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I Wayan Suardana
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Journal of Veterinary and Animal Sciences
Published by Universitas Udayana
ISSN : 25501283     EISSN : 26220571     DOI : -
Core Subject : Health,
Veterinary
It covers all the scientific and technological aspects of veterinary medicine in general, anatomy, physiology, biochemistry, pharmacology, microbiology, pathology, public health, parasitology, infectious diseases, clinical sciences, biotechnology, alternative veterinary medicine and other biomedical fields. In the field of animal science, the journal receives original manuscripts covering breeding and genetics, reproduction and physiology, nutrition, feed sciences, animal products, biotechnology, behavior, livestock farming system, socio-economic, and policy.
Arjuna Subject : Kedokteran Hewan - Kedokteran Hewan (Lain-Lain)
Articles 78 Documents
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Molecular Analysis of Lactic Acid Bacteria SR4 Strain Isolated from Gastric’s Juice of Bali Cattle Iwan Harjono Utama; I Wayan Suardana
Journal of Veterinary and Animal Sciences Vol 4 No 2 (2021)
Publisher : Institute for Research and Community Service, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JVAS.2021.v04.i02.p03

Abstract

Lactic acid bacteria SR4 strain is known to have potential as a probiotic source according to the previous study. The isolate derived from the gastric’s juice of bali cattle has characteristics such as its resistance to growth at low pH, namely pH 2, 3, and 4, its tolerance to bile concentration of 0.6 mM, and its inhibition against Gram-positive and negative bacteria. On the other hand, a complete molecular analysis of the isolate has never been carried out. Based on these considerations, the molecular analysis of the LAB SR4 strain becomes interesting to present. The study was initiated by cultivating LAB SR4 isolate on an MRS broth medium. The isolate was further confirmed molecularly through analysis of the 16S rRNA gene which was initiated by isolating genomic DNA followed by PCR reaction using universal primers 27F and 1492R. The PCR product of the 16S rRNA gene was sequenced and further analyzed using the MEGA 5.2 software. The data were then analyzed descriptively and presented in the form of tables or figures. The results showed that the LAB strain SR4 was identified as a Pediococcus pentosaceus. This strain potentially to develops as a source of bacteriocin and it can be applied as a bio preservative, improvement of the meat taste, inhibition of inflammation, and also as antagonism of cancer.
Studies on Lactic Acid Bacteria Isolate Sr 13 From Bali Cattle Gastric Bagus Nanda Govinda Muria Siddhi; I Wayan Suardana; Nyoman Semadi Antara
Journal of Veterinary and Animal Sciences Vol 2 No 1 (2019)
Publisher : Institute for Research and Community Service, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JVAS.2019.v02.i01.p05

Abstract

Lactic acid bacteria (LAB) is a generic term for bacteria that ferment lactose and produce lactic acid as its main product. In general, lactic acid bacteria belonging to Gram positive bacteria, negative catalase, does not form spores, have no cytochrome, aerotolerant, anaerobic to microaerophilic, require complex nutrients such as amino acids, vitamins (B1, B6, B12 and biotin), purine, pyrimidine. This study aims to determine the species of lactic acid bacteria (LAB) from isolate SR13 isolated from rumen liquid of bali cattle. The identification was started by cultivation of isolate on de Man, Rogosa, Sharpe (MRS) medium with anaerobic condition, confirmation of isolates by Gram staining and catalase test, and finally identificion of species by using conventional test and 50CH API test kit. The results of study showed, the isolate of LAB SR 13 was detected asPediococcus/Enterococcus, moreover, identification by using 50CH Api test kit showed as Pediococcus pentosaceus 2
Cover, Editorial Board, and Table of Content Journal of Veterinary and Animal Science
Journal of Veterinary and Animal Sciences Vol 3 No 2 (2020)
Publisher : Institute for Research and Community Service, Udayana University

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Abstract

Cover, Editorial Board, and Table of Content
Seroprevalence of Newcastle Disease in Kampong Chickensin Gianyar Regency Bali Gusti Ayu Yuniati Kencana; I Made Kardena; Ni Putu Eka Hari Andini
Journal of Veterinary and Animal Sciences Vol 1 No 2 (2017)
Publisher : Institute for Research and Community Service, Udayana University

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Abstract

Newcastle disease (ND) or Tetelo is one of the viral diseases in poultry that causes high mortality and significant economic losses in chicken farms. Newcastle Disease is endemic in Bali as well as in Gianyar. Kerta village is one of the villages in Payangan Subdistrict at Gianyar regency where its location is nearby to the centre of hi breed chicken breeding industry in Kintamani Subdistrict at Bangli Regency and is the poultry trade traffic way from Kintamani to Denpasar. This study aimed to determine the seroprevalence of ND in kampong chickens in the village of Kerta at Gianyar regency. Purposive serum samples were collected from 80 unvaccinated backyard chickens from four (4) banjar (Kerta, Pilan, Buhu, and Marga Tengah) in Kerta village. Serological tests using Hemagglutination (HA) and Hemagglutination Inhibition (HI) were performed to the sera samples. Twenty out of the 80 serum samples (25%) were ND positive. The proportion of ND seropositive in each banjar was: 35%, 30%, 30% and 5% at Kerta, Pilan, Buhu, and Central Marga banjar, respectively. Twelve out of the 20 seropositive samples (12/20 = 60%) had protective antibodies, whilst the remains were negative. It is concluded that the seroprevalence of ND disease in the kampong chickens in Kerta village was 25% with antibody titres of 22 to 29 HI units which derived from natural infection.
The Change in External Genital and Estrogen Level of Bali Cattle During Estrus Desak Nyoman Dewi Indira Laksmi; I Gusti Ngurah Bagus Trilaksana
Journal of Veterinary and Animal Sciences Vol 3 No 1 (2020)
Publisher : Institute for Research and Community Service, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JVAS.2020.v03.i01.p05

Abstract

The successful application of reproductive technology requires knowledge of estrus cycle and the ability to detect estrus of Bali cattle. Estrus in cattle lasts for 8-30 hours showed by the acceptance of males for copulation. In this phase, estrogen levels reach maximum levels. High levels of estrogen cause signs of estrus, and changes in the reproductive organs. This study aims to determine estrogen levels during estrus and external genital changes during estrus. This research was an observational analytic study with cross-sectional study design. The samples used in this study were Bali cattle during estrus. The samples had healthy status without any signs of disease. Blood samples were taken through the jugular vein were then centrifuged to obtain serum. The obtained serum was examined for estrogen hormone levels. Mechanical measurements of hormone levels checked by using the Direct Elisa, Double Antibody Sandwich. Blood samples were collected from several SIMANTRI in Mengwi District, Badung Regency. The results showed in Bali cattle external genitals change when estrus, namely redness of the vaginal mucosa, discharge from the vagina and swelling of the vulva. While estrogen levels when estrus is 69.80 pg/ ml. Further research needs to be continued to measure the quality of ovulation after estrus.
Herbal Extract as An Antibacterial Against Gram Positive Bacteria Causing Dermatitis Complex I Nyoman Suartha; I GustiKetut Suarjana; Luh Made Sudimartini; I Made Merdana; I Made Dira Swantara
Journal of Veterinary and Animal Sciences Vol 1 No 1 (2017)
Publisher : Institute for Research and Community Service, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JVAS.2017.v01.i01.p07

Abstract

Study in order to evaluate the antibacterial effect of herbal extract from leaves of three different species of plant namely neem(Azadirachtaindica A. Juss), gotu-kola (Centellaasiatica), andsoursop (Annona muricata L) against bacteria causing dermatitis complex in dogs have been undertaken. The antibacterial activities of the herbal extract were evaluated using the agar diffusion test with extract at three different concentrations 5%, 10%, and 25%, respectively.Staphylococcus aureus was isolated from the dermatitis complex cases in dogs. The results showed various antibacterial activities of all the herbal extract at all concentration as indicated by the diameter of inhibition haloes produced around the orifice. The average inhibition haloes for each herbal extract concentration was 2.19± 0.53 mm; 4.32± 0.84 mm; 5.86± 0.79 mm, respectively. It can be concluded that herbal extract from the three plants is a potential antimicrobial against agents causing dermatitis complex.
Case Report: Helminths Infestation with Bronchopneumonia and Tick Infestation in Mixed Dog Ni Luh Mentari Saavitri Nesa; Putu Ayu Sisyawati; I Gusti Made Krisna Erawan
Journal of Veterinary and Animal Sciences Vol 6 No 1 (2023)
Publisher : Institute for Research and Community Service, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JVAS.2023.v06.i01.p05

Abstract

Abstract. A mixed-breed dog named Vino, male, golden brown, two years old, weighing 10 kg was brought to the Veterinary Internal Medicine Laboratory, Faculty of Veterinary Medicine, Udayana University with a non-productive cough four months before the examination. Two weeks after the onset of the cough, the dog showed signs of itching. There were blood spots in the feces and the consistency is very soft but still has shape. Respiratory examination revealed that the case dog had a non-productive cough. Auscultation of the lungs revealed deep breathing and slight crackles. Skin examination revealed Rhipicephalus sp. Infestation with ulcerative lesions, erythema, papules, hyperpigmentation, lichenification, and alopecia. On radiography, there was a bronchial pattern and radiopaque spots in the lungs. The results of the complete blood count showed leukocytosis, mild neutrophilia, eosinophilia, and mild hypochromia. Stool examination revealed the presence of Ancylostoma spp. and Strongyloides spp. eggs. Examination of the impression smear and tape acetate preparation test with cytologic staining revealed some neutrophils. The case dog was diagnosed with Ancylostoma spp. and Strongyloides spp. infestation accompanied by bronchopneumonia and tick infestation. Treatment was administered with Caniverm®, Advocate®, and methylprednisolone. After 14 days of treatment, the dog's condition improved, as evidenced by no worm eggs found in the feces and the case dog was no coughing or scratching by the case dog, and no ticks found on the case dog's body.
Complete Blood Profile and Neutrophil-Lymphocyte Ratio of Electrical Stunning and Non-Stunning Swine Bq Nurlita Anugrah; I Wayan Suardana; Hamong Suharsono
Journal of Veterinary and Animal Sciences Vol 5 No 2 (2022)
Publisher : Institute for Research and Community Service, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JVAS.2022.v05.i02.p04

Abstract

Examination of the blood profile is crucial because blood functioned as an important role in the circulatory system and can be used to evaluate the emergence of stress in animals. The purposes of this research are to examine the complete blood profile and examine the possibility of stress symptoms by measuring the ratio of neutrophils-lymphocytes as the indicator of stress in electrical stunned and non-stunned swine. There were 40 six-month life swine with a weight of 80-100kg. The swine grouped into stunning and non-stunning group. A total of 3 ml of blood sample occupied and placed to a contained anticoagulant Ethylene Diamine Tetraacetic Acid (EDTA) vacutainer. The total of erythrocytes, hemoglobin, PCV, MCV, MCH, leukocytes and differential leukocytes were calculated using routine hematological examination methods with autoanalyzer machines. Based on the results of the research, the complete blood profile of electrical stunning slaughtered swine was healthier and more reliable than non-stunning. The ratio of neutrophils-lymphocytes in electrical stunning slaughtered swine was lower than non-stunning. This condition indicated a lesser amount of stress on the use of electric stunning.
Selection of Bali Cattle as a Bull in Nusa Penida Island-Bali Based on Its Performance and Breeding Value Dewi Ayu Warmadewi; I Nyoman Ardika; I Gusti Agung Arta Putra
Journal of Veterinary and Animal Sciences Vol 2 No 2 (2019)
Publisher : Institute for Research and Community Service, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JVAS.2019.v02.i02.p03

Abstract

Selection program applied to improve the animal genetic potential. This program can be carried out based on the performance and breeding values ??of the economic trait of each individual. The research was carried out for 5 month (from May until September 2018). The purpose of this study was to find out the superior Bali bull by determining of chest girth male Bali cattle. This study used purposive random sampling method. The number of male Bali cattle with incisor of I0,I1,I2 used in this study were 147,29,19 heads respectively. Performance test was calculated by formula of BV = h2 ( Pi – P ) (Hardjosubroto, 1994). The results showed that the average of chest girth male Bali Cattle for I0, I1 and I2 were 135,65±16,98cm; 159,57±13,75cm and 166,26±19,94cm respectively. Based on the breeding value, male Bali cattle coded BK030 with I1 and BK101 with I2 were recommended as a superior Bali bulls because of their highest ranking.
Characteristics of Rumen Fluid, pH and Number of Microbia I Gusti Ketut Suarjana; Ketut Tono Pasek Gelgel; Putu Henrywaesa Sudipa
Journal of Veterinary and Animal Sciences Vol 4 No 1 (2021)
Publisher : Institute for Research and Community Service, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JVAS.2021.v04.i01.p02

Abstract

Abstract. This research purpose is to determine the characteristics of pH rumen fluid and the amount of microbes in the rumen of Bali cattle. The research sample is healthy bali cattle rumen fluid which is slaughtered at Pesanggaran slaughterhouse Denpasar with a total of 30 samples. The research method uses conventional methods and refers to the method according to [6]. The total population of bacterial and fungal colonies was calculated by the pour plate method. Bacterial colonies were cultured on nutrient agar plates and fungi colonies were cultured on sabouraud dextrose agar plates. The Population of Enterobacteriaceae bacterial colonies was calculated by scatter method on Mac Conkey agar plate and Escherichia coli colony on Eosin Methylen Blue agar plate. Rumen liquid pH measurement is directly done with pH indicator strips. The study was conducted observationally and the data obtained were analyzed descriptively. The results showed that the total number of bacteria in the rumen fluid of Bali cattle with an average of 64x104 cfu/g, the number of fungal colonies with an average of 161x105 cfu/ g, the number of Coliform colonies was 23x104 cfu/g, the number of non-Coliform 28x103 cfu/g, E.coli 18x103 cfu/g and the mean The pH of Balinese cattle rumen fluid 6.9. Keywords : Balinese Cattle Rumen Fluid, Coliform, E. Coli , Fungi, non-Coliform, Total Bacteria , pH

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