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INDONESIA
Jurnal Bioteknologi & Biosains Indonesia (JBBI)
Published by Badan Pengkajian dan Penerapan Teknologi
ISSN : 24422606     EISSN : 2548611X     DOI : -
Core Subject : Health, Science, Agriculture, Social,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Health Professions
JBBI is published twice annually and provide scientific publication medium for researchers, engineers, practitioners, academicians, and observers in the field related to biotechnology and bioscience. This journal accepts original research papers, review articles, case studies, and short communications. The articles published are peer-reviewed by no less than two referees, and cover various biotechnology subjects related to the field of agriculture, industry, health, environment, bioinformatics, as well as life sciences in general.
Arjuna Subject : Umum - Umum
Articles 177 Documents
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IN SILICO EVALUATION OF FLAVONOIDS FROM ACALYPHA INDICA L. AS Α-GLUCOSIDASE INHIBITORS FOR THE TREATMENT OF DIABETES MELLITUS Lisa Savitri; Kharisul Ihsan; Fendy Prasetyawan; Yuneka Saristiana; Imam Agus Faizal; Meri Meri
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 13 No. 1 (2026)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

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Abstract

Type 2 diabetes mellitus continues to pose a significant global health burden, highlighting the urgent need for safer and more effective therapeutic agents with minimal adverse effects. One promising strategy involves targeting α-glucosidase, a key enzyme in postprandial glucose regulation, using bioactive compounds derived from medicinal plants. Acalypha indica, a tropical species widely used in traditional medicine, contains diverse flavonoids with potential antidiabetic activity; however, their molecular mechanisms remain insufficiently explored. This study employed an in silico approach to systematically evaluate the α-glucosidase inhibitory potential of five flavonoids from A. indica—mauritanin, repandusinic acid, hesperetin, glucogalin, and acaindinin. Molecular docking analysis using AutoDock revealed that all compounds exhibited favorable binding affinities toward the α-glucosidase enzyme (PDB ID: 5NN8), indicating spontaneous interactions at the active site. Among them, mauritanin demonstrated the highest binding affinity (–10.3 kcal/mol), forming multiple stabilizing hydrogen bonds with critical catalytic residues, including ASP69, HIS279, and GLU411. Notably, both mauritanin and repandusinic acid showed stronger binding interactions compared to the standard inhibitor acarbose, suggesting superior inhibitory potential. Further interaction analysis using LigPlot+ and Discovery Studio Visualizer confirmed stable ligand–enzyme complex formation, while ADMET predictions using SwissADME indicated favorable pharmacokinetic properties, including good oral bioavailability, low toxicity risk, and absence of major cytochrome P450 inhibition. Overall, these findings identify mauritanin and repandusinic acid as promising lead compounds for α-glucosidase inhibition and support the therapeutic potential of A. indica as a natural source for antidiabetic drug development. This study provides a strong computational foundation for future experimental validation and drug design efforts.
ANALYSIS OF L-Asparaginase II ENZYME ACTIVITY FROM NATIVE AND RECOMBINANT Escherichia coli DH10B FOR THE APPLICATION OF ACUTE LYMPHOBLASTIC LEUKAEMIA THERAPY Keysha Putri Febiona; Dian Pertiwi; Arni Amir; Lily Syukriani; Dessy Arisanty; Almurdi Almurdi; Lisana Shiddiqin Aliya; Jamsari Jamsari
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 13 No. 1 (2026)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

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Abstract

Acute Lymphoblastic Leukemia (ALL) is a blood cancer that occurs due to abnormal proliferation of lymphoid progenitor cells. L-asparaginase II is an enzyme used to treat this disease by inducing apoptosis in cancer cells encoded by the AnsB gene. Previous studies have shown complications and toxicity in commercial L-asparaginase, necessitating exploration of L-asparaginase sources. Serratia plymuthica UBCF_13 is one of the microorganisms that encodes the AnsB gene. This study consisted of inoculation of native E. coli DH10B and E. coli DH10B pET28a(+)_AnsB, molecular validation, enzyme activity testing using direct nesslerisation method, and analysis of extracellular and intracellular protein profiles using SDS-PAGE. Enzyme activity and protein profiles were compared with S. plymuthica UBCF_13, and Isopropyl β-D-1-thiogalactopyranoside (IPTG) induction was performed on each bacteria as a comparison. The results showed the highest L-asparaginase II enzyme activity in E. coli DH10B pET28a(+)_AnsB induced with IPTG with an average enzyme activity of 0.392 ± 0.017 U/mL. Protein profile analysis using SDS-PAGE supported this finding, showing a thicker 36.5 kDa protein band compared to other bacteria. Statistical testing using One Way ANOVA showed a significant difference in L-asparaginase II enzyme activity in native E. coli DH10B and E. coli DH10B pET28a(+)_AnsB. It can be concluded that there was an increase in activity in recombinant E. coli DH10B compared to its native.
ASSOCIATION BETWEEN SMOKING STATUS AND DIAGNOSED HEART DISEASE AMONG OLDER ADULTS IN INDONESIA: EVIDENCE FROM THE 2023 NATIONAL HEALTH SURVEY Yuni Asri; Dian Pitaloka Priasmoro; Sasmiyanto Sasmiyanto
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 13 No. 1 (2026)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

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Abstract

Heart disease remains a leading contributor to illness and mortality among older adults globally, including in Indonesia. Although smoking is a well-established cardiovascular risk factor, limited national evidence has explored its relationship with heart disease among the Indonesian elderly. This study utilized data from 97,339 adults aged ≥60 years obtained from the 2023 Indonesia Health Survey (Survei Kesehatan Indonesia/SKI) to examine the association between smoking status and physician-diagnosed heart disease, considering various sociodemographic factors. Key variables included age, sex, education, marital status, place of residence, employment, and smoking behavior. Weighted bivariate analyses were conducted using chi-square tests to account for the complex survey design. The overall prevalence of heart disease was 3.5%. Significant associations (p < 0.05) were observed across all sociodemographic characteristics, including smoking status. Interestingly, heart disease prevalence was higher among non-smokers (2.6%) than among smokers (0.9%), which may suggest reverse causality or smoking cessation following diagnosis. These findings emphasize the influence of sociodemographic and behavioral factors on cardiovascular health among older Indonesians. Longitudinal research is needed to better understand the causal mechanisms underlying these associations, particularly regarding smoking behavior.
SPERM LONGEVITY AND RECOVERY RATE OF BELGIAN BLUE CATTLE IN DIFFERENT EXTENDER Andi Murni Nurul Maulidyah; Muhammad Agil; Syahruddin Said; Taufiq Purna Nugraha; Raden Iis Arifiantini
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 13 No. 1 (2026)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

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Abstract

Frozen semen from Belgian Blue cattle is in high demand among farmers, as evidenced by the increasing interest in this breed's semen. However, the freezing process reduces sperm motility and viability. This study aimed to assess the viability of sperm post-freezing and the sperm recovery rate (RR) in Belgian Blue cattle using different extenders. Semen was collected, evaluated, and diluted in four extenders: three commercially available ones—BoviFree®, AndroMed®,, and Steridyl®,—and a homemade extender, Tris Egg Yolk (TEY). Longevity and RR tests were conducted 24 h after freezing. Progressive motility was assessed using a portable computer-assisted sperm analysis (CASA) device (Androscope Minitube, Germany). The results indicated that sperm motility was higher in the BoviFree®, and AndroMed®, extenders after 3 h compared to the other extenders (p <0.05). After 4 h, motility remained higher in the BoviFree®, extender than in TEY. The RR values showed no significant differences among the extenders (p< 0.05). The study concluded that sperm longevity in Belgian Blue cattle was optimal in the BoviFree®, extender, with consistently good sperm RR values across all extenders.
ANALYSIS OF Deoxyribonucleic Acid (DNA) FRAGMENTATION AND ACROSOME INTEGRITY AS BIOMARKERS OF FROZEN SEMEN QUALITY OF BULLS AT THE SPERM BANK OF SAMARINDA CITY Kirana Dara Dinanti Adiputra; Novemia Fatmarischa; Dani Nur Arifin; Fikri Ardhani; Kumbawan Wibisono; Novi Ramadhani; Erni Damayanti
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 13 No. 1 (2026)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

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Abstract

This study aims to evaluate the quality of frozen bull semen at the Sperm Bank of Samarinda City based on parameters of motility, viability, morphological normality, plasma membrane integrity (PMI), acrosome integrity, and DNA fragmentation, as well as their effects on cattle breeds and storage duration. The samples consisted of frozen semen from five breeds (Limousin, Madura, Angus, Simmental, and Peranakan Ongole) with different storage periods (3, 4, and 5 years). Motility, viability, and morphological normality were tested according to the Indonesian National Standards (SNI). The PMI was assessed using the hypo-osmotic swelling test (HOST), acrosome integrity using peanut agglutinin (PNA) staining, and DNA fragmentation using acridine orange (AO) staining. Data were analyzed using two-way ANOVA, and if there was an effect, it was followed by a Tukey post hoc test. The findings indicated that motility and PMI remained relatively stable; however, the duration of storage significantly influenced PMI (P<0.05). The breed of bull and storage duration had a significant effect on morphological normality, viability, acrosome integrity, and DNA fragmentation (P<0.05). However, the two factors did not interact with each other. It can be concluded that the storage duration resulted in a decline in semen quality, specifically in terms of membrane and DNA integrity. Therefore, it is recommended to use semen straws ≤ 4 years old and conduct periodic quality re-qualification to maintain frozen semen fertility.
DESIGN AND CONSTRUCTION OF A MOBILE-BASED PLANT SEARCH APPLICATION IN THE QURAN WITH FUSE.JS IMPLEMENTATION Fatiya Quzza; Kurnia Saputra; Zumaidar Zumaidar
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 13 No. 1 (2026)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

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Abstract

The availability of applications that specifically integrate plant data in the Quran, related verses, and scientific information is still limited, as indicated by the absence of similar applications on platforms such as the Google Play Store and the App Store. Therefore, this study developed a mobile application supported by a web system for admin data management to provide information on plants in the Quran in a structured and easily accessible manner. The Rapid Application Development (RAD) method was applied for quick iteration and user testing. Data collection found 28 plants mentioned specifically and 11 generally in the Quran. The app displays plant information with related verses and scientific details. A search feature using Fuse.js was added for easier access. Usability testing with UMUX scored 91.53% (“best imaginable”), while black box testing shows the test scenario runs according to the expected results on both platforms.
GLOBAL TRENDS IN PATIENT SAFETY AND SERVICE QUALITY: A BIBLIOMETRIC EXPLORATION FROM CLINICAL PRACTICE TO ORGANIZATIONAL CULTURE Nadila Rahmi; Arlina Dewi
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 13 No. 1 (2026)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

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Abstract

Patient safety and quality of service are crucial indicators in evaluating the effectiveness and accountability of healthcare systems. Despite the widespread implementation of clinical interventions and regulatory measures globally, significant challenges remain in ensuring safe and high-quality services, particularly in developing countries. This study aims to explore the trends and directions of research related to patient safety and quality of service during the 2015–2024 period using a bibliometric approach. Data were retrieved from the Scopus database using the keywords “Patient Safety” AND “Quality of Service” and were analyzed using VOSviewer software to visualize keyword networks, temporal trends, and term density. The analysis identified five major clusters in this area of study: (1) clinical interaction and human factors; (2) safety culture and organizational perception; (3) service quality and clinical procedures; (4) healthcare systems and hospital organization; and (5) the role of institutions and workforce. The findings indicate a shift in research focus from technical approaches toward systemic and humanistic perspectives. Communication among healthcare professionals, organizational culture, and technology adoption emerge as key elements in building an effective and sustainable patient safety ecosystem.
IMPROVING SEMEN QUALITY OF PERANAKAN ONGOLE CATTLE WITH MARIGOLD®-BASED ANTIOXIDANT IN TRIS-EGG YOLK DILUENT Hasan Anwar Hanafi; Abdullah Baharun; Tulus Maulana; Annisa Rahmi; Muhammad Gunawan; Athhar Manabi Diansyah
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 13 No. 1 (2026)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

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This study aimed to determine the optimal concentration of Marigold® antioxidant suplemented in a Tris-egg yolk (TEY) extender to maintain the quality of liquid semen from Peranakan Ongole (PO) bulls. Semen samples were extended in TEY without antioxidant (P0, control) or supplemented with Marigold® extract at concentrations of 0.002% (P1), 0.004% (P2), and 0.006% (P3). Semen quality was evaluated based on macroscopic characteristics (volume, colour, pH, and consistency) and microscopic parameters (mass motility, progressive motility, viability, plasma membrane integrity (PMI), and morphological abnormalities). Fresh semen exhibited an average volume of 5.25±0.8 mL, creamy colour, pH 6.8±0.13, mass motility (++), abnormalities 4.1±0.8%, viability 86.12±6.2%, PMI 77.21±5.3%, and progressive motility 85.44±2.41%. Supplementation with Marigold® antioxidant significantly influenced microscopic parameters during storage at 4 °C. Sperm abnormalities increased with storage duration, particularly in P3 on day 2. Treatment with 0.004% Marigold® (P2) effectively maintained motility and PMI up to day 6, while 0.006% (P3) preserved viability and PMI up to day 10. The addition of Marigold® extract to the TEY extender improved the preservation of PO bull semen quality during 10 days of storage at 4 °C. A concentration of 0.004% was optimal for maintaining sperm quality in the short term (up to day 6), whereas 0.006% provided stronger protective effects during prolonged storage (up to day 10).
EVALUATION OF CREAM FORMULATION FROM NANOEMULSION Euphorbia tirucalli L. WITH STEARIC ACID VARIATIONS Meylani Nur Intan; Aldesra Fitri; Iqlima Ayu Prestisya
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 13 No. 1 (2026)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

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The macerate of Euphorbia tirucalli L. contains alkaloids, flavonoids, saponins, steroids, terpenoids, and tannins with antibacterial potential for acne management. This study formulated a nanoemulsion of E. tirucalli extract via the high-energy method, which was incorporated into cream formulations with varying stearic acid concentrations (F1;7.5%, F2;9%, F3;12%, F4;14.5%) and physical quality evaluation was carried out. The physical quality test of the cream preparations found that concentrations of stearic acid significantly affected the organoleptic properties (color and skin feels after application), spreadability 4.86-6.55 cm, water washability 8.5-19.1 mL, and viscosity 15557-31433 cPs. Meanwhile, homogeneity, phase separation, and pH of the preparation were not found the significantly different. An increase in stearic acid was directly proportional to washability and viscosity, but inversely proportional to spreadability. The formulations met the physical criteria for an acceptable cream preparation, except for pH, which was excessively alkaline. F2 and F3 establishing them as the most suitable formulations for further development. The antibacterial test against Propionibacterium acnes using the well diffusion agar assay, resulted; positive control (Mediklin; clindamycin 0.01%) in strong activity, 5% extract was in the weak activity, 10% and 20% extract was in moderate activity, 30% extract showed strong activity which is comparable to the positive control, and 20% nanoemulsion had no activity. The Spearman’s Rho test confirmed a strong correlation between extract concentration and antibacterial potency (r = 0.927).
CORRELATION OF BODY MORPHOMETRY AND UDDER VOLUME WITH MILK PRODUCTION IN ETTAWA CROSSBREED GOATS IN NUSA TENGGARA BARAT Ni Made Andry Kartika; Abyadul Fitriyah; Yuni Mariani; Nefi Andriana Fajri
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 13 No. 1 (2026)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

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Abstract

Observation of body size can be used as an identification of breeders as a selection criterion of dairy goats identify superior breeding stock and optimize milk production in Nusa Tenggara Barat, with potential implications for food security. The study aimed to determine the relationship between body size and udder volume with milk production of Etawa Crossbreed goat. This study was conducted at the experimental pen of Universitas Nahdlatul Wathan Mataram in Desa Ketangga Jeraeng, Lombok Timur, with twenty-five female Etawa Crossbreeds that were lactating. This study used a survey method and measurements of body length (BL), body height (BH), chest circumference (CC), udder volume (UV) and milk production (MP). Data were analyzed using correlation and linear regression analysis. The results showed that the average of BL = 72.76 cm, BH = 66.6 cm, CC = 73.24 cm, UV = 30.31 liters and MP = 327.76 ml / day. Body length and udder volume were moderately correlated with milk production (r = 0.40-0.59). While body height and udder volume had a strong correlation with milk production (r above 0.60). From the results, it can be concluded that body length, body height and udder volume had a significant effect on milk production, and there was a positive correlation with udder volume and milk production which was classified as a moderate correlation (r = 0.40-0.59).

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