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SAINS MEDIKA : JURNAL KEDOKTERAN DAN KESEHATAN

sainsmedika
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Published by Universitas Islam Sultan Agung

ISSN : 20851545 EISSN : 2339093X DOI : -

Core Subject : Health,

Health Professions Medicine & Pharmacology

Sains Medika is journal of medicine and health, is a peer-reviewed and open access journal that focuses on promoting medical sciences generated from Biomedical Sciences, Public Health, Clinical Sciences, and Medical Education to integrate researches in all aspects of human health. This journal publishes original articles, reviews article, and also interesting case reports.

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Articles 7 Documents

Search results for , issue "Vol 11, No 1 (2020): June 2020" : 7 Documents clear

The Influence of Continuous Multi Stage Countercurrent Extraction Process (CMCE) Propolis Extract Administration on Superoxide Dismutase Activity and Hepatocytes Fibrosis Degree (An Experimental Study on Male Wistar Rats Induced with Carbon Tetrachloride (CCl4)) Wani, Elsayanti; Nasihun, Taufiqurrachman; Hussaana, Atina
Sains Medika: Jurnal Kedokteran dan Kesehatan Vol 11, No 1 (2020): June 2020
Publisher : Faculty of Medicine, Universitas Islam Sultan Agung (UNISSULA), Indonesia

Introduction: Continuous Multi Stage Countercurrent Extraction Process (CMCE) propolis extract containing Caffeic Acid Phenethyl Ester (CAPE) may inhibit the formation of lipid peroxidation. However, it is still unclear whether or not CMCE propolis is able to increase SOD activity and reduce acute hepatocytes damages induced by CCl4. Objective: To evaluate the influence of CMCE propolis extract administration in increasing the SOD activity and reducing the hepatocytes fibrosis of male Wistar rats induced with CCl4.Method: an experimental research with a post-test only control group design. Twenty eight male Wistar rats were divided into four groups. Group C-N was only induced with CCl4, while group CM-3, CM-7, and CM-14 were induced with CCl4 and received CMCE propolis extract respectively with 3.6 mg/200 g BW, 7.2 mg/200 g BW, and 14.4 mg/200 g BW. CMCE propolis was administered for 14 days and then CCl4 was administered on the last day. SOD activity was measured using the colorimetric method, while the hepatocytes fibrosis degree was assessed using Image J Software method with NASH score modification.Results: Post Hoc LSD analysis indicated that SOD activity in group CM-3 (51.67 Â± 4.20), CM-7 (70.21 Â± 6.26), CM-14 (80.85 Â± 4.59) was lower than that in group C-N (23.70 Â± 5.12) with P <0.05. Meanwhile, the Mann-Whitney U test showed that the hepatocytes fibrosis degree in group CM-3 (0.85 Â± 0.69), CM-7 (1.14 Â± 0.37), and CM-14 (0.71 Â± 0.48) was significantly lower than that in group C-N (2.28 Â± 1.25) with p<0.05.Conclusion: The CMCE propolis extract administration was able to increase the SOD activity and reduce the hepatocytes fibrosis degree of male Wistar rats induced with CCl4

The Immunomodulatory Effect of Cinnamon (Cinnamomum Burmanii) Bark Extract On the C-Reactive Protein (CRP) Level, Leukocyte Count and Leukocyte Type Count of Wistar Rats Exposed to Staphylococcus Aureus Utomo, Astika Widy; Retnoningrum, Dwi; Gumay, Ainun Rahmasari
Sains Medika: Jurnal Kedokteran dan Kesehatan Vol 11, No 1 (2020): June 2020
Publisher : Faculty of Medicine, Universitas Islam Sultan Agung (UNISSULA), Indonesia

Introduction: Bacterial infection induces inflammation in human body. This process produceshumoral and cellular immune responses. Cinnamomum burmaniigrows very vast in Indonesia and contains cinnamaldehyde known to have an anti-inflammatory effect.Objective: To prove the effect of C. burmanii bark extract on CRP level, leukocyte count and differential blood count.Methods:Â Aposttest-only controlled group design with 25 Wistar Rats divided into 5 groups was employed. The CN-G group was giventhe standard feed, the CP-G group was given the standard feed and levamisole 2.5 mg/KgBW, while the CBE-100, CBE-200, and CBE-400 groups were respectively given the standard feed and cinnamon bark extract 100 mg/kgBW, 200 mg/KgBW and 400 mg/KgBW. The treatmentswereconducted for 7 consecutive days.On day 8, all rats were injected with the suspense of S. aureus intraperitoneally. The blood wasthen drawn on day 9, followed with CRP level measurement using the ELISA method. The total leukocyte count and differential blood count weremanually measured.Results:Â There is no significant difference in the value of CRP level (One Way ANOVA; p = 0.749) with the total counts of leukocytes(p=0.685), monocytes (p=0.769), and eosinophil(p=0.123) between groups. The neutrophils and lymphocytes of CBE-100 group aresignificantly differentfrom the other groups.Conclusion:Â C. burmanii extract has a potential benefit as immunomodulator.Â

The Effect of Honey in Second Degree Burn Healing on Wistar Rats (Overview of Angiogenesis and the Number of Fibroblasts) Almaz, Ayyasi Izaz; Purnawati, Ratna Dama; Istiadi, Hermawan; susilaningsih, Neni
Sains Medika: Jurnal Kedokteran dan Kesehatan Vol 11, No 1 (2020): June 2020
Publisher : Faculty of Medicine, Universitas Islam Sultan Agung (UNISSULA), Indonesia

Introduction: Burn therapy is generally done using a burn ointment. However, honey can also be used as an alternative treatment for burns. Honey contains active flavonoid as an antioxidant and hydrogen peroxide which can stimulate fibroblasts proliferation also angiogenesis on burn healing phase.Objective: to determine the effect of honey in second degree burn healing from angiogenesis and the number of fibroblasts on Wistar rats. Methods: This research used a posttest only controlled group design on 15 rats with random selection and divided into 3 groups: H-G was given honey, B-G was given BioplacentonÂ®, N-G without any treatment after burn was induced. Rats were given a second degree burn in a size of 2 cm x 2cm on the back area and were treated twice a day for 14 days. Burn healing was measured by calculating the observation results of microscopic VEGF expression, the number of neovascularization, and the number of fibroblasts. Hypothesis analysis used Annova post hoc LSD and Mann-Whitney.Results: The highest mean in VEGF expression and the number of neovascularization was obtained from group B-G followed by group H-G and group N-G. Significant differences in the number of neovascularization between group B-G with group N-G, p<0.05.Conclusion: Honey can provide a healing effect on the second degree of burns in Wistar rats in terms of the number of neovascularization as well as BioplacentonÂ®

The Effect of CMCE Propolis Extract Administrations on Interleukin-1 (IL-1) Levels and the Hepatocyte HistopathologicalFindings of Ratsâ€™ Induced with CCl4 Lestari, Catur Retno; Sumarawati, Titiek; Nasihun, Taufiqurrachman
Sains Medika: Jurnal Kedokteran dan Kesehatan Vol 11, No 1 (2020): June 2020
Publisher : Faculty of Medicine, Universitas Islam Sultan Agung (UNISSULA), Indonesia

Introduction:Liver exposure to toxic substances will disrupt the metabolic system. Carbon tetrachloride (CCl4) is a hepatotoxic substance causing a liver damage. Continuous multi-stage countercurrent extraction(CMCE) propolis extract contains active balsamic substances, caffeic acid, phenethyl esters and flavonoids.Objective: to prove the effect of CMCE propolis extract on IL-1 levels and the hepatocyte histopathology findingsof male wistar ratsâ€™induced by CCl4.Methods:In the posttest only control group design, the total sample of 28 rats was divided into 4 groups: positive control group (K-0; standard feed); group P-1 (propolis3.6 mg/200 g); P-2 (propolis7.2mg/200gr and P-3 (propolis14.4mg/200g) were respectively given for 14 days,yet those on the 14thday were given CCl4. The IL-1 level was measured with the ELISA method, while the male wistar ratsâ€™hepatocyte histopathology findings was determined by the HE staining methods. IL-1 levelswere analyzed usingOne Way Anova test,followed by the Post Hoc LSD test. Hepatic cell histopathology was analyzed usingKruskal Wallis, followed by Mann-Whitney U.Results:Post Hoc LSD analysis shows that the levels of IL-1 in P-1 (983.54 Â±19.67), P-2 (841.58Â±19.47), and P-3 (755.50Â±31.63) are significantlylower thanthosein K-0 (1131.69Â±14.91) withp<0.05. Mann-Whitney U analysis shows that the hepatocyte histopathology in the P-1 group (2.55Â±0.39), P-2 (1.24Â±0.94) and P-3 (1.20Â±0.93) is significantlylower thanthatinK-0 (2.94Â±0.16) with p<0.05.Conclusion: The administration of CMCE propolis extract may reduce IL-1 levels and improve the male wistar ratsâ€™hepatic cell histopathologyinduced with carbon tetrachloride (CCl4).

Exploration of Xanthone Derivatives as Anti-Cancer Agents against Colorectal Cancer Miladiyah, Isnatin
Sains Medika: Jurnal Kedokteran dan Kesehatan Vol 11, No 1 (2020): June 2020
Publisher : Faculty of Medicine, Universitas Islam Sultan Agung (UNISSULA), Indonesia

Colorectal cancer currently occupies the third position of globally cancer morbidity and is the second leading cause of cancer death after lung cancer. Chemotherapy administration is still the main modality for the colorectal cancer stage I to III. Its usage is limited, since its high resistance and risk of side effects. Thus, exploration and development of novel colorectal anti-cancer drugs, including compounds derived from nature origin is needed. Xanthone becomes one natural compound which may be potentially developed as an anti-colorectal cancer due to its cytotoxic and anti-proliferative activities. The in vitro cytotoxic and anti-proliferative actions of xanthone compounds occur through a variety of mechanisms involving both apoptotic induction and inhibition of cell cycle, while in vivo may reduce the tumor size. Since colorectal cancer is frequently treated in a combination therapy, xanthone compounds have also been studied in a combination use and proven effective as co-chemotherapy with the standard chemotherapy drugs. This paper aims at providing an overview of colorectal cancer, pathology, risk factors and protective factors, as well as discussing the current therapies and potential xanthone compounds as an alternative therapy which may be developed later for the colorectal cancer

Invitro Antioxidant and Cytotoxic Potential of Ficus carica L. and Olea europeae L. Against Cervical Cancer Widyaningrum, Naniek; Hussaana, Atina; Puspitasari, Nesya Ayu
Sains Medika: Jurnal Kedokteran dan Kesehatan Vol 11, No 1 (2020): June 2020
Publisher : Faculty of Medicine, Universitas Islam Sultan Agung (UNISSULA), Indonesia

INTRODUCTION:Cervical cancer is a malignant infectious disease caused by Human Papilloma Virus (HPV) in the cervix. Fig and olive oil containing flavonoid has been shown to have antioxidant and anticancer activity.OBJECTIVE:This study aimed to evaluate antioxidant and cytotoxic activity of combined Ficus carica L. and Olea europeae L. against HeLa cervical cancer cells.METHODS: This is an experimental study with a post-test only control group design. The HeLa cells were divided into 5 groups: fig extract, olive oil, combined fig extract and olive oil (at the ratio of 1:3, 1:1, 3:1), positive control (doxorubicin). The cytotoxic and antioxidant activity were evaluated by using MTT Assay and DPPH, respectively. The cytotoxic results were analyzed using probit and antioxidant activity was analyzed by using linear regression to obtain IC50 values.RESULTS:The IC50 cytotoxic of fig extract, olive oil, combined fig extract and olive oil (at the ratio of 1:3, 1:1, 3:1) with positive control (doxorubicin) were 13063,915 Î¼g/mL, 679,593 Î¼g/mL, 1562,356 Î¼g/mL, 746,923Î¼g/mL, 563,626 Î¼g/mL and 13,707 Î¼g/mL respectively. The IC50 antioxidant of fig extract, olive oil, and combination of fig extract and olive oil (3:1) was 105.9272 ppm, 23.1276 ppm, and 21,0689 ppm respectively.CONCLUSION:The combination of fig extract and olive oil (3:1) was shown to have the highest antioxidant and cytotoxic activity against HeLa cervical cancer cells.

The Difference between the Effect of Green Tea Cream and Tocopherol on Decreasing Level of Tyrosinase Enzyme and Amount of Melanin in Rattus norvegicus Exposed to UVB Rays Riyanto, Anwar; Nasihun, Taufiqurrachman; Sumarawati, Titiek
Sains Medika: Jurnal Kedokteran dan Kesehatan Vol 11, No 1 (2020): June 2020
Publisher : Faculty of Medicine, Universitas Islam Sultan Agung (UNISSULA), Indonesia

Introduction: Green tea and tocopherol are potent antioxidants used to treat melasma. However, whether green tea or tocopherol is superior remains unclear. Objective: To compare the effectiveness between green tea and tocopherol in decreasing the amount of melanin and tyrosinase level in wistar rats exposed to UVB rays.Methods: The experimental study took 30 male Wistar rats randomly and divided them into 3 groups. The control group (C-G) was given basic topical cream, GT-G was given green tea topical cream and TC-G was given tocopherol topical cream. All rats were exposed to UVB every Monday, Wednesday and Friday for 4 weeks, whereas topical creams were smeared every day. Topical creams smearing on the same day with UVB exposure was performed 20 minutes before exposure and 4 hours after UVB exposure. The doses of UVB were 50 mJ/cm2 in first week, 70mJ/cm2 in the second week and 80mJ/cm2 in the third and fourth weeks. The amount of melanin was measured using pixel method and the tyrosinase level was measured using ELISA.Results: Anova analysis indicates that the amount of melanin and tyrosinase level are significantly different between groups, p<0.05. Post Hoc LSD analysis indicates that the amount of melanin in GT-G and TC-G are significantly lower than that of C-G, p<0.05. The amount of melanin in GT-G is lower than that of TC-G, p<0.05. The tyrosinase level in GT-G is significantly lower than that of C-G and TC-G, p<0.05. Meanwhile, the tyrosinase level in TC-G is lower than that of C-G but insignificantly, p>0.05.Â Conclusion: Green tea topical treatment is significantly capable of decreasing the amount of melanin and tyrosinase level better than tocopherol.

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