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Jurnal Natural
Published by Universitas Syiah Kuala
ISSN : 14118513     EISSN : 25414062     DOI : https://doi.org/10.24815/jn
Core Subject : Science, Agriculture,
Agriculture, Biological Sciences & Forestry Astronomy Biochemistry, Genetics & Molecular Biology Chemistry Earth & Planetary Sciences Energy Immunology & microbiology Neuroscience Physics
Jurnal Natural (JN) aims to publish original research results and reviews on sciences and mathematics. Jurnal Natural (JN) encompasses a broad range of research topics in chemistry, pharmacy, biology, physics, mathematics, statistics, informatic and electronic.
Arjuna Subject : Matematika - Matematika (Lain-Lain)
Articles 457 Documents
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Isolation and characterization of polyhydroxyalkanoate (PHA) producing bacteria isolate from landfill land of Kampung Jawa Banda Aceh MAZWAN MAZWAN; FEBRIANI FEBRIANI; NAZARUDDIN NAZARUDDIN
Jurnal Natural Volume 23 Number 1, February 2023
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24815/jn.v23i1.29746

Abstract

The production of biodegradable plastic from microorganisms has great potential as a substitute for conventional plastic. This study aims to isolate bacterial strains capable of polyhydroxyalkanoates (PHAs) production from the Kampung Jawa landfill land (KJLL) and characterize biopolymers. The bacterial strains were able to produce PHA using a mineral salt medium (MSM) with glucose as a carbon source. The qualitative screening of PHA-producing bacteria was conducted by Sudan Black and Nile Red. Of the 64 bacteria strains, only 41 were able to accumulate PHA in Sudan Black and Nile Red. The results showed that one bacteria the Coccobacillus strain had the highest color intensity for further characterization of PHA. The characterization of PHA by Differential Scanning Calorimetry (DSC) and Thermogravimetric Analysis (TGA) showed a melting temperature (Tm) of 101.54°C. X-ray diffraction (XRD) analysis revealed a crystalline structure with an index crystallinity (ICr) value of 15.82% for PHA. The results of the analysis proved that PHA was produced by bacteria isolate. This study suggests that this is the first report of the bacteria from the Kampung Jawa landfill producing PHA with good characteristics and potential biotechnology applications. 
Optimizing the gonadal performance of broodstock Helostoma temminckii with addition of enrichment ingredients in feed INDIRA FITRILIYANI; SISWANTO SISWANTO; LUCHAS LUCHAS
Jurnal Natural Volume 22 Number 1, February 2022
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24815/jn.v22i1.23426

Abstract

The problem in the development of Kissing gourami fish culture was quality seeds that cannot be available continuously. Purpose of this research was to examine the need for additional enrichment materials in feed that can optimize the process of gonad maturation of Kissing gourami fish. Treatment A=Commercial feed added with Vegetable Oil, Glutathione, and Egg White; B=Commercial feed added with Vegetable Oil, Vitamin E 300 mg/Kg, Glutathione, and Egg White; C=Commercial feed added with Vegetable Oil, Vitamin E 500 mg/Kg, Glutathione, and Egg White. and D=Commercial feed added with Vegetable Oil, Vitamin E 700 mg/Kg, Glutathione, and Egg White. The results showed that all treatments with the addition of glutathione and vitamin E fortifications with levels of 300 mg-700 mg gave the effect of increasing IGS, IHS, higher fecundity and larger egg diameter than treatment A. The amount of initial IGS increase in treatment A was 33.49%, treatment B was 172.52%, treatment C was 208.60% and treatment D was 691.20%. While the IHS value at the end of the study decreased in treatment A by 35.30% and treatment C which also experienced a decrease in IHS by 26.23%. While in treatments B and D there was a not too large increase, in the range of 42.56% (B) and 5.66% (D).
MODELING AND SIMULATION OF TRAFFIC FLOWS ON INCLINED ROAD DURING EVACUATION PROCESS OF THE VOLCANO DISASTER WITH FINITE DIFFERENCE METHOD Richasanty Septima; Said Munzir; Salmawaty Salmawaty
Jurnal Natural Volume 17, Number 1, March 2017
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24815/jn.v17i1.6513

Abstract

The research in this thesis was done to examine the model of traffic flow of volcanic disaster evacuation path for uphill and downhill roads. The assessment was focused on the area of disaster evacuation path from the Pante Raya Bener Meriah intersection to Takengon. This model is assessed for two different types of time when which a disaster occurs; the disaster occurred at night and the disaster occurred during the day, especially during peak hours (working hours). The model was developed with attention to the exixtence of inflow and outflow along the evacuation route. Furthermore, the model obtained is solved numerically by using finite difference method. The chosen approach of this method is upwind scheme with time and space steps using forward difference and backward difference. The solution of this model in the form of simulated vehicle density along evacuation pathways. The research conducted is in the form of a model of traffic flow on evacuation paths and restricted to the inflow and outflow without alternative path as well as the conditions of the road which are uphill and downhill, showed a high density of vehicles either at night or during the day. Uphill road conditions resulted in decreased vehicle speed and vehicle density will increase, while downhill road conditions resulted in increased vehicle speed and vehicle density will decrease, meaning that the road conditions which are uphill and downhill will greatly affect the process of evacuation. Degree vehicles of evacuation efficiency occuring at night without an alternative pathway produces a high efficiency so that it can be interpreted that the evacuation process in the evening was successful and runs better than the evacuation process during the day, and this is caused by the existence of vehicles on the road  evacuation process started thus affecting the efficiency levels. Keywords : Traffic flows, finite difference method, upwind scheme method, forward difference, backward difference, greenshield method, uphill road, downhill road.
Stevia rebaudiana: Phytochemical, pharmacological activities, and plant tissue culture (a mini-review) FAHRAUK FARAMAYUDA; RENALDI OKTAVIANUS; ELFAHMI ELFAHMI
Jurnal Natural Volume 22 Number 3, October 2022
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24815/jn.v22i3.19477

Abstract

Stevia rebaudiana is very famous as natural sweeteners and potentially for lowering blood sugar. The benefits of Stevia rebaudiana are numerous, and various pharmacological activities have been found, such as efforts to treat diseases such as diabetes, obesity, dental caries, hypoglycemia, and hypertension. The main sweet compounds in Stevia rebaudiana are rebaudioside A and stevioside, especially in the leaves. This review examines aspects of phytochemistry, pharmacological activity, micropropagation, and in-vitro culture modification of Stevia rebaudiana plants. Online and offline literature searches were conducted to compile the articles. PubMed (Medline), Web of Science, is used to retrieve online publications using the following search terms: Stevia rebaudiana, stevia herbs, traditional medicine, pharmacological activities, toxicity, secondary metabolite content, phytochemicals, and plant tissue culture of Stevia rebaudiana . This review is also a compilation of four previous reviews and is accompanied by the latest data from research journals on Stevia rebaudiana.
Identification of amylase activity from vannamei shrimps’ (Litopenaeus vannamei) digestive tract using size exclusion chromatography method HARIYANTI HARIYANTI; HANIFAH RAHMI
Jurnal Natural Volume 21 Number 3, October 2021
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24815/jn.v21i3.18874

Abstract

Vannamei shrimps (Litopenaeus vannamei), also known as white leg shrimps are widely cultivated and consumed by many people. However, most consumers have removed the shrimp’s head which integrates with its digestive tract. The digestive tract of white leg shrimp contains digestive enzymes, including amylase. This study aimed to determine the protein content and amylase activity from Vannamei shrimps’ digestive tract using the size exclusion chromatography method. The protein isolation of size exclusion chromatography was prepared in three steps, namely; centrifugation, precipitation, and dialysis. The protein from the dialysis step was purified by using gel filtration chromatography. Each obtained fraction was determined the protein content and amylase activity by using spectrophotometer UV-Vis method. The results showed that the fraction of 108th had the highest protein content and amylase activity with a value of 0.85 mg/ml and 25.66U/ml respectively. @font-face {font-family:"Cambria Math"; panose-1:2 4 5 3 5 4 6 3 2 4; mso-font-charset:0; mso-generic-font-family:roman; mso-font-pitch:variable; mso-font-signature:-536869121 1107305727 33554432 0 415 0;}p.MsoNormal, li.MsoNormal, div.MsoNormal {mso-style-unhide:no; mso-style-qformat:yes; mso-style-parent:""; margin:0cm; mso-pagination:widow-orphan; font-size:12.0pt; font-family:"Times New Roman",serif; mso-fareast-font-family:"Times New Roman"; mso-ansi-language:EN-US;}.MsoChpDefault {mso-style-type:export-only; mso-default-props:yes; font-size:10.0pt; mso-ansi-font-size:10.0pt; mso-bidi-font-size:10.0pt; mso-ansi-language:IN; mso-fareast-language:IN;}div.WordSection1 {page:WordSection1;}
THE EFFECT OF ARBUSCULAR MICHORRIZA FUNGI AND ORGANIC FERTILIZER ON THE GROWTH OF SUNFLOWER (HELIANTHUS ANNUUS L.) ON CRITICAL LAND OF FORMER IRON ORE MINE IN LHOONG - ACEH BESAR Suwarniati Suwarniati; Ulia Hanum; muslim muslim
Jurnal Natural Volume 18, Number 2, June 2018
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24815/jn.v18i2.11061

Abstract

The study determines the effect of arbuscular mycorrhiza fungi (AMF) and organic fertilizer on sunflower growth (Helianthus annuus L.) in former iron ore mine in Lhoong, Aceh Besar. The Randomized block design is 2×3 factorial with 4 replications was used. The first factor: Without Mycorrhiza and Mixed Mycorrhiza. The second factors were Without Fertilizer, Manure, and Guano. The results showed that at α = 5% variation analysis, AMF had very significant effect on plant height in 10 d After Planting (DAP), leaf number 50 DAP, fresh  weight of top-trimmed, and AMF colonized roots. Furthermore, it showed a significant effect on plant height 40 and 50 DAP, leaf number 20, 40 DAP and dry weight of top-trimmed. Fertilizer were significantly affected on plant height in 10 and 20 DAP, leaf number 50 DAP and fresh/dry weight of the top-trimmed. It showed a significant effect on plant height 30 DAP and leaf number 40 DAP. Mixed Mycorrhiza and Guano showed the best results. The interaction between AMF and fertilizer had a significant effect on plant height at 50 DAP and fresh  weight of the top-trimmed. Mixed Mycorrhiza and Manure showed the best results on 50 DAP with increasing in plant height, while Mixed mycorrhiza and Guano increased fresh weight of the top-trimmed.Keywords: arbuscular mycorrhiza fungi (AMF), Helianthus annuus L., critical land, organic fertilizer, sunflower growth.
Aquifer layer in Muara Batu and Dewantara Sub-district based on resistivity cross-sectional model SYAFRIZAL IDRIS; MULIANI MULIANI; NANDA NOVITA
Jurnal Natural Volume 23 Number 2, June 2023
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24815/jn.v23i2.23921

Abstract

This study aims to determine the depth of the aquifer layer in the Muara Batu and Dewantara sub-district as a source of groundwater that can be used continuously. The identification of this aquifer layer is based on the results of the resistivity values of subsurface rocks measured using the electrical resistivity method of the Schlumberger array. Data acquisition was carried out on 3 lines (MB1, MB3, and MB4) with a length of 330 m each. The variations in the resistivity values of the rocks obtained were modeled in the form of a 2D cross-section using the Res2DInv software, thus providing an overview of the subsurface for groundwater exploitation. The cross-sectional model obtained shows that the shallow aquifer layer is at a depth of 20-52 m (2-12 Ωm) on the MB1 and a depth of 60 m on MB3 and MB4. The low resistivity value 12 Ωm on the MB1 indicates that the subsurface is generally composed of water-saturated rock layers.
Isolation and characterization of cellulose from rice husk waste and sawdust with chemical method RIZKI ANDALIA; RAHMI RAHMI; JULINAWATI JULINAWATI; HIRA HELWATI
Jurnal Natural Volume 20 Number 1, February 2020
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24815/jn.v20i1.12016

Abstract

Cellulose had been isolated from rice husk waste and sawdust with chemical method through hydrolysis with HCl 2.5 N and bleaching process with  H2O23%.The yields were 13,45% and 22,75% (w/w) of cellulose from rice husk waste and sawdust, respectively. The chemical treatment caused partial removal of lignin and hemicellulose from raw material showed by FTIR spectrum.  Structure and morphology of cellulose from rice husk waste and sawdust were investigated using X-Ray Diffraction (XRD) and Scanning Electron Microscopy (SEM). The XRD patterns showed cellulose structure converted from amorphous to crystalline form after bleaching process while SEM images showed that the isolated cellulose from rice husk waste had rougher surface than cellulose from sawdust. The SEM micrograph also showed that the isolated cellulose from rice husk was in the form of aggregates.
Utilization of shallot extract and administration of indole acetic acid to Chrysanthemum sp in vitro culture DAFNI MAWAR TARIGAN; WAN ARFIANI BARUS; FEMIL YANDA HAKIM NASUTION; ANGGRIA LESTAMI
Jurnal Natural Volume 22 Number 2, June 2022
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24815/jn.v22i2.25778

Abstract

Chrysanthemum sp is a plant that has the potential to be developed in Indonesia, apart from being an ornamental flower, it can also be used as a herbal medicine. One of the efforts to develop chrysanthemum can be done in vitro by using organic growth regulators such as shallot extract and indole acetic acid (IAA). The research aims to identify the effect of shallot extract and IAA on the growth of Chrysanthemum sp. The research was conducted at UPT Central Horticulture Seed Center, Medan Johor, in December 2019 - January 2020. The research used a factorial Completely Randomized Design (CRD) with 2 factors, namely the first factor was shallot extract which consisted of 4 levels, namely 0, 35, 70, and 105 g/L water. The second factor was IAA which consisted of 4 levels, namely 0.6, 0.9, 1.2, and 1.5 mg/L water. The results showed that the administration of shallot extract had no significant effect on the growth of chrysanthemum, but the administration of IAA had a significant effect on the number of leaves and plant height with the best concentration at 0.6 mg/L water. The interaction of the two treatments also had no significant effect on the growth of chrysanthemum.
ISOLATION AND ACTIVITY ANTIOXIDANT TEST OF COCOA POD HUSK ETHYL ASETAT EXTRACTS (Theobroma cacao L) Binawati Ginting; Ilham Maulana; Nurdin Saidi; Syarifah Yanti Astryna
Jurnal Natural Volume 19 Number 2, June 2019
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24815/jn.v19i2.12568

Abstract

Isolation and testing of antioxidant activity with1,1-difenil-2-pikril hidrazil (DPPH) from ethyl acetate extract ofcocoa pod husk(Theobroma cacao L) has been carried out.Theobroma cacaoextract (TCE) showed highly strong antioxidant activity with IC50 = 8,75 ppm and vitamine C = 6,07 ppm as positive control. Isolation of the active components of TCE by column chromatography using silica gel absorbent 60 mesh ASTM (Merck 774) and the eluent n-hexane: ethyl acetate (9:1), obtained 7 fraction combined (TCE 1 to TCE 7). There are 6 combined fractions having the potential as antioxidants, namely TCE 2 to TCE 7 with a range of IC50 (6,46 ppm – 91,8 ppm). TCE 2 fraction has a very strong antioxidant activity with IC50 = 6,46 ppm. Separation of TCE 2 fraction on silica column chromatography obtained 4 combined fractions (TCE 2.1 to TCE 2.4). The test results of antioxidant activity showed that TCE 2.4 had very strong activity with IC50 = 42,7 ppm.For the TCE 2.2 fraction, preparative TLC was carried out using eluent n-hexane: ethyl acetate (9.5: 0,5) obtained by TCE 2.2.4 isolate with a melting point of 114-120 °C and was a steroid class.

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