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Journal of Food and Pharmaceutical Science

jfps
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Published by Universitas Gadjah Mada

ISSN : 20897200 EISSN : 23390948 DOI : https://doi.org/10.22146/jfps.581

Core Subject : Health, Science,

Biochemistry, Genetics & Molecular Biology Chemistry Immunology & microbiology Medicine & Pharmacology

FOCUS AND SCOPE Journal of Food and Pharmaceutical Sciences offers scientists, researchers, and other professionals to share knowledge of scientific advancements. The journal will publish original research articles, review articles, short communication, and letter to editor. The area of focus should cover all aspects of food and pharmaceutical sciences. The range of topics covered in the journal include: New Horizons in Food Research Food Chemistry Integrated Food Science Health, Nutrition, and Food Food Engineering, Materials Science, and Nanotechnology Toxicology and Chemical Food Safety Food Microbiology and Safety Drug Discovery Computational Chemistry and Molecular Modeling Pharmaceutical Biotechnology and Protein-Peptide Chemistry Pharmaceutics, Biopharmaceutics, Drug Delivery, and Pharmaceutical Technology Pharmaceutical Nanotechnology Pharmacokinetics, Pharmacodynamics, and Drug Transport Metabolism Analytical and Bioanalytical Chemistry Pharmaceutical Chemistry Natural Medicine and Nutraceutical Chemical Processing of Pharmaceuticals including Crystallization, Lyophilization, and Chemical Stability of Drugs Immunology, Biochemistry, and Cell and Molecular Biology

Arjuna Subject : Pharmacology, Toxicology and Pharmaceutics - Ilmu Farmasi

Articles 224 Documents

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Development and validation of atomic absorption spectrometry for the determination of zink and mercury analyzer for determination of Mercury in cream cosmetics Rohman, Abdul; Wijayanti, Erni
Journal of Food and Pharmaceutical Sciences Vol 3, No 2 (2015): J. Food Pharm. Sci (April-June)
Publisher : Fakultas Farmasi, Universitas Gadjah Mada

An atomic absorption spectrometry for the quantification of zink (Zn) and mercury analyzerÂ for the quantification of mercury (Hg) levels in cream cosmetic were developed and validated. The method was validated for linearity and range, precision, accuracy, limit of detection (LOD) and limit of quantification (LOQ). The standard curves were linear over the concentration of 0.1-1.2 Âµg/mL (Zn) and 0.05-2.0 Âµg/L (Hg) with coefficient of correlation (r) > 0.99. The detection limits obtained were 2.8614 Âµg/g sample (Zn) and 0.4749 ng/g sample. The quantification limitÂ obtained were 9.5281 Âµg/g sample (Zn) and 1.5827 ng/g sample. The relative standar deviation (RSD) values found to be 8.67 % for its intraday precision and 9.89 % for its interday precision (Zn) and 12.69 % for its intraday precision and 7.17 % for its interday precision (Hg). These RSD values are lower than those required by RSD Horwitz unction. The mean recovery percentage was 94.28 % (for Zn) and 78.65% (for Hg). These developed methods were succesfully used for determination of Zn and Hg in cream cosmetics products.Â Key words: mercury, zink, atomic absorption spectroscopy, mercury analyzer, cream cosmetic

Tiron Mitigates Thioacetamide-Induced Acute Liver Injury Shoeib, Amal Mahmoud; Said, Eman; Nader, Manar Ahmed; Salem, Hatem Abd-Elrahman; Ammar, Elsayed Mohamed
Journal of Food and Pharmaceutical Sciences Vol 3, No 3 (2015): J. Food Pharm. Sci (September-December)
Publisher : Fakultas Farmasi, Universitas Gadjah Mada

Acute liver injury is a crippling disorder accompanied by extensive impairment of liverâ€™s synthetic, metabolic and detoxifying functions. Tiron is a synthetic vitamin E analog, proven to be anti-oxidant. This study was undertaken to investigate the protective activity of tiron against thioacetamide (TAA)-induced acute liver injury. Rats were orally treated with tiron (300Â mg/kg) for eight days prior to I.V. TAA either (70 mg/kg) or (280 mg/kg) to induce acute liver injury. Biochemical evaluation of hepatotoxicity indices, oxidative stress, cytotoxicity and inflammatory marker: interleukin-6 (IL-6) was conducted along with histopathological examination. Meanwhile, tiron was found to mitigate the TAA-induced elevation of ALT, AST and ALP. However, serum albumin levels mildly improved. Tiron restored liver GSH contents and maintained liver SOD activity. Moreover, tiron significantly reduced the level of serum IL-6. In context, histopathological examination revealed that tiron slightly reduced the extent of TAA-induced necrosis. Tiron has manifested the observed hepatoprotective effect probably by manipulating inflammatory response of liver to injury via downregulating the expression of inflammatory IL-6 and alleviating oxidative stress.Â Â

ELECTRICAL STUNNING AND PRE-SLAUGHTERING TIME INTERVAL EFFECT IN EXPRESSION OF HEART BROILER CHICKENS PROTEIN Hermanto, Sandra
Journal of Food and Pharmaceutical Sciences Vol 3, No 3 (2015): J. Food Pharm. Sci (September-December)
Publisher : Fakultas Farmasi, Universitas Gadjah Mada

Pre-slaughtering process by transporting broiler chickens from farm to slaughterhouse and treatment of electrical stunning could be one of halal critical point. Stressing on chickens could be trigger a specific protein expression or increase its expression as a result of changes in metabolism that occurs. The purpose of this study was to identify the effect of treatment of electrical stunning and the pre-slaughtering interval time on broiler chicken liver protein expression through the analysis of protein profile by SDS-PAGE. Experiments conducted on 7 broiler chickens aged 35 days were given pre-slaughter treatment are different. Treatments include electrical stunning of 0 V, 25 V and 250 V as well as the pre-slaughtering time interval 0 hours, 2 hours, 2 hours + 12 hours (fasting) and 2 hours + 12 (fasting) with electrical stunning 250 V. Each of samples (chicken liver protein) isolated by homogenized in Trs-HCl buffer and the protein concentration determined by Lowry method. Identification of the protein profile was done by observing the differences in the intensity of the electrophoresis results followed by densitometry analysis. The results showed significant differences in outcomes electrical stunning treatment as indicated by the appearance of protein bands at around 109 kDa and do not appear on other treatments. The difference is predicted from particular protein expressed induced by cell metabolism responsive in chicken liver cell.

Optimization of Self-nanoemulsifying Drug Delivery System for Pterostilbene Puspita, Oktavia Eka; Suwaldi, Suwaldi; Nugroho, Akhmad Kharis
Journal of Food and Pharmaceutical Sciences Vol 4, No 2 (2016): J. Food Pharm. Sci (May-August)
Publisher : Fakultas Farmasi, Universitas Gadjah Mada

Solubility is prerequisite for drug absorption across absorptive cell lining the small intestine. It is a problem for poor water soluble drug because limiting its bioavailability when administered by oral route. Lipid based delivery system such as self-nanoemulsifying delivery system (SNEDDS) can be utilized in improving its solubility so that better bioavailability is achieved. Pterostilbene has extremely low solubility in water then become its limiting factor for the bioavailability. This research developed SNEDDS for oral delivery of pterostilbene. Optimum composition of SNEDDS formulation was judged by its dispersion efficiency and clarity when dispersed in water. The efficiency of this formula in enhancing bioavailability was assessed by in vitro digestion model to predict its bioavailability by determining its bioaccessibility. The result showed that optimum composition of SNEDDS was achieved by soybean oil-CroduretÂ® 50-Span 80-PEG 400 in ratio of 16.37 %, 32.07 %, 11.56 %, and 40 %, respectively. This formula has bioacessibility of 91.48 Â± 2.18 %, and it is much higher compared to pterostilbene that was not formulated into SNEDDS, i.e 4.63 Â± 1.11 %. Determined by dynamic light scattering, this optimum formula has droplet size of 31.8 nm when dispersed in water.

Antioxidant Activity Test Of Total Flavonoids Mistletoe Leaves (Dendrophthoe pentandra (L.) Miq) From False Ashoke Tree Fahmi, Aliyah; NST, Rumondang Bulan; N, Hamonangan
Journal of Food and Pharmaceutical Sciences Vol 4, No 2 (2016): J. Food Pharm. Sci (May-August)
Publisher : Fakultas Farmasi, Universitas Gadjah Mada

Research onÂ the antioxidant activity test of total flavonoidsÂ mistletoe leaves (Dendrophthoe pentandra (L.) Miq) from false ashoke tree (Polyalthia longifolia) had been done. This study aimed to determine antioxidant activity of total flavonoidsÂ mistletoe leaves based on reduction method of free radicals 2.2- diphenyl-1-picrylhydrazyl (DPPH) 200 ppm using a UV Visible Spectrophotometer at maximum wavelength 516 nm was obtained Inhibition Concentration (IC50) amount 6,16 mg / L with the reduction Â percentage of 98.61% at 100 ppm which means it had a very strong antioxidant activity. Keywords: mistletoe leaves, Dendrophthoe pentandra (L.) Miq, antioxidant activity ,false ashoke tree

Determination of Rifampicin, Isoniazid, Pyrazinamide and Ethambutol Hydrochloride in 4FDC Tablet by FTIR Spectrophotometry in Combination with Multivariate Calibration Kurniati, Zakiah; Riyanto, Sugeng; Rohman, Abdul Abdul
Journal of Food and Pharmaceutical Sciences Vol 4, No 2 (2016): J. Food Pharm. Sci (May-August)
Publisher : Fakultas Farmasi, Universitas Gadjah Mada

This study was aimed to develop a fast, inexpensive, simple, and not involving hazardous reagent for the determination of rifampicin (RIF), isoniazid (INH), pyrazinamide (PZA) dan ethambutol hydrochloride (ETH) in 4FDC tablet based on infrared spectrophotometry in combination with chemometrics of partial least square (PLS) calibration. The analytical procedure involves three steps, namely calibration, validation and sample assay. The multivariate calibration model is evaluated by determining the precision and accuracy parameters, as suggested by IUPAC. The results showed that the optimized wavenumber range for the determination of RIF was the combined wavenumbers of 433 â€“ 1552 cm-1 and 1756 â€“ 3412 cm-1, the wavenumber of 433 â€“ 873 cm-1 was selected for quantification of INH, 1714 â€“ 2756 cm-1 for PZA, and 1552 â€“ 2970 cm-1 for ETH. The predicted residual error sum of square (PRESS) values of RIF, INH, PZA and ETH contents were 0.00392%, 0.011724%, 0.007604%, and 0,003368%, respectively. The root mean square error of prediction (RMSEP) values obtained for analysis of RIF, INH, PZA and ETH were 0.01979%, 0.03424%, 0.027575% and 0.018351%, respectively. The values of coefficient of determination (R2) for the relationship between actual values of these drugs and calculated values of these drugs using FTIR spectroscopy were 0.990, 0.977, 0.992 and 0.998 for RIF, INH, PZA and ETH, respectivelty. The developed method was succesfully applied for the determination of 4FDC tablet. The contents of RIF, INH, PZA and ETB in 4FDC tablets were in agreement with those specified in monograph of Indonesian Pharmacopeia.Keywords:Â infrared spectrophotometry, rifampicin, isoniazid, pyrazinamide, ethambutol hydrochloride, partial least square.

OPTIMATION OF COMPOSTION OF SPAN 80-CRODURET 50-PROPYLENE GLYCOL AS EMULSIFIER OF STRAWBERRY JUICE (Fragaria vesca L.) EMULGEL AND TRANSPORT TEST THROUGH SHED SNAKE SKIN MEMBRANE ERMAWATI, DIAN EKA
Journal of Food and Pharmaceutical Sciences Vol 4, No 3 (2016): J. Food Pharm. Sci (September-December)
Publisher : Fakultas Farmasi, Universitas Gadjah Mada

Strawberry (Fragaria vesca L.) contains many nutrients that are beneficial to health because rich of antioxidant such as ascorbic acid, quercetin-3-Î²-Dâ€“glucoside, anthocyanin pelargonidin-3-O-glucoside and cyanidin-3-glucoside. Amount of anthocyanins and flavonoids in strawberry during process will decline respectively 53% for pelargonidin-3-O-glucoside and 40% for flavonoid quercetin. Using water-oil emulsion (w/o) topically less convenient, so it needs to be dispersed in the matrix gel to help sustain the permeation of the active components of strawberry juice into the stratum corneum. Emulsion consisting of water and oil components are not mixed with each other, thus requiring additional emulsifier to create a system that is homogeneous and stable.The proportion of combination of emulsifier as span 80, croduret 50, propylene glycol are optimized, to produce an optimum formula with good physical stability. The optimization method was Simplex Lattice Design by using Design ExpertÂ® software 7.1.5. The results of the response of the assessed physical parameter were viscosity, separation ratio (F), and diameter of emulsion globule. The optimum formula have measured physical stability test, active ingredient of flavonoid and anthocyanins, whichÂ then was conductedÂ transportÂ testÂ through the membrane of shed snake skin by Franzâ€™s model diffusion cells with the flavonoid concentration parameters that were able to pass through the membrane.Emulsion w/o that use combination of 2,00% Span 80; 4,00% croduret 50, and 2,00% of propylene glycol which produce a physically stable emulsion base on Simplex Lattice Design [p-value> 0.05]. Strawberry juice contains 23,3 % of flavonoid, 13,23% of anthocyanin, and IC50 331 Âµg/mL. The optimum formula of emulgel contains 20% of flavonoids and 11.67% of anthocyanin. The cumulative amount of flavonoid which was transported through the shed snake skin membrane was 117,14 Âµg/cm2Â during five hours. The permeability of membrane shed snake skins is 2,84x10-5 Âµg/cm2and flux value is 6,6x10-5 Âµg/secon.Â Keywords : strawberry juice, emulsifier, Simplex Lattice Design, emulgel, flavonoid

Toxicity Test Pediocin N6 Powder Produced from Isolates Pediococcus Pentosaceus Strain N6 on White Mice Ketaren, Nurjama'yah Br.; Marlida, Yetti; Arnim, Arnim; Yuherman, Yuherman; Rusmarilin, Herla
Journal of Food and Pharmaceutical Sciences Vol 4, No 1 (2016): J. Food Pharm. Sci (January-April)
Publisher : Fakultas Farmasi, Universitas Gadjah Mada

Pediocin N6 powder is a bacteriocin the heat resistant derived from isolates Pediococcus pentosaceus strain N6. These were isolated from water source heat Rimbo Panti West Sumatra. Pediocin N6 powder has high antimicrobial activity, so the potential to be used as biopreservatif on meat and food processing industry which involves heating. Toxicity test was conducted to determine the effects of the toxic effect of a single dose of oral Pediocin N6 powder in test animals male white mice to determine the LD50 and see changes in body weight of mice for 15 days of treatment. Changes in body weight of mice was determined by using non factorial experiment in a completely randomized design consists of 4 treatments and 5 replications. The test animals were divided into 4 groups and each treatment consisted of 5 mice. The treatments tested consisted of Pediocin N6 powder 5000 mg/kg body weight, 10,000 mg/kg body weight, 15,000 mg /kg and 20,000 mg /kg body weight of mice. The test results showed that the Pediocin N6 powder up to a dose of 20 000 mg/kg in a single oral dose administration, there are no death of mice to 15 days of treatment. Based on the LD50 value of a single oral dose can not be calculated, based on it can be stated LD50 value pseudo Pediocin N6 powder greater than 20,000 mg /kg in male mice. The average changes in body weight of mice at a dose of Pediocin N6 powder treatment of up to 20,000 mg/kg every 2 days weighing from day 1 to day 15 of 2.1 gr. Based on these tests Pediocin N6 powder safe used as industry biopreservatif on meat and food processing involves heating.Key word: Toxicity Test, Pediocin N6 Powder, White Mice, Biopreservatif on Meat

Optimum Dose and Formulation of Centella asiatica L. Urban Extract Against IgG of Wistar Strain Male Mices which Induced by BCG Vaccine Ermawati, Dian Eka; Sasmito, Ediati; Mufrod, Mufrod; ND., Pramitha Esha; UA., Ni Putu; KD, Anggi; H., Muchammad; S., Aini
Journal of Food and Pharmaceutical Sciences Vol 4, No 3 (2016): J. Food Pharm. Sci (September-December)
Publisher : Fakultas Farmasi, Universitas Gadjah Mada

Objective: Centella asiatica L.Urban contains triterphenoid saphonin, flavonoid, and pectin that believed to have an immunomodulatory effect toward the Immunoglobulin-G in Wistar strain male mices which Induced by BCG vaccine. A study was conducted to find out the optimum dose and formulation of in enhancing on the level of IgG. Itâ€™s therefore expected that Cantella asiatica L.Urban can be used as an immunomodulatory suplement in both animals and human.Methods: Centella asiatica L.Urban were macerated with ethanol 50%. This was an experimental study with the post-test control group design. The samples in this study were 35 Wistar strain male mices which divided into 7 groups: negative control [Aquadest 0,5mL/20g BW]; control immunosupresant [PrednisonÂ® 0,06mg/20g BW]; control immunostimulant [LevamisolÂ® 0,445mg/20g BW]; ethanolic extract of Cantella asiatica L.Urban with a dose of 50mg; 100mg; 150mg; 200mg/Kg B.W were administered orally fo 10 day, after the treatments implemented, BCG [Bacillus Calmette Guerin] vaccine was infected intraperitoneally on day 10th, 14th, 21st, and 28th. The most effective doses of ethanolic extract of Cantella asiatica L.Urban was further studied for its effect on hormonal assay using ELISA reader which compared with the effects of PrednisonÂ® and LevamisolÂ®. Based on simplex lattice design to find the optimum proportion of coefficients a, b, and ab of the equation Y = a (A) + b (B) + ab (A) (B), where A is lactose and B is amylum.Results : Oral administration of ethanolic extract of Cantella asiatica L.Urban at dose of 50mg and 100mg/Kg B.W increased the immunity which showed elevated levels of IgG in the blood serum in Wistar strain male mices that had been induced by BCG vaccine. The optimum formula capsul of Centella asiatica L.Urban extract that use combination of 80% Amylum and 20% Lactose which produce the optimum parameters of disintegration time (â‰¤15minutes) and CV of weight uniformity (â‰¤5%)with the test results had no significant difference to the prediction results of Simplex Lattice Design [p>0.05].Keywords: Â Centella asiatica L.Urban extract, Immunomodulator, simplex lattice design

Optimum Dose and Formulation of Centella asiatica L. Urban Extract Against IgG of Wistar Strain Male Mices which Induced by BCG Vaccine Ermawati, Dian Eka; Sasmito, Ediati; Mufrod, Mufrod; Esha ND., Pramitha; Putu UA., Ni; KD, Anggi; Muchammad H., Muchammad; Aini S., Aini
Journal of Food and Pharmaceutical Sciences Vol 4, No 3 (2016): J. Food Pharm. Sci (September-December)
Publisher : Fakultas Farmasi, Universitas Gadjah Mada

Objective: Centella asiatica L.Urban contains triterphenoid saphonin, flavonoid, and pectin that believed to have an immunomodulatory effect toward the Immunoglobulin-G in Wistar strain male mices which Induced by BCG vaccine. A study was conducted to find out the optimum dose and formulation of in enhancing on the level of IgG. Itâ€™s therefore expected that Cantella asiatica L.Urban can be used as an immunomodulatory suplement in both animals and human.Methods: Centella asiatica L.Urban were macerated with ethanol 50%. This was an experimental study with the post-test control group design. The samples in this study were 35 Wistar strain male mices which divided into 7 groups: negative control [Aquadest 0,5mL/20g BW]; control immunosupresant [PrednisonÂ® 0,06mg/20g BW]; control immunostimulant [LevamisolÂ® 0,445mg/20g BW]; ethanolic extract of Cantella asiatica L.Urban with a dose of 50mg; 100mg; 150mg; 200mg/Kg B.W were administered orally fo 10 day, after the treatments implemented, BCG [Bacillus Calmette Guerin] vaccine was infected intraperitoneally on day 10th, 14th, 21st, and 28th. The most effective doses of ethanolic extract of Cantella asiatica L.Urban was further studied for its effect on hormonal assay using ELISA reader which compared with the effects of PrednisonÂ® and LevamisolÂ®. Based on simplex lattice design to find the optimum proportion of coefficients a, b, and ab of the equation Y = a (A) + b (B) + ab (A) (B), where A is lactose and B is amylum.Results : Oral administration of ethanolic extract of Cantella asiatica L.Urban at dose of 50mg and 100mg/Kg B.W increased the immunity which showed elevated levels of IgG in the blood serum in Wistar strain male mices that had been induced by BCG vaccine. The optimum formula capsul of Centella asiatica L.Urban extract that use combination of 80% Amylum and 20% Lactose which produce the optimum parameters of disintegration time (â‰¤15minutes) and CV of weight uniformity (â‰¤5%)with the test results had no significant difference to the prediction results of Simplex Lattice Design [p>0.05].Â Keywords: Â Centella asiatica L.Urban extract, Immunomodulator, simplex lattice design

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INSTITUTE FOR HALAL INDUSTRY & SYSTEM (IHIS) UNIVERSITAS GADJAH MADA Jl. Kaliurang Km.4 Sekip Utara Yogyakarta 55281

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Home Page

OAI Link

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Contact Name Puma Arfah

Contact Email pm.arfah@ugm.ac.id

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Journal Mail Official jfoodpharmsci.gama@gmail.com

Editorial Address INSTITUTE FOR HALAL INDUSTRY & SYSTEM (IHIS) UNIVERSITAS GADJAH MADA Jl. Kaliurang Km.4 Sekip Utara Yogyakarta 55281

Location Kab. sleman, Daerah istimewa yogyakarta INDONESIA

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Contact Name
Puma Arfah

Contact Email
pm.arfah@ugm.ac.id

Phone
+628129880797

Journal Mail Official
jfoodpharmsci.gama@gmail.com

Editorial Address
INSTITUTE FOR HALAL INDUSTRY & SYSTEM (IHIS) UNIVERSITAS GADJAH MADA Jl. Kaliurang Km.4 Sekip Utara Yogyakarta 55281

Location
Kab. sleman,

Daerah istimewa yogyakarta

INDONESIA