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Jurnal Natural
Published by Universitas Syiah Kuala
ISSN : 14118513     EISSN : 25414062     DOI : https://doi.org/10.24815/jn
Core Subject : Science, Agriculture,
Agriculture, Biological Sciences & Forestry Astronomy Biochemistry, Genetics & Molecular Biology Chemistry Earth & Planetary Sciences Energy Immunology & microbiology Neuroscience Physics
Jurnal Natural (JN) aims to publish original research results and reviews on sciences and mathematics. Jurnal Natural (JN) encompasses a broad range of research topics in chemistry, pharmacy, biology, physics, mathematics, statistics, informatic and electronic.
Arjuna Subject : Matematika - Matematika (Lain-Lain)
Articles 457 Documents
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Comparison of interpolation methods based on Geographic Information System (GIS) in the spatial distribution of seawater intrusion MAULINA TANJUNG; SAUMI SYAHREZA; MUHAMMAD RUSDI
Jurnal Natural Volume 20 Number 2, June 2020
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24815/jn.v20i2.16440

Abstract

The study of monitoring seawater intrusion and groundwater quality in a coastal area needs to be done regularly to prevent the clean water crisis problems in the future. Accurate and reliable interpolation of seawater intrusion over a region is the requirement of an efficient monitoring. In this study, different interpolation methods were investigated and compared to determine the best interpolation method for predicting the spatial distribution of seawater intrusion in the coastal area of Banda Aceh. Groundwater electrical conductivity (EC) was analyzed to identify the contamination of seawater intrusion into the coastal aquifers. Four interpolation methods such as Empirical Bayesian Kriging (EBK), Global Polynomial Interpolation (GPI), Inverse Distance Weighting (IDW), and Local Polynomial Interpolation (LPI), were used to create the spatial distribution of the groundwater electrical conductivity. The accuracy of interpolation methods was evaluated by using a cross-validation technique through the coefficient of determination (R2) and the Root Mean Square Error (RMSE). The results showed that IDW performed the most accurate prediction values and the best surface which were indicated by the least RMSE and the highest R2 value. It can be concluded that IDW interpolation method is the best method for interpolating the groundwater electrical conductivity associated with seawater intrusion in the coastal area of Banda Aceh.
QUALITY CONTROL X RAYS MAMMOGRAPHY IN THE EFFORT MITIGATION INCREASING NUMBER OF CANCER PATIENTS Rini Safitri; Evi Yufita
Jurnal Natural Volume 17, Number 1, March 2017
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24815/jn.v17i1.6970

Abstract

Abstract. Early detection of breast cancer is the first step in prevention that can be done by women, therefore when one is diagnosed with breast cancer, the appropriate treatment can be performed quickly and accurately. Early diagnosis of breast cancer can be a way of mitigation in preventing breast cancer through the use of mammography. Bureau of Radiology as said by The Joint Commission on Accreditation of Hospitals (JHCA) stated that one of the responsibilities of the radiology unit is to control the quality of service which aims to minimize the radiographic image repetition factor; as well as maximizes the quality of radiographic image. Quality control tests are an effort that is needed on the mammography X-ray diagnostics tools. This is done to maintain the quality of expected output. The parameters that are included within the radiation output are the magnitude of current and the voltage of tube that are produced; therefore they remained constant and correspond to the recommended standard. Bureau of Radiological Health, as said by JHCA mentioned that to control the quality of image which will minimize the radiographic image repetition and maximizes the quality of radiographic image. Therefore the radiation output will not be dangerous later. The early stage of the quality control test on the machine was conducted by setting all the filtrations which were placed to capture the x-ray on the x-ray plane tube with minimum value. Then, ionization chamber is placed on the test subject points; right after that the distance between it to the radiation source is noted. The x-ray film is place on a film on the compression table of the patient and the distance between film and the focus point is noted. This is then exposed using a target filter Mo/Mo by setting the current as well as variation the voltage and time. The standard voltage measurements are 20-33kVp. This data is from the observations of time exposure; the output value is then noted. The above procedure is conducted from the minimum voltage to the maximum voltage. The output ray is measured for each voltage. The same procedure is conducted to the target filter Mo/RH. The results obtained are that the greater the input voltage and current will subsequently produce greater doses, therefore the exposure has exceeded the standard limit 0.1 mHy with longer exposure time. The HVL density thickness on the mammography X-ray machine determined the quality of the beam and the doses of x-ray exposure on the mammography machine. The output stability of x-ray beam exposure in the mammography machine mode Mo/Mo still fulfill the standard which is the value of 69% Keywords: Quality Control, Sinar-X, mammography, Mp/Mo, Mo/RH
Application of Poisson and negative binomials models to estimate the frequency of insurance claims RINI OKTAVIA; RAHMA ZUHRA; HAFNANI HAFNANI; NURMAULIDAR NURMAULIDAR; INTAN SYAHRINI
Jurnal Natural Volume 23 Number 1, February 2023
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24815/jn.v23i1.26623

Abstract

Generalized Linear Models (GLMs) are a modeling approach that allows the modeling of nonlinear behaviors and non-Gaussian distributions of residues. This approach is very useful for general insurance analysis, where the frequency of claims and the amount of claims distributions are usually non-Gaussian. In this article, the application of Poisson and Negative Binomial models to estimate the frequency of claims of auto insurance is discussed. The accuracy of the models was compared to choose the best model for determining pure insurance premiums using R software. The data used are a secondary dataset which is the motor vehicle insurance dataset from Sweden named dataOhlsson and the motor vehicle dataset from Australia named ausprivauto0405. The results of the exploration of the GLMs model are that Poisson's GLM and Negative Binomial models both are suitable models for estimating the number of claims for the dataOhlsson dataset. Both models have relatively similar parameter estimates, as well as the AIC and BIC values for the dataOhlsson dataset, however, both models are not suitable for estimating the number of claims for the ausprivauto0405 dataset. More investigation using different models is needed to ensure which model is more appropriate for estimating the frequency of insurance claims.
Preferences of fruit-feeding butterfly on bait trap in Soraya Research Station, Leuser Ecosystem, Aceh, Indonesia ITSNATANI SALMA; ZURIANA SIREGAR; ALIA RIZKI; SUWARNO SUWARNO
Jurnal Natural Volume 21 Number 3, October 2021
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24815/jn.v21i3.22844

Abstract

Butterflies are insects that live cosmopolitan. Some butterflies in tropical forests look for food sources from sucking the juice of ripe fruits that have fallen on the forest floor. Fruit-feeding butterflies can adapt in finding food sources, selecting and sucking food effectively. Fruit contains varying concentrations of sugar and nitrogen. The existence of fruit-feeding butterflies in tropical rain forests is influenced by the availability and quality of food sources as well as other supporting factors such as temperature, humidity, and light intensity. The purpose of this study was to identify fruit-feeding butterflies and their preferences for banana and pineapple baits in the Soraya Research Station Area, Leuser Ecosystem, Aceh, Indonesia from September to November 2020. Six forest trail locations were selected as trap locations. A total of 360 cylindrical gauze baited traps (80 cm high and 35 cm diameter) were installed on six trails/line transects. Baited traps were set at three different heights, that is at the understorey level (± 0-2 m), midstorey level (± 5-6 m), and overstorey level (± 10-11 m) with a distance of ± 5-10 m. The distance between location points is ± 250 m. The fruit baits used in this trap were ripe bananas and pineapples. The results obtained were 37 species with 176 individuals trapped on banana bait and 50 species with 183 individuals on pineapple bait. However, the results of the t-test using the Man-Whitney test showed no significant difference between the banana and pineapple bait used.
ANALYSIS OF SILDENAFIL AND ITS DERIVATIVES IN JAMU (HERBAL MEDICINES) USING LC/MS/MS SPECTROSCOPY Rina Kurniaty; Khairan Khairan; Lelifajri Lelifajri
Jurnal Natural Volume 18, Number 3, October 2018
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24815/jn.v18i3.11153

Abstract

Jamu (herbal medicine) is a traditional Indonesian medicine used to nourish, treat, and restore of health, as well as for beauty and fitness. A healthy Jamu is safe when consumed and free from chemicals (in Indonesia it’s called BKO). BKO is a medicinal chemical compound which is intentionally added in jamu (herbal medicine), which aims to increase the accelerate effects of Jamu or herbal medicine from the usual. The aims of this study are to extract, isolate, identify and elucidate the structure of the BKO of sildenafil and it’s derivatives in two herbal preparations circulating in the city of Banda Aceh. Extraction, isolation and identification of sildenafil BKO and its derivatives in jamu were done by using column chromatography (CC) and thin layer chromatography (TLC) meanwhile, the structure elucidation of sildenafil and it’s derivatives were done by Fourier-Transform Infra-Red (FT-IR) and liquid chromatography/mass spectroscopy (LC/MS/MS). The results of extraction, isolation and   identification with a solvent  ethyl acetate: methanol: ammonium hydroxide (25%) (100: 15: 1.5 v/v showed that two jamu circulating in Banda Aceh positively contained BKO sildenafil derivatives, namely thiosildenafil. The FT-IR analysis showed a strong absorption of thiocarbonyl (C=S) group at 1288-1242 cm-1, which is a typical uptake of the thiosildenafil compound. The Spectroscopy LC/MS/MS analysis using electron spray ionization (ESI-MS/MS) showed that the isolate have a similar spectra with thiosildenafil compound [TSLD+H]+ at 491 m/z and [TSLD+Na]+ at 513 m/z and other derivate of thiosildenafil, namely propoxyphenylhydroxylethylthiosildenafil [PHTSLD+H]+ at 533 m/z.
Resistance status and physiological responses of Dactyloctenium aegyptium to diuron herbicide in pineapple plantation RESTI PUSPA KARTIKA SARI; NANIK SRIYANI; YUSNITA YUSNITA; HIDAYAT PUJISISWANTO
Jurnal Natural Volume 22 Number 3, October 2022
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24815/jn.v22i3.25286

Abstract

Diuron herbicide has been used in the pineapple plantation in Lampung, Indonesia, for more than 35 years. It has been realized that the use of herbicides with the same mode of action intensively can speed up the evolution of resistant weeds over a long period of time. This study aimed to determine whether Dactyloctenium aegyptium from pineapple plantation has evolved resistance to diuron and to examine whether the resistance correlates with the weed physiological activities. The study was conducted at the University of Lampung, from September 2018 to March 2019. The study consisted of two stages, i.e. Stage 1: Weed resistance test and Stage 2: Physiological activity test on resistant weed. The study used a split-plot design. The main plot was the origins of weeds (exposed and unexposed to diuron) and thhe supplots was the diuron dose. The result showed that D. aegyptium exposed has high-level resistance to diuron. The physiological activities of D. aegyptium which has a high level of diuron resistance exhibited higher carbon assimilation, stomatal conductance, and transpiration rates than the sensitive D. aegyptium.
Reduction mechanisms of Ag(I) and Au(III) in the synthesis of silver and gold nanoparticles using leaf extract of Terminalia catappa MUHAMMAD ZAKIR; MAMING MAMING; MISKA SANDA LEMBANG; ESTY YURNITA LEMBANG
Jurnal Natural Volume 21 Number 2, June 2021
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24815/jn.v21i2.20677

Abstract

Synthesis of silver nanoparticles (AgNP) and gold nanoparticles (AuNP) was carried out by the reduction method with leaf extract of Ketapang (Terminalia catappa). The biomolecules present in the extract generated the reduction of Ag+ and Au3+ ions from AgNO3 and HAuCl4, respectively. The growth of nanoparticles was monitored by UV-Vis spectrophotometer. The maximum absorption of biosynthesis of AgNP and AuNP were observed in the respective range of 421-431nm and 530-535nm. Those peaks correspond to surface plasmon absorbance of AgNP and AuNP, respectively. Analysis on the functional groups change of the extract by Fourier Transform Infra Red (FTIR) Spectroscopy showed the formation of carbonyl- from hydroxyl-groups which suggested the oxidation and reduction processes involved in the formation of both nanoparticles. The average size distributions determined by PSA (Particle Size Analyzer) are 55-71nm and 18-44nm for AgNP and AuNP, respectively. Morphology of the silver nanoparticles was observed by Scanning Electron Microscope (SEM) and the structure of the compounds was characterized using X-ray Diffraction (XRD). The shape of AgNP varied from triangular, cubic and hexagonal polyshaped, while AuNP were spherical. XRD studies showed that the nanoparticles obtained were crystalline gold and silver.
A PRELIMINARY STUDY OF INVESTIGATING OF COMPOUND GROUP CONTAINED IN ETHANOLIC EXTRACT OF MAHAGONY (Swietenia mahagoni L. Jacq.) SEEDS RELATED TO Α-GLUCOSIDASE INHIBITION Anjar Purba Asmara
Jurnal Natural Volume 18, Number 2, June 2018
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24815/jn.v18i2.9236

Abstract

A preliminary study to determine the group of compound contained in the ethanolic extract of mahagony (Swietenia mahagoni L. Jacq.) seeds and its inhibitory activity to a-glucosidase enzyme has been done. The information from this study will be used in the further investigation about the specific constituents related to the bioactivity. The seed was grounded and then extracted with ethanol by maceration technique. The crude extract was separated with liquid-liquid extraction by using n-hexane, ethyl acetate, and methanol as the solvents. The best eluent for isolation, chloroform:ethanol (1:1), was determined by thin layer chromatography while alumina as stationary phase. The isolation step with column chromatography gave two types of isolates, yellow and colorless isolate. In order to get information about the compound, the crude extract was subjected to phytochemical assignment and the isolate with the better activity was analyzed by infrared spectroscopy. The inhibitory activity for the yellow isolate with IC50 as 19.345 ppm was better than the colorless isolate. Therefore, the IR spectroscopy assay was subjected to the yellow isolate. Based on the comparison IR spectra with literatures, it has suggested that the yellow isolate contains tetranortriterpenoid or limonoid group.Keywords: Swietenia mahagoni L. Jacq., diabetes type 2, a-glucosidase, tetranortriterpenoidREFERENCE World Health Organization. Global report on diabetes. http://www.who.int. Accessed on 18 July 2017.Kementerian Kesehatan Republik Indonesia. 2014. Situasi dan analisis diabetes. Jakarta: Pusat Data dan Informasi Kemenkes RI.Dutta, M., Raychaudhuri, U., Chakroborty, R., and Maji, D. 2011. Role of diet and plants on diabetic patients - a critical appraisal. Science and Culture. 77 (3–4).Eid, A.M.M., Elmarzugi, N.A., and El-Enshasy, H.A. 2013. A Review on the phytopharmacological effect of Swietenia macrophylla, Int J Pharm Pharm Sci. 5 (3): 47-53.Bera, T.K., Chatterjee, K., Jana, K., Ali, K.M., De, D., Maiti, M., and Ghosh, D. 2012. Antihyperglycemic and antioxidative effect of hydro-methanolic (2:3) extract of the seed of Swietenia mahagoni (L.) Jacq. in streptozotocin-induced diabetic male albino rat: an approach through pancreas. Genomic Medicine, Biomarkers, and Health Sciences. 4: 107-117.Li, D.D, Chen, J.H, Chen, Q, Li, G.W, Chen, J, and Yue, J.M. 2005. Swietenia mahagony extract shows agonistic activity to PPAR-γ and gives ameliorative effects on diabetic db/db mice. Acta Pharmacol Sinica. 26 (2): 220-222.Sathish, R., Natarajan, K., and Selvakumar, S. 2010. Antidiabetic activity of Swietenia mahagoni seed powder in alloxan induced diabetic mice. Research J. Pharmacology and Pharmacodynamics. 2(4): 296-299.Wresdiyati, T., Sa’diah, S., and Winarto, A., Febriyani, V. 2015. Alpha-glucosidase inhibition and hypoglycemic activities of Sweitenia mahagoni seed extract. HAYATI Journal of Biosciences. 22 (2): 73-78.Sukardiman, Riza, N.F., Rakhmawati,  Studiawan, H., Mulja, H.S., and Rahman, A. 2013. Hypoglycemic activity of 96% ethanolic extract of Andrographis paniculata Nees. and Swietenia mahagoni Jacq. combination, E-Journal Planta Husada,. 1.Masitha, M. 2011. Skrining aktivitas penghambatan enzim α-glukosidase dan penapisan fitokimia dari beberapa tanaman obat yang digunakan sebagai antidiabetes di indonesia. Skripsi. FMIPA UI.Aliyan, A.H. 2012. Uji penghambatan aktivitas alfa-glukosidase dan identifikasi golongan senyawa kimia dari fraksi aktif ekstrak biji mahoni (Swietenia macrophylla King). Skripsi. FMIPA UI.Ibrahim, M.A., Koorbanally, N.A., and Islam, M.S. 2014. Antioxidative activity and inhibition of key enzymes linked to type-2 diabetes (a-glucosidase and a-amylase) by Khaya senegalensis. Acta Pharm. 64: 311–324.Harborne, J. B. 1984. Phytochemical methods: a guide to modern techniques of plant analysis. New York: Chapman and Hall.Kadota, S., Marpaung, L., Tohru, K., and Ekimoto, H. 1990. Constituents of the seeds of Switenia mahagoni JACQ. I. isolation, structures, and 1H- and 13C-nuclear magnetic resonance signal assignments of new tetranorterpenoids related to swietenine and swietenolide. Che. Pharm. Bull. 38(3): 639–651.Rahman, A. K. M. S., Chowdhury, A. K. A., Ali, H.A., Raihan, S.Z, Ali, M.S., Nahar, L., and Sarker, S.D. 2009. Antibacterial activity of two limonoids from Swietenia mahagoni against multiple-drug-resistant (MDR) bacterial strains. J Nat Med. 63: 41–45.Yadav, L.D.S. 2005. Organic spectroscopy. Allahabad: Kluwer Academic Publishers.Dewanjee, S., Maiti, A., Das, A.K., Mandal, S.C., and Dey, S.P. 2009. Swietenine: a potential oral hypoglycemic from swietenia macrophylla seed. Fitoterapia. 80: 249–251.Maiti, A., Dewanjee, S., and Sahu, R. 2009. Isolation of hypoglycemic phytoconstituent from Swietenia macrophylla Seeds, Phytother. Res. 23: 1731–1733.Marliana, S.D., Suryanti, V., and Suyono. 2005. Skrining fitokimia dan analisis kromatografi lapis tipis komponen kimia buah labu siam (Sechium edule Jacq. Swartz.) dalam ekstrak etanol, Biofarmasi. 3 (1): 26-31.Nurhayati, Siadi, K., and Harjono. 2012. Pengaruh konsentrasi natrium benzoat dan lama penyimpanan pada kadar fenolat total pasta tomat, Indo. J. Chem. Sci. Vol. 1 (2): 158-163.Siadi, K. 2012. Ekstrak bungkil biji jarak pagar (Jatropha curcas) sebagai biopestisida yang efektif dengan penambahan larutan NaCl. Jurnal MIPA. 35 (1): 80-81.Klein, D.R. 2012. Organic chemistry. New Jersey: John Wiley Sons.Pavia, D.L., Lampman, G.M., and Kriz, G.S. 2001. Introduction to spectroscopy. London: Thomson Learning.Minaeva, V.A., Minaev, B.F., Baryshnikov, G.V., Romeyko, O.M., and Pittelkow, M. 2013. The FTIR spectra of substituted tetraoxa[8]circulenes and their assignments based on DFT calculations, Vibrational Spectroscopy. 65: 147–158.Mootoo, B.S., Ali, A, Motilal, R, Pingal, R, Ramlal, A, Khan, A, Reynolds, W.F, and McLean, S. 1999. Limonoids from Swietenia macrophylla and S. aubrevilleana. J. Nat. Prod. 62: 1514-1517.Lin, B.D., Yuan, T., Zhang, C.R., Dong, L., Zhang, B., Wu, Y., and Yue, J.M. 2009. Structurally diverse limonoids from the fruits of Swietenia mahagoni. J. Nat. Prod. 72: 2084–2090.Cheng, Y.B., Chien, Y.T., Lee, J.C., Tseng, C.K., Wang, H.C., Lo, W., Wu, Y.H., Wang, S.Y., Wu, Y.C., and Chang, F.R. 2014. Limonoids from the seeds of Swietenia macrophylla with inhibitory activity against dengue virus 2. J. Nat. Prod. dx.doi.org/10.1021/np5002829.Zhang, W.M., Liu, J.Q., Deng, Y.Y., Xia, J.J., Zhang, R.N., Li, Z.R., and Qiu, M.H. 2014. Diterpenoids and limonoids from the leaves and twigs of Swietenia mahagoni. Nat. Prod. Bioprospect. 4:53–57.Ma, Y.Q., Jiang, K., Deng, Y., Guo, L., Wan, Y.Q., and Tan, C.H. 2017. Mexicanolide-type limonoids from the seeds of Swietenia macrophylla, Journal of Asian Natural Products Research, DOI: 10.1080/1028-6020.2017.1335715.Zang, Y., amd Xu, H. 2017. Recent progress in the chemistry and biology of limonoids. RSC Adv. 7: 35191–35220.Tan, Q.G., and Luo, X.D. 2011. Meliaceous limonoids: chemistry and biological activities. Chem. Rev., 111, 7437–7522.Fang, X., Di, Y. T, and Hao, X. J. 2011. The advances in the limonoid chemistry of the Meliaceae family. Current Organic Chemistry. 15: 1363-1391.Taylor, D. A. H. The chemistry of the limonoids from meliaceae. https://link.spri-nger.com/book-series/126. Accessed on 21 July 2017.Zang, Y., amd Xu, H. 2017. Recent progress in the chemistry and biology of limonoids. RSC Adv. 7: 35191–35220.
The Classification of “Program Sembako” recipients in Payobasung West Sumatra based on the K-nearest neighbors classifier HAZMIRA YOZZA; NINDI MAULA AZIZAH; LYRA YULIANTI; IZZATI RAHMI
Jurnal Natural Volume 23 Number 2, June 2023
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24815/jn.v23i2.29738

Abstract

The "Sembako Program" is a program carried out by the Indonesian government to improve the welfare of low-income communities. The purposes of this study are: (a) to determine the classification of households that deserve to receive basic-food assistance in Koto Panjang Payobasung, West Sumatra, using the KNN classifier and (b) to determine the optimal number of nearest neighbors used in the classification process. The measure of proximity between objects used is the Gower dissimilarity coefficient. This research used primary data consisting of 175 households collected purposively in a survey conducted on all households in Payobasung.  The optimal K value is determined by implementing a 5-fold cross-validation procedure. The result showed that the best classification process is when K = 3 nearest neighbors are used since it produces the highest accuracy coefficient and Mattews correlation coefficient (MCC). Therefore, for further work, in deciding the eligibility of a household to receive the Sembako Program in Payobasung, KNN can be used by considering its 3 nearest neighbors
THE CYTOTOXIC ACTIVITY OF N-HEXANE EXTRACT OF KERSEN(muntingia calabura linn.) LEAVES USING THE BRINE SHRIMP LETHALITY TEST (BSLT) METHOD Irma Sari; Titania Miranda; Sadli Sadli
Jurnal Natural Volume 16, Number 2, September 2016
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24815/jn.v16i2.5124

Abstract

The cytotoxic activity experiment of n-hexane extract of kersen (Muntingia calabura Linn.) leaves has been carried out using the Brine Shrimp Lethality Test (BSLT) method. The purpose of this research is to identify the secondary metabolites, extract characterize, and determine the LC50 value of the extract against larvae shrimp Artemia salina Leach. Screening result showed that n-hexane extract of kersen leaves contain triterpenoid. Extract characterization showed the results water content of 2.59±0.18 %w/w, water soluble extractive content of 1.9%±0.19 %w/w, the ethanol soluble extractive content of 17.41±1,87 %w/w and total ash value 0.25% w/w. Extract showed LC50 value is 278,72 ppm were calculated by probit analysis. The LC50 value indicated that the n-hexane extract of kersen leaves potentially has cytotoxic activity.

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