fourteen (14) haplotypes of ZFY gene and seventy (70) mutation sites were detected in the Pasundan bulls. The haplotype diversity (Hd) and nucleotide diversity (pi) in Pasundan’s ZFY gene diversity were 1.00 (high) and 0.01 (low), respectively. The neutrality test of Tajima’s D and Fu’s Fs statistic values were -1.42 and -6.47, respectively. Bovine ZFY gene can be classified into two clades of ZFY-1 and ZFY-2. Therefore, the Neighbor-joining (NJ) tree (1000×bootstrap) revealed that the Pasundan cattle are classified into clade ZFY-1 together with Bos taurus (AF241273, AF465179). Meanwhile, a clade ZFY-2 consisted of Bos taurus (AF465181, DQ336536), Bos indicus (DQ336537), and Bos javanicus (DQ336578).Conclusion: The ZFY gene of Pasundan bulls were polymorphic with seventy mutation sites. Therefore, Pasundan bulls were characterized into ZFY-1 clade or Taurine lineage based on ZFY gene sequence variaton. The crossbreeding program with Bos taurus straw to increase meat production of cattle may affecting the genetic structure of Pasundan bulls since this program is widely applied in West Java through many years ago.
Front Matter Livestock and Animal Research Vol. 22 No. 1, March 2024 Editor in Chief
Livestock and Animal Research Vol 22, No 1 (2024): Livestock and Animal Research
Publisher : Universitas Sebelas Maret

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Abstract

Experimental animal models for polycystic ovarian syndrome (methods, effects, and implications) Erna Yovi Kurniawati; Noor Pramono; Syarief Thaufik Hidayat; Endang Mahati
Livestock and Animal Research Vol 22, No 1 (2024): Livestock and Animal Research
Publisher : Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/lar.v22i1.79197

Abstract

Many studies have replicated the clinical and genetic features of polycystic ovarian syndrome (PCOS) using a range of experimental animal models to improve treatment outcomes. This article aims to present an overview of the various experimental animal models that have been used in PCOS research. In this study, we conducted a systematic review of relevant research articles on the induced animal model PCOS. We searched research articles in Indonesian and English published over the last five years through three databases: PubMed, ScienceDirect, Google Scholar. We use established inclusion and exclusion criteria to select suitable articles. Out of 19 research articles included in our systematic review, we found the animal model PCOS based Rotterdam criteria, PCOS-IR model, PCOS-Inflammation model, PCOS-Gut microbiota model and PCOS-syndrome metabolic model. Androgen agents such as testosterone propionate, free testosterone, DHEA, and letrozole, as well as sodium valproate, are effective in the induction of PCOS phenotypes based on the Rotterdam criteria (oligo/amenorrhea, hyperandrogenic, and polycystic ovaries).
Farmers’ behavior and the potential results of cattle-oil palm integration in South Sumatra's oil palm replanting area Herwenita Herwenita; Joni Karman; Sidiq Hanapi; Fuadi Irsan; Yeni Eliza Maryana; Agus Suprihatin; Yanter Hutapea; Suparwoto Suparwoto
Livestock and Animal Research Vol 22, No 1 (2024): Livestock and Animal Research
Publisher : Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/lar.v22i1.70731

Abstract

Objective: Replanting of oil palm plantations needs to be done to ensure long-term supply. However, problems arise due to the reduced income of farmers during the non-productive period of oil palm plantations, so there are various farmers’ behaviors in responding to this situation. One effort to increase farmers' income is through cattle-oil palm integration. This paper aims to analyze farmers’ behavior, the factors that influence it, and the potential yield obtained from the integration of cattle into oil palm in replanting fields.Methods: The research was conducted using case studies in Cinta Damai Village, Sungai Lilin District, Musi Banyuasin Regency, South Sumatra, from November 2019 to November 2020. The survey was conducted on 35 farmers to assess behavior and factors that could influence it. Meanwhile, the potential yield of cattle-oil palm integration was measured through a demonstration plot of 1 ha of odot grass planting and processing of cow manure waste. Behavioral data were analyzed using path analysis, while the potential yield was analyzed descriptively.Results: The results showed that farmers have behaviors that support the cattle-oil palm integration business. Factors that directly influence this behavior are land area and socio-economic motivation. Meanwhile, socio-economic motivation through success belief has a significant effect on farmers’ behavior.Conclusions: The farmers' behaviour regarding the implementation of cattle-oil palm integration in oil palm replanting areas had criteria of poor, medium and good, with percentages of 5.71%, 25.71% and 68.51% respectively. The factors that directly influence this behaviour are the area of oil palm replanting area and the socio-economic motivation of the farmers. The potential results obtained from implementing cattle-oil palm integration are 1) forage for livestock (FFL) from odot grass, and 2) manure and liquid organic fertilizer (LOF) from cow dunk.

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